Random Mutagenesis‐Driven Improvement of Carboxylate Reductase Activity using an Amino Benzamidoxime‐Mediated High‐Throughput Assay

Carboxylic acid reductases (CARs) catalyze the direct adenosine triphosphate (ATP) and nicotinamide adenine dinucleotide phosphate (NADPH) dependent reduction of carboxylic acids to their corresponding aldehydes. The identification and improvement of CARs by protein engineering is, however, severely...

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Veröffentlicht in:	Advanced synthesis & catalysis 2019-06, Vol.361 (11), p.2544-2549
Hauptverfasser:	Schwendenwein, Daniel, Ressmann, Anna K., Doerr, Mark, Höhne, Matthias, Bornscheuer, Uwe T., Mihovilovic, Marko D., Rudroff, Florian, Winkler, Margit
Format:	Artikel
Sprache:	eng
Schlagworte:	Acids Adenosine triphosphate aldehyde Aldehydes Aliphatic compounds amino benzamidoxime Benzoic acid carboxylic acid reductase (CAR) Carboxylic acids Catalysis Glutamine high-throughput screening Microorganisms Mutagenesis Mutation Nicotinamide Nicotinamide adenine dinucleotide Octanoic acid Proline Reductases Substitutes Substrates
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