Clinical significance of anti-DFS70 antibody in antinuclear antibody-positive samples with a dense fine speckled pattern
When the dense fine speckled (DFS) pattern-antinuclear antibodies (ANA) are detected in the indirect immunofluorescence (IIF) assay, the presence of anti-dense fine speckles 70 (DFS70) antibodies has been suggested to facilitate the exclusion of ANA-associated rheumatic diseases (AARD). We evaluated...
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| Veröffentlicht in: | Journal of laboratory medicine 2019-06, Vol.43 (3), p.149-155 |
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| creator | Koo, Sun Hoe Kim, Jimyung Kim, Seon Young Kwon, Gye Cheol |
| description | When the dense fine speckled (DFS) pattern-antinuclear antibodies (ANA) are detected in the indirect immunofluorescence (IIF) assay, the presence of anti-dense fine speckles 70 (DFS70) antibodies has been suggested to facilitate the exclusion of ANA-associated rheumatic diseases (AARD). We evaluated the potential use of anti-DFS70 antibodies for verifying AARD in patients with a positive ANA result of the DFS pattern.A total of 5509 patients who were requested ANA testing were included. The DFS pattern was confirmed using two IIF assays. Semiquantitative DFS70 ELISA (Euroimmun, Germany) was examined in samples with the DFS pattern.Among 639 ANA-positive patients, 19.6% displayed the DFS pattern. And 17.6% of patients with the DFS pattern were diagnosed with AARD. The low titer of 1:80 was more prevalent in the non-AARD group than in the AARD group (64.1% vs. 4.5%, p |
| doi_str_mv | 10.1515/labmed-2019-0036 |
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We evaluated the potential use of anti-DFS70 antibodies for verifying AARD in patients with a positive ANA result of the DFS pattern.A total of 5509 patients who were requested ANA testing were included. The DFS pattern was confirmed using two IIF assays. Semiquantitative DFS70 ELISA (Euroimmun, Germany) was examined in samples with the DFS pattern.Among 639 ANA-positive patients, 19.6% displayed the DFS pattern. And 17.6% of patients with the DFS pattern were diagnosed with AARD. The low titer of 1:80 was more prevalent in the non-AARD group than in the AARD group (64.1% vs. 4.5%, p < 0.0001). Anti-DFS70 antibodies were positive in 60.0% of patients with the DFS pattern. The frequency of anti-DFS70 positivity was higher in the non-rheumatic disease (NRD) group (74.2%) than in the other rheumatic disease group (43.2%, p = 0.003) and the AARD group (45.5%, p = 0.019).The DFS pattern is present in both AARD and non-AARD cases. In the DFS pattern, a low titer of 1:80 and isolated anti-DFS70 antibodies without AARD-associated antibodies represent a low likelihood of AARD. The presence of anti-DFS70 antibodies cannot exclude AARD and should be analyzed in combination with AARD-associated antibodies in the diagnostic algorithm.</description><identifier>ISSN: 0342-3026</identifier><identifier>ISSN: 2567-9430</identifier><identifier>EISSN: 1439-0477</identifier><identifier>EISSN: 2567-9449</identifier><identifier>DOI: 10.1515/labmed-2019-0036</identifier><language>eng</language><publisher>Berlin: De Gruyter</publisher><subject>Algorithms ; ANA-associated rheumatic diseases ; Antibodies ; Antinuclear antibodies ; antinuclear antibody ; Clinical significance ; DFS70 ; Diagnostic systems ; Enzyme-linked immunosorbent assay ; Immunofluorescence ; Immunoglobulins ; indirect immunofluorescence ; Rheumatic diseases</subject><ispartof>Journal of laboratory medicine, 2019-06, Vol.43 (3), p.149-155</ispartof><rights>2019. This work is published under