Establishment of a novel patient-derived Ewing's sarcoma cell line, NCC-ES1-C1
Ewing's sarcoma is an aggressive mesenchymal tumor characterized by the presence of a unique EWSR1-FLI1 translocation. Ewing's sarcoma primarily occurs in the bone and soft tissues. Cell lines enable researchers to investigate the molecular backgrounds of disease and the significance of ge...
Gespeichert in:
| Veröffentlicht in: | In vitro cellular & developmental biology. Animal 2018-12, Vol.54 (10), p.770-778 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 778 |
|---|---|
| container_issue | 10 |
| container_start_page | 770 |
| container_title | In vitro cellular & developmental biology. Animal |
| container_volume | 54 |
| creator | Oyama, Rieko Kito, Fusako Qiao, Zhiwei Sakumoto, Marimu Noguchi, Rei Takahashi, Mami Toki, Shunichi Tanzawa, Yoshikazu Yoshida, Akihiko Kawai, Akira Kondo, Tadashi |
| description | Ewing's sarcoma is an aggressive mesenchymal tumor characterized by the presence of a unique EWSR1-FLI1 translocation. Ewing's sarcoma primarily occurs in the bone and soft tissues. Cell lines enable researchers to investigate the molecular backgrounds of disease and the significance of genetic alterations in relevant cellular contexts. Here, we report the establishment and characterization of a novel Ewing's sarcoma cell line following primary Ewing's sarcoma tumor tissue culture. The established cell line was authenticated by DNA microsatellite short tandem repeat analysis, characterized by in vitro assays, and named NCC-ES1-C1. The NCC-ES1-C1 cell line grew well for 15 mo and was subcultured more than 50 times during this period. Characterization of the cells revealed that they were not adherent and showed floating features. In conclusion, we successfully established a novel Ewing's sarcoma cell line, NCC-ES1-C1, from primary tumor tissue. The cell line has the characteristic EWSR1-FLI1 gene fusion and exhibits aggressive growth in vitro. Thus, the NCC-ES1-C1 cell line will be a useful tool for investigating the mechanisms of disease and the biological role of the EWSR1-FLI1 fusion gene. |
| doi_str_mv | 10.1007/s11626-018-0302-2 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_journals_2207813541</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>45179013</jstor_id><sourcerecordid>45179013</sourcerecordid><originalsourceid>FETCH-LOGICAL-c468t-47797da8b30d9639d7bce9f23769e135ec452480ccb43bfbf72b8c5f8c0cf15a3</originalsourceid><addsrcrecordid>eNp9kMtKxDAUhoMo3h_AjQRE3Bg9J0mbdillvICMCxXchTRNtUOnHZOO4tubod5WZpNw8l8OHyEHCGcIoM4DYspTBpgxEMAZXyPbmEjBFKRP6_ENChlPc9giOyHMIJ4c002yJUBwyaXcJtNJGEzZNuFl7rqB9jU1tOvfXEsXZmjiiFXON2-uopP3pns-CTQYb_u5oda1LW2bzp3SaVGwyT2yAvfIRm3a4Pa_7l3yeDl5KK7Z7d3VTXFxy6xMs4FJpXJVmawUUOWpyCtVWpfXXKg0dygSZ2XCZQbWllKUdVkrXmY2qTMLtsbEiF1yNOYufP-6dGHQs37pu1ipOQeVxQyJUYWjyvo-BO9qvfDN3PgPjaBXBPVIUEeCekVQ8-g5_EpelnNX_Ti-kUUBHwUhfnXPzv9W_5d6PJpmYej93zW4iAaZoMoBhfgErjqEzQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2207813541</pqid></control><display><type>article</type><title>Establishment of a novel patient-derived Ewing's sarcoma cell line, NCC-ES1-C1</title><source>MEDLINE</source><source>Jstor Complete Legacy</source><source>Springer Nature - Complete Springer Journals</source><creator>Oyama, Rieko ; Kito, Fusako ; Qiao, Zhiwei ; Sakumoto, Marimu ; Noguchi, Rei ; Takahashi, Mami ; Toki, Shunichi ; Tanzawa, Yoshikazu ; Yoshida, Akihiko ; Kawai, Akira ; Kondo, Tadashi</creator><creatorcontrib>Oyama, Rieko ; Kito, Fusako ; Qiao, Zhiwei ; Sakumoto, Marimu ; Noguchi, Rei ; Takahashi, Mami ; Toki, Shunichi ; Tanzawa, Yoshikazu ; Yoshida, Akihiko ; Kawai, Akira ; Kondo, Tadashi</creatorcontrib><description>Ewing's sarcoma is an aggressive mesenchymal tumor characterized by the presence of a unique EWSR1-FLI1 translocation. Ewing's sarcoma