Cytogenetic Stability of Young and Long-Term Embryogenic Cultures of Larix sibirica
We present the results of cytogenetic analysis of seven young and long-term cultured embryogenic cell lines (CLs) of Larix sibirica obtained by somatic embryogenesis. All CLs were found to be mixoploid. Three CLs (1, 2 and 6 years of cultivation) were the most stable cytogenetically with 91–96% cell...
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| Veröffentlicht in: | CYTOLOGIA 2018/09/25, Vol.83(3), pp.323-329 |
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| creator | Goryachkina, Olga V. Park, Maria E. Tretyakova, Iraida N. Badaeva, Ekaterina D. Muratova, Elena N. |
| description | We present the results of cytogenetic analysis of seven young and long-term cultured embryogenic cell lines (CLs) of Larix sibirica obtained by somatic embryogenesis. All CLs were found to be mixoploid. Three CLs (1, 2 and 6 years of cultivation) were the most stable cytogenetically with 91–96% cells having diploid chromosome number 2n=24, typical for the species. These CLs can be successfully used for the development of planting material and in clonal plantation forestry. At the same time, some embryogenic CLs were shown to possess various chromosome and genome mutations. We found two aneuploidy proliferating CLs (2n=25 and 28) capable of somatic embryo formation. One of the CLs analyzed in a current study was found to be cytogenetically unstable with chromosome numbers ranging from 24 to 35 and containing one-two micronuclei in the interphase cells. Fluorescent in situ hybridization (FISH) and morphometric analyses of karyotypes of these embryogenic CLs allow an assumption that the trisomy for one or several chromosomes is the most widespread type of genomic disturbances in embryogenic CLs of L. sibirica. |
| doi_str_mv | 10.1508/cytologia.83.323 |
| format | Article |
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All CLs were found to be mixoploid. Three CLs (1, 2 and 6 years of cultivation) were the most stable cytogenetically with 91–96% cells having diploid chromosome number 2n=24, typical for the species. These CLs can be successfully used for the development of planting material and in clonal plantation forestry. At the same time, some embryogenic CLs were shown to possess various chromosome and genome mutations. We found two aneuploidy proliferating CLs (2n=25 and 28) capable of somatic embryo formation. One of the CLs analyzed in a current study was found to be cytogenetically unstable with chromosome numbers ranging from 24 to 35 and containing one-two micronuclei in the interphase cells. Fluorescent in situ hybridization (FISH) and morphometric analyses of karyotypes of these embryogenic CLs allow an assumption that the trisomy for one or several chromosomes is the most widespread type of genomic disturbances in embryogenic CLs of L. sibirica.</description><identifier>ISSN: 0011-4545</identifier><identifier>EISSN: 1348-7019</identifier><identifier>DOI: 10.1508/cytologia.83.323</identifier><language>eng</language><publisher>Tokyo: Japan Mendel Society, International Society of Cytology</publisher><subject>Aneuploidy ; Cell lines ; Chromosome ; Chromosome number ; Chromosomes ; Cultivation ; Cytogenetics ; Embryogenic cell line ; Embryonic growth stage ; Embryos ; FISH ; Fluorescence ; Fluorescence in situ hybridization ; Forestry ; Genomes ; Karyotype ; Karyotypes ; Larix sibirica ; Micronuclei ; Mutation ; Plantations ; Somatic embryogenesis ; Trisomy</subject><ispartof>CYTOLOGIA, 2018/09/25, Vol.83(3), pp.323-329</ispartof><rights>2018 The Japan Mendel Society</rights><rights>Copyright Japan Science and Technology Agency 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c503t-30274ecc6ef77d47f7a3eb52470a7972738be5711ec3069e5058d216d69749c23</citedby><cites>FETCH-LOGICAL-c503t-30274ecc6ef77d47f7a3eb52470a7972738be5711ec3069e5058d216d69749c23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids></links><search><creatorcontrib>Goryachkina, Olga V.</creatorcontrib><creatorcontrib>Park, Maria E.</creatorcontrib><creatorcontrib>Tretyakova, Iraida N.</creatorcontrib><creatorcontrib>Badaeva, Ekaterina D.</creatorcontrib><creatorcontrib>Muratova, Elena N.</creatorcontrib><title>Cytogenetic Stability of Young and Long-Term Embryogenic Cultures of Larix sibirica</title><title>CYTOLOGIA</title><description>We present the results of cytogenetic analysis of seven young and long-term cultured embryogenic cell lines (CLs) of Larix sibirica obtained by somatic embryogenesis. All CLs were found to be mixoploid. Three CLs (1, 2 and 6 years of cultivation) were the most stable cytogenetically with 91–96% cells having diploid chromosome number 2n=24, typical for the species. These CLs can be successfully used for the development of planting material and in clonal plantation forestry. At the same time, some embryogenic CLs were shown to possess various chromosome and genome mutations. We found two aneuploidy proliferating CLs (2n=25 and 28) capable of somatic embryo formation. One of the CLs analyzed in a current study was found to be cytogenetically unstable with chromosome numbers ranging from 24 to 35 and containing one-two micronuclei in the interphase cells. Fluorescent in situ hybridization (FISH) and morphometric analyses of karyotypes of these embryogenic CLs allow an assumption that the trisomy for one or several chromosomes is the most widespread type of genomic disturbances in embryogenic CLs of L. sibirica.