Effects of ethanol on human periodontal ligament fibroblasts subjected to static compressive force
Consumption of toxic substances such as alcohol is widespread in the general population and thus also in patients receiving orthodontic treatment. Since human periodontal ligament (hPDL) fibroblasts play a key role in orthodontic tooth movement (OTM) by expressing cytokines and chemokines, we wanted...
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| creator | Schröder, Agnes Küchler, Erika Calvano Omori, Marjorie Spanier, Gerrit Proff, Peter Kirschneck, Christian |
| description | Consumption of toxic substances such as alcohol is widespread in the general population and thus also in patients receiving orthodontic treatment. Since human periodontal ligament (hPDL) fibroblasts play a key role in orthodontic tooth movement (OTM) by expressing cytokines and chemokines, we wanted to clarify whether ethanol modulates the physiological activity and expression pattern of hPDL fibroblasts during static compressive force application. We pre-incubated hPDL fibroblasts for 24 h with different ethanol concentrations, corresponding to casual (0.041% blood alcohol concentration [BAC], % by volume) and excessive (0.179%) alcohol consumption. At each ethanol concentration, we incubated the cells for another 48 h with and without an additional physiological compressive force of 2 g/cm2 occurring during orthodontic tooth movement in compression areas of the periodontal ligament. Thereafter, we analyzed expression and secretion of genes and proteins involved in OTM regulation by RT-qPCR and ELISA. We also performed co-culture experiments to observe hPDL-fibroblast-mediated osteoclastogenesis. We observed no effects of ethanol on cytotoxicity or cell viability of hPDL fibroblasts in the applied concentrations. Ethanol showed an enhancing effect on angiogenesis and activity of alkaline phosphatase. Simultaneously, ethanol reduced the induction of IL-6 and increased prostaglandin E2 synthesis as well as hPDL-fibroblast-mediated osteoclastogenesis without affecting the RANK-L/OPG-system. hPDL fibroblasts thus seem to be a cell type quite resistant to ethanol, as no cytotoxic effects or influence on cell viability were detected. High ethanol concentrations, however, seem to promote bone formation and angiogenesis. Ethanol at 0.179% also enhanced hPDL-induced osteoclastogenesis, indicating increased bone resorption and thus tooth movement velocity to be expected during orthodontic therapy.
•hPDL fibroblasts play a key mediating role in orthodontic tooth movement.•0.041% and 0.179% of ethanol did not affect hPDL fibroblast viability or vitality.•Ethanol may increase alveolar angiogenesis, bone formation, and collagen synthesis.•Ethanol increased hPDL-fibroblast-mediated osteoclastogenesis.•Ethanol may increase tooth movement and bone loss during orthodontic treatment. |
| doi_str_mv | 10.1016/j.alcohol.2018.10.004 |
| format | Article |
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•hPDL fibroblasts play a key mediating role in orthodontic tooth movement.•0.041% and 0.179% of ethanol did not affect hPDL fibroblast viability or vitality.•Ethanol may increase alveolar angiogenesis, bone formation, and collagen synthesis.•Ethanol increased hPDL-fibroblast-mediated osteoclastogenesis.•Ethanol may increase tooth movement and bone loss during orthodontic treatment.</description><identifier>ISSN: 0741-8329</identifier><identifier>EISSN: 1873-6823</identifier><identifier>DOI: 10.1016/j.alcohol.2018.10.004</identifier><identifier>PMID: 30336201</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adolescent ; Adult ; Alcohol ; Alcohol use ; Alcoholic beverages ; Alkaline phosphatase ; Angiogenesis ; Apoptosis ; Apoptosis - drug effects ; Apoptosis - physiology ; Blood levels ; Bone growth ; Bone resorption ; Cell culture ; Cell viability ; Cells, Cultured ; Chemokines ; Compression ; Compressive force ; Compressive Strength - drug effects ; Compressive Strength - physiology ; Cytokines ; Cytotoxicity ; Enzyme-linked immunosorbent assay ; Ethanol ; Ethanol - administration & dosage ; Ethics ; Female ; Fibroblasts ; Fibroblasts - drug effects ; Fibroblasts - physiology ; Genes ; Humans ; Interleukin 6 ; Ligaments ; Male ; Orthodontics ; Osteoclastogenesis ; Osteogenesis ; Osteoprotegerin ; PDL ; Periodontal ligament ; Periodontal Ligament - cytology ; Periodontal Ligament - drug effects ; Periodontal Ligament - physiology ; Periodontal ligament fibroblast ; Physiology ; Prostaglandin E2 ; Teeth ; Toxic substances ; TRANCE protein ; Tumor necrosis factor-TNF ; Young Adult</subject><ispartof>Alcohol (Fayetteville, N.Y.), 2019-06, Vol.77, p.59-70</ispartof><rights>2018 Elsevier Inc.</rights><rights>Copyright © 2018 Elsevier Inc. All rights reserved.</rights><rights>2018. Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c393t-3b51e32d52c6b0cc50f8ef9ef702ac48ae82a4632ee6ea80ec2d07b9da250893</citedby><cites>FETCH-LOGICAL-c393t-3b51e32d52c6b0cc50f8ef9ef702ac48ae82a4632ee6ea80ec2d07b9da250893</cites><orcidid>0000-0001-9473-8724</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/2225710984?