Characterization of the gene encoding 4-coumarate:CoA ligase in Coleus forskohlii
4-coumarate:coenzyme A ligase (4CL) converts 4-coumaric acid and its hydroxylated derivatives into the CoA thiol esters, directing carbon flux into various end-products of phenylpropanoid metabolism, such as flavonoids and lignins. In this study, full-length cDNA showing homology with plant 4CL gene...
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| Veröffentlicht in: | Journal of plant biochemistry and biotechnology 2019-06, Vol.28 (2), p.203-210 |
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| creator | Awasthi, Praveen Mahajan, Vidushi Jamwal, Vijay Lakshmi Chouhan, Rekha Kapoor, Nitika Bedi, Yashbir S. Gandhi, Sumit G. |
| description | 4-coumarate:coenzyme A ligase (4CL) converts 4-coumaric acid and its hydroxylated derivatives into the CoA thiol esters, directing carbon flux into various end-products of phenylpropanoid metabolism, such as flavonoids and lignins. In this study, full-length cDNA showing homology with plant
4CL
genes was cloned from
Coleus forskohlii
and was designated as
Cf4CL
(Accession No. KF643242).
Cf4CL
was found to contain an ORF of 1626 bps. The computational translation of
Cf4CL
encoded a protein of 541 amino acids. Theoretical isoelectric point and molecular weight of
Cf
4CL were calculated to be 5.55 and 58.65 kDa, respectively. Phylogenetic tree clustered
Cf4CL
with the class I 4CLs (involved in lignin biosynthesis). Spatial distribution of
Cf4CL
in different tissues and its expression in response to various stresses was carried out through qPCR. Abscisic acid (ABA) treatment strongly induced the expression of
Cf4CL
. Homology modeling and docking studies further ascertain the role of
Cf4CL
gene in lignin biosynthesis. In silico prediction suggested that
Cf4CL
may be post-transcriptionally regulated by microRNAs. Decreased expression of miR1886 in response to ABA treatment was associated with an increase in
Cf4CL
transcripts and lignin content, thus suggesting a possible role of miR1886 in regulating lignin biosynthesis in
C. forskohlii
. |
| doi_str_mv | 10.1007/s13562-018-0468-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2233151480</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2233151480</sourcerecordid><originalsourceid>FETCH-LOGICAL-c316t-4477241566b46708cfbd0c7f10c5d1fe8d8679ecd34c7936d4f6af81fb914b6d3</originalsourceid><addsrcrecordid>eNp1kE1LxDAQhoMouK7-AG8Bz9FMO01Sb0vxCwQR9BzaNOlmXZs1aQ_66-1awZOnmcPzvsM8hJwDvwTO5VWCvBAZ46AYR6EYHpAFLyUyyGRx-LMDkwrgmJyktOEcC8lxQZ6rdR1rM9jov-rBh54GR4e1pZ3tLbW9Ca3vO4rMhPF9Igd7XYUV3fquTpb6nlZha8dEXYjpLay33p-SI1dvkz37nUvyenvzUt2zx6e7h2r1yEwOYmCIUmYIhRANCsmVcU3LjXTATdGCs6pVQpbWtDkaWeaiRSdqp8A1JWAj2nxJLubeXQwfo02D3oQx9tNJnWV5DgWg4hMFM2ViSClap3fRT498auB6b07P5vRkTu_NaZwy2ZxJE9t3Nv41_x_6BvFBcF8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2233151480</pqid></control><display><type>article</type><title>Characterization of the gene encoding 4-coumarate:CoA ligase in Coleus forskohlii</title><source>Springer Nature - Complete Springer Journals</source><creator>Awasthi, Praveen ; Mahajan, Vidushi ; Jamwal, Vijay Lakshmi ; Chouhan, Rekha ; Kapoor, Nitika ; Bedi, Yashbir S. ; Gandhi, Sumit G.</creator><creatorcontrib>Awasthi, Praveen ; Mahajan, Vidushi ; Jamwal, Vijay Lakshmi ; Chouhan, Rekha ; Kapoor, Nitika ; Bedi, Yashbir S. ; Gandhi, Sumit G.</creatorcontrib><description>4-coumarate:coenzyme A ligase (4CL) converts 4-coumaric acid and its hydroxylated derivatives into the CoA thiol esters, directing carbon flux into various end-products of phenylpropanoid metabolism, such as flavonoids and lignins. In this study, full-length cDNA showing homology with plant
4CL
genes was cloned from
Coleus forskohlii
and was designated as
Cf4CL
(Accession No. KF643242).