http://creativecommons.org/licenses/by-nc-nd/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-472baa4e8503ed7c1aabc9ab0f1a9d07732af3045ffabbe9704dc35f30fde6193</citedby><cites>FETCH-LOGICAL-c405t-472baa4e8503ed7c1aabc9ab0f1a9d07732af3045ffabbe9704dc35f30fde6193</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27915,27916</link.rule.ids></links><search><creatorcontrib>Koo, Sun Hoe</creatorcontrib><creatorcontrib>Kim, Jimyung</creatorcontrib><creatorcontrib>Kim, Seon Young</creatorcontrib><creatorcontrib>Kwon, Gye Cheol</creatorcontrib><title>Clinical significance of anti-DFS70 antibody in antinuclear antibody-positive samples with a dense fine speckled pattern</title><title>Journal of laboratory medicine</title><description>When the dense fine speckled (DFS) pattern-antinuclear antibodies (ANA) are detected in the indirect immunofluorescence (IIF) assay, the presence of anti-dense fine speckles 70 (DFS70) antibodies has been suggested to facilitate the exclusion of ANA-associated rheumatic diseases (AARD). We evaluated the potential use of anti-DFS70 antibodies for verifying AARD in patients with a positive ANA result of the DFS pattern.A total of 5509 patients who were requested ANA testing were included. The DFS pattern was confirmed using two IIF assays. Semiquantitative DFS70 ELISA (Euroimmun, Germany) was examined in samples with the DFS pattern.Among 639 ANA-positive patients, 19.6% displayed the DFS pattern. And 17.6% of patients with the DFS pattern were diagnosed with AARD. The low titer of 1:80 was more prevalent in the non-AARD group than in the AARD group (64.1% vs. 4.5%, p < 0.0001). Anti-DFS70 antibodies were positive in 60.0% of patients with the DFS pattern. The frequency of anti-DFS70 positivity was higher in the non-rheumatic disease (NRD) group (74.2%) than in the other rheumatic disease group (43.2%, p = 0.003) and the AARD group (45.5%, p = 0.019).The DFS pattern is present in both AARD and non-AARD cases. In the DFS pattern, a low titer of 1:80 and isolated anti-DFS70 antibodies without AARD-associated antibodies represent a low likelihood of AARD. The presence of anti-DFS70 antibodies cannot exclude AARD and should be analyzed in combination with AARD-associated antibodies in the diagnostic algorithm.</description><subject>Algorithms</subject><subject>ANA-associated rheumatic diseases</subject><subject>Antibodies</subject><subject>Antinuclear antibodies</subject><subject>antinuclear antibody</subject><subject>Clinical significance</subject><subject>DFS70</subject><subject>Diagnostic systems</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Immunofluorescence</subject><subject>Immunoglobulins</subject><subject>indirect immunofluorescence</subject><subject>Rheumatic diseases</subject><issn>0342-3026</issn><issn>2567-9430</issn><issn>1439-0477</issn><issn>2567-9449</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kEFv2zAMhYViA5plve8ooGe3lCVH8aXAkC3tgAA9tD0btERlyhzZk5yl-fdTkmE99cQP5Hsk-Bj7IuBGVKK67bDdki1KEHUBIGcXbCKUzKy0_sAmIFVZSChnl-xTShsAoesSJux10fngDXY8-XXwLmMwxHvHMYy--LZ80nDCtrcH7sOJw850hPF_vxj65Ef_h3jC7dBR4ns__uTILYVE3PmQJwOZXx1ZPuA4Ugyf2UeHXaKrf3XKXpbfnxcPxerx_sfi66owCqqxULpsERXNK5BktRGIramxBSewtqC1LNFJUJVz2LZUa1DWyCq3nKWZqOWUXZ_3DrH_vaM0Npt-F0M-2ZSlAjWfz_Usq-CsMrFPKZJrhui3GA-NgOaYb3POtznm2xzzzZa7s2WPXX7I0jruDhne9r9nVVIKVcu_skqGKQ</recordid><startdate>20190626</startdate><enddate>20190626</enddate><creator>Koo, Sun Hoe</creator><creator>Kim, Jimyung</creator><creator>Kim, Seon Young</creator><creator>Kwon, Gye Cheol</creator><general>De Gruyter</general><general>Walter de Gruyter GmbH</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20190626</creationdate><title>Clinical significance of anti-DFS70 antibody in antinuclear antibody-positive