Differential microvascular response to disuse in rat hindlimb skeletal muscles

1  A. C. Burton Vascular Biology Laboratory, London Health Sciences Research, and Department of Medical Biophysics; 2  Faculty of Kinesiology, The University of Western Ontario, London, Ontario, Canada N6A 5C1; and 3  Department of Medicine, University of California-San Diego, La Jolla, California 9...

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Veröffentlicht in:Journal of applied physiology (1985) 1999-10, Vol.87 (4), p.1496-1505
Hauptverfasser: Tyml, Karel, Mathieu-Costello, Odile, Cheng, Linong, Noble, Earl G
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container_title Journal of applied physiology (1985)
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creator Tyml, Karel
Mathieu-Costello, Odile
Cheng, Linong
Noble, Earl G
description 1  A. C. Burton Vascular Biology Laboratory, London Health Sciences Research, and Department of Medical Biophysics; 2  Faculty of Kinesiology, The University of Western Ontario, London, Ontario, Canada N6A 5C1; and 3  Department of Medicine, University of California-San Diego, La Jolla, California 92093-0623 The aim of the study was to address discrepant findings in the literature regarding coupling between decreased functional demand during disuse and reduced capillarity. We previously reported [K. Tyml, O. Mathieu-Costello, and E. Noble. Microvasc. Res. 49: 17-32, 1995] that severe disuse of rat extensor digitorum longus (EDL) muscle caused by a 2-wk application of tetrodotoxin (TTX) on the sciatic nerve is not accompanied by capillary loss. Using the same animal model, the present study examined whether this absence of coupling could be explained in terms of 1 ) too short a duration of disuse and 2 ) muscle-specific response to disuse. Fischer 344 rats were exposed to either no treatment (control) or to 2- or 8-wk TTX applications. Fiber size, capillary density per fiber cross-sectional area, and capillary-to-fiber (C/F) ratio were determined by morphometry in the EDL muscle (control, 2-   and 8-wk groups) and in the superficial portion of medial gastrocnemius (Gas) muscle (control, 2 wk). In both muscles, microvascular blood flow was evaluated by intravital microscopy [red blood cell velocity in capillaries ( V RBC )] and by laser Doppler flowmetry (LDF). Regardless of duration of TTX application or muscle type, TTX-induced disuse resulted in a significant reduction of fiber area (44-71%). However, capillary density increased in EDL muscle (both at 2 and 8 wk) but not in Gas muscle. C/F ratio decreased in EDL muscle at 8   wk (18%) and in Gas muscle (39%). This indicates that the effect on capillarity depended on duration of disuse and on muscle type. V RBC and LDF signal were significantly larger in EDL than in Gas muscle. Analysis of change in capillarity vs. V RBC suggested that the outcome of disuse may be modulated by blood flow. We conclude that the duration of skeletal muscle disuse per se does not dictate capillary loss, and we hypothesize that discrepant findings of coupling between functional demand and capillarity could be due to the presence/absence of flow-related angiogenesis superimposed on the capillary removal process during disuse. capillary density; blood flow; atrophy; capillary damage; angiogenesis
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C. Burton Vascular Biology Laboratory, London Health Sciences Research, and Department of Medical Biophysics; 2  Faculty of Kinesiology, The University of Western Ontario, London, Ontario, Canada N6A 5C1; and 3  Department of Medicine, University of California-San Diego, La Jolla, California 92093-0623 The aim of the study was to address discrepant findings in the literature regarding coupling between decreased functional demand during disuse and reduced capillarity. We previously reported [K. Tyml, O. Mathieu-Costello, and E. Noble. Microvasc. Res. 49: 17-32, 1995] that