Differential microvascular response to disuse in rat hindlimb skeletal muscles
1 A. C. Burton Vascular Biology Laboratory, London Health Sciences Research, and Department of Medical Biophysics; 2 Faculty of Kinesiology, The University of Western Ontario, London, Ontario, Canada N6A 5C1; and 3 Department of Medicine, University of California-San Diego, La Jolla, California 9...
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creator | Tyml, Karel Mathieu-Costello, Odile Cheng, Linong Noble, Earl G |
description | 1 A. C. Burton Vascular Biology
Laboratory, London Health Sciences Research, and Department of
Medical Biophysics; 2 Faculty of
Kinesiology, The University of Western Ontario, London, Ontario,
Canada N6A 5C1; and 3 Department
of Medicine, University of California-San Diego, La Jolla,
California 92093-0623
The aim of the
study was to address discrepant findings in the literature regarding
coupling between decreased functional demand during disuse and reduced
capillarity. We previously reported [K. Tyml, O. Mathieu-Costello, and E. Noble. Microvasc.
Res. 49: 17-32, 1995] that severe disuse of
rat extensor digitorum longus (EDL) muscle caused by a 2-wk application
of tetrodotoxin (TTX) on the sciatic nerve is not accompanied by
capillary loss. Using the same animal model, the present study examined
whether this absence of coupling could be explained in terms of
1 ) too short a duration of disuse
and 2 ) muscle-specific response to
disuse. Fischer 344 rats were exposed to either no treatment (control) or to 2- or 8-wk TTX applications. Fiber size, capillary density per
fiber cross-sectional area, and capillary-to-fiber (C/F) ratio were
determined by morphometry in the EDL muscle (control, 2- and 8-wk
groups) and in the superficial portion of medial gastrocnemius (Gas)
muscle (control, 2 wk). In both muscles, microvascular blood flow was
evaluated by intravital microscopy [red blood cell
velocity in capillaries
( V RBC )]
and by laser Doppler flowmetry (LDF). Regardless of duration of TTX
application or muscle type, TTX-induced disuse resulted in a
significant reduction of fiber area (44-71%). However, capillary
density increased in EDL muscle (both at 2 and 8 wk) but not in Gas
muscle. C/F ratio decreased in EDL muscle at 8 wk (18%) and in Gas
muscle (39%). This indicates that the effect on capillarity depended
on duration of disuse and on muscle type. V RBC and LDF
signal were significantly larger in EDL than in Gas muscle. Analysis of
change in capillarity vs.
V RBC suggested
that the outcome of disuse may be modulated by blood flow. We conclude that the duration of skeletal muscle disuse per se does not dictate capillary loss, and we hypothesize that discrepant findings of coupling
between functional demand and capillarity could be due to the
presence/absence of flow-related angiogenesis superimposed on the
capillary removal process during disuse.
capillary density; blood flow; atrophy; capillary damage; angiogenesis |
doi_str_mv | 10.1152/jappl.1999.87.4.1496 |
format | Article |
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Laboratory, London Health Sciences Research, and Department of
Medical Biophysics; 2 Faculty of
Kinesiology, The University of Western Ontario, London, Ontario,
Canada N6A 5C1; and 3 Department
of Medicine, University of California-San Diego, La Jolla,
California 92093-0623
The aim of the
study was to address discrepant findings in the literature regarding
coupling between decreased functional demand during disuse and reduced
capillarity. We previously reported [K. Tyml, O. Mathieu-Costello, and E. Noble. Microvasc.
Res. 49: 17-32, 1995] that severe disuse of
rat extensor digitorum longus (EDL) muscle caused by a 2-wk application
of tetrodotoxin (TTX) on the sciatic nerve is not accompanied by
capillary loss. Using the same animal model, the present study examined
whether this absence of coupling could be explained in terms of
1 ) too short a duration of disuse
and 2 ) muscle-specific response to
disuse. Fischer 344 rats were exposed to either no treatment (control) or to 2- or 8-wk TTX applications. Fiber size, capillary density per
fiber cross-sectional area, and capillary-to-fiber (C/F) ratio were
determined by morphometry in the EDL muscle (control, 2- and 8-wk
groups) and in the superficial portion of medial gastrocnemius (Gas)
muscle (control, 2 wk). In both muscles, microvascular blood flow was
evaluated by intravital microscopy [red blood cell
velocity in capillaries
( V RBC )]
and by laser Doppler flowmetry (LDF). Regardless of duration of TTX
application or muscle type, TTX-induced disuse resulted in a
significant reduction of fiber area (44-71%). However, capillary
density increased in EDL muscle (both at 2 and 8 wk) but not in Gas
muscle. C/F ratio decreased in EDL muscle at 8 wk (18%) and in Gas
muscle (39%). This indicates that the effect on capillarity depended
on duration of disuse and on muscle type. V RBC and LDF
signal were significantly larger in EDL than in Gas muscle. Analysis of
change in capillarity vs.
