Magnetic Fe3O4@mTiO2-AIPA Microspheres for Separation of Phosphoproteins and Non-phosphoproteins
A novel phosphoprotein separation material was developed, which is constructed by a magnetic mesoporous Fe 3 O 4 @TiO 2 (Fe 3 O 4 @mTiO 2 ) microsphere and a 5-aminoisophthalic acid (AIPA) monolayer that provides additional binding sites toward phosphate groups. The results of characteristic experim...
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| Veröffentlicht in: | Journal of Wuhan University of Technology. Materials science edition 2019-06, Vol.34 (3), p.752-759 |
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| container_title | Journal of Wuhan University of Technology. Materials science edition |
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| creator | Tang, Qiuhan Zhao, Rui Lu, Qi Qing, Guangyan |
| description | A novel phosphoprotein separation material was developed, which is constructed by a magnetic mesoporous Fe
3
O
4
@TiO
2
(Fe
3
O
4
@mTiO
2
) microsphere and a 5-aminoisophthalic acid (AIPA) monolayer that provides additional binding sites toward phosphate groups. The results of characteristic experiments demonstrated that Fe
3
O
4
@mTiO
2
-AIPA had good dispersability, high magnetic susceptibility, and satisfactory grafting ratio of AIPA, ascribed to the large specific surface area of the inorganic substrate. Taking advantages of these features, Fe
3
O
4
@mTiO
2
-AIPA was successfully utilized to separate
α
-casein (a typical phosphoprotein) and bovine serum albumin (BSA, a typical non-phosphoprotein) from their mixtures (molar ratio = 1:2). Through adjusting pH and polarity of solutions, the BSA and
α
-casein were respectively enriched in washing fraction and elution fraction. This result displays the good potential of Fe
3
O
4
@mTiO
2
-AIPA for application in phosphoprotein enrichment. |
| doi_str_mv | 10.1007/s11595-019-2113-z |
| format | Article |
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3
O
4
@TiO
2
(Fe
3
O
4
@mTiO
2
) microsphere and a 5-aminoisophthalic acid (AIPA) monolayer that provides additional binding sites toward phosphate groups. The results of characteristic experiments demonstrated that Fe
3
O
4
@mTiO
2
-AIPA had good dispersability, high magnetic susceptibility, and satisfactory grafting ratio of AIPA, ascribed to the large specific surface area of the inorganic substrate. Taking advantages of these features, Fe
3
O
4
@mTiO
2
-AIPA was successfully utilized to separate
α
-casein (a typical phosphoprotein) and bovine serum albumin (BSA, a typical non-phosphoprotein) from their mixtures (molar ratio = 1:2). Through adjusting pH and polarity of solutions, the BSA and
α
-casein were respectively enriched in washing fraction and elution fraction. This result displays the good potential of Fe
3
O
4
@mTiO
2
-AIPA for application in phosphoprotein enrichment.</description><identifier>ISSN: 1000-2413</identifier><identifier>EISSN: 1993-0437</identifier><identifier>DOI: 10.1007/s11595-019-2113-z</identifier><language>eng</language><publisher>Wuhan: Wuhan University of Technology</publisher><subject>Binding sites ; Biomaterials ; Casein ; Chemistry and Materials Science ; Elution ; Iron oxides ; Magnetic permeability ; Materials Science ; Microspheres ; Polarity ; Separation ; Serum albumin ; Substrates ; Titanium dioxide</subject><ispartof>Journal of Wuhan University of Technology. Materials science edition, 2019-06, Vol.34 (3), p.752-759</ispartof><rights>Wuhan University of Technology and Springer-Verlag GmbH Germany, Part of Springer Nature 2019</rights><rights>Copyright Springer Nature B.V. 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c183z-1c8284b10fd9268e8c598973f53401cc358a46b34d8512426314910f61cd0c3b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11595-019-2113-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11595-019-2113-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27913,27914,41477,42546,51308</link.rule.ids></links><search><creatorcontrib>Tang, Qiuhan</creatorcontrib><creatorcontrib>Zhao, Rui</creatorcontrib><creatorcontrib>Lu, Qi</creatorcontrib><creatorcontrib>Qing, Guangyan</creatorcontrib><title>Magnetic Fe3O4@mTiO2-AIPA Microspheres for Separation of Phosphoproteins and Non-phosphoproteins</title><title>Journal of Wuhan University of Technology. Materials science edition</title><addtitle>J. Wuhan Univ. Technol.-Mat. Sci. Edit</addtitle><description>A novel phosphoprotein separation material was developed, which is constructed by a magnetic mesoporous Fe
3
O
4
@TiO
2
(Fe
3
O
4
@mTiO
2
) microsphere and a 5-aminoisophthalic acid (AIPA) monolayer that provides additional binding sites toward phosphate groups. The results of characteristic experiments demonstrated that Fe
3
O
4
@mTiO
2
-AIPA had good dispersability, high magnetic susceptibility, and satisfactory grafting ratio of AIPA, ascribed to the large specific surface area of the inorganic substrate. Taking advantages of these features, Fe
3
O
4
@mTiO
2
-AIPA was successfully utilized to separate
α
