Effects of exosome-like vesicles on cumulus expansion in pigs in vitro
Cell-secreted vesicles, such as exosomes, have recently been recognized as mediators of cell communication. A recent study in cattle showed the involvement of exosome-like vesicles in the control of cumulus expansion, a prerequisite process for normal ovulation; however, whether this is the case in...
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Veröffentlicht in: | The Journal of reproduction and development 2017-01, Vol.63 (1), p.51 |
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creator | MATSUNO, Yuta ONUMA, Asuka FUJIOKA, Yoshie A YASUHARA, Kazuma FUJII, Wataru NAITO, Kunihiko SUGIURA, Koji |
description | Cell-secreted vesicles, such as exosomes, have recently been recognized as mediators of cell communication. A recent study in cattle showed the involvement of exosome-like vesicles in the control of cumulus expansion, a prerequisite process for normal ovulation; however, whether this is the case in other mammalian species is not known. Therefore, this study aimed to examine the presence of exosome-like vesicles in ovarian follicles and their effects on cumulus expansion in vitro in pigs. The presence of exosome-like vesicles in porcine follicular fluid (pFF) was confirmed by transmission electron microscopic observation, the detection of marker proteins, and RNA profiles specific to exosomes. Fluorescently labeled exosome-like vesicles isolated from pFF were incorporated into both cumulus and mural granulosa cells in vitro. Exosome-like vesicles were not capable of inducing cumulus expansion to a degree comparable to that induced by follicle-stimulating hormone (FSH). Moreover, exosome-like vesicles had no significant effects on the expression levels of transcripts required for the normal expansion process (HAS2, TNFAIP6, and PTGS2). Interestingly, FSH-induced expression of HAS2 and TNFAIP6 mRNA, but not of PTGS2 mRNA, was significantly increased by the presence of exosome-like vesicles; however, the degree of FSH-induced expansion was not affected. In addition, porcine exosome-like vesicles had no significant effects on the expansion of mouse cumulus-oocyte complexes. Collectively, the present results suggest that exosome-like vesicles are present in pFF, but they are not efficient in inducing cumulus expansion in pigs. |
doi_str_mv | 10.1262/jrd.2016-124 |
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A recent study in cattle showed the involvement of exosome-like vesicles in the control of cumulus expansion, a prerequisite process for normal ovulation; however, whether this is the case in other mammalian species is not known. Therefore, this study aimed to examine the presence of exosome-like vesicles in ovarian follicles and their effects on cumulus expansion in vitro in pigs. The presence of exosome-like vesicles in porcine follicular fluid (pFF) was confirmed by transmission electron microscopic observation, the detection of marker proteins, and RNA profiles specific to exosomes. Fluorescently labeled exosome-like vesicles isolated from pFF were incorporated into both cumulus and mural granulosa cells in vitro. Exosome-like vesicles were not capable of inducing cumulus expansion to a degree comparable to that induced by follicle-stimulating hormone (FSH). Moreover, exosome-like vesicles had no significant effects on the expression levels of transcripts required for the normal expansion process (HAS2, TNFAIP6, and PTGS2). Interestingly, FSH-induced expression of HAS2 and TNFAIP6 mRNA, but not of PTGS2 mRNA, was significantly increased by the presence of exosome-like vesicles; however, the degree of FSH-induced expansion was not affected. In addition, porcine exosome-like vesicles had no significant effects on the expansion of mouse cumulus-oocyte complexes. Collectively, the present results suggest that exosome-like vesicles are present in pFF, but they are not efficient in inducing cumulus expansion in pigs.