Cardiac troponin T alterations in myocardium and serum of rats after stressful, prolonged intense exercise
1 Division of Kinesiology, School of Kinesiology and Leisure Studies, University of Minnesota, Minneapolis 55455; 2 Department of Laboratory Medicine and Pathology, School of Medicine, University of Minnesota, Hennepin County Medical Center, Minneapolis 55404; and 3 Jesse E. Edwards Registry of C...
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| Veröffentlicht in: | Journal of applied physiology (1985) 2000-05, Vol.88 (5), p.1749-1755 |
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| container_title | Journal of applied physiology (1985) |
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| creator | Chen, Yingjie Serfass, Robert C Mackey-Bojack, Shannon M Kelly, Karen L Titus, Jack L Apple, Fred S |
| description | 1 Division of Kinesiology, School of
Kinesiology and Leisure Studies, University of Minnesota, Minneapolis
55455; 2 Department of Laboratory Medicine and
Pathology, School of Medicine, University of Minnesota, Hennepin County
Medical Center, Minneapolis 55404; and
3 Jesse E. Edwards Registry of Cardiovascular
Disease, St. Paul, Minnesota 55102
The goal
of this study was to determine whether the stress of forced exercise
would result in injury to the myocardium. Male rats with 8% of body
weight attached to the tail were forced to swim 3.5 h (3.5S), forced to
swim 5 h (5S), or pretrained for 8 days and then forced to swim 5 h
(T5S). Rats were killed immediately after they swam (0 h PS) and at 3 h
(3 h PS), 24 h (24 h PS), and 48 h after they swam (48 h PS). Tissue
homogenates of the left ventricle were analyzed by Western blot
analysis for cardiac troponin T (cTnT). Serum cTnT was quantified by
immunoassay. Results indicated that, in the 3.5S, 5S, and T5S groups,
serum cTnT was significantly ( P |
| doi_str_mv | 10.1152/jappl.2000.88.5.1749 |
| format | Article |
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Kinesiology and Leisure Studies, University of Minnesota, Minneapolis
55455; 2 Department of Laboratory Medicine and
Pathology, School of Medicine, University of Minnesota, Hennepin County
Medical Center, Minneapolis 55404; and
3 Jesse E. Edwards Registry of Cardiovascular
Disease, St. Paul, Minnesota 55102
The goal
of this study was to determine whether the stress of forced exercise
would result in injury to the myocardium. Male rats with 8% of body
weight attached to the tail were forced to swim 3.5 h (3.5S), forced to
swim 5 h (5S), or pretrained for 8 days and then forced to swim 5 h
(T5S). Rats were killed immediately after they swam (0 h PS) and at 3 h
(3 h PS), 24 h (24 h PS), and 48 h after they swam (48 h PS). Tissue
homogenates of the left ventricle were analyzed by Western blot
analysis for cardiac troponin T (cTnT). Serum cTnT was quantified by
immunoassay. Results indicated that, in the 3.5S, 5S, and T5S groups,
serum cTnT was significantly ( P < 0.01) increased at 0 and 3 h PS. The 5S group demonstrated a greater increase in serum cTnT than
the 3.5S group ( P < 0.01) and the T5S group ( P < 0.01) at 0 h PS. Western blot analysis indicated significant decreases
( P < 0.01) in myocardial cTnT in the 5S group only at 0 h PS
( P < 0.01) and 3 h PS ( P < 0.05). Histological
evidence of localized myocyte damage demonstrated by interstitial
inflammatory infiltrates consisting of neutrophils, lymphocytes, and
histiocytes, as well as vesicular nuclei-enlarged chromatin patterns,
was observed in left ventricle specimens from the 5S group at 24 and 48 h PS. Our findings demonstrate that stressful, forced exercise induces
alterations in myocardial cTnT and that training before exercise
attenuates the exercise-induced heart damage.
