Effects of Hyperbaric Oxygen Therapy on Rat Nasal Mucosa
The objective of this study was to evaluate the histopathologic effects of hyperbaric oxygen (HBO) therapy on the rat nasal mucosa. Twelve adult Sprague-Dawley rats, each weighing 180-220 g, were used as experimental animals. The rats were divided into HBO (hyperbaric oxygen) and control group. The...
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| Veröffentlicht in: | Biotechnology, biotechnological equipment biotechnological equipment, 2012, Vol.26 (6), p.3394-3396 |
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| creator | Yorgancilar, Ediz Tunik, Selcuk Deveci, Engin Gun, Ramazan Bakir, Salih Kinis, Vefa Ayaz, Ercan Topcu, Ismail |
| description | The objective of this study was to evaluate the histopathologic effects of hyperbaric oxygen (HBO) therapy on the rat nasal mucosa. Twelve adult Sprague-Dawley rats, each weighing 180-220 g, were used as experimental animals. The rats were divided into HBO (hyperbaric oxygen) and control group. The rats in the HBO group (n = 6) were placed into a 20-liter HBO chamber (2.5 atmospheres absolute [ATA], 25-26°C with 100 % oxygen) for 90 min per day. The rats received hyperbaric oxygen over a period of 7 days. The rats in the control group (n = 6) were not given HBO. All animals were sacrificed at the end of the study, and nasal tissue samples were prepared. The sections were stained with Haematoxylin and Eosin (H-E), Periodic acid-Schiff (PAS) and Trichrome-Masson to observe the under a light microscope. Immunoreactivity of pseudostratified epithelial cells of the nasal mucosa was assessed with E-cadherin expression by immunohistochemical staining. There were significant differences in the average histopathological score between the groups exposed and non-exposed to HBO. In the HBO group, degenerative changes in epithelial cells were observed. The goblet cells showed expansion of their structure. Mononuclear lymphocyte infiltration, dilation of blood vessels, and hemorrhage were observed in considerable areas of connective tissue. In the immunohistochemical evaluation of E-cadherin expression, there were no significant differences between the two groups. |
| doi_str_mv | 10.5504/BBEQ.2012.0080 |
| format | Article |
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Twelve adult Sprague-Dawley rats, each weighing 180-220 g, were used as experimental animals. The rats were divided into HBO (hyperbaric oxygen) and control group. The rats in the HBO group (n = 6) were placed into a 20-liter HBO chamber (2.5 atmospheres absolute [ATA], 25-26°C with 100 % oxygen) for 90 min per day. The rats received hyperbaric oxygen over a period of 7 days. The rats in the control group (n = 6) were not given HBO. All animals were sacrificed at the end of the study, and nasal tissue samples were prepared. The sections were stained with Haematoxylin and Eosin (H-E), Periodic acid-Schiff (PAS) and Trichrome-Masson to observe the under a light microscope. Immunoreactivity of pseudostratified epithelial cells of the nasal mucosa was assessed with E-cadherin expression by immunohistochemical staining. There were significant differences in the average histopathological score between the groups exposed and non-exposed to HBO. In the HBO group, degenerative changes in epithelial cells were observed. The goblet cells showed expansion of their structure. Mononuclear lymphocyte infiltration, dilation of blood vessels, and hemorrhage were observed in considerable areas of connective tissue. In the immunohistochemical evaluation of E-cadherin expression, there were no significant differences between the two groups.