Proteomic profile associated with cell death induced by androgens in Taenia crassiceps cysticerci: proposed interactome
Androgens have been shown to exert a cysticidal effect upon Taenia crassiceps, an experimental model of cysticercosis. To further inquire into this matter, the Taenia crassiceps model was used to evaluate the expression of several proteins after testosterone (T4) and dihydrotestosterone (DHT) in vit...
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| Veröffentlicht in: | Journal of helminthology 2019-09, Vol.93 (5), p.539-547 |
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| creator | Ambrosio, J.R. Palacios-Arreola, M.I. Ríos-Valencia, D.G. Reynoso-Ducoing, O. Nava-Castro, K.E. Ostoa-Saloma, P. Morales-Montor, J. |
| description | Androgens have been shown to exert a cysticidal effect upon Taenia crassiceps, an experimental model of cysticercosis. To further inquire into this matter, the Taenia crassiceps model was used to evaluate the expression of several proteins after testosterone (T4) and dihydrotestosterone (DHT) in vitro treatment. Under 2-D proteomic maps, parasite extracts were resolved into approximately 130 proteins distributed in a molecular weight range of 10–250 kDa and isoelectrical point range of 3–10. The resultant proteomic pattern was analysed, and significant changes were observed in response to T4 and DHT. Based on our experience with electrophoretic patterns and proteomic maps of cytoskeletal proteins, alteration in the expression of isoforms of actin, tubulin and paramyosin and of other proteins was assessed. Considering that androgens may exert their biological activity in taeniids through the non-specific progesterone receptor membrane component (PGRMC), we harnessed bioinformatics to propose the identity of androgen-regulated proteins and establish their hypothetical physiological role in the parasites. These analyses yield a possible explanation of how androgens exert their cysticidal effects through changes in the expression of proteins involved in cytoskeletal rearrangement, dynamic vesicular traffic and transduction of intracellular signals. |
| doi_str_mv | 10.1017/S0022149X18000706 |
| format | Article |
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To further inquire into this matter, the Taenia crassiceps model was used to evaluate the expression of several proteins after testosterone (T4) and dihydrotestosterone (DHT) in vitro treatment. Under 2-D proteomic maps, parasite extracts were resolved into approximately 130 proteins distributed in a molecular weight range of 10–250 kDa and isoelectrical point range of 3–10. The resultant proteomic pattern was analysed, and significant changes were observed in response to T4 and DHT. Based on our experience with electrophoretic patterns and proteomic maps of cytoskeletal proteins, alteration in the expression of isoforms of actin, tubulin and paramyosin and of other proteins was assessed. Considering that androgens may exert their biological activity in taeniids through the non-specific progesterone receptor membrane component (PGRMC), we harnessed bioinformatics to propose the identity of androgen-regulated proteins and establish their hypothetical physiological role in the parasites. These analyses yield a possible explanation of how androgens exert their cysticidal effects through changes in the expression of proteins involved in cytoskeletal rearrangement, dynamic vesicular traffic and transduction of intracellular signals.</description><identifier>ISSN: 0022-149X</identifier><identifier>EISSN: 1475-2697</identifier><identifier>DOI: 10.1017/S0022149X18000706</identifier><identifier>PMID: 30149822</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Actin ; Androgens ; Bioinformatics ; Biological activity ; Cell death ; Cysticercosis ; Cytoskeleton ; Dihydrotestosterone ; Ethanol ; Hormones ; Infections ; Isoforms ; Laboratory animals ; Metabolism ; Molecular weight ; Molecular weight distribution ; Parasites ; Pattern analysis ; Physiology ; Progesterone ; Proteins ; Receptors ; Research Paper ; Sex hormones ; Taenia crassiceps ; Testosterone ; Transduction ; Tubulin ; Worms</subject><ispartof>Journal of helminthology, 2019-09, Vol.93 (5), p.539-547</ispartof><rights>Copyright © Cambridge University Press 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c373t-f4675589ea2cc48203302a11cbcbd2b01ff2e36eee5e7e736b127f79ea0a63713</citedby><cites>FETCH-LOGICAL-c373t-f4675589ea2cc48203302a11cbcbd2b01ff2e36eee5e7e736b127f79ea0a63713</cites><orcidid>0000-0002-9461-610X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0022149X18000706/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,776,780,27901,27902,55603</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30149822$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ambrosio, J.R.