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993,64383,64387,72239</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30336201$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schröder, Agnes</creatorcontrib><creatorcontrib>Küchler, Erika Calvano</creatorcontrib><creatorcontrib>Omori, Marjorie</creatorcontrib><creatorcontrib>Spanier, Gerrit</creatorcontrib><creatorcontrib>Proff, Peter</creatorcontrib><creatorcontrib>Kirschneck, Christian</creatorcontrib><title>Effects of ethanol on human periodontal ligament fibroblasts subjected to static compressive force</title><title>Alcohol (Fayetteville, N.Y.)</title><addtitle>Alcohol</addtitle><description>Consumption of toxic substances such as alcohol is widespread in the general population and thus also in patients receiving orthodontic treatment. Since human periodontal ligament (hPDL) fibroblasts play a key role in orthodontic tooth movement (OTM) by expressing cytokines and chemokines, we wanted to clarify whether ethanol modulates the physiological activity and expression pattern of hPDL fibroblasts during static compressive force application. We pre-incubated hPDL fibroblasts for 24 h with different ethanol concentrations, corresponding to casual (0.041% blood alcohol concentration [BAC], % by volume) and excessive (0.179%) alcohol consumption. At each ethanol concentration, we incubated the cells for another 48 h with and without an additional physiological compressive force of 2 g/cm2 occurring during orthodontic tooth movement in compression areas of the periodontal ligament. Thereafter, we analyzed expression and secretion of genes and proteins involved in OTM regulation by RT-qPCR and ELISA. We also performed co-culture experiments to observe hPDL-fibroblast-mediated osteoclastogenesis. We observed no effects of ethanol on cytotoxicity or cell viability of hPDL fibroblasts in the applied concentrations. Ethanol showed an enhancing effect on angiogenesis and activity of alkaline phosphatase. Simultaneously, ethanol reduced the induction of IL-6 and increased prostaglandin E2 synthesis as well as hPDL-fibroblast-mediated osteoclastogenesis without affecting the RANK-L/OPG-system. hPDL fibroblasts thus seem to be a cell type quite resistant to ethanol, as no cytotoxic effects or influence on cell viability were detected. High ethanol concentrations, however, seem to promote bone formation and angiogenesis. Ethanol at 0.179% also enhanced hPDL-induced osteoclastogenesis, indicating increased bone resorption and thus tooth movement velocity to be expected during orthodontic therapy.
•hPDL fibroblasts play a key mediating role in orthodontic tooth movement.•0.041% and 0.179% of ethanol did not affect hPDL fibroblast viability or vitality.•Ethanol may increase alveolar angiogenesis, bone formation, and collagen synthesis.•Ethanol increased hPDL-fibroblast-mediated osteoclastogenesis.•Ethanol may increase tooth movement and bone loss during orthodontic treatment.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Alcohol</subject><subject>Alcohol use</subject><subject>Alcoholic beverages</subject><subject>Alkaline phosphatase</subject><subject>Angiogenesis</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Apoptosis - physiology</subject><subject>Blood levels</subject><subject>Bone growth</subject><subject>Bone resorption</subject><subject>Cell culture</subject><subject>Cell viability</subject><subject>Cells, Cultured</subject><subject>Chemokines</subject><subject>Compression</subject><subject>Compressive force</subject><subject>Compressive Strength - drug effects</subject><subject>Compressive Strength - physiology</subject><subject>Cytokines</subject><subject>Cytotoxicity</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Ethanol</subject><subject>Ethanol - administration & dosage</subject><subject>Ethics</subject><subject>Female</subject><subject>Fibroblasts</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - physiology</subject><subject>Genes</subject><subject>Humans</subject><subject>Interleukin 6</subject><subject>Ligaments</subject><subject>Male</subject><subject>Orthodontics</subject><subject>Osteoclastogenesis</subject><subject>Osteogenesis</subject><subject>Osteoprotegerin</subject><subject>PDL</subject><subject>Periodontal ligament</subject><subject>Periodontal Ligament - cytology</subject><subject>Periodontal Ligament - drug effects</subject><subject>Periodontal Ligament - physiology</subject><subject>Periodontal ligament fibroblast</subject><subject>Physiology</subject><subject>Prostaglandin E2</subject><subject>Teeth</subject><subject>Toxic substances</subject><subject>TRANCE protein</subject><subject>Tumor necrosis factor-TNF</subject><subject>Young Adult</subject><issn>0741-8329</issn><issn>1873-6823</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkEtLLDEQRoNc0fHxE7wE7rrHStKP9EpEfIHgxn1IpytOmu7O3CQt-O_NMKNbVwXFd76iDiFXDNYMWH09rPVo_MaPaw5M5t0aoDwiKyYbUdSSiz9kBU3JCil4e0rOYhwAoGma9oScChCiztyKdPfWokmReksxbfTsR-pnulkmPdMtBud7Pyc90tG96wnnRK3rgu9GHTMUl27INPY0eRqTTs5Q46dtwBjdB1Lrg8ELcmz1GPHyMM_J28P9291T8fL6-Hx3-1IY0YpUiK5iKHhfcVN3YEwFVqJt0TbAtSmlRsl1WQuOWKOWgIb30HRtr3kFshXn5N--dhv8_wVjUoNfwpwvKs551TBoZZlT1T5lgo8xoFXb4CYdPhUDtROrBnUQq3Zid-ssNnN_D-1LN2H_Q32bzIGbfQDzix8Og4rG4WywdyErUr13v5z4Ak79jg0</recordid><startdate>201906</startdate><enddate>201906</enddate><creator>Schröder, Agnes</creator><creator>Küchler, Erika Calvano</creator><creator>Omori, Marjorie</creator><creator>Spanier, Gerrit</creator><creator>Proff, Peter</creator><creator>Kirschneck, Christian</creator><general>Elsevier Inc</general><general>Elsevier Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>0-V</scope><scope>3V.