Characterization of cell lines derived from the southern armyworm, Spodoptera eridania
Spodoptera eridania (southern armyworm) is a polyphagous pest of many plants, including field crops, vegetables, fruits, and ornamentals. Larvae are leaf feeders, defoliating many crops in the tropics and subtropics of the western hemisphere. In this study, cell lines from S. eridania were establish...
Gespeichert in:
| Veröffentlicht in: | In vitro cellular & developmental biology. Animal 2018-12, Vol.54 (10), p.749-755 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 755 |
|---|---|
| container_issue | 10 |
| container_start_page | 749 |
| container_title | In vitro cellular & developmental biology. Animal |
| container_volume | 54 |
| creator | Zhang, Hongwei Li, Yaofa Reall, Tamra Xu, Yiyun Goodman, Cynthia Saathoff, Stephen Ringbauer, Joseph Stanley, David |
| description | Spodoptera eridania (southern armyworm) is a polyphagous pest of many plants, including field crops, vegetables, fruits, and ornamentals. Larvae are leaf feeders, defoliating many crops in the tropics and subtropics of the western hemisphere. In this study, cell lines from S. eridania were established to support research focused on the development of advanced pest management technologies. We generated seven cell lines from larval tissues: three from nervous tissues, two from testes, and two from fat bodies. These cell lines have been passaged 18-57 times, indicating they are established lines. They are maintained in EX-CELL 420 or a combination of L15 + EX-CELL 420 media. The identities of the cell lines were confirmed by DAF-PCR and their doubling times ranged from 42 to 110 h. Microscopy indicated the presence of one or more morphologically distinct cell types in each cell line. We identified a catalase gene in all seven cell lines. H₂O₂ treatment suppressed the expression of catatase and led to a reduction in catalase activity. This is the first report of cell lines established from S. endania, and these cell lines are now available to researchers worldwide on request. |
| doi_str_mv | 10.1007/s11626-018-0300-4 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_journals_2207798131</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>45179011</jstor_id><sourcerecordid>45179011</sourcerecordid><originalsourceid>FETCH-LOGICAL-c402t-e18d3abe876e8cf6d614e16d13958adc802cfe3f82589b42ab706aff104bb1403</originalsourceid><addsrcrecordid>eNp9kE9P3DAQxa2KqrvQfoBekCXUG6EztuM4x2pFAQmJA23Vm-XENmS1iRc7AW0_PUbhT0_4Mpbm997MPEK-IpwgQPU9IUomC0BVAAcoxAeyxFLwogL5dy__ocKCyRoWZD-lNeRXo_xEFhx4yVkll-TP6tZE044udv_M2IWBBk9bt9nQTTe4RG1u3DtLfQw9HW8dTWHKJQ7UxH73EGJ_TK-3wYZttjA009YMnflMPnqzSe7Lcz0gv3-e_lqdF5dXZxerH5dFK4CNhUNluWmcqqRTrZdWonAoLfK6VMa2CljrHfeKlapuBDNNvsx4jyCaBgXwA3I0-25juJtcGvU6THHIIzVjUFW1Qo6ZwplqY0gpOq-3setN3GkE_ZSknpPUOUn9lKQWWXP47Dw1vbOvipfoMsBmIOXWcOPi2-j3XL_NonUaQ_x_DcazQJRY1YDIHwE3lopN</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2207798131</pqid></control><display><type>article</type><title>Characterization of cell lines derived from the southern armyworm, Spodoptera eridania</title><source>Jstor Complete Legacy</source><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Zhang, Hongwei ; Li, Yaofa ; Reall, Tamra ; Xu, Yiyun ; Goodman, Cynthia ; Saathoff, Stephen ; Ringbauer, Joseph ; Stanley, David</creator><creatorcontrib>Zhang, Hongwei ; Li, Yaofa ; Reall, Tamra ; Xu, Yiyun ; Goodman, Cynthia ; Saathoff, Stephen ; Ringbauer, Joseph ; Stanley, David</creatorcontrib><description>Spodoptera eridania (southern armyworm) is a polyphagous pest of many plants, including field crops, vegetables, fruits, and ornamentals. Larvae are leaf feeders, defoliating many crops in the tropics and subtropics of the western hemisphere. In this study, cell lines from S. eridania were established to support research focused on the development of advanced pest management technologies. We generated seven cell lines from larval tissues: three from nervous tissues, two from testes, and two from fat