</description><identifier>ISSN: 0916-8818</identifier><identifier>EISSN: 1348-4400</identifier><identifier>DOI: 10.1262/jrd.2016-124</identifier><language>eng ; jpn</language><publisher>Tokyo: Japan Science and Technology Agency</publisher><subject>Cell interactions ; Exosomes ; Follicle-stimulating hormone ; Follicles ; Follicular fluid ; Granulosa cells ; Ovulation ; Vesicles</subject><ispartof>The Journal of reproduction and development, 2017-01, Vol.63 (1), p.51</ispartof><rights>Copyright Japan Science and Technology Agency 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids></links><search><creatorcontrib>MATSUNO, Yuta</creatorcontrib><creatorcontrib>ONUMA, Asuka</creatorcontrib><creatorcontrib>FUJIOKA, Yoshie A</creatorcontrib><creatorcontrib>YASUHARA, Kazuma</creatorcontrib><creatorcontrib>FUJII, Wataru</creatorcontrib><creatorcontrib>NAITO, Kunihiko</creatorcontrib><creatorcontrib>SUGIURA, Koji</creatorcontrib><title>Effects of exosome-like vesicles on cumulus expansion in pigs in vitro</title><title>The Journal of reproduction and development</title><description>Cell-secreted vesicles, such as exosomes, have recently been recognized as mediators of cell communication. A recent study in cattle showed the involvement of exosome-like vesicles in the control of cumulus expansion, a prerequisite process for normal ovulation; however, whether this is the case in other mammalian species is not known. Therefore, this study aimed to examine the presence of exosome-like vesicles in ovarian follicles and their effects on cumulus expansion in vitro in pigs. The presence of exosome-like vesicles in porcine follicular fluid (pFF) was confirmed by transmission electron microscopic observation, the detection of marker proteins, and RNA profiles specific to exosomes. Fluorescently labeled exosome-like vesicles isolated from pFF were incorporated into both cumulus and mural granulosa cells in vitro. Exosome-like vesicles were not capable of inducing cumulus expansion to a degree comparable to that induced by follicle-stimulating hormone (FSH). Moreover, exosome-like vesicles had no significant effects on the expression levels of transcripts required for the normal expansion process (HAS2, TNFAIP6, and PTGS2). Interestingly, FSH-induced expression of HAS2 and TNFAIP6 mRNA, but not of PTGS2 mRNA, was significantly increased by the presence of exosome-like vesicles; however, the degree of FSH-induced expansion was not affected. In addition, porcine exosome-like vesicles had no significant effects on the expansion of mouse cumulus-oocyte complexes. Collectively, the present results suggest that exosome-like vesicles are present in pFF, but they are not efficient in inducing cumulus expansion in pigs.</description><subject>Cell interactions</subject><subject>Exosomes</subject><subject>Follicle-stimulating hormone</subject><subject>Follicles</subject><subject>Follicular fluid</subject><subject>Granulosa cells</subject><subject>Ovulation</subject><subject>Vesicles</subject><issn>0916-8818</issn><issn>1348-4400</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNotj01LxDAYhIMoWNa9-QMKnrPmzUfbHGXZVWHBi56Xpnkjqd2mNs3izzfingaeGWYYQu6BbYBX_LGf7YYzqChweUUKELKhUjJ2TQqmM24aaG7JOkZvmJS1VEqJgux3zmG3xDK4En9CDCekg__C8ozRdwNmYyy7dEpDijkwtWP0mfixnPxn_NOzX-ZwR25cO0RcX3RFPva79-0LPbw9v26fDrQH4IIaZbnSlmktWwkajbQga6MMMIGVVtaiQnAguKsVGseVa1sFoqud7RqoxIo8_PdOc_hOGJdjH9I85skj50JXXORX4hd6VE4O</recordid><startdate>20170101</startdate><enddate>20170101</enddate><creator>MATSUNO, Yuta</creator><creator>ONUMA, Asuka</creator><creator>FUJIOKA, Yoshie A</creator><creator>YASUHARA, Kazuma</creator><creator>FUJII, Wataru</creator><creator>NAITO, Kunihiko</creator><creator>SUGIURA, Koji</creator><general>Japan Science and Technology