primarily occurs in the bone and soft tissues. Cell lines enable researchers to investigate the molecular backgrounds of disease and the significance of genetic alterations in relevant cellular contexts. Here, we report the establishment and characterization of a novel Ewing's sarcoma cell line following primary Ewing's sarcoma tumor tissue culture. The established cell line was authenticated by DNA microsatellite short tandem repeat analysis, characterized by in vitro assays, and named NCC-ES1-C1. The NCC-ES1-C1 cell line grew well for 15 mo and was subcultured more than 50 times during this period. Characterization of the cells revealed that they were not adherent and showed floating features. In conclusion, we successfully established a novel Ewing's sarcoma cell line, NCC-ES1-C1, from primary tumor tissue. The cell line has the characteristic EWSR1-FLI1 gene fusion and exhibits aggressive growth in vitro. Thus, the NCC-ES1-C1 cell line will be a useful tool for investigating the mechanisms of disease and the biological role of the EWSR1-FLI1 fusion gene.</description><identifier>ISSN: 1071-2690</identifier><identifier>EISSN: 1543-706X</identifier><identifier>DOI: 10.1007/s11626-018-0302-2</identifier><identifier>PMID: 30324244</identifier><language>eng</language><publisher>New York: Springer Science & Business Media LLC</publisher><subject>Adult ; Animal Genetics and Genomics ; Animals ; Antineoplastic Agents - pharmacology ; Base Sequence ; Biomedical and Life Sciences ; Cancer therapies ; Cell Biology ; Cell Culture ; Cell Line, Tumor ; Chemotherapy ; Children & youth ; Deoxyribonucleic acid ; Developmental Biology ; DNA ; Drug Evaluation, Preclinical ; Drugs ; ESTABLISHMENT OF CELL LINES ; Ewing's sarcoma ; Ewings sarcoma ; Female ; FLI-1 protein ; FLI1 protein ; Fusion protein ; Gene Dosage ; Gene expression ; Gene Expression Regulation, Neoplastic - drug effects ; Genetic testing ; Humans ; Kinases ; Life Sciences ; Male ; Medical prognosis ; Mesenchyme ; Metastasis ; Mice, Inbred NOD ; Microsatellite Repeats - genetics ; Oncogene Proteins, Fusion - genetics ; Oncogene Proteins, Fusion - metabolism ; Patients ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Sarcoma ; Sarcoma, Ewing - diagnostic imaging ; Sarcoma, Ewing - genetics ; Sarcoma, Ewing - pathology ; Soft tissues ; Stem Cells ; Tissue culture ; Tissues ; Translocation ; Tumors ; Xenograft Model Antitumor Assays</subject><ispartof>In vitro cellular & developmental biology. Animal, 2018-12, Vol.54 (10), p.770-778</ispartof><rights>2018 Society for In Vitro Biology</rights><rights>The Society for In Vitro Biology 2018</rights><rights>Copyright Society for In Vitro Biology Dec 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c468t-47797da8b30d9639d7bce9f23769e135ec452480ccb43bfbf72b8c5f8c0cf15a3</citedby><cites>FETCH-LOGICAL-c468t-47797da8b30d9639d7bce9f23769e135ec452480ccb43bfbf72b8c5f8c0cf15a3</cites><orcidid>0000-0001-6405-7792</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/45179013$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/45179013$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,777,781,800,27905,27906,41469,42538,51300,57998,58231</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30324244$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oyama, Rieko</creatorcontrib><creatorcontrib>Kito, Fusako</creatorcontrib><creatorcontrib>Qiao, Zhiwei</creatorcontrib><creatorcontrib>Sakumoto, Marimu</creatorcontrib><creatorcontrib>Noguchi, Rei</creatorcontrib><creatorcontrib>Takahashi, Mami</creatorcontrib><creatorcontrib>Toki, Shunichi</creatorcontrib><creatorcontrib>Tanzawa, Yoshikazu</creatorcontrib><creatorcontrib>Yoshida, Akihiko</creatorcontrib><creatorcontrib>Kawai, Akira</creatorcontrib><creatorcontrib>Kondo, Tadashi</creatorcontrib><title>Establishment of a novel patient-derived Ewing's sarcoma cell line, NCC-ES1-C1</title><title>In vitro cellular & developmental biology. Animal</title><addtitle>In Vitro Cell.Dev.Biol.