GC/MS analysis, and antioxidant and antimicrobial activities of alkaloids extracted by polar and apolar solvents from the stems of Anabasis articulata
Anabasis articulata is a plant widely used in popular medicine to treat several pathologies in Algerian Sahara, such as diabetes. It is also associated with two other plants to cure certain types of cancer. Few research studies have been conducted on phytochemical analysis and in vivo biological act...
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| Veröffentlicht in: | Medicinal chemistry research 2019-05, Vol.28 (5), p.754-767 |
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| creator | Belyagoubi-Benhammou, Nabila Belyagoubi, Larbi Gismondi, Angelo Di Marco, Gabriele Canini, Antonella Atik Bekkara, Fawzia |
| description | Anabasis articulata
is a plant widely used in popular medicine to treat several pathologies in Algerian Sahara, such as diabetes. It is also associated with two other plants to cure certain types of cancer. Few research studies have been conducted on phytochemical analysis and in vivo biological activities of this species. However, no report is available on chemical elucidation of alkaloids and their antioxidant and antimicrobial properties. This study aims to characterise alkaloids in
A. articulata
stems and to evaluate their antioxidant and antimicrobial effects. Alkaloids extraction was carried out using polar (E1; E2.1; E2.2) and apolar solvents (E3), while chemical characterisation was performed by GC/MS. In vitro antioxidant activity was assessed by tree tests: total antioxidant capacity (TAC), DPPH scavenging assay and β-carotene–linoleate inhibition. For antimicrobial activity, eight different microorganisms were tested. The results suggest that E2.2 extract had the highest TAC value (14.742 ± 0.224 mg AAE/g DM), but E3 extract presented the highest DPPH scavenging activity (EC
50
= 1.242 ± 0.168 mg/mL) and a strong β-carotene–linoleate inhibition (EC
50
= 0.943 ± 0.027 mg/mL). In addition, E3 extract had the strongest inhibitory effects against all tested microorganisms and produced inhibition zones ranging between 9 and 20 mm, while the MICs of this extract ranged from 0.781 to >100 mg/mL and the MBCs ranged between 1.562 and >100 mg/mL. Forty-nine compounds belonging to 16 families, namely cyclopeptide alkaloids, steroid alkaloids, quinoline alkaloids, camptothecin alkaloids, quinazoline alkaloids, quinazoline alkaloids, isoquinoline alkaloids, isoquinolone alkaloids, indole alkaloids, terpene indole alkaloids, pyridine/pyrrolidine alkaloids, piperidine alkaloids, pyrrolizidine alkaloids, purine alkaloids, acridone alkaloids, benzazonine alkaloids and homolycorine-type Amaryllidaceae alkaloids were identified. Therefore,
A. articulata
can be considered as a source of antioxidant and antimicrobial agents. |
| doi_str_mv | 10.1007/s00044-019-02332-6 |
| format | Article |
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is a plant widely used in popular medicine to treat several pathologies in Algerian Sahara, such as diabetes. It is also associated with two other plants to cure certain types of cancer. Few research studies have been conducted on phytochemical analysis and in vivo biological activities of this species. However, no report is available on chemical elucidation of alkaloids and their antioxidant and antimicrobial properties. This study aims to characterise alkaloids in
A. articulata
stems and to evaluate their antioxidant and antimicrobial effects. Alkaloids extraction was carried out using polar (E1; E2.1; E2.2) and apolar solvents (E3), while chemical characterisation was performed by GC/MS. In vitro antioxidant activity was assessed by tree tests: total antioxidant capacity (TAC), DPPH scavenging assay and β-carotene–linoleate inhibition. For antimicrobial activity, eight different microorganisms were tested. The results suggest that E2.2 extract had the highest TAC value (14.742 ± 0.224 mg AAE/g DM), but E3 extract presented the highest DPPH scavenging activity (EC
