Down-regulation of an inhibitor of cell growth, transmembrane protein 34 (TMEM34), in anaplastic thyroid cancer
Ansaplastic thyroid cancer (ATC) is one of the most lethal malignancies, but the carcinogenic mechanism of ATC has not been clarified. Recently, we performed a cDNA microarray analysis and identified transmembrane protein 34 (TMEM34) that down-regulated in anaplastic thyroid cancer cell lines (ACL)s...
Gespeichert in:
| Veröffentlicht in: | Journal of cancer research and clinical oncology 2007-04, Vol.133 (4), p.213-218 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 218 |
|---|---|
| container_issue | 4 |
| container_start_page | 213 |
| container_title | Journal of cancer research and clinical oncology |
| container_volume | 133 |
| creator | AKAISHI, J ONDA, M OKAMOTO, J MIYAMOTO, S NAGAHAMA, M ITO, K YOSHIDA, A SHIMIZU, K |
| description | Ansaplastic thyroid cancer (ATC) is one of the most lethal malignancies, but the carcinogenic mechanism of ATC has not been clarified. Recently, we performed a cDNA microarray analysis and identified transmembrane protein 34 (TMEM34) that down-regulated in anaplastic thyroid cancer cell lines (ACL)s as compared to normal thyroid tissues.
To investigate the role of TMEM34 in ATC carcinogenesis, we examined expression levels of TMEM34 in ACLs as well as differentiated thyroid cancers (DTC)s and normal human tissues. To explore the effect of TMEM34 in ATC development, cell-growth assays with KTA2 cells were performed.
Expression of TMEM34 was down-regulated in all 11 ACLs, as compared to either normal thyroid tissues or cell lines derived from papillary or follicular thyroid cancers. TMEM34 was expressed ubiquitously in normal human tissues tested. Transfection of TMEM34 into KTA2 cells led to inhibition of cell growth.
Our findings suggest that TMEM34 might be a tumor suppressor gene, associated with the development of ATC from DTC. |
| doi_str_mv | 10.1007/s00432-006-0159-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_220544264</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1215238201</sourcerecordid><originalsourceid>FETCH-LOGICAL-c422t-34d85491db6ec77113c8b82c1548aa201cc462bcec85d1c2587aba69215cc0dd3</originalsourceid><addsrcrecordid>eNpFkM1qGzEURkVpqN20D9BNEYVCCplGVz8jzTIkbhNIyCZZC80dOZYZj1xJxuTtK2NDVpcrzvdJOoR8A_YbGNNXmTEpeMNY2zBQXWM-kDkcTkAI9ZHMGWhoFId2Rj7nvGZ1V5p_IjPQTPNWdnMSb-N-apJ_3Y2uhDjRuKRuomFahT6UmA47-nGkrynuy-qSluSmvPGbvk5PtykWHyYqJL14flw8CvnrsoZrhduOLpeAtKzeUgwDRTehT1_I2dKN2X89zXPy8mfxfHPXPDz9vb-5fmhQcl4aIQejZAdD33rUGkCg6Q1HUNI4xxkgypb36NGoAZAro13v2o6DQmTDIM7Jj2NvfeG_nc_FruMuTfVKyzlTUtbvVwiOEKaYc_JLu01h49KbBWYPhu3RsK2G7cGwNTXz_VS86zd-eE-clFbg5wlwGd24rJ4w5HfOKG1kx8R_OJaCig</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>220544264</pqid></control><display><type>article</type><title>Down-regulation of an inhibitor of cell growth, transmembrane protein 34 (TMEM34), in anaplastic thyroid cancer</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>AKAISHI, J ; ONDA, M ; OKAMOTO, J ; MIYAMOTO, S ; NAGAHAMA, M ; ITO, K ; YOSHIDA, A ; SHIMIZU, K</creator><creatorcontrib>AKAISHI, J ; ONDA, M ; OKAMOTO, J ; MIYAMOTO, S ; NAGAHAMA, M ; ITO, K ; YOSHIDA, A ; SHIMIZU, K</creatorcontrib><description>Ansaplastic thyroid cancer (ATC) is one of the most lethal malignancies, but the carcinogenic mechanism of ATC has not been clarified. Recently, we performed a cDNA microarray analysis and identified transmembrane protein 34 (TMEM34) that down-regulated in anaplastic thyroid cancer cell lines (ACL)s as compared to normal thyroid tissues.
To investigate the role of TMEM34 in ATC carcinogenesis, we examined expression levels of TMEM34 in ACLs as well as differentiated thyroid cancers (DTC)s and normal human tissues. To explore the effect of TMEM34 in ATC development, cell-growth assays with KTA2 cells were performed.
