Role of neutrophils in xanthine/xanthine oxidase-induced oxidant injury in isolated rabbit lungs
Departments of 1 Anesthesiology and 2 Internal Medicine, and 3 Cell and Molecular Biology Program, Saint Louis University School of Medicine, St. Louis, Missouri 63110 Reactive oxygen species have been shown to play an important role in the pathogenesis of lung injury. This study was designed to...
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| Veröffentlicht in: | Journal of applied physiology (1985) 1999-12, Vol.87 (6), p.2319-2325 |
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| container_title | Journal of applied physiology (1985) |
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| creator | Kishi, Masashi Richard, Lois F Webster, Robert O Dahms, Thomas E |
| description | Departments of 1 Anesthesiology
and 2 Internal Medicine, and
3 Cell and Molecular Biology
Program, Saint Louis University School of Medicine, St. Louis, Missouri
63110
Reactive oxygen species have been shown to play
an important role in the pathogenesis of lung injury. This study was
designed to clarify the role of intrapulmonary neutrophils in the
development of xanthine/xanthine oxidase (X/XO)-induced lung injury in
isolated buffer-perfused rabbit lungs. We measured microvascular fluid filtration coefficient
( K f ) and
wet-to-dry weight ratio to assess lung injury. X/XO induced a
significant increase in
K f and wet-to-dry weight ratio in neutrophil-replete lungs, whereas the lung injury was
attenuated in neutrophil-depleted lungs. A neutrophil elastase inhibitor, ONO-5046, also attenuated the lung injury. In addition, X/XO
induced a transient pulmonary arterial pressure
(P pa ) increase. The thromboxane
inhibitor OKY-046 attenuated the
P pa increase but did not alter the
increase in permeability. Neutrophil depletion reduced the
K f increase but
had no effect on the P pa increase. These results suggest that intrapulmonary neutrophils activated by X/XO
play a major role in development of the lung injury, that neutrophil
elastase is involved in the injury, and that the X/XO-induced vasoconstriction is independent of intrapulmonary neutrophils.
permeability; neutrophil elastase; perfused lungs |
| doi_str_mv | 10.1152/jappl.1999.87.6.2319 |
| format | Article |
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and 2 Internal Medicine, and
3 Cell and Molecular Biology
Program, Saint Louis University School of Medicine, St. Louis, Missouri
63110
Reactive oxygen species have been shown to play
an important role in the pathogenesis of lung injury. This study was
designed to clarify the role of intrapulmonary neutrophils in the
development of xanthine/xanthine oxidase (X/XO)-induced lung injury in
isolated buffer-perfused rabbit lungs. We measured microvascular fluid filtration coefficient
( K f ) and
wet-to-dry weight ratio to assess lung injury. X/XO induced a
significant increase in
K f and wet-to-dry weight ratio in neutrophil-replete lungs, whereas the lung injury was
attenuated in neutrophil-depleted lungs. A neutrophil elastase inhibitor, ONO-5046, also attenuated the lung injury. In addition, X/XO
induced a transient pulmonary arterial pressure
(P pa ) increase. The thromboxane
inhibitor OKY-046 attenuated the
P pa increase but did not alter the
increase in permeability. Neutrophil depletion reduced the
K f increase but
had no effect on the P pa increase. These results suggest that intrapulmonary neutrophils activated by X/XO
play a major role in development of the lung injury, that neutrophil
elastase is involved in the injury, and that the X/XO-induced vasoconstriction is independent of intrapulmonary neutrophils.
permeability; neutrophil elastase; perfused lungs</description><identifier>ISSN: 8750-7587</identifier><identifier>EISSN: 1522-1601</identifier><identifier>DOI: 10.1152/jappl.1999.87.6.2319</identifier><identifier>PMID: 10601184</identifier><identifier>CODEN: JAPHEV</identifier><language>eng</language><publisher>Bethesda, MD: Am Physiological Soc</publisher><subject>Animals ; Biological and medical sciences ; Blood Pressure - drug effects ; Capillary Permeability - drug effects ; Drug Combinations ; Edema - chemically induced ; Edema - pathology ; Edema - physiopathology ; In Vitro Techniques ; Lung - enzymology ; Lung - pathology ; Lung - physiopathology ; Lung Diseases - chemically induced ; Lung Diseases - pathology ; Lung Diseases - physiopathology ; Lungs ; Medical sciences ; Neutrophils - physiology ; Oxidants - pharmacology ; Oxygen ; Peroxidase - metabolism ; Pneumology ; Pulmonary Artery - physiopathology ; Pulmonary Circulation - drug effects ; Rabbits ; Respiratory system : syndromes and miscellaneous diseases ; Vasoconstriction - drug effects ; Xanthine - pharmacology ; Xanthine Oxidase - pharmacology</subject><ispartof>Journal of applied