Induction of apoptosis in lung carcinoma cells by antiproliferative cyclic lipopeptides from marine algicolous isolate Bacillus atrophaeus strain AKLSR1
[Display omitted] •New surfactin group of cyclic lipopeptides isolated from B atrophaeus AKLSR1.•Isoforms exhibited structural variations in the peptide moiety and lipid chain length.•Cyclic lipopeptides showed good anticancer activity against different cancer cells.•Cyclic lipopeptides inhibited ca...
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| Veröffentlicht in: | Process biochemistry (1991) 2019-04, Vol.79, p.142-154 |
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| creator | Routhu, Sunitha Rani Nagarjuna Chary, R. Shaik, Anver Basha Prabhakar, S. Ganesh Kumar, C. Kamal, Ahmed |
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•New surfactin group of cyclic lipopeptides isolated from B atrophaeus AKLSR1.•Isoforms exhibited structural variations in the peptide moiety and lipid chain length.•Cyclic lipopeptides showed good anticancer activity against different cancer cells.•Cyclic lipopeptides inhibited cancer cell cycle progression in G0/G1 phase.•Cyclic lipopeptides induced apoptotic cell death via ROS accumulation in A549 cells.
Microbes possess a vast reservoir of bioactive compounds, including cyclic lipopeptides (CLPs). In the present study, an exopolymeric biosurfactant composed of lipoheptapeptides was isolated and identified from a marine algicolous bacterial isolate, Bacillus atrophaeus strain AKLSR1, endophytic to Padina tetrastromatica. The lipopeptides were characterized by using FT-IR, NMR, GC–MS and UPLC-ESI-Q-Tof-MS/MS spectroscopic studies. Structural analysis unveiled five CLP variants that formed one major polymeric lipopeptide. These cyclic lipoheptapeptides represent a new class of surfactin family, with significant variations in the fatty acid chain length and amino acid substitutions in the peptide moiety as compared to standard lipopeptides of surfactin family. The critical micelle concentration of this biosurfactant is 0.9 mg L−1 at a surface tension of 28 mN m−1. Polymeric biosurfactant showed stable emulsification index against various organic solvents and vegetable oils. CLPs showed substantial mortality of different cancer cell lines but no toxicity towards normal human lung cell line, MRC5. Further, mechanistic studies revealed cell cycle arrest, ROS accumulation, nuclear fragmentation and cell death. Our studies indicated that CLPs induced apoptosis in lung carcinoma cell line, A549. Anti-proliferative effect of CLPs produced by marine algicolous B. atrophaeus strain AKLSR1 suggests that it could be further explored for biomedical applications. |
| doi_str_mv | 10.1016/j.procbio.2018.12.010 |
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•New surfactin group of cyclic lipopeptides isolated from B atrophaeus AKLSR1.•Isoforms exhibited structural variations in the peptide moiety and lipid chain length.•Cyclic lipopeptides showed good anticancer activity against different cancer cells.•Cyclic lipopeptides inhibited cancer cell cycle progression in G0/G1 phase.•Cyclic lipopeptides induced apoptotic cell death via ROS accumulation in A549 cells.