-casein (a typical phosphoprotein) and bovine serum albumin (BSA, a typical non-phosphoprotein) from their mixtures (molar ratio = 1:2). Through adjusting pH and polarity of solutions, the BSA and
α
-casein were respectively enriched in washing fraction and elution fraction. This result displays the good potential of Fe
3
O
4
@mTiO
2
-AIPA for application in phosphoprotein enrichment.</description><subject>Binding sites</subject><subject>Biomaterials</subject><subject>Casein</subject><subject>Chemistry and Materials Science</subject><subject>Elution</subject><subject>Iron oxides</subject><subject>Magnetic permeability</subject><subject>Materials Science</subject><subject>Microspheres</subject><subject>Polarity</subject><subject>Separation</subject><subject>Serum albumin</subject><subject>Substrates</subject><subject>Titanium dioxide</subject><issn>1000-2413</issn><issn>1993-0437</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kEFPwzAMhSsEEmPwA7hF4hyI47RLbkwTg0mDTWKcQ5emWyfWlKQ7sF9PqiIhDpxs2d_zk1-SXAO7BcZGdwEgVSlloCgHQHo8SQagFFImcHQae8YY5QLwPLkIYceYYJhlg-T9Od_Utq0MmVpciPv9qlpwOp4tx-S5Mt6FZmu9DaR0nrzaJvd5W7mauJIst93SNd61tqoDyeuCvLiaNn_nl8lZmX8Ee_VTh8nb9GE1eaLzxeNsMp5TAxKPFIzkUqyBlYXimbTSpEqqEZYpCgbGYCpzka1RFDIFLniGIFSkMzAFM7jGYXLT343GnwcbWr1zB19HS805SsZRIEQKeqp7LXhb6sZX-9x_aWC6C1L3QeoYpO6C1Meo4b0mRLbeWP97-X_RN98gdQI</recordid><startdate>20190601</startdate><enddate>20190601</enddate><creator>Tang, Qiuhan</creator><creator>Zhao, Rui</creator><creator>Lu, Qi</creator><creator>Qing, Guangyan</creator><general>Wuhan University of Technology</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope></search><sort><creationdate>20190601</creationdate><title>Magnetic Fe3O4@mTiO2-AIPA Microspheres for Separation of Phosphoproteins and Non-phosphoproteins</title><author>Tang, Qiuhan ; Zhao, Rui ; Lu, Qi ; Qing, Guangyan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c183z-1c8284b10fd9268e8c598973f53401cc358a46b34d8512426314910f61cd0c3b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Binding sites</topic><topic>Biomaterials</topic><topic>Casein</topic><topic>Chemistry and Materials Science</topic><topic>Elution</topic><topic>Iron oxides</topic><topic>Magnetic permeability</topic><topic>Materials Science</topic><topic>Microspheres</topic><topic>Polarity</topic><topic>Separation</topic><topic>Serum albumin</topic><topic>Substrates</topic><topic>Titanium dioxide</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tang, Qiuhan</creatorcontrib><creatorcontrib>Zhao, Rui</creatorcontrib><creatorcontrib>Lu, Qi</creatorcontrib><creatorcontrib>Qing, Guangyan</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><jtitle>Journal of Wuhan University of Technology. Materials science edition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tang, Qiuhan</au><au>Zhao, Rui</au><au>Lu, Qi</au><au>Qing, Guangyan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Magnetic Fe3O4@mTiO2-AIPA Microspheres for Separation of Phosphoproteins and Non-phosphoproteins</atitle><jtitle>Journal of Wuhan University of Technology. Materials science edition</jtitle><stitle>J. Wuhan Univ. Technol.-Mat. Sci. Edit</stitle><date>2019-06-01</date><risdate>2019</risdate><volume>34</volume><issue>3</issue><spage>752</spage><epage>759</epage><pages>752-759</pages><issn>1000-2413</issn><eissn>1993-0437</eissn><abstract>A novel phosphoprotein separation material was developed, which is constructed by a magnetic mesoporous Fe
3
O
4
@TiO
2
(Fe
3
O
4
@mTiO
2
) microsphere and a 5-aminoisophthalic acid (AIPA) monolayer that provides additional binding sites toward phosphate groups. The results of characteristic experiments demonstrated that Fe
3
O
4
@mTiO
2
-AIPA had good dispersability, high magnetic susceptibility, and satisfactory grafting ratio of AIPA, ascribed to the large specific surface area of the inorganic substrate. Taking advantages of these features, Fe
3
O
4
@mTiO
2
-AIPA was successfully utilized to separate
α
-casein (a typical phosphoprotein) and bovine serum albumin (BSA, a typical non-phosphoprotein) from their mixtures (molar ratio = 1:2). Through adjusting pH and polarity of solutions, the BSA and
α
-casein were respectively enriched in washing fraction and elution fraction. This result displays the good potential of Fe
3
O
4
@mTiO
2
-AIPA for application in phosphoprotein enrichment.</abstract><cop>Wuhan</cop><pub>Wuhan University of Technology</pub><doi>10.1007/s11595-019-2113-z</doi><tpages>8</tpages></addata></record> |
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| ispartof | Journal of Wuhan University of Technology. Materials science edition, 2019-06, Vol.34 (3), p.752-759 |
| issn | 1000-2413 1993-0437 |
| language | eng |
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| source | Springer Nature - Complete Springer Journals; Alma/SFX Local Collection |
| subjects | Binding sites Biomaterials Casein Chemistry and Materials Science Elution Iron oxides Magnetic permeability Materials Science Microspheres Polarity Separation Serum albumin Substrates Titanium dioxide |
| title | Magnetic Fe3O4@mTiO2-AIPA Microspheres for Separation of Phosphoproteins and Non-phosphoproteins |
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