-Animal</addtitle><addtitle>In Vitro Cell Dev Biol Anim</addtitle><description>Ewing's sarcoma is an aggressive mesenchymal tumor characterized by the presence of a unique EWSR1-FLI1 translocation. Ewing's sarcoma primarily occurs in the bone and soft tissues. Cell lines enable researchers to investigate the molecular backgrounds of disease and the significance of genetic alterations in relevant cellular contexts. Here, we report the establishment and characterization of a novel Ewing's sarcoma cell line following primary Ewing's sarcoma tumor tissue culture. The established cell line was authenticated by DNA microsatellite short tandem repeat analysis, characterized by in vitro assays, and named NCC-ES1-C1. The NCC-ES1-C1 cell line grew well for 15 mo and was subcultured more than 50 times during this period. Characterization of the cells revealed that they were not adherent and showed floating features. In conclusion, we successfully established a novel Ewing's sarcoma cell line, NCC-ES1-C1, from primary tumor tissue. The cell line has the characteristic EWSR1-FLI1 gene fusion and exhibits aggressive growth in vitro. Thus, the NCC-ES1-C1 cell line will be a useful tool for investigating the mechanisms of disease and the biological role of the EWSR1-FLI1 fusion gene.</description><subject>Adult</subject><subject>Animal Genetics and Genomics</subject><subject>Animals</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Base Sequence</subject><subject>Biomedical and Life Sciences</subject><subject>Cancer therapies</subject><subject>Cell Biology</subject><subject>Cell Culture</subject><subject>Cell Line, Tumor</subject><subject>Chemotherapy</subject><subject>Children & youth</subject><subject>Deoxyribonucleic acid</subject><subject>Developmental Biology</subject><subject>DNA</subject><subject>Drug Evaluation, Preclinical</subject><subject>Drugs</subject><subject>ESTABLISHMENT OF CELL LINES</subject><subject>Ewing's sarcoma</subject><subject>Ewings sarcoma</subject><subject>Female</subject><subject>FLI-1 protein</subject><subject>FLI1 protein</subject><subject>Fusion protein</subject><subject>Gene Dosage</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Neoplastic - drug effects</subject><subject>Genetic testing</subject><subject>Humans</subject><subject>Kinases</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Medical prognosis</subject><subject>Mesenchyme</subject><subject>Metastasis</subject><subject>Mice, Inbred NOD</subject><subject>Microsatellite Repeats - genetics</subject><subject>Oncogene Proteins, Fusion - genetics</subject><subject>Oncogene Proteins, Fusion - metabolism</subject><subject>Patients</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sarcoma</subject><subject>Sarcoma, Ewing - diagnostic imaging</subject><subject>Sarcoma, Ewing - genetics</subject><subject>Sarcoma, Ewing - pathology</subject><subject>Soft tissues</subject><subject>Stem Cells</subject><subject>Tissue culture</subject><subject>Tissues</subject><subject>Translocation</subject><subject>Tumors</subject><subject>Xenograft Model Antitumor Assays</subject><issn>1071-2690</issn><issn>1543-706X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kMtKxDAUhoMo3h_AjQRE3Bg9J0mbdillvICMCxXchTRNtUOnHZOO4tubod5WZpNw8l8OHyEHCGcIoM4DYspTBpgxEMAZXyPbmEjBFKRP6_ENChlPc9giOyHMIJ4c002yJUBwyaXcJtNJGEzZNuFl7rqB9jU1tOvfXEsXZmjiiFXON2-uopP3pns-CTQYb_u5oda1LW2bzp3SaVGwyT2yAvfIRm3a4Pa_7l3yeDl5KK7Z7d3VTXFxy6xMs4FJpXJVmawUUOWpyCtVWpfXXKg0dygSZ2XCZQbWllKUdVkrXmY2qTMLtsbEiF1yNOYufP-6dGHQs37pu1ipOQeVxQyJUYWjyvo-BO9qvfDN3PgPjaBXBPVIUEeCekVQ8-g5_EpelnNX_Ti-kUUBHwUhfnXPzv9W_5d6PJpmYej93zW4iAaZoMoBhfgErjqEzQ</recordid><startdate>20181201</startdate><enddate>20181201</enddate><creator>Oyama, Rieko</creator><creator>Kito, Fusako</creator><creator>Qiao, Zhiwei</creator><creator>Sakumoto, Marimu</creator><creator>Noguchi, Rei</creator><creator>Takahashi, Mami</creator><creator>Toki, Shunichi</creator><creator>Tanzawa, Yoshikazu</creator><creator>Yoshida, Akihiko</creator><creator>Kawai, Akira</creator><creator>Kondo, Tadashi</creator><general>Springer