severe disuse of rat extensor digitorum longus (EDL) muscle caused by a 2-wk application of tetrodotoxin (TTX) on the sciatic nerve is not accompanied by capillary loss. Using the same animal model, the present study examined whether this absence of coupling could be explained in terms of 1 ) too short a duration of disuse and 2 ) muscle-specific response to disuse. Fischer 344 rats were exposed to either no treatment (control) or to 2- or 8-wk TTX applications. Fiber size, capillary density per fiber cross-sectional area, and capillary-to-fiber (C/F) ratio were determined by morphometry in the EDL muscle (control, 2-   and 8-wk groups) and in the superficial portion of medial gastrocnemius (Gas) muscle (control, 2 wk). In both muscles, microvascular blood flow was evaluated by intravital microscopy [red blood cell velocity in capillaries ( V RBC )] and by laser Doppler flowmetry (LDF). Regardless of duration of TTX application or muscle type, TTX-induced disuse resulted in a significant reduction of fiber area (44-71%). However, capillary density increased in EDL muscle (both at 2 and 8 wk) but not in Gas muscle. C/F ratio decreased in EDL muscle at 8   wk (18%) and in Gas muscle (39%). This indicates that the effect on capillarity depended on duration of disuse and on muscle type. 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Psychology ; Hemodynamics - drug effects ; Hindlimb ; Ischemia - physiopathology ; Male ; Microcirculation - drug effects ; Microcirculation - physiology ; Muscle, Skeletal - blood supply ; Muscle, Skeletal - pathology ; Muscle, Skeletal - physiology ; Muscle, Skeletal - physiopathology ; Muscular Atrophy - chemically induced ; Muscular Atrophy - pathology ; Muscular Atrophy - physiopathology ; Muscular system ; Rats ; Rats, Inbred F344 ; Rodents ; Sciatic Nerve - drug effects ; Striated muscle. 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C. Burton Vascular Biology Laboratory, London Health Sciences Research, and Department of Medical Biophysics; 2  Faculty of Kinesiology, The University of Western Ontario, London, Ontario, Canada N6A 5C1; and 3  Department of Medicine, University of California-San Diego, La Jolla, California 92093-0623 The aim of the study was to address discrepant findings in the literature regarding coupling between decreased functional demand during disuse and reduced capillarity. We previously reported [K. Tyml, O. Mathieu-Costello, and E. Noble. Microvasc. Res. 49: 17-32, 1995] that severe disuse of rat extensor digitorum longus (EDL) muscle caused by a 2-wk application of tetrodotoxin (TTX) on the sciatic nerve is not accompanied by capillary loss. Using the same animal model, the present study examined whether this absence of coupling could be explained in terms of 1 ) too short a duration of disuse and 2 ) muscle-specific response to disuse. Fischer 344 rats were exposed to either no treatment (control) or to 2- or 8-wk TTX applications. Fiber size, capillary density per fiber cross-sectional area, and capillary-to-fiber (C/F) ratio were determined by morphometry in the EDL muscle (control, 2-   and 8-wk groups) and in the superficial portion of medial gastrocnemius (Gas) muscle (control, 2 wk). In both muscles, microvascular blood flow was evaluated by intravital microscopy [red blood cell velocity in capillaries ( V RBC )] and by laser Doppler flowmetry (LDF). Regardless of duration of TTX application or muscle type, TTX-induced disuse resulted in a significant reduction of fiber area (44-71%). However, capillary density increased in EDL muscle (both at 2 and 8 wk) but not in Gas muscle. C/F ratio decreased in EDL muscle at 8   wk (18%) and in Gas muscle (39%). This indicates that the effect on capillarity depended on duration of disuse and on muscle type. V RBC and LDF signal were significantly larger in EDL than in Gas muscle. Analysis of change in capillarity vs. V RBC suggested that the outcome