Cf4CL
was found to contain an ORF of 1626 bps. The computational translation of
Cf4CL
encoded a protein of 541 amino acids. Theoretical isoelectric point and molecular weight of
Cf
4CL were calculated to be 5.55 and 58.65 kDa, respectively. Phylogenetic tree clustered
Cf4CL
with the class I 4CLs (involved in lignin biosynthesis). Spatial distribution of
Cf4CL
in different tissues and its expression in response to various stresses was carried out through qPCR. Abscisic acid (ABA) treatment strongly induced the expression of
Cf4CL
. Homology modeling and docking studies further ascertain the role of
Cf4CL
gene in lignin biosynthesis. In silico prediction suggested that
Cf4CL
may be post-transcriptionally regulated by microRNAs. Decreased expression of miR1886 in response to ABA treatment was associated with an increase in
Cf4CL
transcripts and lignin content, thus suggesting a possible role of miR1886 in regulating lignin biosynthesis in
C. forskohlii
.</description><identifier>ISSN: 0971-7811</identifier><identifier>EISSN: 0974-1275</identifier><identifier>DOI: 10.1007/s13562-018-0468-4</identifier><language>eng</language><publisher>New Delhi: Springer India</publisher><subject>Abscisic acid ; Amino acids ; Biomedical and Life Sciences ; Biosynthesis ; Cell Biology ; Coenzyme A ; Coleus forskohlii ; Computer applications ; Coumaric acid ; Docking ; Esters ; Flavonoids ; Homology ; Life Sciences ; Lignin ; Metabolism ; miRNA ; Molecular weight ; Open reading frames ; Original Article ; Phylogeny ; Plant Biochemistry ; Post-transcription ; Protein Science ; Receptors ; Spatial distribution ; Thioesters</subject><ispartof>Journal of plant biochemistry and biotechnology, 2019-06, Vol.28 (2), p.203-210</ispartof><rights>Society for Plant Biochemistry and Biotechnology 2018</rights><rights>Copyright Springer Nature B.V. 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-4477241566b46708cfbd0c7f10c5d1fe8d8679ecd34c7936d4f6af81fb914b6d3</citedby><cites>FETCH-LOGICAL-c316t-4477241566b46708cfbd0c7f10c5d1fe8d8679ecd34c7936d4f6af81fb914b6d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13562-018-0468-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13562-018-0468-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Awasthi, Praveen</creatorcontrib><creatorcontrib>Mahajan, Vidushi</creatorcontrib><creatorcontrib>Jamwal, Vijay Lakshmi</creatorcontrib><creatorcontrib>Chouhan, Rekha</creatorcontrib><creatorcontrib>Kapoor, Nitika</creatorcontrib><creatorcontrib>Bedi, Yashbir S.</creatorcontrib><creatorcontrib>Gandhi, Sumit G.</creatorcontrib><title>Characterization of the gene encoding 4-coumarate:CoA ligase in Coleus forskohlii</title><title>Journal of plant biochemistry and biotechnology</title><addtitle>J. Plant Biochem. Biotechnol</addtitle><description>4-coumarate:coenzyme A ligase (4CL) converts 4-coumaric acid and its hydroxylated derivatives into the CoA thiol esters, directing carbon flux into various end-products of phenylpropanoid metabolism, such as flavonoids and lignins. In this study, full-length cDNA showing homology with plant
4CL
genes was cloned from
Coleus forskohlii
and was designated as
Cf4CL
(Accession No. KF643242).