</description><identifier>ISSN: 1310-2818</identifier><identifier>EISSN: 1314-3530</identifier><identifier>DOI: 10.5504/BBEQ.2012.0080</identifier><language>eng</language><publisher>Sofia: Taylor & Francis</publisher><subject>Animals ; Blood vessels ; Connective tissues ; E-cadherin ; Epithelial cells ; Evaluation ; Goblet cells ; Hemorrhage ; hyperbaric oxygen ; Hyperbaric oxygen therapy ; immunohistochemistry ; Immunoreactivity ; Lymphocytes ; Mucosa ; Mucous membrane ; nasal mucosa ; Oxygen ; rat ; Rodents</subject><ispartof>Biotechnology, biotechnological equipment, 2012, Vol.26 (6), p.3394-3396</ispartof><rights>2012 Taylor and Francis Group, LLC 2012</rights><rights>2012 Taylor and Francis Group, LLC</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c350t-9d2970bde7bf67f0e8f13df43999f414fad75d5713d0a0f99624c8ca0a600b863</citedby><cites>FETCH-LOGICAL-c350t-9d2970bde7bf67f0e8f13df43999f414fad75d5713d0a0f99624c8ca0a600b863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,4025,27928,27929,27930</link.rule.ids></links><search><creatorcontrib>Yorgancilar, Ediz</creatorcontrib><creatorcontrib>Tunik, Selcuk</creatorcontrib><creatorcontrib>Deveci, Engin</creatorcontrib><creatorcontrib>Gun, Ramazan</creatorcontrib><creatorcontrib>Bakir, Salih</creatorcontrib><creatorcontrib>Kinis, Vefa</creatorcontrib><creatorcontrib>Ayaz, Ercan</creatorcontrib><creatorcontrib>Topcu, Ismail</creatorcontrib><title>Effects of Hyperbaric Oxygen Therapy on Rat Nasal Mucosa</title><title>Biotechnology, biotechnological equipment</title><description>The objective of this study was to evaluate the histopathologic effects of hyperbaric oxygen (HBO) therapy on the rat nasal mucosa. Twelve adult Sprague-Dawley rats, each weighing 180-220 g, were used as experimental animals. The rats were divided into HBO (hyperbaric oxygen) and control group. The rats in the HBO group (n = 6) were placed into a 20-liter HBO chamber (2.5 atmospheres absolute [ATA], 25-26°C with 100 % oxygen) for 90 min per day. The rats received hyperbaric oxygen over a period of 7 days. The rats in the control group (n = 6) were not given HBO. All animals were sacrificed at the end of the study, and nasal tissue samples were prepared. The sections were stained with Haematoxylin and Eosin (H-E), Periodic acid-Schiff (PAS) and Trichrome-Masson to observe the under a light microscope. Immunoreactivity of pseudostratified epithelial cells of the nasal mucosa was assessed with E-cadherin expression by immunohistochemical staining. There were significant differences in the average histopathological score between the groups exposed and non-exposed to HBO. In the HBO group, degenerative changes in epithelial cells were observed. The goblet cells showed expansion of their structure. Mononuclear lymphocyte infiltration, dilation of blood vessels, and hemorrhage were observed in considerable areas of connective tissue. In the immunohistochemical evaluation of E-cadherin expression, there were no significant differences between the two groups.</description><subject>Animals</subject><subject>Blood vessels</subject><subject>Connective tissues</subject><subject>E-cadherin</subject><subject>Epithelial cells</subject><subject>Evaluation</subject><subject>Goblet cells</subject><subject>Hemorrhage</subject><subject>hyperbaric oxygen</subject><subject>Hyperbaric oxygen therapy</subject><subject>immunohistochemistry</subject><subject>Immunoreactivity</subject><subject>Lymphocytes</subject><subject>Mucosa</subject><subject>Mucous membrane</subject><subject>nasal mucosa</subject><subject>Oxygen</subject><subject>rat</subject><subject>Rodents</subject><issn>1310-2818</issn><issn>1314-3530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1kMFLwzAUh4MoOKdXzwUvXlpfkrZJjm5MJ0yHMs8hTRPt6JqZtGj_e1vnSfD0fjy-3-PxIXSJIckySG9ms8VzQgCTBIDDEZpgitOYZhSOfzLEhGN-is5C2AIwAMwmiC-sNboNkbPRst8bXyhf6Wj91b-ZJtq8G6_2feSa6EW10ZMKqo4eO-2COkcnVtXBXPzOKXq9W2zmy3i1vn-Y365iTTNoY1ESwaAoDStsziwYbjEtbUqFEDbFqVUly8qMDUtQYIXISaq5VqBygILndIquD3f33n10JrRyVwVt6lo1xnVBYiJwDpwxGNCrP-jWdb4ZvpOEEJFnWDAyUMmB0t6F4I2Ve1_tlO8lBjmKlKNIOYqUo8ihIA6FqrHO79Sn83UpW9XXzluvGl0FSf_pfgPgJnYo</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>Yorgancilar, Ediz</creator><creator>Tunik, Selcuk</creator><creator>Deveci, Engin</creator><creator>Gun, Ramazan</creator><creator>Bakir, Salih</creator><creator>Kinis, Vefa</creator><creator>Ayaz, Ercan</creator><creator>Topcu, Ismail</creator><general>Taylor & Francis</general><general>Taylor & Francis Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7ST</scope><scope>7XB</scope><scope>8FD</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>M2O</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>SOI</scope></search><sort><creationdate>2012</creationdate><title>Effects