bodies. These cell lines have been passaged 18-57 times, indicating they are established lines. They are maintained in EX-CELL 420 or a combination of L15 + EX-CELL 420 media. The identities of the cell lines were confirmed by DAF-PCR and their doubling times ranged from 42 to 110 h. Microscopy indicated the presence of one or more morphologically distinct cell types in each cell line. We identified a catalase gene in all seven cell lines. H₂O₂ treatment suppressed the expression of catatase and led to a reduction in catalase activity. This is the first report of cell lines established from S. endania, and these cell lines are now available to researchers worldwide on request.</description><identifier>ISSN: 1071-2690</identifier><identifier>EISSN: 1543-706X</identifier><identifier>DOI: 10.1007/s11626-018-0300-4</identifier><identifier>PMID: 30353276</identifier><language>eng</language><publisher>New York: Springer Science & Business Media LLC</publisher><subject>Animal Genetics and Genomics ; Animals ; Apoptosis ; Biomedical and Life Sciences ; Biotechnology ; Catalase ; Catalase - metabolism ; CELL AND TISSUE MODELS ; Cell Biology ; Cell Culture ; Cell cycle ; Cell Line ; Cell lines ; Cell Shape ; Crops ; Developmental Biology ; Feeders ; Gene expression ; Hydrogen peroxide ; Hydrogen Peroxide - toxicity ; Insecticides ; Insects ; Larvae ; Life Sciences ; Microscopy ; Nervous tissues ; Ornamental plants ; Penicillin ; Pest control ; Pests ; Plants (botany) ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Spodoptera - cytology ; Spodoptera eridania ; Stem Cells ; Tropical environments ; Western Hemisphere</subject><ispartof>In vitro cellular & developmental biology. Animal, 2018-12, Vol.54 (10), p.749-755</ispartof><rights>2018 Society for In Vitro Biology</rights><rights>The Society for In Vitro Biology 2018</rights><rights>Copyright Society for In Vitro Biology Dec 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-e18d3abe876e8cf6d614e16d13958adc802cfe3f82589b42ab706aff104bb1403</citedby><cites>FETCH-LOGICAL-c402t-e18d3abe876e8cf6d614e16d13958adc802cfe3f82589b42ab706aff104bb1403</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/45179011$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/45179011$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27901,27902,41464,42533,51294,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30353276$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Hongwei</creatorcontrib><creatorcontrib>Li, Yaofa</creatorcontrib><creatorcontrib>Reall, Tamra</creatorcontrib><creatorcontrib>Xu, Yiyun</creatorcontrib><creatorcontrib>Goodman, Cynthia</creatorcontrib><creatorcontrib>Saathoff, Stephen</creatorcontrib><creatorcontrib>Ringbauer, Joseph</creatorcontrib><creatorcontrib>Stanley, David</creatorcontrib><title>Characterization of cell lines derived from the southern armyworm, Spodoptera eridania</title><title>In vitro cellular & developmental biology. Animal</title><addtitle>In Vitro Cell.Dev.Biol.-Animal</addtitle><addtitle>In Vitro Cell Dev Biol Anim</addtitle><description>Spodoptera eridania (southern armyworm) is a polyphagous pest of many plants, including field crops, vegetables, fruits, and ornamentals. Larvae are leaf feeders, defoliating many crops in the tropics and subtropics of the western hemisphere. In this study, cell lines from S. eridania were established to support research focused on the development of advanced pest management technologies. We generated seven cell lines from larval tissues: three from nervous tissues, two from testes, and two from fat bodies. These cell lines have been passaged 18-57 times, indicating they are established lines. They are maintained in EX-CELL 420 or a combination of L15 + EX-CELL 420 media. The identities of the cell lines were confirmed by DAF-PCR and their doubling times ranged from 42 to 110 h. Microscopy indicated the presence of one or more morphologically distinct cell types in each cell line. We identified a catalase gene in all seven cell lines. H₂O₂ treatment suppressed the expression of catatase and led to a reduction in catalase activity. This is the first report of cell lines established from S. endania, and these cell lines are now available to researchers worldwide on request.