</creatorcontrib><creatorcontrib>Palacios-Arreola, M.I.</creatorcontrib><creatorcontrib>Ríos-Valencia, D.G.</creatorcontrib><creatorcontrib>Reynoso-Ducoing, O.</creatorcontrib><creatorcontrib>Nava-Castro, K.E.</creatorcontrib><creatorcontrib>Ostoa-Saloma, P.</creatorcontrib><creatorcontrib>Morales-Montor, J.</creatorcontrib><title>Proteomic profile associated with cell death induced by androgens in Taenia crassiceps cysticerci: proposed interactome</title><title>Journal of helminthology</title><addtitle>J. Helminthol</addtitle><description>Androgens have been shown to exert a cysticidal effect upon Taenia crassiceps, an experimental model of cysticercosis. To further inquire into this matter, the Taenia crassiceps model was used to evaluate the expression of several proteins after testosterone (T4) and dihydrotestosterone (DHT) in vitro treatment. Under 2-D proteomic maps, parasite extracts were resolved into approximately 130 proteins distributed in a molecular weight range of 10–250 kDa and isoelectrical point range of 3–10. The resultant proteomic pattern was analysed, and significant changes were observed in response to T4 and DHT. Based on our experience with electrophoretic patterns and proteomic maps of cytoskeletal proteins, alteration in the expression of isoforms of actin, tubulin and paramyosin and of other proteins was assessed. Considering that androgens may exert their biological activity in taeniids through the non-specific progesterone receptor membrane component (PGRMC), we harnessed bioinformatics to propose the identity of androgen-regulated proteins and establish their hypothetical physiological role in the parasites. These analyses yield a possible explanation of how androgens exert their cysticidal effects through changes in the expression of proteins involved in cytoskeletal rearrangement, dynamic vesicular traffic and transduction of intracellular signals.</description><subject>Actin</subject><subject>Androgens</subject><subject>Bioinformatics</subject><subject>Biological activity</subject><subject>Cell death</subject><subject>Cysticercosis</subject><subject>Cytoskeleton</subject><subject>Dihydrotestosterone</subject><subject>Ethanol</subject><subject>Hormones</subject><subject>Infections</subject><subject>Isoforms</subject><subject>Laboratory animals</subject><subject>Metabolism</subject><subject>Molecular weight</subject><subject>Molecular weight distribution</subject><subject>Parasites</subject><subject>Pattern analysis</subject><subject>Physiology</subject><subject>Progesterone</subject><subject>Proteins</subject><subject>Receptors</subject><subject>Research Paper</subject><subject>Sex hormones</subject><subject>Taenia crassiceps</subject><subject>Testosterone</subject><subject>Transduction</subject><subject>Tubulin</subject><subject>Worms</subject><issn>0022-149X</issn><issn>1475-2697</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp1kN1LwzAUxYMobk7_AF8k4HM1H23T-SbDLxgoOMG3kqa3M6NtapIy-t-bsqkP4lMu557zu-EgdE7JFSVUXL8SwhiN5-80I4QIkh6gKY1FErF0Lg7RdFxH436CTpzbBA-nLDlGE06CmjE2RdsXazyYRivcWVPpGrB0zigtPZR4q_0HVlDXuAQZRt2WvQp6MWDZltasoXVBxCsJrZZY2ZDVCjqH1eB8mKzSNyO4My7EdOvBSuVNA6foqJK1g7P9O0Nv93erxWO0fH54WtwuI8UF91EVpyJJsjlIplScMcI5YZJSVaiiZAWhVcWApwCQgADB04IyUYngJzLlgvIZutxxwyc-e3A-35jetuFkzhgTIo6TdHTRnUtZ45yFKu-sbqQdckryser8T9Uhc7En90UD5U_iu9tg4HuobAqryzX83v4f-wVTsIqT</recordid><startdate>201909</startdate><enddate>201909</enddate><creator>Ambrosio, J.R.</creator><creator>Palacios-Arreola, M.I.</creator><creator>Ríos-Valencia, D.G.</creator><creator>Reynoso-Ducoing, O.</creator><creator>Nava-Castro, K.E.</creator><creator>Ostoa-Saloma, P.</creator><creator>Morales-Montor, J.