of Hyperbaric Oxygen Therapy on Rat Nasal Mucosa</title><author>Yorgancilar, Ediz ; Tunik, Selcuk ; Deveci, Engin ; Gun, Ramazan ; Bakir, Salih ; Kinis, Vefa ; Ayaz, Ercan ; Topcu, Ismail</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c350t-9d2970bde7bf67f0e8f13df43999f414fad75d5713d0a0f99624c8ca0a600b863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Blood vessels</topic><topic>Connective tissues</topic><topic>E-cadherin</topic><topic>Epithelial cells</topic><topic>Evaluation</topic><topic>Goblet cells</topic><topic>Hemorrhage</topic><topic>hyperbaric oxygen</topic><topic>Hyperbaric oxygen therapy</topic><topic>immunohistochemistry</topic><topic>Immunoreactivity</topic><topic>Lymphocytes</topic><topic>Mucosa</topic><topic>Mucous membrane</topic><topic>nasal mucosa</topic><topic>Oxygen</topic><topic>rat</topic><topic>Rodents</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yorgancilar, Ediz</creatorcontrib><creatorcontrib>Tunik, Selcuk</creatorcontrib><creatorcontrib>Deveci, Engin</creatorcontrib><creatorcontrib>Gun, Ramazan</creatorcontrib><creatorcontrib>Bakir, Salih</creatorcontrib><creatorcontrib>Kinis, Vefa</creatorcontrib><creatorcontrib>Ayaz, Ercan</creatorcontrib><creatorcontrib>Topcu, Ismail</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Technology Research Database</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Environment Abstracts</collection><jtitle>Biotechnology, biotechnological equipment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yorgancilar, Ediz</au><au>Tunik, Selcuk</au><au>Deveci, Engin</au><au>Gun, Ramazan</au><au>Bakir, Salih</au><au>Kinis, Vefa</au><au>Ayaz, Ercan</au><au>Topcu, Ismail</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Hyperbaric Oxygen Therapy on Rat Nasal Mucosa</atitle><jtitle>Biotechnology, biotechnological equipment</jtitle><date>2012</date><risdate>2012</risdate><volume>26</volume><issue>6</issue><spage>3394</spage><epage>3396</epage><pages>3394-3396</pages><issn>1310-2818</issn><eissn>1314-3530</eissn><abstract>The objective of this study was to evaluate the histopathologic effects of hyperbaric oxygen (HBO) therapy on the rat nasal mucosa. Twelve adult Sprague-Dawley rats, each weighing 180-220 g, were used as experimental animals. The rats were divided into HBO (hyperbaric oxygen) and control group. The rats in the HBO group (n = 6) were placed into a 20-liter HBO chamber (2.5 atmospheres absolute [ATA], 25-26°C with 100 % oxygen) for 90 min per day. The rats received hyperbaric oxygen over a period of 7 days. The rats in the control group (n = 6) were not given HBO. All animals were sacrificed at the end of the study, and nasal tissue samples were prepared. The sections were stained with Haematoxylin and Eosin (H-E), Periodic acid-Schiff (PAS) and Trichrome-Masson to observe the under a light microscope. Immunoreactivity of pseudostratified epithelial cells of the nasal mucosa was assessed with E-cadherin expression by immunohistochemical staining. There were significant differences in the average histopathological score between the groups exposed and non-exposed to HBO. In the HBO group, degenerative changes in epithelial cells were observed. The goblet cells showed expansion of their structure. Mononuclear lymphocyte infiltration, dilation of blood vessels, and hemorrhage were observed in considerable areas of connective tissue. In the immunohistochemical evaluation of E-cadherin expression, there were no significant differences between the two groups.</abstract><cop>Sofia</cop><pub>Taylor & Francis</pub><doi>10.5504/BBEQ.2012.0080</doi><tpages>3</tpages></addata></record> |
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| subjects | Animals Blood vessels Connective tissues E-cadherin Epithelial cells Evaluation Goblet cells Hemorrhage hyperbaric oxygen Hyperbaric oxygen therapy immunohistochemistry Immunoreactivity Lymphocytes Mucosa Mucous membrane nasal mucosa Oxygen rat Rodents |
| title | Effects of Hyperbaric Oxygen Therapy on Rat Nasal Mucosa |
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