physiology (1985), 1999-12, Vol.87 (6), p.2319-2325</ispartof><rights>2000 INIST-CNRS</rights><rights>Copyright American Physiological Society Dec 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c437t-67fa153e78ef391c938d91662fd83462fc0e7c95035a7e99539a42b217135bf93</citedby><cites>FETCH-LOGICAL-c437t-67fa153e78ef391c938d91662fd83462fc0e7c95035a7e99539a42b217135bf93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3039,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1230980$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10601184$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kishi, Masashi</creatorcontrib><creatorcontrib>Richard, Lois F</creatorcontrib><creatorcontrib>Webster, Robert O</creatorcontrib><creatorcontrib>Dahms, Thomas E</creatorcontrib><title>Role of neutrophils in xanthine/xanthine oxidase-induced oxidant injury in isolated rabbit lungs</title><title>Journal of applied physiology (1985)</title><addtitle>J Appl Physiol (1985)</addtitle><description>Departments of 1 Anesthesiology
and 2 Internal Medicine, and
3 Cell and Molecular Biology
Program, Saint Louis University School of Medicine, St. Louis, Missouri
63110
Reactive oxygen species have been shown to play
an important role in the pathogenesis of lung injury. This study was
designed to clarify the role of intrapulmonary neutrophils in the
development of xanthine/xanthine oxidase (X/XO)-induced lung injury in
isolated buffer-perfused rabbit lungs. We measured microvascular fluid filtration coefficient
( K f ) and
wet-to-dry weight ratio to assess lung injury. X/XO induced a
significant increase in
K f and wet-to-dry weight ratio in neutrophil-replete lungs, whereas the lung injury was
attenuated in neutrophil-depleted lungs. A neutrophil elastase inhibitor, ONO-5046, also attenuated the lung injury. In addition, X/XO
induced a transient pulmonary arterial pressure
(P pa ) increase. The thromboxane
inhibitor OKY-046 attenuated the
P pa increase but did not alter the
increase in permeability. Neutrophil depletion reduced the
K f increase but
had no effect on the P pa increase. These results suggest that intrapulmonary neutrophils activated by X/XO
play a major role in development of the lung injury, that neutrophil
elastase is involved in the injury, and that the X/XO-induced vasoconstriction is independent of intrapulmonary neutrophils.
permeability; neutrophil elastase; perfused lungs</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood Pressure - drug effects</subject><subject>Capillary Permeability - drug effects</subject><subject>Drug Combinations</subject><subject>Edema - chemically induced</subject><subject>Edema - pathology</subject><subject>Edema - physiopathology</subject><subject>In Vitro Techniques</subject><subject>Lung - enzymology</subject><subject>Lung - pathology</subject><subject>Lung - physiopathology</subject><subject>Lung Diseases - chemically induced</subject><subject>Lung Diseases - pathology</subject><subject>Lung Diseases - physiopathology</subject><subject>Lungs</subject><subject>Medical sciences</subject><subject>Neutrophils - physiology</subject><subject>Oxidants - pharmacology</subject><subject>Oxygen</subject><subject>Peroxidase - metabolism</subject><subject>Pneumology</subject><subject>Pulmonary Artery - physiopathology</subject><subject>Pulmonary Circulation - drug effects</subject><subject>Rabbits</subject><subject>Respiratory system : syndromes and miscellaneous diseases</subject><subject>Vasoconstriction - drug effects</subject><subject>Xanthine - pharmacology</subject><subject>Xanthine Oxidase - pharmacology</subject><issn>8750-7587</issn><issn>1522-1601</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE9v1DAQxS0EotvCN0AoQhx6Seq_sX1EFQWkSkionI2T2BuvXDvYibr77XHYBfbCaWY0vzdv9AB4g2CDEMM3Oz1NvkFSykbwpm0wQfIZ2JQVrlEL0XOwEZzBmjPBL8BlzjsIEaUMvQQXCBYACboBP75Fb6poq2CWOcVpdD5XLlR7HebRBXPzp6ni3g06m9qFYenNcJzDXODdkg6rxuXo9VxWSXedmyu_hG1-BV5Y7bN5fapX4Pvdx4fbz_X9109fbj_c1z0lfK5bbjVixHBhLJGol0QMErUttoMgtJQeGt5LBgnT3EjJiNQUdxhxRFhnJbkC7453pxR_LibPaheXFIqlwhgjJimFBaJHqE8x52SsmpJ71OmgEFRrqup3qmpNVQmuWrWmWmRvT7eX7tEMZ6JjjAV4fwJ07rW3SYfe5X8cJlCKM_vRbccnl4yaxkN20cftQd0t3j-Y_by-8NdaTYMtsuv_ywp99ukvoJ2iwg</recordid><startdate>19991201</startdate><enddate>19991201</enddate><creator>Kishi, Masashi</creator><creator>Richard, Lois F</creator><creator>Webster, Robert O</creator><creator>Dahms, Thomas E</creator><general>Am Physiological Soc</general><general>American Physiological Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TS</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>19991201</creationdate><title>Role of neutrophils in xanthine/xanthine oxidase-induced oxidant injury in isolated rabbit lungs</title><author>Kishi, Masashi ; Richard, Lois F ; Webster, Robert O ; Dahms, Thomas