of disuse may be modulated by blood flow. We conclude that the duration of skeletal muscle disuse per se does not dictate capillary loss, and we hypothesize that discrepant findings of coupling between functional demand and capillarity could be due to the presence/absence of flow-related angiogenesis superimposed on the capillary removal process during disuse. capillary density; blood flow; atrophy; capillary damage; angiogenesis</description><subject>Anatomy &amp; physiology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood vessels</subject><subject>Blood Volume - drug effects</subject><subject>Body Weight - drug effects</subject><subject>Capillaries - pathology</subject><subject>Circulatory system</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hemodynamics - drug effects</subject><subject>Hindlimb</subject><subject>Ischemia - physiopathology</subject><subject>Male</subject><subject>Microcirculation - drug effects</subject><subject>Microcirculation - physiology</subject><subject>Muscle, Skeletal - blood supply</subject><subject>Muscle, Skeletal - pathology</subject><subject>Muscle, Skeletal - physiology</subject><subject>Muscle, Skeletal - physiopathology</subject><subject>Muscular Atrophy - chemically induced</subject><subject>Muscular Atrophy - pathology</subject><subject>Muscular Atrophy - physiopathology</subject><subject>Muscular system</subject><subject>Rats</subject><subject>Rats, Inbred F344</subject><subject>Rodents</subject><subject>Sciatic Nerve - drug effects</subject><subject>Striated muscle. Tendons</subject><subject>Tetrodotoxin - pharmacology</subject><subject>Time Factors</subject><subject>Vertebrates: osteoarticular system, musculoskeletal system</subject><issn>8750-7587</issn><issn>1522-1601</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1v1DAQhi0EokvhHyAUIVRxSfDYzto-otICUgWXcracZNz14nxgJ8D-exx2xZfEySP7eWc8DyFPgVYANXu1t9MUKtBaV0pWogKht_fIJj-xErYU7pONkjUtZa3kGXmU0p5SEKKGh-QMaA1SKrEhH9545zDiMHsbit63cfxqU7sEG4uIaRqHhMU8Fp1PS678UEQ7Fzs_dMH3TZE-Y8B5TS6pDZgekwfOhoRPTuc5-XR9dXv5rrz5-Pb95eubsq25mEvOaesESkoZa7gD5zqpqab5Ch3SDrniwjntuHTbRqACSVldN9B0TioG_JxcHPtOcfyyYJpN71OLIdgBxyUZttUMqFYZfP4PuB-XOOS_GcZY9gGKZkgcobx9ShGdmaLvbTwYoGaVbX7KNqtso6QRZpWdY89OvZemx-6P0NFuBl6cgKzUBhft0Pr0m9Oac1Fn7OUR2_m73Tcf0Uy7Q_JjGO8O6-S_Ror_o9dLCLf4fV4zvyJm6hz_AQH5ql8</recordid><startdate>19991001</startdate><enddate>19991001</enddate><creator>Tyml, Karel</creator><creator>Mathieu-Costello, Odile</creator><creator>Cheng, Linong</creator><creator>Noble, Earl G</creator><general>Am Physiological Soc</general><general>American Physiological Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TS</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>H8D</scope><scope>L7M</scope></search><sort><creationdate>19991001</creationdate><title>Differential microvascular response to disuse in rat hindlimb skeletal muscles</title><author>Tyml, Karel ; Mathieu-Costello, Odile ; Cheng, Linong ; Noble, Earl G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c534t-330cf4e70022b3f1ffd790904e7efe0de3834ff9f37f6b4e8170255b1bdf78213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Anatomy &amp; physiology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood vessels</topic><topic>Blood Volume - drug effects</topic><topic>Body Weight - drug effects</topic><topic>Capillaries - pathology</topic><topic>Circulatory system</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hemodynamics - drug effects</topic><topic>Hindlimb</topic><topic>Ischemia - physiopathology</topic><topic>Male</topic><topic>Microcirculation - drug effects</topic><topic>Microcirculation - physiology</topic><topic>Muscle, Skeletal - blood supply</topic><topic>Muscle, Skeletal - pathology</topic><topic>Muscle, Skeletal - physiology</topic><topic>Muscle, Skeletal - physiopathology</topic><topic>Muscular Atrophy - chemically induced</topic><topic>Muscular Atrophy - pathology</topic><topic>Muscular Atrophy - physiopathology</topic><topic>Muscular system</topic><topic>Rats</topic><topic>Rats, Inbred F344</topic><topic>Rodents</topic><topic>Sciatic Nerve - drug effects</topic><topic>Striated muscle. 