Protective Efficacy of Asparagus racemosus root extract and Isoprinosine against Ionizing Radiation - induced Clastogenicity and Toxicity in Swiss Albino Mice
Objective: We aimed to evaluate anticlastogenic and radioprotective potential of Asparagus racemosus root extract (ARE) and Isoprinosine (IPR) against electron beam radiation (EBR) induced clastogenicity and toxicity in Swiss albino mice. Methodology: In the pre-radiation study, the experimental ani...
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| creator | Poonacha, Sharmila Kameyanda Bavabeedu, Satheesh Kumar Bhandary Fernandes, Ronald Nalilu, Suchetha Kumari Srinivas Bhat, Vadisha Kolakebail, Jayarama Shetty Mangattu Jose, Jerish Peter, Alex John |
| description | Objective: We aimed to evaluate anticlastogenic and radioprotective potential of Asparagus racemosus root extract (ARE) and Isoprinosine (IPR) against electron beam radiation (EBR) induced clastogenicity and toxicity in Swiss albino mice. Methodology: In the pre-radiation study, the experimental animals were orally administered ARE - 200mg and IPR - 400mg/ kg b.wt once daily for 15 consecutive days. The animals exposed to sublethal dose (6Gy) of whole body EBR. Chromosomal aberration analysis and micronucleus assay were carried out in the bone marrow cells of the experimental animals. The various types of aberrations were scored and the micronuclei in Polychromatic Erythrocytes (PCE) and Nomochromatic Erythrocytes (NCE) were recorded. Assessment of Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), was performed using mouse GM-CSF Picokine ELISA kit. Non-specific Alpha - esterase activity was determined by simultaneous azo dye coupling method. Dose Reduction Factor (DRF) was calculated to determine the protective role of ARE and IPR against EBR. Result: Treatment of mice with ARE-200 mg/kg b.wt and IPR-400mg/kg b.wt decreased the percentage of the total aberration compared to the irradiated group; significantly reduced (P |
| doi_str_mv | 10.5530/jyp.2019.11.9 |
| format | Article |
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Methodology: In the pre-radiation study, the experimental animals were orally administered ARE - 200mg and IPR - 400mg/ kg b.wt once daily for 15 consecutive days. The animals exposed to sublethal dose (6Gy) of whole body EBR. Chromosomal aberration analysis and micronucleus assay were carried out in the bone marrow cells of the experimental animals. The various types of aberrations were scored and the micronuclei in Polychromatic Erythrocytes (PCE) and Nomochromatic Erythrocytes (NCE) were recorded. Assessment of Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), was performed using mouse GM-CSF Picokine ELISA kit. Non-specific Alpha - esterase activity was determined by simultaneous azo dye coupling method. Dose Reduction Factor (DRF) was calculated to determine the protective role of ARE and IPR against EBR. Result: Treatment of mice with ARE-200 mg/kg b.wt and IPR-400mg/kg b.wt decreased the percentage of the total aberration compared to the irradiated group; significantly reduced (P<0.05) the frequency of Mn PCE and Mn NCE when compared with irradiation alone groups. Irradiation reduced the level of GM-CSF in the splenocytes which was enhanced by the pre-treatment with ARE and IPR. There was a significant increase in the number of alpha-esterase positive cells in the pre-treatment group compared to radiation control. Increase in survival percentage was observed in the pre-treated mice when compared to radiation alone group. The DRF value of 1.11 and 1.04 was observed respectively. Conclusion: The present study suggests that the antioxidant potential of ARE and IPR could be of extreme significance in offering radioprotection and may be useful in combating various free-radical and reactive oxygen species - mediated human pathological conditions.