E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c437t-67fa153e78ef391c938d91662fd83462fc0e7c95035a7e99539a42b217135bf93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood Pressure - drug effects</topic><topic>Capillary Permeability - drug effects</topic><topic>Drug Combinations</topic><topic>Edema - chemically induced</topic><topic>Edema - pathology</topic><topic>Edema - physiopathology</topic><topic>In Vitro Techniques</topic><topic>Lung - enzymology</topic><topic>Lung - pathology</topic><topic>Lung - physiopathology</topic><topic>Lung Diseases - chemically induced</topic><topic>Lung Diseases - pathology</topic><topic>Lung Diseases - physiopathology</topic><topic>Lungs</topic><topic>Medical sciences</topic><topic>Neutrophils - physiology</topic><topic>Oxidants - pharmacology</topic><topic>Oxygen</topic><topic>Peroxidase - metabolism</topic><topic>Pneumology</topic><topic>Pulmonary Artery - physiopathology</topic><topic>Pulmonary Circulation - drug effects</topic><topic>Rabbits</topic><topic>Respiratory system : syndromes and miscellaneous diseases</topic><topic>Vasoconstriction - drug effects</topic><topic>Xanthine - pharmacology</topic><topic>Xanthine Oxidase - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kishi, Masashi</creatorcontrib><creatorcontrib>Richard, Lois F</creatorcontrib><creatorcontrib>Webster, Robert O</creatorcontrib><creatorcontrib>Dahms, Thomas E</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Physical Education Index</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of applied physiology (1985)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kishi, Masashi</au><au>Richard, Lois F</au><au>Webster, Robert O</au><au>Dahms, Thomas E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of neutrophils in xanthine/xanthine oxidase-induced oxidant injury in isolated rabbit lungs</atitle><jtitle>Journal of applied physiology (1985)</jtitle><addtitle>J Appl Physiol (1985)</addtitle><date>1999-12-01</date><risdate>1999</risdate><volume>87</volume><issue>6</issue><spage>2319</spage><epage>2325</epage><pages>2319-2325</pages><issn>8750-7587</issn><eissn>1522-1601</eissn><coden>JAPHEV</coden><abstract>Departments of 1 Anesthesiology
and 2 Internal Medicine, and
3 Cell and Molecular Biology
Program, Saint Louis University School of Medicine, St. Louis, Missouri
63110
Reactive oxygen species have been shown to play
an important role in the pathogenesis of lung injury. This study was
designed to clarify the role of intrapulmonary neutrophils in the
development of xanthine/xanthine oxidase (X/XO)-induced lung injury in
isolated buffer-perfused rabbit lungs. We measured microvascular fluid filtration coefficient
( K f ) and
wet-to-dry weight ratio to assess lung injury. X/XO induced a
significant increase in
K f and wet-to-dry weight ratio in neutrophil-replete lungs, whereas the lung injury was
attenuated in neutrophil-depleted lungs. A neutrophil elastase inhibitor, ONO-5046, also attenuated the lung injury. In addition, X/XO
induced a transient pulmonary arterial pressure
(P pa ) increase. The thromboxane
inhibitor OKY-046 attenuated the
P pa increase but did not alter the
increase in permeability. Neutrophil depletion reduced the
K f increase but
had no effect on the P pa increase. These results suggest that intrapulmonary neutrophils activated by X/XO
play a major role in development of the lung injury, that neutrophil
elastase is involved in the injury, and that the X/XO-induced vasoconstriction is independent of intrapulmonary neutrophils.
permeability; neutrophil elastase; perfused lungs</abstract><cop>Bethesda, MD</cop><pub>Am Physiological Soc</pub><pmid>10601184</pmid><doi>10.1152/jappl.1999.87.6.2319</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 8750-7587 |
| ispartof | Journal of applied physiology (1985), 1999-12, Vol.87 (6), p.2319-2325 |
| issn | 8750-7587 1522-1601 |
| language | eng |
| recordid | cdi_proquest_journals_222159440 |
| source | MEDLINE; American Physiological Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Biological and medical sciences Blood Pressure - drug effects Capillary Permeability - drug effects Drug Combinations Edema - chemically induced Edema - pathology Edema - physiopathology In Vitro Techniques Lung - enzymology Lung - pathology Lung - physiopathology Lung Diseases - chemically induced Lung Diseases - pathology Lung Diseases - physiopathology Lungs Medical sciences Neutrophils - physiology Oxidants - pharmacology Oxygen Peroxidase - metabolism Pneumology Pulmonary Artery - physiopathology Pulmonary Circulation - drug effects Rabbits Respiratory system : syndromes and miscellaneous diseases Vasoconstriction - drug effects Xanthine - pharmacology Xanthine Oxidase - pharmacology |
| title | Role of neutrophils in xanthine/xanthine oxidase-induced oxidant injury in isolated rabbit lungs |
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