Cf4CL
was found to contain an ORF of 1626 bps. The computational translation of
Cf4CL
encoded a protein of 541 amino acids. Theoretical isoelectric point and molecular weight of
Cf
4CL were calculated to be 5.55 and 58.65 kDa, respectively. Phylogenetic tree clustered
Cf4CL
with the class I 4CLs (involved in lignin biosynthesis). Spatial distribution of
Cf4CL
in different tissues and its expression in response to various stresses was carried out through qPCR. Abscisic acid (ABA) treatment strongly induced the expression of
Cf4CL
. Homology modeling and docking studies further ascertain the role of
Cf4CL
gene in lignin biosynthesis. In silico prediction suggested that
Cf4CL
may be post-transcriptionally regulated by microRNAs. Decreased expression of miR1886 in response to ABA treatment was associated with an increase in
Cf4CL
transcripts and lignin content, thus suggesting a possible role of miR1886 in regulating lignin biosynthesis in
C. forskohlii
.</description><subject>Abscisic acid</subject><subject>Amino acids</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Cell Biology</subject><subject>Coenzyme A</subject><subject>Coleus forskohlii</subject><subject>Computer applications</subject><subject>Coumaric acid</subject><subject>Docking</subject><subject>Esters</subject><subject>Flavonoids</subject><subject>Homology</subject><subject>Life Sciences</subject><subject>Lignin</subject><subject>Metabolism</subject><subject>miRNA</subject><subject>Molecular weight</subject><subject>Open reading frames</subject><subject>Original Article</subject><subject>Phylogeny</subject><subject>Plant Biochemistry</subject><subject>Post-transcription</subject><subject>Protein Science</subject><subject>Receptors</subject><subject>Spatial distribution</subject><subject>Thioesters</subject><issn>0971-7811</issn><issn>0974-1275</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kE1LxDAQhoMouK7-AG8Bz9FMO01Sb0vxCwQR9BzaNOlmXZs1aQ_66-1awZOnmcPzvsM8hJwDvwTO5VWCvBAZ46AYR6EYHpAFLyUyyGRx-LMDkwrgmJyktOEcC8lxQZ6rdR1rM9jov-rBh54GR4e1pZ3tLbW9Ca3vO4rMhPF9Igd7XYUV3fquTpb6nlZha8dEXYjpLay33p-SI1dvkz37nUvyenvzUt2zx6e7h2r1yEwOYmCIUmYIhRANCsmVcU3LjXTATdGCs6pVQpbWtDkaWeaiRSdqp8A1JWAj2nxJLubeXQwfo02D3oQx9tNJnWV5DgWg4hMFM2ViSClap3fRT498auB6b07P5vRkTu_NaZwy2ZxJE9t3Nv41_x_6BvFBcF8</recordid><startdate>20190601</startdate><enddate>20190601</enddate><creator>Awasthi, Praveen</creator><creator>Mahajan, Vidushi</creator><creator>Jamwal, Vijay Lakshmi</creator><creator>Chouhan, Rekha</creator><creator>Kapoor, Nitika</creator><creator>Bedi, Yashbir S.</creator><creator>Gandhi, Sumit G.</creator><general>Springer India</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20190601</creationdate><title>Characterization of the gene encoding 4-coumarate:CoA ligase in Coleus forskohlii</title><author>Awasthi, Praveen ; Mahajan, Vidushi ; Jamwal, Vijay Lakshmi ; Chouhan, Rekha ; Kapoor, Nitika ; Bedi, Yashbir S. ; Gandhi, Sumit G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-4477241566b46708cfbd0c7f10c5d1fe8d8679ecd34c7936d4f6af81fb914b6d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Abscisic acid</topic><topic>Amino acids</topic><topic>Biomedical and Life Sciences</topic><topic>Biosynthesis</topic><topic>Cell Biology</topic><topic>Coenzyme A</topic><topic>Coleus forskohlii</topic><topic>Computer applications</topic><topic>Coumaric acid</topic><topic>Docking</topic><topic>Esters</topic><topic>Flavonoids</topic><topic>Homology</topic><topic>Life Sciences</topic><topic>Lignin</topic><topic>Metabolism</topic><topic>miRNA</topic><topic>Molecular