</description><subject>Animal Genetics and Genomics</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Catalase</subject><subject>Catalase - metabolism</subject><subject>CELL AND TISSUE MODELS</subject><subject>Cell Biology</subject><subject>Cell Culture</subject><subject>Cell cycle</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Cell Shape</subject><subject>Crops</subject><subject>Developmental Biology</subject><subject>Feeders</subject><subject>Gene expression</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen Peroxide - toxicity</subject><subject>Insecticides</subject><subject>Insects</subject><subject>Larvae</subject><subject>Life Sciences</subject><subject>Microscopy</subject><subject>Nervous tissues</subject><subject>Ornamental plants</subject><subject>Penicillin</subject><subject>Pest control</subject><subject>Pests</subject><subject>Plants (botany)</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Spodoptera - cytology</subject><subject>Spodoptera eridania</subject><subject>Stem Cells</subject><subject>Tropical environments</subject><subject>Western Hemisphere</subject><issn>1071-2690</issn><issn>1543-706X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kE9P3DAQxa2KqrvQfoBekCXUG6EztuM4x2pFAQmJA23Vm-XENmS1iRc7AW0_PUbhT0_4Mpbm997MPEK-IpwgQPU9IUomC0BVAAcoxAeyxFLwogL5dy__ocKCyRoWZD-lNeRXo_xEFhx4yVkll-TP6tZE044udv_M2IWBBk9bt9nQTTe4RG1u3DtLfQw9HW8dTWHKJQ7UxH73EGJ_TK-3wYZttjA009YMnflMPnqzSe7Lcz0gv3-e_lqdF5dXZxerH5dFK4CNhUNluWmcqqRTrZdWonAoLfK6VMa2CljrHfeKlapuBDNNvsx4jyCaBgXwA3I0-25juJtcGvU6THHIIzVjUFW1Qo6ZwplqY0gpOq-3setN3GkE_ZSknpPUOUn9lKQWWXP47Dw1vbOvipfoMsBmIOXWcOPi2-j3XL_NonUaQ_x_DcazQJRY1YDIHwE3lopN</recordid><startdate>20181201</startdate><enddate>20181201</enddate><creator>Zhang, Hongwei</creator><creator>Li, Yaofa</creator><creator>Reall, Tamra</creator><creator>Xu, Yiyun</creator><creator>Goodman, Cynthia</creator><creator>Saathoff, Stephen</creator><creator>Ringbauer, Joseph</creator><creator>Stanley, David</creator><general>Springer Science & Business Media LLC</general><general>Springer US</general><general>Society for In Vitro Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>S0X</scope></search><sort><creationdate>20181201</creationdate><title>Characterization of cell lines derived from the southern armyworm, Spodoptera eridania</title><author>Zhang, Hongwei ; Li, Yaofa ; Reall, Tamra ; Xu, Yiyun ; Goodman, Cynthia ; Saathoff, Stephen ; Ringbauer, Joseph ; Stanley, David</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-e18d3abe876e8cf6d614e16d13958adc802cfe3f82589b42ab706aff104bb1403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animal Genetics and Genomics</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Catalase</topic><topic>Catalase - metabolism</topic><topic>CELL AND TISSUE MODELS</topic><topic>Cell Biology</topic><topic>Cell Culture</topic><topic>Cell cycle</topic><topic>Cell Line</topic><topic>Cell lines</topic><topic>Cell Shape</topic><topic>Crops</topic><topic>Developmental Biology</topic><topic>Feeders</topic><topic>Gene expression</topic><topic>Hydrogen peroxide</topic><topic>Hydrogen Peroxide - toxicity</topic><topic>Insecticides</topic><topic>Insects</topic><topic>Larvae</topic><topic>Life Sciences</topic><topic>Microscopy</topic><topic>Nervous tissues</topic><topic>Ornamental plants</topic><topic>Penicillin</topic><topic>Pest control</topic><topic>Pests</topic><topic>Plants (botany)</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Spodoptera - cytology</topic><topic>Spodoptera eridania</topic><topic>Stem Cells</topic><topic>Tropical environments</topic><topic>Western