myocardial damage; exercise stress; histology]]></description><identifier>ISSN: 8750-7587</identifier><identifier>EISSN: 1522-1601</identifier><identifier>DOI: 10.1152/jappl.2000.88.5.1749</identifier><identifier>PMID: 10797139</identifier><identifier>CODEN: JAPHEV</identifier><language>eng</language><publisher>Bethesda, MD: Am Physiological Soc</publisher><subject>Anatomy & physiology ; Animals ; Biological and medical sciences ; Exercise ; Fundamental and applied biological sciences. Psychology ; Health risk assessment ; Heart ; Heart attacks ; Male ; Motor Activity - physiology ; Myocardium - metabolism ; Physical Endurance ; Rats ; Rats, Sprague-Dawley ; Rodents ; Stress, Physiological - blood ; Time Factors ; Troponin T - blood ; Troponin T - metabolism ; Vertebrates: cardiovascular system</subject><ispartof>Journal of applied physiology (1985), 2000-05, Vol.88 (5), p.1749-1755</ispartof><rights>2000 INIST-CNRS</rights><rights>Copyright American Physiological Society May 2000</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-6701616de72e6efd4bb29897022a490fbf0d571330b3b9bcc7947292c71c8f023</citedby><cites>FETCH-LOGICAL-c483t-6701616de72e6efd4bb29897022a490fbf0d571330b3b9bcc7947292c71c8f023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3026,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1427713$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10797139$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Yingjie</creatorcontrib><creatorcontrib>Serfass, Robert C</creatorcontrib><creatorcontrib>Mackey-Bojack, Shannon M</creatorcontrib><creatorcontrib>Kelly, Karen L</creatorcontrib><creatorcontrib>Titus, Jack L</creatorcontrib><creatorcontrib>Apple, Fred S</creatorcontrib><title>Cardiac troponin T alterations in myocardium and serum of rats after stressful, prolonged intense exercise</title><title>Journal of applied physiology (1985)</title><addtitle>J Appl Physiol (1985)</addtitle><description><![CDATA[1 Division of Kinesiology, School of
Kinesiology and Leisure Studies, University of Minnesota, Minneapolis
55455; 2 Department of Laboratory Medicine and
Pathology, School of Medicine, University of Minnesota, Hennepin County
Medical Center, Minneapolis 55404; and
3 Jesse E. Edwards Registry of Cardiovascular
Disease, St. Paul, Minnesota 55102
The goal
of this study was to determine whether the stress of forced exercise
would result in injury to the myocardium. Male rats with 8% of body
weight attached to the tail were forced to swim 3.5 h (3.5S), forced to
swim 5 h (5S), or pretrained for 8 days and then forced to swim 5 h
(T5S). Rats were killed immediately after they swam (0 h PS) and at 3 h
(3 h PS), 24 h (24 h PS), and 48 h after they swam (48 h PS). Tissue
homogenates of the left ventricle were analyzed by Western blot
analysis for cardiac troponin T (cTnT). Serum cTnT was quantified by
immunoassay. Results indicated that, in the 3.5S, 5S, and T5S groups,
serum cTnT was significantly ( P < 0.01) increased at 0 and 3 h PS. The 5S group demonstrated a greater increase in serum cTnT than
the 3.5S group ( P < 0.01) and the T5S group ( P < 0.01) at 0 h PS. Western blot analysis indicated significant decreases
( P < 0.01) in myocardial cTnT in the 5S group only at 0 h PS
( P < 0.01) and 3 h PS ( P < 0.05). Histological
evidence of localized myocyte damage demonstrated by interstitial
inflammatory infiltrates consisting of neutrophils, lymphocytes, and
histiocytes, as well as vesicular nuclei-enlarged chromatin patterns,
was observed in left ventricle specimens from the 5S group at 24 and 48 h PS. Our findings demonstrate that stressful, forced exercise induces
alterations in myocardial cTnT and that training before exercise
attenuates the exercise-induced heart damage.
myocardial damage; exercise stress; histology]]></description><subject>Anatomy & physiology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Exercise</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Health risk assessment</subject><subject>Heart</subject><subject>Heart attacks</subject><subject>Male</subject><subject>Motor Activity - physiology</subject><subject>Myocardium - metabolism</subject><subject>Physical Endurance</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Rodents</subject><subject>Stress, Physiological - blood</subject><subject>Time Factors</subject><subject>Troponin T - blood</subject><subject>Troponin T - metabolism</subject><subject>Vertebrates: cardiovascular system</subject><issn>8750-7587</issn><issn>1522-1601</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kV-L1DAUxYMo7rj6DUSCiPhg6036J82jDK4KC76MzyFNb2Y6pE1NWnbn25s6w7oIPiUkv3Nzcg4hrxnkjFX801FPk8s5AORNk1c5E6V8QjbpimesBvaUbBpRQSaqRlyRFzEeAVhZVuw5uWIgpGCF3JDjVoeu14bOwU9-7Ee6o9rNGPTc-zHSdDCcvFmhZaB67GjEkHbe0oREqm1iaZwDxmgX95FOwTs_7rFL0hnHiBTvMZg-4kvyzGoX8dVlvSY_b77stt-y2x9fv28_32ambIo5qwWwmtUdCo412q5sWy4bKYBzXUqwrYWuSuYLaItWtsYIWQouuRHMNBZ4cU3en-cmK78WjLMa-mjQOT2iX6ISjAEHBgl8-w949EsYkzfFOWcypbROK8-QCT7GgFZNoR90OCkGai1C_SlCrUWoplGVWotIsjeX2Us7YPdIdE4-Ae8ugI5GOxv0mEL6y5VcrJ98eP7Q7w93fUA1HU6xTxnvT-pmcW6H9_Nq4eFpNXU2yT78X5boR05_A7SFs8Y</recordid><startdate>20000501</startdate><enddate>20000501</enddate><creator>Chen, Yingjie</creator><creator>Serfass, Robert C</creator><creator>Mackey-Bojack, Shannon