Production of mRNA from the cry1Ac transgene differs among Bollgard® lines which correlates to the level of subsequent protein

Commercial cultivars of Bollgard cotton, Gossypium hirsutum L., differ in the amount of expressed Cry1Ac protein. However, the plant-mechanism for which this occurs is still unknown. Using quantitative real-time polymerase chain reaction (qPCR), we developed a method to determine if differences in t...

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Veröffentlicht in:	Transgenic research 2009-02, Vol.18 (1), p.143-149
Hauptverfasser:	Adamczyk, John J. Jr, Perera, Omaththage, Meredith, William R
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal Genetics and Genomics Bacillus thuringiensis subsp. berliner Bacterial Proteins - genetics Bacterial Proteins - metabolism Biomedical and Life Sciences Biomedical Engineering/Biotechnology Bollgard Brief Communication cotton crystal proteins cultivars delta-endotoxins Endotoxins - genetics Endotoxins - metabolism Enzyme-Linked Immunosorbent Assay gene expression Genetic Engineering Gossypium - genetics Gossypium - metabolism Gossypium hirsutum Hemolysin Proteins - genetics Hemolysin Proteins - metabolism insecticidal proteins Insecticides - metabolism Life Sciences messenger RNA Molecular Medicine Pest Control, Biological Plant Genetics and Genomics plant-incorporated protectants Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism polymerase chain reaction quantitative real-time polymerase chain reaction RNA, Messenger - genetics RNA, Messenger - metabolism transgenes Transgenes - physiology transgenic plants Transgenics
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