weight</topic><topic>Open reading frames</topic><topic>Original Article</topic><topic>Phylogeny</topic><topic>Plant Biochemistry</topic><topic>Post-transcription</topic><topic>Protein Science</topic><topic>Receptors</topic><topic>Spatial distribution</topic><topic>Thioesters</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Awasthi, Praveen</creatorcontrib><creatorcontrib>Mahajan, Vidushi</creatorcontrib><creatorcontrib>Jamwal, Vijay Lakshmi</creatorcontrib><creatorcontrib>Chouhan, Rekha</creatorcontrib><creatorcontrib>Kapoor, Nitika</creatorcontrib><creatorcontrib>Bedi, Yashbir S.</creatorcontrib><creatorcontrib>Gandhi, Sumit G.</creatorcontrib><collection>CrossRef</collection><jtitle>Journal of plant biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Awasthi, Praveen</au><au>Mahajan, Vidushi</au><au>Jamwal, Vijay Lakshmi</au><au>Chouhan, Rekha</au><au>Kapoor, Nitika</au><au>Bedi, Yashbir S.</au><au>Gandhi, Sumit G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of the gene encoding 4-coumarate:CoA ligase in Coleus forskohlii</atitle><jtitle>Journal of plant biochemistry and biotechnology</jtitle><stitle>J. Plant Biochem. Biotechnol</stitle><date>2019-06-01</date><risdate>2019</risdate><volume>28</volume><issue>2</issue><spage>203</spage><epage>210</epage><pages>203-210</pages><issn>0971-7811</issn><eissn>0974-1275</eissn><abstract>4-coumarate:coenzyme A ligase (4CL) converts 4-coumaric acid and its hydroxylated derivatives into the CoA thiol esters, directing carbon flux into various end-products of phenylpropanoid metabolism, such as flavonoids and lignins. In this study, full-length cDNA showing homology with plant
4CL
genes was cloned from
Coleus forskohlii
and was designated as
Cf4CL
(Accession No. KF643242).
Cf4CL
was found to contain an ORF of 1626 bps. The computational translation of
Cf4CL
encoded a protein of 541 amino acids. Theoretical isoelectric point and molecular weight of
Cf
4CL were calculated to be 5.55 and 58.65 kDa, respectively. Phylogenetic tree clustered
Cf4CL
with the class I 4CLs (involved in lignin biosynthesis). Spatial distribution of
Cf4CL
in different tissues and its expression in response to various stresses was carried out through qPCR. Abscisic acid (ABA) treatment strongly induced the expression of
Cf4CL
. Homology modeling and docking studies further ascertain the role of
Cf4CL
gene in lignin biosynthesis. In silico prediction suggested that
Cf4CL
may be post-transcriptionally regulated by microRNAs. Decreased expression of miR1886 in response to ABA treatment was associated with an increase in
Cf4CL
transcripts and lignin content, thus suggesting a possible role of miR1886 in regulating lignin biosynthesis in
C. forskohlii
.</abstract><cop>New Delhi</cop><pub>Springer India</pub><doi>10.1007/s13562-018-0468-4</doi><tpages>8</tpages></addata></record> |
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| identifier | ISSN: 0971-7811 |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Abscisic acid Amino acids Biomedical and Life Sciences Biosynthesis Cell Biology Coenzyme A Coleus forskohlii Computer applications Coumaric acid Docking Esters Flavonoids Homology Life Sciences Lignin Metabolism miRNA Molecular weight Open reading frames Original Article Phylogeny Plant Biochemistry Post-transcription Protein Science Receptors Spatial distribution Thioesters |
| title | Characterization of the gene encoding 4-coumarate:CoA ligase in Coleus forskohlii |
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