Prevalence of Antibiotic Resistance and Distribution of Virulence Factors in the Shiga Toxigenic Escherichia coli Recovered from Hospital Food
Background: Regarding the presence of immunosuppressed patients in hospitals, hospital food must have a boost safety. Escherichia coli O157 is one of the prevalent causes of food-related poisoning.Objectives: The current examination was done to assess the distribution of virulence factors and phenot...
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| Veröffentlicht in: | Jundishapur journal of microbiology 2019-05, Vol.In Press (In Press), p.1-8 |
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| creator | Ranjbar, Reza Seyf, Ali Safarpoor Dehkordi, Farhad |
| description | Background: Regarding the presence of immunosuppressed patients in hospitals, hospital food must have a boost safety. Escherichia coli O157 is one of the prevalent causes of food-related poisoning.Objectives: The current examination was done to assess the distribution of virulence factors and phenotypic analysis of antibiotic resistance of E. coli O157 bacteria recovered from hospital food.Methods: From April to August 2016, 200 hospital food samples were obtained and directly transported to the laboratory. Escherichia coli O157-positive bacteria were analyzed by disk diffusion and polymerase chain reaction (PCR).Results: Nine out of 200 (4.50%) samples harbored E. coli O157. Distribution of E. coli O157 in soup and gavage samples were 3% and 6%, respectively. Stx1 (100%), eaeA (100%), and ehlyA (100%) were the most frequently detected virulence genes. Escherichia coli O157 bacteria exhibited maximum prevalence of antibiotic resistance against tetracycline (100%), gentamycin (100%), ampicillin (100%), mezlocillin (50%), enrofloxacin (50%), and trimethoprim (50%). Distribution of resistance of E. coli O157 bacteria against more than six antibiotic agents was 11.11%.Conclusions: Gavage and soup samples may be sources of virulent and resistant E. coli O157. High presence of E. coli O157, simultaneous presence of multiple virulence genes, and resistance against animal-based antibiotics presented inadequacy of cooking time and temperature in processing of hospital foods. |
| doi_str_mv | 10.5812/jjm.82659 |
| format | Article |
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Escherichia coli O157 is one of the prevalent causes of food-related poisoning.Objectives: The current examination was done to assess the distribution of virulence factors and phenotypic analysis of antibiotic resistance of E. coli O157 bacteria recovered from hospital food.Methods: From April to August 2016, 200 hospital food samples were obtained and directly transported to the laboratory. Escherichia coli O157-positive bacteria were analyzed by disk diffusion and polymerase chain reaction (PCR).Results: Nine out of 200 (4.50%) samples harbored E. coli O157. Distribution of E. coli O157 in soup and gavage samples were 3% and 6%, respectively. Stx1 (100%), eaeA (100%), and ehlyA (100%) were the most frequently detected virulence genes. Escherichia coli O157 bacteria exhibited maximum prevalence of antibiotic resistance against tetracycline (100%), gentamycin (100%), ampicillin (100%), mezlocillin (50%), enrofloxacin (50%), and trimethoprim (50%). Distribution of resistance of E. coli O157 bacteria against more than six antibiotic agents was 11.11%.Conclusions: Gavage and soup samples may be sources of virulent and resistant E. coli O157. High presence of E. coli O157, simultaneous presence of multiple virulence genes, and resistance against animal-based antibiotics presented inadequacy of cooking time and temperature in processing of hospital foods.