Microbes possess a vast reservoir of bioactive compounds, including cyclic lipopeptides (CLPs). In the present study, an exopolymeric biosurfactant composed of lipoheptapeptides was isolated and identified from a marine algicolous bacterial isolate, Bacillus atrophaeus strain AKLSR1, endophytic to Padina tetrastromatica. The lipopeptides were characterized by using FT-IR, NMR, GC–MS and UPLC-ESI-Q-Tof-MS/MS spectroscopic studies. Structural analysis unveiled five CLP variants that formed one major polymeric lipopeptide. These cyclic lipoheptapeptides represent a new class of surfactin family, with significant variations in the fatty acid chain length and amino acid substitutions in the peptide moiety as compared to standard lipopeptides of surfactin family. The critical micelle concentration of this biosurfactant is 0.9 mg L−1 at a surface tension of 28 mN m−1. Polymeric biosurfactant showed stable emulsification index against various organic solvents and vegetable oils. CLPs showed substantial mortality of different cancer cell lines but no toxicity towards normal human lung cell line, MRC5. Further, mechanistic studies revealed cell cycle arrest, ROS accumulation, nuclear fragmentation and cell death. Our studies indicated that CLPs induced apoptosis in lung carcinoma cell line, A549. Anti-proliferative effect of CLPs produced by marine algicolous B. atrophaeus strain AKLSR1 suggests that it could be further explored for biomedical applications.</description><identifier>ISSN: 1359-5113</identifier><identifier>EISSN: 1873-3298</identifier><identifier>DOI: 10.1016/j.procbio.2018.12.010</identifier><language>eng</language><publisher>Barking: Elsevier Ltd</publisher><subject>Amino acids ; Anti-cancer ; Antiproliferatives ; Apoptosis ; Bacillus atrophaeus ; Bioactive compounds ; Biocompatibility ; Biomedical materials ; Biosurfactants ; Cell cycle ; Cell death ; DNA fragmentation ; Emulsification ; Endophytes ; Fatty acids ; Lipopeptides ; Lung carcinoma ; Micelles ; NMR ; Nuclear magnetic resonance ; Organic solvents ; Structural analysis ; Surface tension ; Surfactants ; Surfactin ; Toxicity ; Tumor cell lines ; Vegetable oils</subject><ispartof>Process biochemistry (1991), 2019-04, Vol.79, p.142-154</ispartof><rights>2018 Elsevier Ltd</rights><rights>Copyright Elsevier BV Apr 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c374t-ea385448d673bccc47f623069f210923e097c35e3e16e887c1f5b6459bee27e93</citedby><cites>FETCH-LOGICAL-c374t-ea385448d673bccc47f623069f210923e097c35e3e16e887c1f5b6459bee27e93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.procbio.2018.12.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids></links><search><creatorcontrib>Routhu, Sunitha Rani</creatorcontrib><creatorcontrib>Nagarjuna Chary, R.</creatorcontrib><creatorcontrib>Shaik, Anver Basha</creatorcontrib><creatorcontrib>Prabhakar, S.</creatorcontrib><creatorcontrib>Ganesh Kumar, C.</creatorcontrib><creatorcontrib>Kamal, Ahmed</creatorcontrib><title>Induction of apoptosis in lung carcinoma cells by antiproliferative cyclic lipopeptides from marine algicolous isolate Bacillus atrophaeus strain AKLSR1</title><title>Process biochemistry (1991)</title><description>[Display omitted]
•New surfactin group of cyclic lipopeptides isolated from B atrophaeus AKLSR1.•Isoforms exhibited structural variations in the peptide moiety and lipid chain length.•Cyclic lipopeptides showed good anticancer activity against different cancer cells.•Cyclic lipopeptides inhibited cancer cell cycle progression in G0/G1 phase.•Cyclic lipopeptides induced apoptotic cell death via ROS accumulation in A549 cells.