Science & Business Media LLC</general><general>Springer US</general><general>Society for In Vitro Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>S0X</scope><orcidid>https://orcid.org/0000-0001-6405-7792</orcidid></search><sort><creationdate>20181201</creationdate><title>Establishment of a novel patient-derived Ewing's sarcoma cell line, NCC-ES1-C1</title><author>Oyama, Rieko ; Kito, Fusako ; Qiao, Zhiwei ; Sakumoto, Marimu ; Noguchi, Rei ; Takahashi, Mami ; Toki, Shunichi ; Tanzawa, Yoshikazu ; Yoshida, Akihiko ; Kawai, Akira ; Kondo, Tadashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c468t-47797da8b30d9639d7bce9f23769e135ec452480ccb43bfbf72b8c5f8c0cf15a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adult</topic><topic>Animal Genetics and Genomics</topic><topic>Animals</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Base Sequence</topic><topic>Biomedical and Life Sciences</topic><topic>Cancer therapies</topic><topic>Cell Biology</topic><topic>Cell Culture</topic><topic>Cell Line, Tumor</topic><topic>Chemotherapy</topic><topic>Children & youth</topic><topic>Deoxyribonucleic acid</topic><topic>Developmental Biology</topic><topic>DNA</topic><topic>Drug Evaluation, Preclinical</topic><topic>Drugs</topic><topic>ESTABLISHMENT OF CELL LINES</topic><topic>Ewing's sarcoma</topic><topic>Ewings sarcoma</topic><topic>Female</topic><topic>FLI-1 protein</topic><topic>FLI1 protein</topic><topic>Fusion protein</topic><topic>Gene Dosage</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Neoplastic - drug effects</topic><topic>Genetic testing</topic><topic>Humans</topic><topic>Kinases</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Medical prognosis</topic><topic>Mesenchyme</topic><topic>Metastasis</topic><topic>Mice, Inbred NOD</topic><topic>Microsatellite Repeats - genetics</topic><topic>Oncogene Proteins, Fusion - genetics</topic><topic>Oncogene Proteins, Fusion - metabolism</topic><topic>Patients</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Sarcoma</topic><topic>Sarcoma, Ewing - diagnostic imaging</topic><topic>Sarcoma, Ewing - genetics</topic><topic>Sarcoma, Ewing - pathology</topic><topic>Soft tissues</topic><topic>Stem Cells</topic><topic>Tissue culture</topic><topic>Tissues</topic><topic>Translocation</topic><topic>Tumors</topic><topic>Xenograft Model Antitumor Assays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oyama, Rieko</creatorcontrib><creatorcontrib>Kito, Fusako</creatorcontrib><creatorcontrib>Qiao, Zhiwei</creatorcontrib><creatorcontrib>Sakumoto, Marimu</creatorcontrib><creatorcontrib>Noguchi, Rei</creatorcontrib><creatorcontrib>Takahashi, Mami</creatorcontrib><creatorcontrib>Toki, Shunichi</creatorcontrib><creatorcontrib>Tanzawa, Yoshikazu</creatorcontrib><creatorcontrib>Yoshida, Akihiko</creatorcontrib><creatorcontrib>Kawai, Akira</creatorcontrib><creatorcontrib>Kondo, Tadashi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><jtitle>In vitro cellular & developmental biology. Animal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oyama, Rieko</au><au>Kito, Fusako</au><au>Qiao, Zhiwei</au><au>Sakumoto, Marimu</au><au>Noguchi, Rei</au><au>Takahashi, Mami</au><au>Toki, Shunichi</au><au>Tanzawa, Yoshikazu</au><au>Yoshida, Akihiko</au><au>Kawai, Akira</au><au>Kondo, Tadashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment of a novel patient-derived Ewing's sarcoma cell line, NCC-ES1-C1</atitle><jtitle>In vitro cellular & developmental biology. Animal</jtitle><stitle>In Vitro Cell.Dev.Biol.