50
= 1.242 ± 0.168 mg/mL) and a strong β-carotene–linoleate inhibition (EC
50
= 0.943 ± 0.027 mg/mL). In addition, E3 extract had the strongest inhibitory effects against all tested microorganisms and produced inhibition zones ranging between 9 and 20 mm, while the MICs of this extract ranged from 0.781 to >100 mg/mL and the MBCs ranged between 1.562 and >100 mg/mL. Forty-nine compounds belonging to 16 families, namely cyclopeptide alkaloids, steroid alkaloids, quinoline alkaloids, camptothecin alkaloids, quinazoline alkaloids, quinazoline alkaloids, isoquinoline alkaloids, isoquinolone alkaloids, indole alkaloids, terpene indole alkaloids, pyridine/pyrrolidine alkaloids, piperidine alkaloids, pyrrolizidine alkaloids, purine alkaloids, acridone alkaloids, benzazonine alkaloids and homolycorine-type Amaryllidaceae alkaloids were identified. Therefore,
A. articulata
can be considered as a source of antioxidant and antimicrobial agents.</description><identifier>ISSN: 1054-2523</identifier><identifier>EISSN: 1554-8120</identifier><identifier>DOI: 10.1007/s00044-019-02332-6</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Alkaloids ; Antiinfectives and antibacterials ; Antimicrobial activity ; Antimicrobial agents ; Antioxidants ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Camptothecin ; Carotene ; Cell Biology ; Diabetes mellitus ; In vivo methods and tests ; Indoles ; Medicinal plants ; Microorganisms ; Organic chemistry ; Original Research ; Pharmacology/Toxicology ; Piperidine ; Pyridines ; Pyrrolizidine alkaloids ; Quinoline ; Scavenging ; Solvents ; Stems ; Steroids ; β-Carotene</subject><ispartof>Medicinal chemistry research, 2019-05, Vol.28 (5), p.754-767</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2019</rights><rights>Copyright Springer Nature B.V. 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-6f9e03a60397681cf31678e8c2da8a3bd27d9fc3434f13e72bf403f0320b121b3</citedby><cites>FETCH-LOGICAL-c319t-6f9e03a60397681cf31678e8c2da8a3bd27d9fc3434f13e72bf403f0320b121b3</cites><orcidid>0000-0001-5624-4627</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00044-019-02332-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00044-019-02332-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Belyagoubi-Benhammou, Nabila</creatorcontrib><creatorcontrib>Belyagoubi, Larbi</creatorcontrib><creatorcontrib>Gismondi, Angelo</creatorcontrib><creatorcontrib>Di Marco, Gabriele</creatorcontrib><creatorcontrib>Canini, Antonella</creatorcontrib><creatorcontrib>Atik Bekkara, Fawzia</creatorcontrib><title>GC/MS analysis, and antioxidant and antimicrobial activities of alkaloids extracted by polar and apolar solvents from the stems of Anabasis articulata</title><title>Medicinal chemistry research</title><addtitle>Med Chem Res</addtitle><description>Anabasis articulata
is a plant widely used in popular medicine to treat several pathologies in Algerian Sahara, such as diabetes. It is also associated with two other plants to cure certain types of cancer. Few research studies have been conducted on phytochemical analysis and in vivo biological activities of this species. However, no report is available on chemical elucidation of alkaloids and their antioxidant and antimicrobial properties. This study aims to characterise alkaloids in
A. articulata
stems and to evaluate their antioxidant and antimicrobial effects. Alkaloids extraction was carried out using polar (E1; E2.1; E2.2) and apolar solvents (E3), while chemical characterisation was performed by GC/MS. In vitro antioxidant activity was assessed by tree tests: total antioxidant capacity (TAC), DPPH scavenging assay and β-carotene–linoleate inhibition. For antimicrobial activity, eight different microorganisms were tested. The results suggest that E2.2 extract had the highest TAC value (14.742 ± 0.224 mg AAE/g DM), but E3 extract presented the highest DPPH scavenging activity (EC
50
= 1.242 ± 0.168 mg/mL) and a strong β-carotene–linoleate inhibition (EC
50