Hemisphere</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Hongwei</creatorcontrib><creatorcontrib>Li, Yaofa</creatorcontrib><creatorcontrib>Reall, Tamra</creatorcontrib><creatorcontrib>Xu, Yiyun</creatorcontrib><creatorcontrib>Goodman, Cynthia</creatorcontrib><creatorcontrib>Saathoff, Stephen</creatorcontrib><creatorcontrib>Ringbauer, Joseph</creatorcontrib><creatorcontrib>Stanley, David</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><jtitle>In vitro cellular & developmental biology. Animal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Hongwei</au><au>Li, Yaofa</au><au>Reall, Tamra</au><au>Xu, Yiyun</au><au>Goodman, Cynthia</au><au>Saathoff, Stephen</au><au>Ringbauer, Joseph</au><au>Stanley, David</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of cell lines derived from the southern armyworm, Spodoptera eridania</atitle><jtitle>In vitro cellular & developmental biology. Animal</jtitle><stitle>In Vitro Cell.Dev.Biol.-Animal</stitle><addtitle>In Vitro Cell Dev Biol Anim</addtitle><date>2018-12-01</date><risdate>2018</risdate><volume>54</volume><issue>10</issue><spage>749</spage><epage>755</epage><pages>749-755</pages><issn>1071-2690</issn><eissn>1543-706X</eissn><abstract>Spodoptera eridania (southern armyworm) is a polyphagous pest of many plants, including field crops, vegetables, fruits, and ornamentals. Larvae are leaf feeders, defoliating many crops in the tropics and subtropics of the western hemisphere. In this study, cell lines from S. eridania were established to support research focused on the development of advanced pest management technologies. We generated seven cell lines from larval tissues: three from nervous tissues, two from testes, and two from fat bodies. These cell lines have been passaged 18-57 times, indicating they are established lines. They are maintained in EX-CELL 420 or a combination of L15 + EX-CELL 420 media. The identities of the cell lines were confirmed by DAF-PCR and their doubling times ranged from 42 to 110 h. Microscopy indicated the presence of one or more morphologically distinct cell types in each cell line. We identified a catalase gene in all seven cell lines. H₂O₂ treatment suppressed the expression of catatase and led to a reduction in catalase activity. This is the first report of cell lines established from S. endania, and these cell lines are now available to researchers worldwide on request.</abstract><cop>New York</cop><pub>Springer Science & Business Media LLC</pub><pmid>30353276</pmid><doi>10.1007/s11626-018-0300-4</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1071-2690 |
| ispartof | In vitro cellular & developmental biology. Animal, 2018-12, Vol.54 (10), p.749-755 |
| issn | 1071-2690 1543-706X |
| language | eng |
| recordid | cdi_proquest_journals_2207798131 |
| source | Jstor Complete Legacy; MEDLINE; SpringerLink Journals |
| subjects | Animal Genetics and Genomics Animals Apoptosis Biomedical and Life Sciences Biotechnology Catalase Catalase - metabolism CELL AND TISSUE MODELS Cell Biology Cell Culture Cell cycle Cell Line Cell lines Cell Shape Crops Developmental Biology Feeders Gene expression Hydrogen peroxide Hydrogen Peroxide - toxicity Insecticides Insects Larvae Life Sciences Microscopy Nervous tissues Ornamental plants Penicillin Pest control Pests Plants (botany) RNA, Messenger - genetics RNA, Messenger - metabolism Spodoptera - cytology Spodoptera eridania Stem Cells Tropical environments Western Hemisphere |
| title | Characterization of cell lines derived from the southern armyworm, Spodoptera eridania |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-10T02%3A42%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20of%20cell%20lines%20derived%20from%20the%20southern%20armyworm,%20Spodoptera%20eridania&rft.jtitle=In%20vitro%20cellular%20&%20developmental%20biology.%20Animal&rft.au=Zhang,%20Hongwei&rft.date=2018-12-01&rft.volume=54&rft.issue=10&rft.spage=749&rft.epage=755&rft.pages=749-755&rft.issn=1071-2690&rft.eissn=1543-706X&rft_id=info:doi/10.1007/s11626-018-0300-4&rft_dat=%3Cjstor_proqu%3E45179011%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2207798131&rft_id=info:pmid/30353276&rft_jstor_id=45179011&rfr_iscdi=true |