Detecting the undetectable: Characterization, optimization, and validation of an eDNA detection assay for the federally endangered dwarf wedgemussel, Alasmidonta heterodon (Bivalvia: Unionoida)

Environmental DNA (eDNA) assays are valuable tools for monitoring the presence and distribution of cryptic species. Like many freshwater mussels, the numbers of dwarf wedgemussel, Alasmidonta heterodon, have dwindled and its range has diminished. As of its listing in 1993, only 10–20 locations were...

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Veröffentlicht in:	Aquatic conservation 2019-04, Vol.29 (4), p.603-611
Hauptverfasser:	Schill, William B., Galbraith, Heather S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Alasmidonta heterodon Assaying Bacteria Binding sites Biodiversity Cryptic species Cytochrome Cytochrome-c oxidase Cytochromes Deoxyribonucleic acid Detection DNA DNA probes DNA viruses endangered species Environmental DNA Flocculation Freshwater Freshwater molluscs Historical account Inland water environment invertebrates Mussels new techniques Nucleotide sequence Nucleotides Optimization Pathogens PCR Phylogenetics Phylogeny Polymerase chain reaction Polymorphism Populations Primers river Single-nucleotide polymorphism stream Viruses
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