</description><identifier>ISSN: 2008-3645</identifier><identifier>EISSN: 2008-4161</identifier><identifier>DOI: 10.5812/jjm.82659</identifier><language>eng</language><publisher>Ahvaz: Ahvaz Jundishapur University of Medical Sciences</publisher><subject>Ampicillin ; Antibiotic resistance ; Antibiotics ; Antimicrobial agents ; Bacteria ; Cooking ; Deoxyribonucleic acid ; DNA ; Drug resistance ; E coli ; Enrofloxacin ; Escherichia coli ; Food ; Food poisoning ; Food processing ; Laboratories ; Mezlocillin ; Milk ; Polymerase chain reaction ; Staphylococcus infections ; Statistical analysis ; Trimethoprim ; Virulence factors</subject><ispartof>Jundishapur journal of microbiology, 2019-05, Vol.In Press (In Press), p.1-8</ispartof><rights>2019. This work is published under https://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c292t-b4b0a64987be47ea908ff4c792cb204eab54a7ca49b67c969e37839be330ac173</citedby><cites>FETCH-LOGICAL-c292t-b4b0a64987be47ea908ff4c792cb204eab54a7ca49b67c969e37839be330ac173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids></links><search><creatorcontrib>Ranjbar, Reza</creatorcontrib><creatorcontrib>Seyf, Ali</creatorcontrib><creatorcontrib>Safarpoor Dehkordi, Farhad</creatorcontrib><title>Prevalence of Antibiotic Resistance and Distribution of Virulence Factors in the Shiga Toxigenic Escherichia coli Recovered from Hospital Food</title><title>Jundishapur journal of microbiology</title><description>Background: Regarding the presence of immunosuppressed patients in hospitals, hospital food must have a boost safety. Escherichia coli O157 is one of the prevalent causes of food-related poisoning.Objectives: The current examination was done to assess the distribution of virulence factors and phenotypic analysis of antibiotic resistance of E. coli O157 bacteria recovered from hospital food.Methods: From April to August 2016, 200 hospital food samples were obtained and directly transported to the laboratory. Escherichia coli O157-positive bacteria were analyzed by disk diffusion and polymerase chain reaction (PCR).Results: Nine out of 200 (4.50%) samples harbored E. coli O157. Distribution of E. coli O157 in soup and gavage samples were 3% and 6%, respectively. Stx1 (100%), eaeA (100%), and ehlyA (100%) were the most frequently detected virulence genes. Escherichia coli O157 bacteria exhibited maximum prevalence of antibiotic resistance against tetracycline (100%), gentamycin (100%), ampicillin (100%), mezlocillin (50%), enrofloxacin (50%), and trimethoprim (50%). Distribution of resistance of E. coli O157 bacteria against more than six antibiotic agents was 11.11%.Conclusions: Gavage and soup samples may be sources of virulent and resistant E. coli O157. High presence of E. coli O157, simultaneous presence of multiple virulence genes, and resistance against animal-based antibiotics presented inadequacy of cooking time and temperature in processing of hospital foods.</description><subject>Ampicillin</subject><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>Bacteria</subject><subject>Cooking</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Drug resistance</subject><subject>E coli</subject><subject>Enrofloxacin</subject><subject>Escherichia coli</subject><subject>Food</subject><subject>Food poisoning</subject><subject>Food processing</subject><subject>Laboratories</subject><subject>Mezlocillin</subject><subject>Milk</subject><subject>Polymerase chain reaction</subject><subject>Staphylococcus infections</subject><subject>Statistical analysis</subject><subject>Trimethoprim</subject><subject>Virulence factors</subject><issn>2008-3645</issn><issn>2008-4161</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNotkEFOwzAQRS0EElXpghtYYsUixXGcOF5WpaVIlUBQ2Ea2O2kcpXGxnQouwZlJaGcz8zV_3kgfoduYTNM8pg91vZ_mNEvFBRpRQvKIxVl8eZ6TjKXXaOJ9TYbiJGd0hH5fHRxlA60GbEs8a4NRxgaj8Rt444McFrLd4sdeOKO6YGw7OD-N605nS6mDdR6bFocK8HtldhJv7LfZQdtzFl5X4IyujMTaNqYHa3sEB1tcOrvHK-sPJsgGL63d3qCrUjYeJuc-Rh_LxWa-itYvT8_z2TrSVNAQKaaIzJjIuQLGQQqSlyXTXFCtKGEgVcok15IJlXEtMgEJzxOhIEmI1DFPxujuxD04-9WBD0VtO9f2LwtKEyaEoCLvXfcnl3bWewdlcXBmL91PEZNiSLzoEy_-E0_-AAKLdao</recordid><startdate>20190501</startdate><enddate>20190501</enddate><creator>Ranjbar, Reza</creator><creator>Seyf, Ali</creator><creator>Safarpoor Dehkordi, Farhad</creator><general>Ahvaz Jundishapur University of Medical Sciences</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20190501</creationdate><title>Prevalence of Antibiotic