V RBC suggested
that the outcome of disuse may be modulated by blood flow. We conclude that the duration of skeletal muscle disuse per se does not dictate capillary loss, and we hypothesize that discrepant findings of coupling
between functional demand and capillarity could be due to the
presence/absence of flow-related angiogenesis superimposed on the
capillary removal process during disuse.
capillary density; blood flow; atrophy; capillary damage; angiogenesis</description><identifier>ISSN: 8750-7587</identifier><identifier>EISSN: 1522-1601</identifier><identifier>DOI: 10.1152/jappl.1999.87.4.1496</identifier><identifier>PMID: 10517784</identifier><identifier>CODEN: JAPHEV</identifier><language>eng</language><publisher>Bethesda, MD: Am Physiological Soc</publisher><subject>Anatomy & physiology ; Animals ; Biological and medical sciences ; Blood vessels ; Blood Volume - drug effects ; Body Weight - drug effects ; Capillaries - pathology ; Circulatory system ; Fundamental and applied biological sciences. Psychology ; Hemodynamics - drug effects ; Hindlimb ; Ischemia - physiopathology ; Male ; Microcirculation - drug effects ; Microcirculation - physiology ; Muscle, Skeletal - blood supply ; Muscle, Skeletal - pathology ; Muscle, Skeletal - physiology ; Muscle, Skeletal - physiopathology ; Muscular Atrophy - chemically induced ; Muscular Atrophy - pathology ; Muscular Atrophy - physiopathology ; Muscular system ; Rats ; Rats, Inbred F344 ; Rodents ; Sciatic Nerve - drug effects ; Striated muscle. Tendons ; Tetrodotoxin - pharmacology ; Time Factors ; Vertebrates: osteoarticular system, musculoskeletal system</subject><ispartof>Journal of applied physiology (1985), 1999-10, Vol.87 (4), p.1496-1505</ispartof><rights>1999 INIST-CNRS</rights><rights>Copyright American Physiological Society Oct 1999</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c534t-330cf4e70022b3f1ffd790904e7efe0de3834ff9f37f6b4e8170255b1bdf78213</citedby><cites>FETCH-LOGICAL-c534t-330cf4e70022b3f1ffd790904e7efe0de3834ff9f37f6b4e8170255b1bdf78213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3025,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1993345$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10517784$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tyml, Karel</creatorcontrib><creatorcontrib>Mathieu-Costello, Odile</creatorcontrib><creatorcontrib>Cheng, Linong</creatorcontrib><creatorcontrib>Noble, Earl G</creatorcontrib><title>Differential microvascular response to disuse in rat hindlimb skeletal muscles</title><title>Journal of applied physiology (1985)</title><addtitle>J Appl Physiol (1985)</addtitle><description>1 A. C. Burton Vascular Biology
Laboratory, London Health Sciences Research, and Department of
Medical Biophysics; 2 Faculty of
Kinesiology, The University of Western Ontario, London, Ontario,
Canada N6A 5C1; and 3 Department
of Medicine, University of California-San Diego, La Jolla,
California 92093-0623
The aim of the
study was to address discrepant findings in the literature regarding
coupling between decreased functional demand during disuse and reduced
capillarity. We previously reported [K. Tyml, O. Mathieu-Costello, and E. Noble. Microvasc.
Res. 49: 17-32, 1995] that severe disuse of
rat extensor digitorum longus (EDL) muscle caused by a 2-wk application
of tetrodotoxin (TTX) on the sciatic nerve is not accompanied by
capillary loss. Using the same animal model, the present study examined
whether this absence of coupling could be explained in terms of
1 ) too short a duration of disuse
and 2 ) muscle-specific response to
disuse. Fischer 344 rats were exposed to either no treatment (control) or to 2- or 8-wk TTX applications. Fiber size, capillary density per
fiber cross-sectional area, and capillary-to-fiber (C/F) ratio were
determined by morphometry in the EDL muscle (control, 2- and 8-wk
groups) and in the superficial portion of medial gastrocnemius (Gas)
muscle (control, 2 wk). In both muscles, microvascular blood flow was
evaluated by intravital microscopy [red blood cell
velocity in capillaries
( V RBC )]
and by laser Doppler flowmetry (LDF). Regardless of duration of TTX
application or muscle type, TTX-induced disuse resulted in a
significant reduction of fiber area (44-71%). However, capillary
density increased in EDL muscle (both at 2 and 8 wk) but not in Gas
muscle. C/F ratio decreased in EDL muscle at 8 wk (18%) and in Gas
muscle (39%). This indicates that the effect on capillarity depended
on duration of disuse and on muscle type. V RBC and LDF
signal were significantly larger in EDL than in Gas muscle. Analysis of
change in capillarity vs.