</description><identifier>ISSN: 0975-1483</identifier><identifier>EISSN: 0975-1505</identifier><identifier>DOI: 10.5530/jyp.2019.11.9</identifier><language>eng</language><publisher>Bangalore: InPharm</publisher><subject>Albinism ; Animals ; Bone marrow ; Cancer ; Chemistry ; Chromosomes ; Deoxyribonucleic acid ; DNA ; DNA damage ; Drug dosages ; Free radicals ; Laboratories ; Pharmacists ; Trends ; Variance analysis ; Vegetables</subject><ispartof>Journal of young pharmacists, 2019-01, Vol.11 (1), p.40-45</ispartof><rights>Copyright InPharm Jan/Mar 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Poonacha, Sharmila Kameyanda</creatorcontrib><creatorcontrib>Bavabeedu, Satheesh Kumar Bhandary</creatorcontrib><creatorcontrib>Fernandes, Ronald</creatorcontrib><creatorcontrib>Nalilu, Suchetha Kumari</creatorcontrib><creatorcontrib>Srinivas Bhat, Vadisha</creatorcontrib><creatorcontrib>Kolakebail, Jayarama Shetty</creatorcontrib><creatorcontrib>Mangattu Jose, Jerish</creatorcontrib><creatorcontrib>Peter, Alex John</creatorcontrib><title>Protective Efficacy of Asparagus racemosus root extract and Isoprinosine against Ionizing Radiation - induced Clastogenicity and Toxicity in Swiss Albino Mice</title><title>Journal of young pharmacists</title><description>Objective: We aimed to evaluate anticlastogenic and radioprotective potential of Asparagus racemosus root extract (ARE) and Isoprinosine (IPR) against electron beam radiation (EBR) induced clastogenicity and toxicity in Swiss albino mice. Methodology: In the pre-radiation study, the experimental animals were orally administered ARE - 200mg and IPR - 400mg/ kg b.wt once daily for 15 consecutive days. The animals exposed to sublethal dose (6Gy) of whole body EBR. Chromosomal aberration analysis and micronucleus assay were carried out in the bone marrow cells of the experimental animals. The various types of aberrations were scored and the micronuclei in Polychromatic Erythrocytes (PCE) and Nomochromatic Erythrocytes (NCE) were recorded. Assessment of Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), was performed using mouse GM-CSF Picokine ELISA kit. Non-specific Alpha - esterase activity was determined by simultaneous azo dye coupling method. Dose Reduction Factor (DRF) was calculated to determine the protective role of ARE and IPR against EBR. Result: Treatment of mice with ARE-200 mg/kg b.wt and IPR-400mg/kg b.wt decreased the percentage of the total aberration compared to the irradiated group; significantly reduced (P<0.05) the frequency of Mn PCE and Mn NCE when compared with irradiation alone groups. Irradiation reduced the level of GM-CSF in the splenocytes which was enhanced by the pre-treatment with ARE and IPR. There was a significant increase in the number of alpha-esterase positive cells in the pre-treatment group compared to radiation control. Increase in survival percentage was observed in the pre-treated mice when compared to radiation alone group. The DRF value of 1.11 and 1.04 was observed respectively. Conclusion: The present study suggests that the antioxidant potential of ARE and IPR could be of extreme significance in offering radioprotection and may be useful in combating various free-radical and reactive oxygen species - mediated human pathological conditions.</description><subject>Albinism</subject><subject>Animals</subject><subject>Bone marrow</subject><subject>Cancer</subject><subject>Chemistry</subject><subject>Chromosomes</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA damage</subject><subject>Drug dosages</subject><subject>Free radicals</subject><subject>Laboratories</subject><subject>Pharmacists</subject><subject>Trends</subject><subject>Variance analysis</subject><subject>Vegetables</subject><issn>0975-1483</issn><issn>0975-1505</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNo1UctOwzAQjBBIVKVH7pY4J_gRN_GxqgpUKgJBOVuOY0euWjvYDjR8DN9KQmEvO7uandFqkuQawYxSAm93fZthiFiGUMbOkglkBU0RhfT8H-cluUxmIezgWAUsCJsk38_eRSWj-VBgpbWRQvbAabAIrfCi6QLwQqqDCyNyLgJ1jMMmAmFrsA6u9ca6YKwCohHGhgjWzpovYxvwImojonEWpMDYupOqBsu9CNE1yhppYv8rsnXH02AseP00IYDFvhpEwaOR6iq50GIf1OyvT5O3u9V2-ZBunu7Xy8UmlYjBmErNciqKslSEYlmxGupCEphDVWqJ5piVUOAqz3FOFBECseEKz_NKS60JLSoyTW5Ouq13750Kke9c5-1gyTGGNKcYYjKw0hNLeheCV5oP7x-E7zmCfEyBDynwMQWOEGfkB034fZQ</recordid><startdate>20190101</startdate><enddate>20190101</enddate><creator>Poonacha, Sharmila Kameyanda</creator><creator>Bavabeedu, Satheesh Kumar Bhandary</creator><creator>Fernandes, Ronald</creator><creator>Nalilu, Suchetha Kumari</creator><creator>Srinivas Bhat, Vadisha</creator><creator>Kolakebail, Jayarama Shetty</creator><creator>Mangattu Jose, Jerish</creator><creator>Peter, Alex John</creator><general>InPharm</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>KB0</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope></search><sort><creationdate>20190101</creationdate><title>Protective Efficacy of Asparagus racemosus root extract and Isoprinosine against Ionizing Radiation - induced Clastogenicity and Toxicity in Swiss Albino Mice</title><author>Poonacha, Sharmila Kameyanda ; Bavabeedu, Satheesh Kumar Bhandary ; Fernandes, Ronald ; Nalilu, Suchetha Kumari ; Srinivas