= 0.943 ± 0.027 mg/mL). In addition, E3 extract had the strongest inhibitory effects against all tested microorganisms and produced inhibition zones ranging between 9 and 20 mm, while the MICs of this extract ranged from 0.781 to >100 mg/mL and the MBCs ranged between 1.562 and >100 mg/mL. Forty-nine compounds belonging to 16 families, namely cyclopeptide alkaloids, steroid alkaloids, quinoline alkaloids, camptothecin alkaloids, quinazoline alkaloids, quinazoline alkaloids, isoquinoline alkaloids, isoquinolone alkaloids, indole alkaloids, terpene indole alkaloids, pyridine/pyrrolidine alkaloids, piperidine alkaloids, pyrrolizidine alkaloids, purine alkaloids, acridone alkaloids, benzazonine alkaloids and homolycorine-type Amaryllidaceae alkaloids were identified. Therefore,
A. articulata
can be considered as a source of antioxidant and antimicrobial agents.</description><subject>Alkaloids</subject><subject>Antiinfectives and antibacterials</subject><subject>Antimicrobial activity</subject><subject>Antimicrobial agents</subject><subject>Antioxidants</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Camptothecin</subject><subject>Carotene</subject><subject>Cell Biology</subject><subject>Diabetes mellitus</subject><subject>In vivo methods and tests</subject><subject>Indoles</subject><subject>Medicinal plants</subject><subject>Microorganisms</subject><subject>Organic chemistry</subject><subject>Original Research</subject><subject>Pharmacology/Toxicology</subject><subject>Piperidine</subject><subject>Pyridines</subject><subject>Pyrrolizidine alkaloids</subject><subject>Quinoline</subject><subject>Scavenging</subject><subject>Solvents</subject><subject>Stems</subject><subject>Steroids</subject><subject>β-Carotene</subject><issn>1054-2523</issn><issn>1554-8120</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kMFOwzAQRCMEEqXwA5wscSV0bSdxcqwqKEhFHICztUlscEnjYruo_RG-F9MguHFY7Vg7M5ZekpxTuKIAYuIBIMtSoFUKjHOWFgfJiOZ5lpaUwWHUEDXLGT9OTrxfAnABWT5KPuezyf0jwR67nTf-Mqo2TjB2a9q4f98r0zhbG-wINsF8mGCUJ1YT7N6ws6b1RG2DizfVknpH1rZDN4QH6W33ofrgiXZ2RcKrIj6o1b5i2mON8XOCLphm02HA0-RIY-fV2c8eJ88310-z23TxML-bTRdpw2kV0kJXCjgWwCtRlLTRnBaiVGXDWiyR1y0TbaUbnvFMU64Eq3UGXANnUFNGaz5OLobetbPvG-WDXNqNizC8ZAwEi72liC42uCIC753Scu3MCt1OUpDf_OXAX0b-cs9fFjHEh5CP5v5Fub_qf1Jf7WGKWg</recordid><startdate>20190501</startdate><enddate>20190501</enddate><creator>Belyagoubi-Benhammou, Nabila</creator><creator>Belyagoubi, Larbi</creator><creator>Gismondi, Angelo</creator><creator>Di Marco, Gabriele</creator><creator>Canini, Antonella</creator><creator>Atik Bekkara, Fawzia</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><orcidid>https://orcid.org/0000-0001-5624-4627</orcidid></search><sort><creationdate>20190501</creationdate><title>GC/MS analysis, and antioxidant and antimicrobial activities of alkaloids extracted by polar and apolar solvents from the stems of Anabasis articulata</title><author>Belyagoubi-Benhammou, Nabila ; Belyagoubi, Larbi ; Gismondi, Angelo ; Di Marco, Gabriele ; Canini, Antonella ; Atik Bekkara, Fawzia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-6f9e03a60397681cf31678e8c2da8a3bd27d9fc3434f13e72bf403f0320b121b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Alkaloids</topic><topic>Antiinfectives and antibacterials</topic><topic>Antimicrobial activity</topic><topic>Antimicrobial agents</topic><topic>Antioxidants</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Camptothecin</topic><topic>Carotene</topic><topic>Cell Biology</topic><topic>Diabetes mellitus</topic><topic>In vivo methods and tests</topic><topic>Indoles</topic><topic>Medicinal plants</topic><topic>Microorganisms</topic><topic>Organic chemistry</topic><topic>Original Research</topic><topic>Pharmacology/Toxicology</topic><topic>Piperidine</topic><topic>Pyridines</topic><topic>Pyrrolizidine alkaloids</topic><topic>Quinoline</topic><topic>Scavenging</topic><topic>Solvents</topic><topic>Stems</topic><topic>Steroids</topic><topic>β-Carotene</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Belyagoubi-Benhammou, Nabila</creatorcontrib><creatorcontrib>Belyagoubi, Larbi</creatorcontrib><creatorcontrib>Gismondi, Angelo</creatorcontrib><creatorcontrib>Di