M</creator><creator>Kelly, Karen L</creator><creator>Titus, Jack L</creator><creator>Apple, Fred S</creator><general>Am Physiological Soc</general><general>American Physiological Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TS</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20000501</creationdate><title>Cardiac troponin T alterations in myocardium and serum of rats after stressful, prolonged intense exercise</title><author>Chen, Yingjie ; Serfass, Robert C ; Mackey-Bojack, Shannon M ; Kelly, Karen L ; Titus, Jack L ; Apple, Fred S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-6701616de72e6efd4bb29897022a490fbf0d571330b3b9bcc7947292c71c8f023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Anatomy & physiology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Exercise</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Health risk assessment</topic><topic>Heart</topic><topic>Heart attacks</topic><topic>Male</topic><topic>Motor Activity - physiology</topic><topic>Myocardium - metabolism</topic><topic>Physical Endurance</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Rodents</topic><topic>Stress, Physiological - blood</topic><topic>Time Factors</topic><topic>Troponin T - blood</topic><topic>Troponin T - metabolism</topic><topic>Vertebrates: cardiovascular system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Yingjie</creatorcontrib><creatorcontrib>Serfass, Robert C</creatorcontrib><creatorcontrib>Mackey-Bojack, Shannon M</creatorcontrib><creatorcontrib>Kelly, Karen L</creatorcontrib><creatorcontrib>Titus, Jack L</creatorcontrib><creatorcontrib>Apple, Fred S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Physical Education Index</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied physiology (1985)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Yingjie</au><au>Serfass, Robert C</au><au>Mackey-Bojack, Shannon M</au><au>Kelly, Karen L</au><au>Titus, Jack L</au><au>Apple, Fred S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cardiac troponin T alterations in myocardium and serum of rats after stressful, prolonged intense exercise</atitle><jtitle>Journal of applied physiology (1985)</jtitle><addtitle>J Appl Physiol (1985)</addtitle><date>2000-05-01</date><risdate>2000</risdate><volume>88</volume><issue>5</issue><spage>1749</spage><epage>1755</epage><pages>1749-1755</pages><issn>8750-7587</issn><eissn>1522-1601</eissn><coden>JAPHEV</coden><abstract><![CDATA[1 Division of Kinesiology, School of
Kinesiology and Leisure Studies, University of Minnesota, Minneapolis
55455; 2 Department of Laboratory Medicine and
Pathology, School of Medicine, University of Minnesota, Hennepin County
Medical Center, Minneapolis 55404; and
3 Jesse E. Edwards Registry of Cardiovascular
Disease, St. Paul, Minnesota 55102
The goal
of this study was to determine whether the stress of forced exercise
would result in injury to the myocardium. Male rats with 8% of body
weight attached to the tail were forced to swim 3.5 h (3.5S), forced to
swim 5 h (5S), or pretrained for 8 days and then forced to swim 5 h
(T5S). Rats were killed immediately after they swam (0 h PS) and at 3 h
(3 h PS), 24 h (24 h PS), and 48 h after they swam (48 h PS). Tissue
homogenates of the left ventricle were analyzed by Western blot
analysis for cardiac troponin T (cTnT). Serum cTnT was quantified by
immunoassay. Results indicated that, in the 3.5S, 5S, and T5S groups,
serum cTnT was significantly ( P < 0.01) increased at 0 and 3 h PS. The 5S group demonstrated a greater increase in serum cTnT than
the 3.5S group ( P < 0.01) and the T5S group ( P < 0.01) at 0 h PS. Western blot analysis indicated significant decreases
( P < 0.01) in myocardial cTnT in the 5S group only at 0 h PS
( P < 0.01) and 3 h PS ( P < 0.05). Histological
evidence of localized myocyte damage demonstrated by interstitial
inflammatory infiltrates consisting of neutrophils, lymphocytes, and
histiocytes, as well as vesicular nuclei-enlarged chromatin patterns,
was observed in left ventricle specimens from the 5S group at 24 and 48 h PS. Our findings demonstrate that stressful, forced exercise induces
alterations in myocardial cTnT and that training before exercise
attenuates the exercise-induced heart damage.
myocardial damage; exercise stress; histology]]></abstract><cop>Bethesda, MD</cop><pub>Am Physiological Soc</pub><pmid>10797139</pmid><doi>10.1152/jappl.2000.88.5.1749</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 8750-7587 1522-1601 |
| language | eng |
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| source | MEDLINE; American Physiological Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Anatomy & physiology Animals Biological and medical sciences Exercise Fundamental and applied biological sciences. Psychology Health risk assessment Heart Heart attacks Male Motor Activity - physiology Myocardium - metabolism Physical Endurance Rats Rats, Sprague-Dawley Rodents Stress, Physiological - blood Time Factors Troponin T - blood Troponin T - metabolism Vertebrates: cardiovascular system |
| title | Cardiac troponin T alterations in myocardium and serum of rats after stressful, prolonged intense exercise |
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