</description><subject>Aneuploidy</subject><subject>Cell lines</subject><subject>Chromosome</subject><subject>Chromosome number</subject><subject>Chromosomes</subject><subject>Cultivation</subject><subject>Cytogenetics</subject><subject>Embryogenic cell line</subject><subject>Embryonic growth stage</subject><subject>Embryos</subject><subject>FISH</subject><subject>Fluorescence</subject><subject>Fluorescence in situ hybridization</subject><subject>Forestry</subject><subject>Genomes</subject><subject>Karyotype</subject><subject>Karyotypes</subject><subject>Larix sibirica</subject><subject>Micronuclei</subject><subject>Mutation</subject><subject>Plantations</subject><subject>Somatic embryogenesis</subject><subject>Trisomy</subject><issn>0011-4545</issn><issn>1348-7019</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNpdkD1PwzAQhi0EElXpzhiJOeVsx7EzolC-FImhZWCyHMcJrtK4OIlE_j2OikBiuVue573Ti9A1hjVmIG71NLjWNVatBV1TQs_QAtNExBxwdo4WABjHCUvYJVr1vS0BSMoh42SBtnlQG9OZwepoO6jStnaYIldH727smkh1VVS4rol3xh-izaH004wHOB_bYfSmn9lCefsVhWTrrVZX6KJWbW9WP3uJ3h42u_wpLl4fn_O7ItYM6BBTIDwxWqem5rxKeM0VNSUjCQfFw3OcitIwjrHRFNLMMGCiIjit0ownmSZ0iW5OuUfvPkfTD3LvRt-Fk5IQijOSpCINFJwo7V3fe1PLo7cH5SeJQc7tyd_2pKAytBeUl5Oy7wfVmF9B-dBSa_4J87iXWEgAoPwP-lBemo5-A90-fyg</recordid><startdate>20180925</startdate><enddate>20180925</enddate><creator>Goryachkina, Olga V.</creator><creator>Park, Maria E.</creator><creator>Tretyakova, Iraida N.</creator><creator>Badaeva, Ekaterina D.</creator><creator>Muratova, Elena N.</creator><general>Japan Mendel Society, International Society of Cytology</general><general>Japan Science and Technology Agency</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20180925</creationdate><title>Cytogenetic Stability of Young and Long-Term Embryogenic Cultures of Larix sibirica</title><author>Goryachkina, Olga V. ; Park, Maria E. ; Tretyakova, Iraida N. ; Badaeva, Ekaterina D. ; Muratova, Elena N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c503t-30274ecc6ef77d47f7a3eb52470a7972738be5711ec3069e5058d216d69749c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Aneuploidy</topic><topic>Cell lines</topic><topic>Chromosome</topic><topic>Chromosome number</topic><topic>Chromosomes</topic><topic>Cultivation</topic><topic>Cytogenetics</topic><topic>Embryogenic cell line</topic><topic>Embryonic growth stage</topic><topic>Embryos</topic><topic>FISH</topic><topic>Fluorescence</topic><topic>Fluorescence in situ hybridization</topic><topic>Forestry</topic><topic>Genomes</topic><topic>Karyotype</topic><topic>Karyotypes</topic><topic>Larix sibirica</topic><topic>Micronuclei</topic><topic>Mutation</topic><topic>Plantations</topic><topic>Somatic embryogenesis</topic><topic>Trisomy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Goryachkina, Olga V.</creatorcontrib><creatorcontrib>Park, Maria E.</creatorcontrib><creatorcontrib>Tretyakova, Iraida N.</creatorcontrib><creatorcontrib>Badaeva, Ekaterina D.</creatorcontrib><creatorcontrib>Muratova, Elena N.</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>CYTOLOGIA</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Goryachkina, Olga V.</au><au>Park, Maria E.</au><au>Tretyakova, Iraida N.</au><au>Badaeva, Ekaterina D.</au><au>Muratova, Elena N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytogenetic Stability of Young and Long-Term Embryogenic Cultures of Larix sibirica</atitle><jtitle>CYTOLOGIA</jtitle><date>2018-09-25</date><risdate>2018</risdate><volume>83</volume><issue>3</issue><spage>323</spage><epage>329</epage><pages>323-329</pages><issn>0011-4545</issn><eissn>1348-7019</eissn><abstract>We present the results of cytogenetic analysis of seven young and long-term cultured embryogenic cell lines (CLs) of Larix sibirica obtained by somatic embryogenesis. All CLs were found to be mixoploid. Three CLs (1, 2 and 6 years of cultivation) were the most stable cytogenetically with 91–96% cells having diploid chromosome number 2n=24, typical for the species. These CLs can be successfully used for the development of planting material and in clonal plantation forestry. At the same time, some embryogenic CLs were shown to possess various chromosome and genome mutations. We found two aneuploidy proliferating CLs (2n=25 and 28) capable of somatic embryo formation. One of the CLs analyzed in a current study was found to be cytogenetically unstable with chromosome numbers ranging from 24 to 35 and containing one-two micronuclei in the interphase cells. Fluorescent in situ hybridization (FISH) and morphometric analyses of karyotypes of these embryogenic CLs allow an assumption that the trisomy for one or several chromosomes is the most widespread type of genomic disturbances in embryogenic CLs of L. sibirica.</abstract><cop>Tokyo</cop><pub>Japan Mendel Society, International Society of Cytology</pub><doi>10.1508/cytologia.83.323</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aneuploidy Cell lines Chromosome Chromosome number Chromosomes Cultivation Cytogenetics Embryogenic cell line Embryonic growth stage Embryos FISH Fluorescence Fluorescence in situ hybridization Forestry Genomes Karyotype Karyotypes Larix sibirica Micronuclei Mutation Plantations Somatic embryogenesis Trisomy |
| title | Cytogenetic Stability of Young and Long-Term Embryogenic Cultures of Larix sibirica |
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