Marco, Gabriele</creatorcontrib><creatorcontrib>Canini, Antonella</creatorcontrib><creatorcontrib>Atik Bekkara, Fawzia</creatorcontrib><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Medicinal chemistry research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Belyagoubi-Benhammou, Nabila</au><au>Belyagoubi, Larbi</au><au>Gismondi, Angelo</au><au>Di Marco, Gabriele</au><au>Canini, Antonella</au><au>Atik Bekkara, Fawzia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>GC/MS analysis, and antioxidant and antimicrobial activities of alkaloids extracted by polar and apolar solvents from the stems of Anabasis articulata</atitle><jtitle>Medicinal chemistry research</jtitle><stitle>Med Chem Res</stitle><date>2019-05-01</date><risdate>2019</risdate><volume>28</volume><issue>5</issue><spage>754</spage><epage>767</epage><pages>754-767</pages><issn>1054-2523</issn><eissn>1554-8120</eissn><abstract>Anabasis articulata
is a plant widely used in popular medicine to treat several pathologies in Algerian Sahara, such as diabetes. It is also associated with two other plants to cure certain types of cancer. Few research studies have been conducted on phytochemical analysis and in vivo biological activities of this species. However, no report is available on chemical elucidation of alkaloids and their antioxidant and antimicrobial properties. This study aims to characterise alkaloids in
A. articulata
stems and to evaluate their antioxidant and antimicrobial effects. Alkaloids extraction was carried out using polar (E1; E2.1; E2.2) and apolar solvents (E3), while chemical characterisation was performed by GC/MS. In vitro antioxidant activity was assessed by tree tests: total antioxidant capacity (TAC), DPPH scavenging assay and β-carotene–linoleate inhibition. For antimicrobial activity, eight different microorganisms were tested. The results suggest that E2.2 extract had the highest TAC value (14.742 ± 0.224 mg AAE/g DM), but E3 extract presented the highest DPPH scavenging activity (EC
50
= 1.242 ± 0.168 mg/mL) and a strong β-carotene–linoleate inhibition (EC
50
= 0.943 ± 0.027 mg/mL). In addition, E3 extract had the strongest inhibitory effects against all tested microorganisms and produced inhibition zones ranging between 9 and 20 mm, while the MICs of this extract ranged from 0.781 to >100 mg/mL and the MBCs ranged between 1.562 and >100 mg/mL. Forty-nine compounds belonging to 16 families, namely cyclopeptide alkaloids, steroid alkaloids, quinoline alkaloids, camptothecin alkaloids, quinazoline alkaloids, quinazoline alkaloids, isoquinoline alkaloids, isoquinolone alkaloids, indole alkaloids, terpene indole alkaloids, pyridine/pyrrolidine alkaloids, piperidine alkaloids, pyrrolizidine alkaloids, purine alkaloids, acridone alkaloids, benzazonine alkaloids and homolycorine-type Amaryllidaceae alkaloids were identified. Therefore,
A. articulata
can be considered as a source of antioxidant and antimicrobial agents.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s00044-019-02332-6</doi><tpages>14</tpages><orcidid>https://orcid.org/0000-0001-5624-4627</orcidid></addata></record> |
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| ispartof | Medicinal chemistry research, 2019-05, Vol.28 (5), p.754-767 |
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| subjects | Alkaloids Antiinfectives and antibacterials Antimicrobial activity Antimicrobial agents Antioxidants Biochemistry Biomedical and Life Sciences Biomedicine Camptothecin Carotene Cell Biology Diabetes mellitus In vivo methods and tests Indoles Medicinal plants Microorganisms Organic chemistry Original Research Pharmacology/Toxicology Piperidine Pyridines Pyrrolizidine alkaloids Quinoline Scavenging Solvents Stems Steroids β-Carotene |
| title | GC/MS analysis, and antioxidant and antimicrobial activities of alkaloids extracted by polar and apolar solvents from the stems of Anabasis articulata |
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