Purification-independent immunoreagents obtained by displaying nanobodies on bacteria surface

The availability of preimmune libraries of antibody fragments allows for the fast generation of binders which can be expressed in both eukaryotic and prokaryotic systems. We exploited the recombinant nature of antibody fragments to demonstrate the possibility of expressing them as functional protein...

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Veröffentlicht in:	Applied microbiology and biotechnology 2019-06, Vol.103 (11), p.4443-4453
Hauptverfasser:	Oloketuyi, Sandra, Dilkaute, Carina, Mazzega, Elisa, Jose, Joachim, de Marco, Ario
Format:	Artikel
Sprache:	eng
Schlagworte:	Algae Antibiotics Antibodies Aquatic microorganisms Bacteria Binders Biomedical and Life Sciences Biotechnologically Relevant Enzymes and Proteins Biotechnology Cancer Cytoplasm Dehydrogenases Diagnostic software Diagnostic systems E coli Escherichia coli Flow cytometry Fluorescence Fragments Life Sciences Microbial Genetics and Genomics Microbiology Nanobodies Nanotechnology Observations Plasmids Proteins Purification Reagents Technology application
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