Expression of TMEM34 was down-regulated in all 11 ACLs, as compared to either normal thyroid tissues or cell lines derived from papillary or follicular thyroid cancers. TMEM34 was expressed ubiquitously in normal human tissues tested. Transfection of TMEM34 into KTA2 cells led to inhibition of cell growth.
Our findings suggest that TMEM34 might be a tumor suppressor gene, associated with the development of ATC from DTC.</description><identifier>ISSN: 0171-5216</identifier><identifier>EISSN: 1432-1335</identifier><identifier>DOI: 10.1007/s00432-006-0159-8</identifier><identifier>PMID: 17072649</identifier><identifier>CODEN: JCROD7</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Antineoplastic agents ; Biological and medical sciences ; Carcinoma - genetics ; Carcinoma - metabolism ; Carcinoma - pathology ; Cell growth ; Cell Growth Processes - genetics ; Cell Line, Tumor ; Cloning, Molecular ; DNA Primers ; Down-Regulation ; Endocrinopathies ; Gene expression ; Gene Expression Profiling - methods ; Gene Expression Regulation, Neoplastic ; Genes, Tumor Suppressor ; Humans ; Malignant tumors ; Medical sciences ; Membrane Proteins - chemistry ; Membrane Proteins - metabolism ; Non tumoral diseases. Target tissue resistance. Benign neoplasms ; Oligonucleotide Array Sequence Analysis - methods ; Pharmacology. Drug treatments ; Proteins ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Neoplasm - genetics ; RNA, Neoplasm - isolation & purification ; Thyroid cancer ; Thyroid diseases ; Thyroid Neoplasms - genetics ; Thyroid Neoplasms - metabolism ; Thyroid Neoplasms - pathology ; Thyroid. Thyroid axis (diseases) ; Transfection ; Tumor Suppressor Proteins - chemistry ; Tumor Suppressor Proteins - metabolism</subject><ispartof>Journal of cancer research and clinical oncology, 2007-04, Vol.133 (4), p.213-218</ispartof><rights>2007 INIST-CNRS</rights><rights>Springer-Verlag 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-34d85491db6ec77113c8b82c1548aa201cc462bcec85d1c2587aba69215cc0dd3</citedby><cites>FETCH-LOGICAL-c422t-34d85491db6ec77113c8b82c1548aa201cc462bcec85d1c2587aba69215cc0dd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18578490$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17072649$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>AKAISHI, J</creatorcontrib><creatorcontrib>ONDA, M</creatorcontrib><creatorcontrib>OKAMOTO, J</creatorcontrib><creatorcontrib>MIYAMOTO, S</creatorcontrib><creatorcontrib>NAGAHAMA, M</creatorcontrib><creatorcontrib>ITO, K</creatorcontrib><creatorcontrib>YOSHIDA, A</creatorcontrib><creatorcontrib>SHIMIZU, K</creatorcontrib><title>Down-regulation of an inhibitor of cell growth, transmembrane protein 34 (TMEM34), in anaplastic thyroid cancer</title><title>Journal of cancer research and clinical oncology</title><addtitle>J Cancer Res Clin Oncol</addtitle><description>Ansaplastic thyroid cancer (ATC) is one of the most lethal malignancies, but the carcinogenic mechanism of ATC has not been clarified. Recently, we performed a cDNA microarray analysis and identified transmembrane protein 34 (TMEM34) that down-regulated in anaplastic thyroid cancer cell lines (ACL)s as compared to normal thyroid tissues.
To investigate the role of TMEM34 in ATC carcinogenesis, we examined expression levels of TMEM34 in ACLs as well as differentiated thyroid cancers (DTC)s and normal human tissues. To explore the effect of TMEM34 in ATC development, cell-growth assays with KTA2 cells were performed.
Expression of TMEM34 was down-regulated in all 11 ACLs, as compared to either normal thyroid tissues or cell lines derived from papillary or follicular thyroid cancers. TMEM34 was expressed ubiquitously in normal human tissues tested. Transfection of TMEM34 into KTA2 cells led to inhibition of cell growth.