</creator><general>Cambridge University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7SN</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H95</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.G</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><orcidid>https://orcid.org/0000-0002-9461-610X</orcidid></search><sort><creationdate>201909</creationdate><title>Proteomic profile associated with cell death induced by androgens in Taenia crassiceps cysticerci: proposed interactome</title><author>Ambrosio, J.R. ; Palacios-Arreola, M.I. ; Ríos-Valencia, D.G. ; Reynoso-Ducoing, O. ; Nava-Castro, K.E. ; Ostoa-Saloma, P. ; Morales-Montor, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c373t-f4675589ea2cc48203302a11cbcbd2b01ff2e36eee5e7e736b127f79ea0a63713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Actin</topic><topic>Androgens</topic><topic>Bioinformatics</topic><topic>Biological activity</topic><topic>Cell death</topic><topic>Cysticercosis</topic><topic>Cytoskeleton</topic><topic>Dihydrotestosterone</topic><topic>Ethanol</topic><topic>Hormones</topic><topic>Infections</topic><topic>Isoforms</topic><topic>Laboratory animals</topic><topic>Metabolism</topic><topic>Molecular weight</topic><topic>Molecular weight distribution</topic><topic>Parasites</topic><topic>Pattern analysis</topic><topic>Physiology</topic><topic>Progesterone</topic><topic>Proteins</topic><topic>Receptors</topic><topic>Research Paper</topic><topic>Sex hormones</topic><topic>Taenia crassiceps</topic><topic>Testosterone</topic><topic>Transduction</topic><topic>Tubulin</topic><topic>Worms</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ambrosio, J.R.</creatorcontrib><creatorcontrib>Palacios-Arreola, M.I.</creatorcontrib><creatorcontrib>Ríos-Valencia, D.G.</creatorcontrib><creatorcontrib>Reynoso-Ducoing, O.</creatorcontrib><creatorcontrib>Nava-Castro, K.E.</creatorcontrib><creatorcontrib>Ostoa-Saloma, P.</creatorcontrib><creatorcontrib>Morales-Montor, J.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Ecology Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Agriculture & Environmental Science Database</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Journal of helminthology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ambrosio, J.R.</au><au>Palacios-Arreola, M.I.</au><au>Ríos-Valencia, D.G.</au><au>Reynoso-Ducoing, O.</au><au>Nava-Castro, K.E.</au><au>Ostoa-Saloma, P.</au><au>Morales-Montor, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proteomic profile associated with cell death induced by androgens in Taenia crassiceps cysticerci: proposed interactome</atitle><jtitle>Journal of helminthology</jtitle><addtitle>J. Helminthol</addtitle><date>2019-09</date><risdate>2019</risdate><volume>93</volume><issue>5</issue><spage>539</spage><epage>547</epage><pages>539-547</pages><issn>0022-149X</issn><eissn>1475-2697</eissn><abstract>Androgens have been shown to exert a cysticidal effect upon Taenia crassiceps, an experimental model of cysticercosis. To further inquire into this matter, the Taenia crassiceps model was used to evaluate the expression of several proteins after testosterone (T4) and dihydrotestosterone (DHT) in vitro treatment. Under 2-D proteomic maps, parasite extracts were resolved into approximately 130 proteins distributed in a molecular weight range of 10–250 kDa and isoelectrical point range of 3–10. The resultant proteomic pattern was analysed, and significant changes were observed in response to T4 and DHT. Based on our experience with electrophoretic patterns and proteomic maps of cytoskeletal proteins, alteration in the expression of isoforms of actin, tubulin and paramyosin and of other proteins was assessed. Considering that androgens may exert their biological activity in taeniids through the non-specific progesterone receptor membrane component (PGRMC), we harnessed bioinformatics to propose the identity of androgen-regulated proteins and establish their hypothetical physiological role in the parasites. These analyses yield a possible explanation of how androgens exert their cysticidal effects through changes in the expression of proteins involved in cytoskeletal rearrangement, dynamic vesicular traffic and transduction of intracellular signals.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>30149822</pmid><doi>10.1017/S0022149X18000706</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-9461-610X</orcidid></addata></record> |
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| ispartof | Journal of helminthology, 2019-09, Vol.93 (5), p.539-547 |
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| subjects | Actin Androgens Bioinformatics Biological activity Cell death Cysticercosis Cytoskeleton Dihydrotestosterone Ethanol Hormones Infections Isoforms Laboratory animals Metabolism Molecular weight Molecular weight distribution Parasites Pattern analysis Physiology Progesterone Proteins Receptors Research Paper Sex hormones Taenia crassiceps Testosterone Transduction Tubulin Worms |
| title | Proteomic profile associated with cell death induced by androgens in Taenia crassiceps cysticerci: proposed interactome |
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