V RBC suggested
that the outcome of disuse may be modulated by blood flow. We conclude that the duration of skeletal muscle disuse per se does not dictate capillary loss, and we hypothesize that discrepant findings of coupling
between functional demand and capillarity could be due to the
presence/absence of flow-related angiogenesis superimposed on the
capillary removal process during disuse.
capillary density; blood flow; atrophy; capillary damage; angiogenesis</description><subject>Anatomy & physiology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood vessels</subject><subject>Blood Volume - drug effects</subject><subject>Body Weight - drug effects</subject><subject>Capillaries - pathology</subject><subject>Circulatory system</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hemodynamics - drug effects</subject><subject>Hindlimb</subject><subject>Ischemia - physiopathology</subject><subject>Male</subject><subject>Microcirculation - drug effects</subject><subject>Microcirculation - physiology</subject><subject>Muscle, Skeletal - blood supply</subject><subject>Muscle, Skeletal - pathology</subject><subject>Muscle, Skeletal - physiology</subject><subject>Muscle, Skeletal - physiopathology</subject><subject>Muscular Atrophy - chemically induced</subject><subject>Muscular Atrophy - pathology</subject><subject>Muscular Atrophy - physiopathology</subject><subject>Muscular system</subject><subject>Rats</subject><subject>Rats, Inbred F344</subject><subject>Rodents</subject><subject>Sciatic Nerve - drug effects</subject><subject>Striated muscle. Tendons</subject><subject>Tetrodotoxin - pharmacology</subject><subject>Time Factors</subject><subject>Vertebrates: osteoarticular system, musculoskeletal system</subject><issn>8750-7587</issn><issn>1522-1601</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1v1DAQhi0EokvhHyAUIVRxSfDYzto-otICUgWXcracZNz14nxgJ8D-exx2xZfEySP7eWc8DyFPgVYANXu1t9MUKtBaV0pWogKht_fIJj-xErYU7pONkjUtZa3kGXmU0p5SEKKGh-QMaA1SKrEhH9545zDiMHsbit63cfxqU7sEG4uIaRqHhMU8Fp1PS678UEQ7Fzs_dMH3TZE-Y8B5TS6pDZgekwfOhoRPTuc5-XR9dXv5rrz5-Pb95eubsq25mEvOaesESkoZa7gD5zqpqab5Ch3SDrniwjntuHTbRqACSVldN9B0TioG_JxcHPtOcfyyYJpN71OLIdgBxyUZttUMqFYZfP4PuB-XOOS_GcZY9gGKZkgcobx9ShGdmaLvbTwYoGaVbX7KNqtso6QRZpWdY89OvZemx-6P0NFuBl6cgKzUBhft0Pr0m9Oac1Fn7OUR2_m73Tcf0Uy7Q_JjGO8O6-S_Ror_o9dLCLf4fV4zvyJm6hz_AQH5ql8</recordid><startdate>19991001</startdate><enddate>19991001</enddate><creator>Tyml, Karel</creator><creator>Mathieu-Costello, Odile</creator><creator>Cheng, Linong</creator><creator>Noble, Earl G</creator><general>Am Physiological Soc</general><general>American Physiological Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TS</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>H8D</scope><scope>L7M</scope></search><sort><creationdate>19991001</creationdate><title>Differential microvascular response to disuse in rat hindlimb skeletal muscles</title><author>Tyml, Karel ; Mathieu-Costello, Odile ; Cheng, Linong ; Noble, Earl G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c534t-330cf4e70022b3f1ffd790904e7efe0de3834ff9f37f6b4e8170255b1bdf78213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Anatomy & physiology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood vessels</topic><topic>Blood Volume - drug effects</topic><topic>Body Weight - drug effects</topic><topic>Capillaries - pathology</topic><topic>Circulatory system</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hemodynamics - drug effects</topic><topic>Hindlimb</topic><topic>Ischemia - physiopathology</topic><topic>Male</topic><topic>Microcirculation - drug effects</topic><topic>Microcirculation - physiology</topic><topic>Muscle, Skeletal - blood supply</topic><topic>Muscle, Skeletal - pathology</topic><topic>Muscle, Skeletal - physiology</topic><topic>Muscle, Skeletal - physiopathology</topic><topic>Muscular Atrophy - chemically induced</topic><topic>Muscular Atrophy - pathology</topic><topic>Muscular Atrophy - physiopathology</topic><topic>Muscular system</topic><topic>Rats</topic><topic>Rats, Inbred F344</topic><topic>Rodents</topic><topic>Sciatic Nerve - drug effects</topic><topic>Striated muscle. Tendons</topic><topic>Tetrodotoxin - pharmacology</topic><topic>Time Factors</topic><topic>Vertebrates: osteoarticular system, musculoskeletal system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tyml, Karel</creatorcontrib><creatorcontrib>Mathieu-Costello, Odile</creatorcontrib><creatorcontrib>Cheng, Linong</creatorcontrib><creatorcontrib>Noble, Earl G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Physical Education