Agency</general><scope>7T5</scope><scope>7TK</scope><scope>H94</scope></search><sort><creationdate>20170101</creationdate><title>Effects of exosome-like vesicles on cumulus expansion in pigs in vitro</title><author>MATSUNO, Yuta ; ONUMA, Asuka ; FUJIOKA, Yoshie A ; YASUHARA, Kazuma ; FUJII, Wataru ; NAITO, Kunihiko ; SUGIURA, Koji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j1123-b5d259d0994a419eb4d147b5b103e695dde5e1f132f75ebf25faa513c7fdc8163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng ; jpn</language><creationdate>2017</creationdate><topic>Cell interactions</topic><topic>Exosomes</topic><topic>Follicle-stimulating hormone</topic><topic>Follicles</topic><topic>Follicular fluid</topic><topic>Granulosa cells</topic><topic>Ovulation</topic><topic>Vesicles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MATSUNO, Yuta</creatorcontrib><creatorcontrib>ONUMA, Asuka</creatorcontrib><creatorcontrib>FUJIOKA, Yoshie A</creatorcontrib><creatorcontrib>YASUHARA, Kazuma</creatorcontrib><creatorcontrib>FUJII, Wataru</creatorcontrib><creatorcontrib>NAITO, Kunihiko</creatorcontrib><creatorcontrib>SUGIURA, Koji</creatorcontrib><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>The Journal of reproduction and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MATSUNO, Yuta</au><au>ONUMA, Asuka</au><au>FUJIOKA, Yoshie A</au><au>YASUHARA, Kazuma</au><au>FUJII, Wataru</au><au>NAITO, Kunihiko</au><au>SUGIURA, Koji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of exosome-like vesicles on cumulus expansion in pigs in vitro</atitle><jtitle>The Journal of reproduction and development</jtitle><date>2017-01-01</date><risdate>2017</risdate><volume>63</volume><issue>1</issue><spage>51</spage><pages>51-</pages><issn>0916-8818</issn><eissn>1348-4400</eissn><abstract>Cell-secreted vesicles, such as exosomes, have recently been recognized as mediators of cell communication. A recent study in cattle showed the involvement of exosome-like vesicles in the control of cumulus expansion, a prerequisite process for normal ovulation; however, whether this is the case in other mammalian species is not known. Therefore, this study aimed to examine the presence of exosome-like vesicles in ovarian follicles and their effects on cumulus expansion in vitro in pigs. The presence of exosome-like vesicles in porcine follicular fluid (pFF) was confirmed by transmission electron microscopic observation, the detection of marker proteins, and RNA profiles specific to exosomes. Fluorescently labeled exosome-like vesicles isolated from pFF were incorporated into both cumulus and mural granulosa cells in vitro. Exosome-like vesicles were not capable of inducing cumulus expansion to a degree comparable to that induced by follicle-stimulating hormone (FSH). Moreover, exosome-like vesicles had no significant effects on the expression levels of transcripts required for the normal expansion process (HAS2, TNFAIP6, and PTGS2). Interestingly, FSH-induced expression of HAS2 and TNFAIP6 mRNA, but not of PTGS2 mRNA, was significantly increased by the presence of exosome-like vesicles; however, the degree of FSH-induced expansion was not affected. In addition, porcine exosome-like vesicles had no significant effects on the expansion of mouse cumulus-oocyte complexes. Collectively, the present results suggest that exosome-like vesicles are present in pFF, but they are not efficient in inducing cumulus expansion in pigs.</abstract><cop>Tokyo</cop><pub>Japan Science and Technology Agency</pub><doi>10.1262/jrd.2016-124</doi></addata></record> |
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subjects | Cell interactions Exosomes Follicle-stimulating hormone Follicles Follicular fluid Granulosa cells Ovulation Vesicles |
title | Effects of exosome-like vesicles on cumulus expansion in pigs in vitro |
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