-Animal</stitle><addtitle>In Vitro Cell Dev Biol Anim</addtitle><date>2018-12-01</date><risdate>2018</risdate><volume>54</volume><issue>10</issue><spage>770</spage><epage>778</epage><pages>770-778</pages><issn>1071-2690</issn><eissn>1543-706X</eissn><abstract>Ewing's sarcoma is an aggressive mesenchymal tumor characterized by the presence of a unique EWSR1-FLI1 translocation. Ewing's sarcoma primarily occurs in the bone and soft tissues. Cell lines enable researchers to investigate the molecular backgrounds of disease and the significance of genetic alterations in relevant cellular contexts. Here, we report the establishment and characterization of a novel Ewing's sarcoma cell line following primary Ewing's sarcoma tumor tissue culture. The established cell line was authenticated by DNA microsatellite short tandem repeat analysis, characterized by in vitro assays, and named NCC-ES1-C1. The NCC-ES1-C1 cell line grew well for 15 mo and was subcultured more than 50 times during this period. Characterization of the cells revealed that they were not adherent and showed floating features. In conclusion, we successfully established a novel Ewing's sarcoma cell line, NCC-ES1-C1, from primary tumor tissue. The cell line has the characteristic EWSR1-FLI1 gene fusion and exhibits aggressive growth in vitro. Thus, the NCC-ES1-C1 cell line will be a useful tool for investigating the mechanisms of disease and the biological role of the EWSR1-FLI1 fusion gene.</abstract><cop>New York</cop><pub>Springer Science & Business Media LLC</pub><pmid>30324244</pmid><doi>10.1007/s11626-018-0302-2</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-6405-7792</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1071-2690 |
| ispartof | In vitro cellular & developmental biology. Animal, 2018-12, Vol.54 (10), p.770-778 |
| issn | 1071-2690 1543-706X |
| language | eng |
| recordid | cdi_proquest_journals_2207813541 |
| source | MEDLINE; Jstor Complete Legacy; Springer Nature - Complete Springer Journals |
| subjects | Adult Animal Genetics and Genomics Animals Antineoplastic Agents - pharmacology Base Sequence Biomedical and Life Sciences Cancer therapies Cell Biology Cell Culture Cell Line, Tumor Chemotherapy Children & youth Deoxyribonucleic acid Developmental Biology DNA Drug Evaluation, Preclinical Drugs ESTABLISHMENT OF CELL LINES Ewing's sarcoma Ewings sarcoma Female FLI-1 protein FLI1 protein Fusion protein Gene Dosage Gene expression Gene Expression Regulation, Neoplastic - drug effects Genetic testing Humans Kinases Life Sciences Male Medical prognosis Mesenchyme Metastasis Mice, Inbred NOD Microsatellite Repeats - genetics Oncogene Proteins, Fusion - genetics Oncogene Proteins, Fusion - metabolism Patients RNA, Messenger - genetics RNA, Messenger - metabolism Sarcoma Sarcoma, Ewing - diagnostic imaging Sarcoma, Ewing - genetics Sarcoma, Ewing - pathology Soft tissues Stem Cells Tissue culture Tissues Translocation Tumors Xenograft Model Antitumor Assays |
| title | Establishment of a novel patient-derived Ewing's sarcoma cell line, NCC-ES1-C1 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T14%3A34%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Establishment%20of%20a%20novel%20patient-derived%20Ewing's%20sarcoma%20cell%20line,%20NCC-ES1-C1&rft.jtitle=In%20vitro%20cellular%20&%20developmental%20biology.%20Animal&rft.au=Oyama,%20Rieko&rft.date=2018-12-01&rft.volume=54&rft.issue=10&rft.spage=770&rft.epage=778&rft.pages=770-778&rft.issn=1071-2690&rft.eissn=1543-706X&rft_id=info:doi/10.1007/s11626-018-0302-2&rft_dat=%3Cjstor_proqu%3E45179013%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2207813541&rft_id=info:pmid/30324244&rft_jstor_id=45179013&rfr_iscdi=true |