samples with a dense fine speckled pattern</title><author>Koo, Sun Hoe ; Kim, Jimyung ; Kim, Seon Young ; Kwon, Gye Cheol</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-472baa4e8503ed7c1aabc9ab0f1a9d07732af3045ffabbe9704dc35f30fde6193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Algorithms</topic><topic>ANA-associated rheumatic diseases</topic><topic>Antibodies</topic><topic>Antinuclear antibodies</topic><topic>antinuclear antibody</topic><topic>Clinical significance</topic><topic>DFS70</topic><topic>Diagnostic systems</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Immunofluorescence</topic><topic>Immunoglobulins</topic><topic>indirect immunofluorescence</topic><topic>Rheumatic diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koo, Sun Hoe</creatorcontrib><creatorcontrib>Kim, Jimyung</creatorcontrib><creatorcontrib>Kim, Seon Young</creatorcontrib><creatorcontrib>Kwon, Gye Cheol</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of laboratory medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koo, Sun Hoe</au><au>Kim, Jimyung</au><au>Kim, Seon Young</au><au>Kwon, Gye Cheol</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clinical significance of anti-DFS70 antibody in antinuclear antibody-positive samples with a dense fine speckled pattern</atitle><jtitle>Journal of laboratory medicine</jtitle><date>2019-06-26</date><risdate>2019</risdate><volume>43</volume><issue>3</issue><spage>149</spage><epage>155</epage><pages>149-155</pages><issn>0342-3026</issn><issn>2567-9430</issn><eissn>1439-0477</eissn><eissn>2567-9449</eissn><abstract>When the dense fine speckled (DFS) pattern-antinuclear antibodies (ANA) are detected in the indirect immunofluorescence (IIF) assay, the presence of anti-dense fine speckles 70 (DFS70) antibodies has been suggested to facilitate the exclusion of ANA-associated rheumatic diseases (AARD). We evaluated the potential use of anti-DFS70 antibodies for verifying AARD in patients with a positive ANA result of the DFS pattern.A total of 5509 patients who were requested ANA testing were included. The DFS pattern was confirmed using two IIF assays. Semiquantitative DFS70 ELISA (Euroimmun, Germany) was examined in samples with the DFS pattern.Among 639 ANA-positive patients, 19.6% displayed the DFS pattern. And 17.6% of patients with the DFS pattern were diagnosed with AARD. The low titer of 1:80 was more prevalent in the non-AARD group than in the AARD group (64.1% vs. 4.5%, p < 0.0001). Anti-DFS70 antibodies were positive in 60.0% of patients with the DFS pattern. The frequency of anti-DFS70 positivity was higher in the non-rheumatic disease (NRD) group (74.2%) than in the other rheumatic disease group (43.2%, p = 0.003) and the AARD group (45.5%, p = 0.019).The DFS pattern is present in both AARD and non-AARD cases. In the DFS pattern, a low titer of 1:80 and isolated anti-DFS70 antibodies without AARD-associated antibodies represent a low likelihood of AARD. The presence of anti-DFS70 antibodies cannot exclude AARD and should be analyzed in combination with AARD-associated antibodies in the diagnostic algorithm.</abstract><cop>Berlin</cop><pub>De Gruyter</pub><doi>10.1515/labmed-2019-0036</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Algorithms ANA-associated rheumatic diseases Antibodies Antinuclear antibodies antinuclear antibody Clinical significance DFS70 Diagnostic systems Enzyme-linked immunosorbent assay Immunofluorescence Immunoglobulins indirect immunofluorescence Rheumatic diseases |
| title | Clinical significance of anti-DFS70 antibody in antinuclear antibody-positive samples with a dense fine speckled pattern |
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