</scope><scope>7QG</scope><scope>7RV</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8AM</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ALSLI</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGRYB</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K7.</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0O</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><orcidid>https://orcid.org/0000-0001-9473-8724</orcidid></search><sort><creationdate>201906</creationdate><title>Effects of ethanol on human periodontal ligament fibroblasts subjected to static compressive force</title><author>Schröder, Agnes ; Küchler, Erika Calvano ; Omori, Marjorie ; Spanier, Gerrit ; Proff, Peter ; Kirschneck, Christian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c393t-3b51e32d52c6b0cc50f8ef9ef702ac48ae82a4632ee6ea80ec2d07b9da250893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Alcohol</topic><topic>Alcohol use</topic><topic>Alcoholic beverages</topic><topic>Alkaline phosphatase</topic><topic>Angiogenesis</topic><topic>Apoptosis</topic><topic>Apoptosis - 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Since human periodontal ligament (hPDL) fibroblasts play a key role in orthodontic tooth movement (OTM) by expressing cytokines and chemokines, we wanted to clarify whether ethanol modulates the physiological activity and expression pattern of hPDL fibroblasts during static compressive force application. We pre-incubated hPDL fibroblasts for 24 h with different ethanol concentrations, corresponding to casual (0.041% blood alcohol concentration [BAC], % by volume) and excessive (0.179%) alcohol consumption. At each ethanol concentration, we incubated the cells for another 48 h with and without an additional physiological compressive force of 2 g/cm2 occurring during orthodontic tooth movement in compression areas of the periodontal ligament. Thereafter, we analyzed expression and secretion of genes and proteins involved in OTM regulation by RT-qPCR and ELISA. We also performed co-culture experiments to observe hPDL-fibroblast-mediated osteoclastogenesis. We observed no effects of ethanol on cytotoxicity or cell viability of hPDL fibroblasts in the applied concentrations. Ethanol showed an enhancing effect on angiogenesis and activity of alkaline phosphatase. Simultaneously, ethanol reduced the induction of IL-6 and increased prostaglandin E2 synthesis as well as hPDL-fibroblast-mediated osteoclastogenesis without affecting the RANK-L/OPG-system. hPDL fibroblasts thus seem to be a cell type quite resistant to ethanol, as no cytotoxic effects or influence on cell viability were detected. High ethanol concentrations, however, seem to promote bone formation and angiogenesis. Ethanol at 0.179% also enhanced hPDL-induced osteoclastogenesis, indicating increased bone resorption and thus tooth movement velocity to be expected during orthodontic therapy.
•hPDL fibroblasts play a key mediating role in orthodontic tooth movement.•0.041% and 0.179% of ethanol did not affect hPDL fibroblast viability or vitality.•Ethanol may increase alveolar angiogenesis, bone formation, and collagen synthesis.•Ethanol increased hPDL-fibroblast-mediated osteoclastogenesis.•Ethanol may increase tooth movement and bone loss during orthodontic treatment.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>30336201</pmid><doi>10.1016/j.alcohol.2018.10.004</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-9473-8724</orcidid></addata></record> |
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| subjects | Adolescent Adult Alcohol Alcohol use Alcoholic beverages Alkaline phosphatase Angiogenesis Apoptosis Apoptosis - drug effects Apoptosis - physiology Blood levels Bone growth Bone resorption Cell culture Cell viability Cells, Cultured Chemokines Compression Compressive force Compressive Strength - drug effects Compressive Strength - physiology Cytokines Cytotoxicity Enzyme-linked immunosorbent assay Ethanol Ethanol - administration & dosage Ethics Female Fibroblasts Fibroblasts - drug effects Fibroblasts - physiology Genes Humans Interleukin 6 Ligaments Male Orthodontics Osteoclastogenesis Osteogenesis Osteoprotegerin PDL Periodontal ligament Periodontal Ligament - cytology Periodontal Ligament - drug effects Periodontal Ligament - physiology Periodontal ligament fibroblast Physiology Prostaglandin E2 Teeth Toxic substances TRANCE protein Tumor necrosis factor-TNF Young Adult |
| title | Effects of ethanol on human periodontal ligament fibroblasts subjected to static compressive force |
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