Resistance and Distribution of Virulence Factors in the Shiga Toxigenic Escherichia coli Recovered from Hospital Food</title><author>Ranjbar, Reza ; Seyf, Ali ; Safarpoor Dehkordi, Farhad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c292t-b4b0a64987be47ea908ff4c792cb204eab54a7ca49b67c969e37839be330ac173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Ampicillin</topic><topic>Antibiotic resistance</topic><topic>Antibiotics</topic><topic>Antimicrobial agents</topic><topic>Bacteria</topic><topic>Cooking</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Drug resistance</topic><topic>E coli</topic><topic>Enrofloxacin</topic><topic>Escherichia coli</topic><topic>Food</topic><topic>Food poisoning</topic><topic>Food processing</topic><topic>Laboratories</topic><topic>Mezlocillin</topic><topic>Milk</topic><topic>Polymerase chain reaction</topic><topic>Staphylococcus infections</topic><topic>Statistical analysis</topic><topic>Trimethoprim</topic><topic>Virulence factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ranjbar, Reza</creatorcontrib><creatorcontrib>Seyf, Ali</creatorcontrib><creatorcontrib>Safarpoor Dehkordi, Farhad</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>Middle East & Africa Database</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Jundishapur journal of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ranjbar, Reza</au><au>Seyf, Ali</au><au>Safarpoor Dehkordi, Farhad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prevalence of Antibiotic Resistance and Distribution of Virulence Factors in the Shiga Toxigenic Escherichia coli Recovered from Hospital Food</atitle><jtitle>Jundishapur journal of microbiology</jtitle><date>2019-05-01</date><risdate>2019</risdate><volume>In Press</volume><issue>In Press</issue><spage>1</spage><epage>8</epage><pages>1-8</pages><issn>2008-3645</issn><eissn>2008-4161</eissn><abstract>Background: Regarding the presence of immunosuppressed patients in hospitals, hospital food must have a boost safety. Escherichia coli O157 is one of the prevalent causes of food-related poisoning.Objectives: The current examination was done to assess the distribution of virulence factors and phenotypic analysis of antibiotic resistance of E. coli O157 bacteria recovered from hospital food.Methods: From April to August 2016, 200 hospital food samples were obtained and directly transported to the laboratory. Escherichia coli O157-positive bacteria were analyzed by disk diffusion and polymerase chain reaction (PCR).Results: Nine out of 200 (4.50%) samples harbored E. coli O157. Distribution of E. coli O157 in soup and gavage samples were 3% and 6%, respectively. Stx1 (100%), eaeA (100%), and ehlyA (100%) were the most frequently detected virulence genes. Escherichia coli O157 bacteria exhibited maximum prevalence of antibiotic resistance against tetracycline (100%), gentamycin (100%), ampicillin (100%), mezlocillin (50%), enrofloxacin (50%), and trimethoprim (50%). Distribution of resistance of E. coli O157 bacteria against more than six antibiotic agents was 11.11%.Conclusions: Gavage and soup samples may be sources of virulent and resistant E. coli O157. High presence of E. coli O157, simultaneous presence of multiple virulence genes, and resistance against animal-based antibiotics presented inadequacy of cooking time and temperature in processing of hospital foods.</abstract><cop>Ahvaz</cop><pub>Ahvaz Jundishapur University of Medical Sciences</pub><doi>10.5812/jjm.82659</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Ampicillin Antibiotic resistance Antibiotics Antimicrobial agents Bacteria Cooking Deoxyribonucleic acid DNA Drug resistance E coli Enrofloxacin Escherichia coli Food Food poisoning Food processing Laboratories Mezlocillin Milk Polymerase chain reaction Staphylococcus infections Statistical analysis Trimethoprim Virulence factors |
| title | Prevalence of Antibiotic Resistance and Distribution of Virulence Factors in the Shiga Toxigenic Escherichia coli Recovered from Hospital Food |
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