Microbes possess a vast reservoir of bioactive compounds, including cyclic lipopeptides (CLPs). In the present study, an exopolymeric biosurfactant composed of lipoheptapeptides was isolated and identified from a marine algicolous bacterial isolate, Bacillus atrophaeus strain AKLSR1, endophytic to Padina tetrastromatica. The lipopeptides were characterized by using FT-IR, NMR, GC–MS and UPLC-ESI-Q-Tof-MS/MS spectroscopic studies. Structural analysis unveiled five CLP variants that formed one major polymeric lipopeptide. These cyclic lipoheptapeptides represent a new class of surfactin family, with significant variations in the fatty acid chain length and amino acid substitutions in the peptide moiety as compared to standard lipopeptides of surfactin family. The critical micelle concentration of this biosurfactant is 0.9 mg L−1 at a surface tension of 28 mN m−1. Polymeric biosurfactant showed stable emulsification index against various organic solvents and vegetable oils. CLPs showed substantial mortality of different cancer cell lines but no toxicity towards normal human lung cell line, MRC5. Further, mechanistic studies revealed cell cycle arrest, ROS accumulation, nuclear fragmentation and cell death. Our studies indicated that CLPs induced apoptosis in lung carcinoma cell line, A549. Anti-proliferative effect of CLPs produced by marine algicolous B. atrophaeus strain AKLSR1 suggests that it could be further explored for biomedical applications.</description><subject>Amino acids</subject><subject>Anti-cancer</subject><subject>Antiproliferatives</subject><subject>Apoptosis</subject><subject>Bacillus atrophaeus</subject><subject>Bioactive compounds</subject><subject>Biocompatibility</subject><subject>Biomedical materials</subject><subject>Biosurfactants</subject><subject>Cell cycle</subject><subject>Cell death</subject><subject>DNA fragmentation</subject><subject>Emulsification</subject><subject>Endophytes</subject><subject>Fatty acids</subject><subject>Lipopeptides</subject><subject>Lung carcinoma</subject><subject>Micelles</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Organic solvents</subject><subject>Structural analysis</subject><subject>Surface tension</subject><subject>Surfactants</subject><subject>Surfactin</subject><subject>Toxicity</subject><subject>Tumor cell lines</subject><subject>Vegetable oils</subject><issn>1359-5113</issn><issn>1873-3298</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNqFUctq3TAQNaGFpEk_oSDo2q5G8nNV0tC0IRcCabsW8niczkXXciU5cP-kn1uFm31XcxjOg8Mpig8gK5DQftpXa_A4sq-UhL4CVUmQZ8UF9J0utRr6NxnrZigbAH1evItxL6UGAHlR_L1bpg0T-0X4WdjVr8lHjoIX4bblSaANyIs_WIHkXBTjUdglcQ50PFOwiZ9J4BEdo3Cc5bQmniiKOfiDONjACwnrnhi981v2jd7ZROKLRXYuP2wKfv1tKcOYgs251_e7H49wVbydrYv0_vVeFr9uv_68-V7uHr7d3VzvStRdnUqyum_qup_aTo-IWHdzq7Rsh1mBHJQmOXSoG9IELfV9hzA3Y1s3w0ikOhr0ZfHx5Jsr_dkoJrP3W1hypFFKya4ZoIXMak4sDD7GQLNZA-d2RwPSvIxg9uZ1BPMyggFl8ghZ9_mko1zhmSmYiEwL0sSBMJnJ838c_gHFKJYv</recordid><startdate>20190401</startdate><enddate>20190401</enddate><creator>Routhu, Sunitha Rani</creator><creator>Nagarjuna Chary, R.</creator><creator>Shaik, Anver Basha</creator><creator>Prabhakar, S.</creator><creator>Ganesh Kumar, C.</creator><creator>Kamal, Ahmed</creator><general>Elsevier Ltd</general><general>Elsevier BV</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20190401</creationdate><title>Induction of apoptosis in lung carcinoma cells by antiproliferative cyclic lipopeptides from marine algicolous isolate Bacillus atrophaeus strain AKLSR1</title><author>Routhu, Sunitha Rani ; Nagarjuna Chary, R. ; Shaik, Anver Basha ; Prabhakar, S. ; Ganesh Kumar, C. ; Kamal, Ahmed</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c374t-ea385448d673bccc47f623069f210923e097c35e3e16e887c1f5b6459bee27e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Amino acids</topic><topic>Anti-cancer</topic><topic>Antiproliferatives</topic><topic>Apoptosis</topic><topic>Bacillus atrophaeus</topic><topic>Bioactive compounds</topic><topic>Biocompatibility</topic><topic>Biomedical materials</topic><topic>Biosurfactants</topic><topic>Cell cycle</topic><topic>Cell death</topic><topic>DNA fragmentation</topic><topic>Emulsification</topic><topic>Endophytes</topic><topic>Fatty acids</topic><topic>Lipopeptides</topic><topic>Lung carcinoma</topic><topic>Micelles</topic><topic>NMR</topic><topic>Nuclear magnetic resonance</topic><topic>Organic solvents</topic><topic>Structural analysis</topic><topic>Surface tension</topic><topic>Surfactants</topic><topic>Surfactin</topic><topic>Toxicity</topic><topic>Tumor cell lines</topic><topic>Vegetable oils</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Routhu, Sunitha Rani</creatorcontrib><creatorcontrib>Nagarjuna Chary, R.