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C. Burton Vascular Biology Laboratory, London Health Sciences Research, and Department of Medical Biophysics; 2  Faculty of Kinesiology, The University of Western Ontario, London, Ontario, Canada N6A 5C1; and 3  Department of Medicine, University of California-San Diego, La Jolla, California 92093-0623 The aim of the study was to address discrepant findings in the literature regarding coupling between decreased functional demand during disuse and reduced capillarity. We previously reported [K. Tyml, O. Mathieu-Costello, and E. Noble. Microvasc. Res. 49: 17-32, 1995] that severe disuse of rat extensor digitorum longus (EDL) muscle caused by a 2-wk application of tetrodotoxin (TTX) on the sciatic nerve is not accompanied by capillary loss. Using the same animal model, the present study examined whether this absence of coupling could be explained in terms of 1 ) too short a duration of disuse and 2 ) muscle-specific response to disuse. Fischer 344 rats were exposed to either no treatment (control) or to 2- or 8-wk TTX applications. Fiber size, capillary density per fiber cross-sectional area, and capillary-to-fiber (C/F) ratio were determined by morphometry in the EDL muscle (control, 2-   and 8-wk groups) and in the superficial portion of medial gastrocnemius (Gas) muscle (control, 2 wk). In both muscles, microvascular blood flow was evaluated by intravital microscopy [red blood cell velocity in capillaries ( V RBC )] and by laser Doppler flowmetry (LDF). Regardless of duration of TTX application or muscle type, TTX-induced disuse resulted in a significant reduction of fiber area (44-71%). However, capillary density increased in EDL muscle (both at 2 and 8 wk) but not in Gas muscle. C/F ratio decreased in EDL muscle at 8   wk (18%) and in Gas muscle (39%). This indicates that the effect on capillarity depended on duration of disuse and on muscle type. V RBC and LDF signal were significantly larger in EDL than in Gas muscle. Analysis of change in capillarity vs. V RBC suggested that the outcome of disuse may be modulated by blood flow. We conclude that the duration of skeletal muscle disuse per se does not dictate capillary loss, and we hypothesize that discrepant findings of coupling between functional demand and capillarity could be due to the presence/absence of flow-related angiogenesis superimposed on the capillary removal process during disuse. capillary density; blood flow; atrophy; capillary damage; angiogenesis</abstract><cop>Bethesda, MD</cop><pub>Am Physiological Soc</pub><pmid>10517784</pmid><doi>10.1152/jappl.1999.87.4.1496</doi><tpages>10</tpages></addata></record>
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source MEDLINE; American Physiological Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection
subjects Anatomy & physiology
Animals
Biological and medical sciences
Blood vessels
Blood Volume - drug effects
Body Weight - drug effects
Capillaries - pathology
Circulatory system
Fundamental and applied biological sciences. Psychology
Hemodynamics - drug effects
Hindlimb
Ischemia - physiopathology
Male
Microcirculation - drug effects
Microcirculation - physiology
Muscle, Skeletal - blood supply
Muscle, Skeletal - pathology
Muscle, Skeletal - physiology
Muscle, Skeletal - physiopathology
Muscular Atrophy - chemically induced
Muscular Atrophy - pathology
Muscular Atrophy - physiopathology
Muscular system
Rats
Rats, Inbred F344
Rodents
Sciatic Nerve - drug effects
Striated muscle. Tendons
Tetrodotoxin - pharmacology
Time Factors
Vertebrates: osteoarticular system, musculoskeletal system
title Differential microvascular response to disuse in rat hindlimb skeletal muscles
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