Development of Amoxicillin Enzyme-Linked Immunosorbent Assay and Measurements of Tissue Amoxicillin Concentrations in a Pigeon Microdialysis Model

A sensitive ELISA was developed for the detection of amoxicillin (AMX) in serum, urine, and milk. The ELISA used an indirect competitive method produced by coating the plate with ovalbumin conjugated with AMX hapten. Antibodies against AMX-BSA were detected by a goat-antirabbit antibody conjugated w...

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Veröffentlicht in:	Poultry science 2008-03, Vol.87 (3), p.577-587
Hauptverfasser:	Yeh, L.-C, Lee, W.-M, Koh, B.-W, Chan, J.P, Liu, C.-H, Kao, J.-P, Chou, C.-C
Format:	Artikel
Sprache:	eng
Schlagworte:	amoxicillin Amoxicillin - blood Amoxicillin - pharmacokinetics Amoxicillin - urine Animals antibodies Area Under Curve blood serum calibration Columbidae - blood Columbidae - metabolism Columbidae - urine cross reaction Cross Reactions detection limit Dose-Response Relationship, Drug Drug Residues - analysis enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - veterinary inhibitory concentration 50 microdialysis Microdialysis - methods Microdialysis - veterinary Muscle, Skeletal - metabolism penicillins pharmacokinetics pigeons protein binding Sensitivity and Specificity skeletal muscle urine
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