Bhat, Vadisha ; Kolakebail, Jayarama Shetty ; Mangattu Jose, Jerish ; Peter, Alex John</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c190t-cf945a788e352cb9d0f7c3040e8fc162980a2b44243e3aa19190264bfcff357b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Albinism</topic><topic>Animals</topic><topic>Bone marrow</topic><topic>Cancer</topic><topic>Chemistry</topic><topic>Chromosomes</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA damage</topic><topic>Drug dosages</topic><topic>Free radicals</topic><topic>Laboratories</topic><topic>Pharmacists</topic><topic>Trends</topic><topic>Variance analysis</topic><topic>Vegetables</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Poonacha, Sharmila Kameyanda</creatorcontrib><creatorcontrib>Bavabeedu, Satheesh Kumar Bhandary</creatorcontrib><creatorcontrib>Fernandes, Ronald</creatorcontrib><creatorcontrib>Nalilu, Suchetha Kumari</creatorcontrib><creatorcontrib>Srinivas Bhat, Vadisha</creatorcontrib><creatorcontrib>Kolakebail, Jayarama Shetty</creatorcontrib><creatorcontrib>Mangattu Jose, Jerish</creatorcontrib><creatorcontrib>Peter, Alex John</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><jtitle>Journal of young pharmacists</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Poonacha, Sharmila Kameyanda</au><au>Bavabeedu, Satheesh Kumar Bhandary</au><au>Fernandes, Ronald</au><au>Nalilu, Suchetha Kumari</au><au>Srinivas Bhat, Vadisha</au><au>Kolakebail, Jayarama Shetty</au><au>Mangattu Jose, Jerish</au><au>Peter, Alex John</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective Efficacy of Asparagus racemosus root extract and Isoprinosine against Ionizing Radiation - induced Clastogenicity and Toxicity in Swiss Albino Mice</atitle><jtitle>Journal of young pharmacists</jtitle><date>2019-01-01</date><risdate>2019</risdate><volume>11</volume><issue>1</issue><spage>40</spage><epage>45</epage><pages>40-45</pages><issn>0975-1483</issn><eissn>0975-1505</eissn><abstract>Objective: We aimed to evaluate anticlastogenic and radioprotective potential of Asparagus racemosus root extract (ARE) and Isoprinosine (IPR) against electron beam radiation (EBR) induced clastogenicity and toxicity in Swiss albino mice. Methodology: In the pre-radiation study, the experimental animals were orally administered ARE - 200mg and IPR - 400mg/ kg b.wt once daily for 15 consecutive days. The animals exposed to sublethal dose (6Gy) of whole body EBR. Chromosomal aberration analysis and micronucleus assay were carried out in the bone marrow cells of the experimental animals. The various types of aberrations were scored and the micronuclei in Polychromatic Erythrocytes (PCE) and Nomochromatic Erythrocytes (NCE) were recorded. Assessment of Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), was performed using mouse GM-CSF Picokine ELISA kit. Non-specific Alpha - esterase activity was determined by simultaneous azo dye coupling method. Dose Reduction Factor (DRF) was calculated to determine the protective role of ARE and IPR against EBR. Result: Treatment of mice with ARE-200 mg/kg b.wt and IPR-400mg/kg b.wt decreased the percentage of the total aberration compared to the irradiated group; significantly reduced (P<0.05) the frequency of Mn PCE and Mn NCE when compared with irradiation alone groups. Irradiation reduced the level of GM-CSF in the splenocytes which was enhanced by the pre-treatment with ARE and IPR. There was a significant increase in the number of alpha-esterase positive cells in the pre-treatment group compared to radiation control. Increase in survival percentage was observed in the pre-treated mice when compared to radiation alone group. The DRF value of 1.11 and 1.04 was observed respectively. Conclusion: The present study suggests that the antioxidant potential of ARE and IPR could be of extreme significance in offering radioprotection and may be useful in combating various free-radical and reactive oxygen species - mediated human pathological conditions.</abstract><cop>Bangalore</cop><pub>InPharm</pub><doi>10.5530/jyp.2019.11.9</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Albinism Animals Bone marrow Cancer Chemistry Chromosomes Deoxyribonucleic acid DNA DNA damage Drug dosages Free radicals Laboratories Pharmacists Trends Variance analysis Vegetables |
| title | Protective Efficacy of Asparagus racemosus root extract and Isoprinosine against Ionizing Radiation - induced Clastogenicity and Toxicity in Swiss Albino Mice |
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