</creatorcontrib><creatorcontrib>Shaik, Anver Basha</creatorcontrib><creatorcontrib>Prabhakar, S.</creatorcontrib><creatorcontrib>Ganesh Kumar, C.</creatorcontrib><creatorcontrib>Kamal, Ahmed</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Process biochemistry (1991)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Routhu, Sunitha Rani</au><au>Nagarjuna Chary, R.</au><au>Shaik, Anver Basha</au><au>Prabhakar, S.</au><au>Ganesh Kumar, C.</au><au>Kamal, Ahmed</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of apoptosis in lung carcinoma cells by antiproliferative cyclic lipopeptides from marine algicolous isolate Bacillus atrophaeus strain AKLSR1</atitle><jtitle>Process biochemistry (1991)</jtitle><date>2019-04-01</date><risdate>2019</risdate><volume>79</volume><spage>142</spage><epage>154</epage><pages>142-154</pages><issn>1359-5113</issn><eissn>1873-3298</eissn><abstract>[Display omitted]
•New surfactin group of cyclic lipopeptides isolated from B atrophaeus AKLSR1.•Isoforms exhibited structural variations in the peptide moiety and lipid chain length.•Cyclic lipopeptides showed good anticancer activity against different cancer cells.•Cyclic lipopeptides inhibited cancer cell cycle progression in G0/G1 phase.•Cyclic lipopeptides induced apoptotic cell death via ROS accumulation in A549 cells.
Microbes possess a vast reservoir of bioactive compounds, including cyclic lipopeptides (CLPs). In the present study, an exopolymeric biosurfactant composed of lipoheptapeptides was isolated and identified from a marine algicolous bacterial isolate, Bacillus atrophaeus strain AKLSR1, endophytic to Padina tetrastromatica. The lipopeptides were characterized by using FT-IR, NMR, GC–MS and UPLC-ESI-Q-Tof-MS/MS spectroscopic studies. Structural analysis unveiled five CLP variants that formed one major polymeric lipopeptide. These cyclic lipoheptapeptides represent a new class of surfactin family, with significant variations in the fatty acid chain length and amino acid substitutions in the peptide moiety as compared to standard lipopeptides of surfactin family. The critical micelle concentration of this biosurfactant is 0.9 mg L−1 at a surface tension of 28 mN m−1. Polymeric biosurfactant showed stable emulsification index against various organic solvents and vegetable oils. CLPs showed substantial mortality of different cancer cell lines but no toxicity towards normal human lung cell line, MRC5. Further, mechanistic studies revealed cell cycle arrest, ROS accumulation, nuclear fragmentation and cell death. Our studies indicated that CLPs induced apoptosis in lung carcinoma cell line, A549. Anti-proliferative effect of CLPs produced by marine algicolous B. atrophaeus strain AKLSR1 suggests that it could be further explored for biomedical applications.</abstract><cop>Barking</cop><pub>Elsevier Ltd</pub><doi>10.1016/j.procbio.2018.12.010</doi><tpages>13</tpages></addata></record> |
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| subjects | Amino acids Anti-cancer Antiproliferatives Apoptosis Bacillus atrophaeus Bioactive compounds Biocompatibility Biomedical materials Biosurfactants Cell cycle Cell death DNA fragmentation Emulsification Endophytes Fatty acids Lipopeptides Lung carcinoma Micelles NMR Nuclear magnetic resonance Organic solvents Structural analysis Surface tension Surfactants Surfactin Toxicity Tumor cell lines Vegetable oils |
| title | Induction of apoptosis in lung carcinoma cells by antiproliferative cyclic lipopeptides from marine algicolous isolate Bacillus atrophaeus strain AKLSR1 |
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