Index</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Aerospace Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Journal of applied physiology (1985)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tyml, Karel</au><au>Mathieu-Costello, Odile</au><au>Cheng, Linong</au><au>Noble, Earl G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential microvascular response to disuse in rat hindlimb skeletal muscles</atitle><jtitle>Journal of applied physiology (1985)</jtitle><addtitle>J Appl Physiol (1985)</addtitle><date>1999-10-01</date><risdate>1999</risdate><volume>87</volume><issue>4</issue><spage>1496</spage><epage>1505</epage><pages>1496-1505</pages><issn>8750-7587</issn><eissn>1522-1601</eissn><coden>JAPHEV</coden><abstract>1 A. C. Burton Vascular Biology
Laboratory, London Health Sciences Research, and Department of
Medical Biophysics; 2 Faculty of
Kinesiology, The University of Western Ontario, London, Ontario,
Canada N6A 5C1; and 3 Department
of Medicine, University of California-San Diego, La Jolla,
California 92093-0623
The aim of the
study was to address discrepant findings in the literature regarding
coupling between decreased functional demand during disuse and reduced
capillarity. We previously reported [K. Tyml, O. Mathieu-Costello, and E. Noble. Microvasc.
Res. 49: 17-32, 1995] that severe disuse of
rat extensor digitorum longus (EDL) muscle caused by a 2-wk application
of tetrodotoxin (TTX) on the sciatic nerve is not accompanied by
capillary loss. Using the same animal model, the present study examined
whether this absence of coupling could be explained in terms of
1 ) too short a duration of disuse
and 2 ) muscle-specific response to
disuse. Fischer 344 rats were exposed to either no treatment (control) or to 2- or 8-wk TTX applications. Fiber size, capillary density per
fiber cross-sectional area, and capillary-to-fiber (C/F) ratio were
determined by morphometry in the EDL muscle (control, 2- and 8-wk
groups) and in the superficial portion of medial gastrocnemius (Gas)
muscle (control, 2 wk). In both muscles, microvascular blood flow was
evaluated by intravital microscopy [red blood cell
velocity in capillaries
( V RBC )]
and by laser Doppler flowmetry (LDF). Regardless of duration of TTX
application or muscle type, TTX-induced disuse resulted in a
significant reduction of fiber area (44-71%). However, capillary
density increased in EDL muscle (both at 2 and 8 wk) but not in Gas
muscle. C/F ratio decreased in EDL muscle at 8 wk (18%) and in Gas
muscle (39%). This indicates that the effect on capillarity depended
on duration of disuse and on muscle type. V RBC and LDF
signal were significantly larger in EDL than in Gas muscle. Analysis of
change in capillarity vs.
V RBC suggested
that the outcome of disuse may be modulated by blood flow. We conclude that the duration of skeletal muscle disuse per se does not dictate capillary loss, and we hypothesize that discrepant findings of coupling
between functional demand and capillarity could be due to the
presence/absence of flow-related angiogenesis superimposed on the
capillary removal process during disuse.
capillary density; blood flow; atrophy; capillary damage; angiogenesis</abstract><cop>Bethesda, MD</cop><pub>Am Physiological Soc</pub><pmid>10517784</pmid><doi>10.1152/jappl.1999.87.4.1496</doi><tpages>10</tpages></addata></record> |
fulltext | fulltext |
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ispartof | Journal of applied physiology (1985), 1999-10, Vol.87 (4), p.1496-1505 |
issn | 8750-7587 1522-1601 |
language | eng |
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source | MEDLINE; American Physiological Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
subjects | Anatomy & physiology Animals Biological and medical sciences Blood vessels Blood Volume - drug effects Body Weight - drug effects Capillaries - pathology Circulatory system Fundamental and applied biological sciences. Psychology Hemodynamics - drug effects Hindlimb Ischemia - physiopathology Male Microcirculation - drug effects Microcirculation - physiology Muscle, Skeletal - blood supply Muscle, Skeletal - pathology Muscle, Skeletal - physiology Muscle, Skeletal - physiopathology Muscular Atrophy - chemically induced Muscular Atrophy - pathology Muscular Atrophy - physiopathology Muscular system Rats Rats, Inbred F344 Rodents Sciatic Nerve - drug effects Striated muscle. Tendons Tetrodotoxin - pharmacology Time Factors Vertebrates: osteoarticular system, musculoskeletal system |
title | Differential microvascular response to disuse in rat hindlimb skeletal muscles |
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