Production of a novel glycerol-inducible lipase from thermophilic Geobacillus stearothermophilus strain-5
In a screening program for isolation of thermophilic lipase-producing bacteria, a number of thermophilic bacteria were isolated from desert soil from Baltim, Egypt. Among 55 isolates, a potent bacterial candidate (starin-5) was characterized and identified by biochemical and PCR techniques, based on...
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| Veröffentlicht in: | World journal of microbiology & biotechnology 2009-02, Vol.25 (2), p.287-294 |
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| creator | Berekaa, Mahmoud M Zaghloul, Taha I Abdel-Fattah, Yasser R Saeed, Hesham M Sifour, Mohamed |
| description | In a screening program for isolation of thermophilic lipase-producing bacteria, a number of thermophilic bacteria were isolated from desert soil from Baltim, Egypt. Among 55 isolates, a potent bacterial candidate (starin-5) was characterized and identified by biochemical and PCR techniques, based on 16S rRNA sequencing. Phylogenetic analysis revealed its closeness to geobacilli especially the thermophilic Geobacillus stearothermophilus with optimal growth and lipolytic enzyme activity at 60°C and pH 7.0. An inducible nature of lipolytic enzyme synthesis using glycerol and glucose was demonstrated. Approximately, 94-100% of the original activity was retained due to thermal stability of the crude enzyme after heat treatment for 15 min at 30-60°C. The enzyme retained 84.84% of its original activity during incubation at 70°C (pH 8.0) for 15 min. Lipase enzyme from G. stearothermophilus strain-5 was immobilized on various carriers and the most suitable carrier was chitin that showed 73.03% of activity yield. |
| doi_str_mv | 10.1007/s11274-008-9891-3 |
| format | Article |
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Among 55 isolates, a potent bacterial candidate (starin-5) was characterized and identified by biochemical and PCR techniques, based on 16S rRNA sequencing. Phylogenetic analysis revealed its closeness to geobacilli especially the thermophilic Geobacillus stearothermophilus with optimal growth and lipolytic enzyme activity at 60°C and pH 7.0. An inducible nature of lipolytic enzyme synthesis using glycerol and glucose was demonstrated. Approximately, 94-100% of the original activity was retained due to thermal stability of the crude enzyme after heat treatment for 15 min at 30-60°C. The enzyme retained 84.84% of its original activity during incubation at 70°C (pH 8.0) for 15 min. Lipase enzyme from G. stearothermophilus strain-5 was immobilized on various carriers and the most suitable carrier was chitin that showed 73.03% of activity yield.</description><identifier>ISSN: 0959-3993</identifier><identifier>EISSN: 1573-0972</identifier><identifier>DOI: 10.1007/s11274-008-9891-3</identifier><language>eng</language><publisher>Dordrecht: Dordrecht : Springer Netherlands</publisher><subject>Applied Microbiology ; Bacteria ; Biochemistry ; Biological and medical sciences ; Biomedical and Life Sciences ; Biotechnology ; Chitin ; Desert soils ; Environmental Engineering/Biotechnology ; Enzymatic activity ; Enzymes ; Fundamental and applied biological sciences. Psychology ; General aspects ; Glucose ; Glycerol ; Heat treatment ; Immobilization techniques ; Investigations ; Life Sciences ; Methods. Procedures. 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Among 55 isolates, a potent bacterial candidate (starin-5) was characterized and identified by biochemical and PCR techniques, based on 16S rRNA sequencing. Phylogenetic analysis revealed its closeness to geobacilli especially the thermophilic Geobacillus stearothermophilus with optimal growth and lipolytic enzyme activity at 60°C and pH 7.0. An inducible nature of lipolytic enzyme synthesis using glycerol and glucose was demonstrated. Approximately, 94-100% of the original activity was retained due to thermal stability of the crude enzyme after heat treatment for 15 min at 30-60°C. The enzyme retained 84.84% of its original activity during incubation at 70°C (pH 8.0) for 15 min. Lipase enzyme from G. stearothermophilus strain-5 was immobilized on various carriers and the most suitable carrier was chitin that showed 73.03% of activity yield.</description><subject>Applied Microbiology</subject><subject>Bacteria</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Chitin</subject><subject>Desert soils</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Enzymatic activity</subject><subject>Enzymes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Glucose</subject><subject>Glycerol</subject><subject>Heat treatment</subject><subject>Immobilization techniques</subject><subject>Investigations</subject><subject>Life Sciences</subject><subject>Methods. Procedures. 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lipase-producing bacteria, a number of thermophilic bacteria were isolated from desert soil from Baltim, Egypt. Among 55 isolates, a potent bacterial candidate (starin-5) was characterized and identified by biochemical and PCR techniques, based on 16S rRNA sequencing. Phylogenetic analysis revealed its closeness to geobacilli especially the thermophilic Geobacillus stearothermophilus with optimal growth and lipolytic enzyme activity at 60°C and pH 7.0. An inducible nature of lipolytic enzyme synthesis using glycerol and glucose was demonstrated. Approximately, 94-100% of the original activity was retained due to thermal stability of the crude enzyme after heat treatment for 15 min at 30-60°C. The enzyme retained 84.84% of its original activity during incubation at 70°C (pH 8.0) for 15 min. Lipase enzyme from G. stearothermophilus strain-5 was immobilized on various carriers and the most suitable carrier was chitin that showed 73.03% of activity yield.</abstract><cop>Dordrecht</cop><pub>Dordrecht : Springer Netherlands</pub><doi>10.1007/s11274-008-9891-3</doi><tpages>8</tpages></addata></record> |
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| ispartof | World journal of microbiology & biotechnology, 2009-02, Vol.25 (2), p.287-294 |
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| subjects | Applied Microbiology Bacteria Biochemistry Biological and medical sciences Biomedical and Life Sciences Biotechnology Chitin Desert soils Environmental Engineering/Biotechnology Enzymatic activity Enzymes Fundamental and applied biological sciences. Psychology General aspects Glucose Glycerol Heat treatment Immobilization techniques Investigations Life Sciences Methods. Procedures. Technologies Microbiology Olive oil Original Paper Solvents Studies |
| title | Production of a novel glycerol-inducible lipase from thermophilic Geobacillus stearothermophilus strain-5 |
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