Photo-mediated gene activation using caged RNA/DNA in zebrafish embryos

We report a new and simple technique for photo-mediated temporal and spatial control of gene activation in zebrafish embryos as an alternative to the gene 'knockdown' approach using antisense, morpholino-modified oligonucleotides (morpholinos). The synthetic compound 6-bromo-4-diazomethyl-...

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Veröffentlicht in:	Nature genetics 2001-08, Vol.28 (4), p.317-325
Hauptverfasser:	Ando, Hideki, Furuta, Toshiaki, Tsien, Roger Y., Okamoto, Hitoshi
Format:	Artikel
Sprache:	eng
Schlagworte:	Acids Agriculture Animal Genetics and Genomics Animals Azo Compounds - chemistry Azo Compounds - pharmacology Biological and medical sciences Biomedical and Life Sciences Biomedicine Brain research Cancer Research Coumarins - chemistry Coumarins - pharmacology DNA DNA - administration & dosage DNA - chemistry DNA - radiation effects Embryo, Nonmammalian - drug effects Embryo, Nonmammalian - metabolism Embryo, Nonmammalian - pathology Embryos Eye Abnormalities - chemically induced Eye Abnormalities - embryology Eye Abnormalities - pathology Fluorescence Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation, Developmental - drug effects Gene Expression Regulation, Developmental - radiation effects Gene Function Genes. Genome Genetics Green Fluorescent Proteins Homeodomain Proteins - biosynthesis Homeodomain Proteins - genetics Homeodomain Proteins - pharmacology Human Genetics Light Luminescent Proteins - biosynthesis Luminescent Proteins - genetics Microinjections Molecular and cellular biology Molecular genetics Nerve Tissue Proteins - biosynthesis Nerve Tissue Proteins - genetics Nerve Tissue Proteins - pharmacology Nervous System Malformations - chemically induced Nervous System Malformations - embryology Nervous System Malformations - pathology new-technology Oryzias - genetics Phosphates Photolysis Physiological aspects Prosencephalon - abnormalities Prosencephalon - drug effects Prosencephalon - metabolism Protein Biosynthesis - radiation effects Publishing RNA RNA Stability - drug effects RNA, Messenger - administration & dosage RNA, Messenger - chemistry RNA, Messenger - radiation effects Science Sugar Transcriptional Activation Ultraviolet radiation Ultraviolet Rays Zebrafish
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