Our findings suggest that TMEM34 might be a tumor suppressor gene, associated with the development of ATC from DTC.</description><subject>Antineoplastic agents</subject><subject>Biological and medical sciences</subject><subject>Carcinoma - genetics</subject><subject>Carcinoma - metabolism</subject><subject>Carcinoma - pathology</subject><subject>Cell growth</subject><subject>Cell Growth Processes - genetics</subject><subject>Cell Line, Tumor</subject><subject>Cloning, Molecular</subject><subject>DNA Primers</subject><subject>Down-Regulation</subject><subject>Endocrinopathies</subject><subject>Gene expression</subject><subject>Gene Expression Profiling - methods</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Genes, Tumor Suppressor</subject><subject>Humans</subject><subject>Malignant tumors</subject><subject>Medical sciences</subject><subject>Membrane Proteins - chemistry</subject><subject>Membrane Proteins - metabolism</subject><subject>Non tumoral diseases. Target tissue resistance. Benign neoplasms</subject><subject>Oligonucleotide Array Sequence Analysis - methods</subject><subject>Pharmacology. Drug treatments</subject><subject>Proteins</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Neoplasm - genetics</subject><subject>RNA, Neoplasm - isolation & purification</subject><subject>Thyroid cancer</subject><subject>Thyroid diseases</subject><subject>Thyroid Neoplasms - genetics</subject><subject>Thyroid Neoplasms - metabolism</subject><subject>Thyroid Neoplasms - pathology</subject><subject>Thyroid. Thyroid axis (diseases)</subject><subject>Transfection</subject><subject>Tumor Suppressor Proteins - chemistry</subject><subject>Tumor Suppressor Proteins - metabolism</subject><issn>0171-5216</issn><issn>1432-1335</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpFkM1qGzEURkVpqN20D9BNEYVCCplGVz8jzTIkbhNIyCZZC80dOZYZj1xJxuTtK2NDVpcrzvdJOoR8A_YbGNNXmTEpeMNY2zBQXWM-kDkcTkAI9ZHMGWhoFId2Rj7nvGZ1V5p_IjPQTPNWdnMSb-N-apJ_3Y2uhDjRuKRuomFahT6UmA47-nGkrynuy-qSluSmvPGbvk5PtykWHyYqJL14flw8CvnrsoZrhduOLpeAtKzeUgwDRTehT1_I2dKN2X89zXPy8mfxfHPXPDz9vb-5fmhQcl4aIQejZAdD33rUGkCg6Q1HUNI4xxkgypb36NGoAZAro13v2o6DQmTDIM7Jj2NvfeG_nc_FruMuTfVKyzlTUtbvVwiOEKaYc_JLu01h49KbBWYPhu3RsK2G7cGwNTXz_VS86zd-eE-clFbg5wlwGd24rJ4w5HfOKG1kx8R_OJaCig</recordid><startdate>20070401</startdate><enddate>20070401</enddate><creator>AKAISHI, J</creator><creator>ONDA, M</creator><creator>OKAMOTO, J</creator><creator>MIYAMOTO, S</creator><creator>NAGAHAMA, M</creator><creator>ITO, K</creator><creator>YOSHIDA, A</creator><creator>SHIMIZU, K</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope></search><sort><creationdate>20070401</creationdate><title>Down-regulation of an inhibitor of cell growth, transmembrane protein 34 (TMEM34), in anaplastic thyroid cancer</title><author>AKAISHI, J ; ONDA, M ; OKAMOTO, J ; MIYAMOTO, S ; NAGAHAMA, M ; ITO, K ; YOSHIDA, A ; SHIMIZU, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-34d85491db6ec77113c8b82c1548aa201cc462bcec85d1c2587aba69215cc0dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Antineoplastic agents</topic><topic>Biological and medical sciences</topic><topic>Carcinoma - genetics</topic><topic>Carcinoma - metabolism</topic><topic>Carcinoma - pathology</topic><topic>Cell growth</topic><topic>Cell Growth Processes - genetics</topic><topic>Cell Line, Tumor</topic><topic>Cloning, Molecular</topic><topic>DNA Primers</topic><topic>Down-Regulation</topic><topic>Endocrinopathies</topic><topic>Gene expression</topic><topic>Gene Expression Profiling - methods</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Genes, Tumor Suppressor</topic><topic>Humans</topic><topic>Malignant tumors</topic><topic>Medical sciences</topic><topic>Membrane Proteins - chemistry</topic><topic>Membrane Proteins - metabolism</topic><topic>Non tumoral diseases. Target tissue resistance. Benign neoplasms</topic><topic>Oligonucleotide Array Sequence Analysis - methods</topic><topic>Pharmacology. Drug treatments</topic><topic>Proteins</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Neoplasm - genetics</topic><topic>RNA, Neoplasm - isolation & purification</topic><topic>Thyroid cancer</topic><topic>Thyroid diseases</topic><topic>Thyroid Neoplasms - genetics</topic><topic>Thyroid Neoplasms - metabolism</topic><topic>Thyroid Neoplasms - pathology</topic><topic>Thyroid. Thyroid axis (diseases)</topic><topic>Transfection</topic><topic>Tumor Suppressor Proteins - chemistry</topic><topic>Tumor Suppressor Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>AKAISHI, J</creatorcontrib><creatorcontrib>ONDA, M</creatorcontrib><creatorcontrib>OKAMOTO, J</creatorcontrib><creatorcontrib>MIYAMOTO, S</creatorcontrib><creatorcontrib>NAGAHAMA, M</creatorcontrib><creatorcontrib>ITO, K</creatorcontrib><creatorcontrib>YOSHIDA, A</creatorcontrib><creatorcontrib>SHIMIZU, K</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><jtitle>Journal of cancer research and clinical oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>AKAISHI, J</au><au>ONDA, M</au><au>OKAMOTO, J</au><au>MIYAMOTO, S</au><au>NAGAHAMA, M</au><au>ITO, K</au><au>YOSHIDA, A</au><au>SHIMIZU, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Down-regulation of an inhibitor of cell growth, transmembrane protein 34 (TMEM34), in anaplastic thyroid cancer</atitle><jtitle>Journal of cancer research and clinical oncology</jtitle><addtitle>J Cancer Res Clin Oncol</addtitle><date>2007-04-01</date><risdate>2007</risdate><volume>133</volume><issue>4</issue><spage>213</spage><epage>218</epage><pages>213-218</pages><issn>0171-5216</issn><eissn>1432-1335</eissn><coden>JCROD7</coden><abstract>Ansaplastic thyroid cancer (ATC) is one of the most lethal malignancies, but the carcinogenic mechanism of ATC has not been clarified. Recently, we performed a cDNA microarray analysis and identified transmembrane protein 34 (TMEM34) that down-regulated in anaplastic thyroid cancer cell lines (ACL)s as compared to normal thyroid tissues.
To investigate the role of TMEM34 in ATC carcinogenesis, we examined expression levels of TMEM34 in ACLs as well as differentiated thyroid cancers (DTC)s and normal human tissues. To explore the effect of TMEM34 in ATC development, cell-growth assays with KTA2 cells were performed.
Expression of TMEM34 was down-regulated in all 11 ACLs, as compared to either normal thyroid tissues or cell lines derived from papillary or follicular thyroid cancers. TMEM34 was expressed ubiquitously in normal human tissues tested. Transfection of TMEM34 into KTA2 cells led to inhibition of cell growth.
Our findings suggest that TMEM34 might be a tumor suppressor gene, associated with the development of ATC from DTC.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>17072649</pmid><doi>10.1007/s00432-006-0159-8</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0171-5216 |
| ispartof | Journal of cancer research and clinical oncology, 2007-04, Vol.133 (4), p.213-218 |
| issn | 0171-5216 1432-1335 |
| language | eng |
| recordid | cdi_proquest_journals_220544264 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Antineoplastic agents Biological and medical sciences Carcinoma - genetics Carcinoma - metabolism Carcinoma - pathology Cell growth Cell Growth Processes - genetics Cell Line, Tumor Cloning, Molecular DNA Primers Down-Regulation Endocrinopathies Gene expression Gene Expression Profiling - methods Gene Expression Regulation, Neoplastic Genes, Tumor Suppressor Humans Malignant tumors Medical sciences Membrane Proteins - chemistry Membrane Proteins - metabolism Non tumoral diseases. Target tissue resistance. Benign neoplasms Oligonucleotide Array Sequence Analysis - methods Pharmacology. Drug treatments Proteins Reverse Transcriptase Polymerase Chain Reaction RNA, Neoplasm - genetics RNA, Neoplasm - isolation & purification Thyroid cancer Thyroid diseases Thyroid Neoplasms - genetics Thyroid Neoplasms - metabolism Thyroid Neoplasms - pathology Thyroid. Thyroid axis (diseases) Transfection Tumor Suppressor Proteins - chemistry Tumor Suppressor Proteins - metabolism |
| title | Down-regulation of an inhibitor of cell growth, transmembrane protein 34 (TMEM34), in anaplastic thyroid cancer |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T03%3A52%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Down-regulation%20of%20an%20inhibitor%20of%20cell%20growth,%20transmembrane%20protein%2034%20(TMEM34),%20in%20anaplastic%20thyroid%20cancer&rft.jtitle=Journal%20of%20cancer%20research%20and%20clinical%20oncology&rft.au=AKAISHI,%20J&rft.date=2007-04-01&rft.volume=133&rft.issue=4&rft.spage=213&rft.epage=218&rft.pages=213-218&rft.issn=0171-5216&rft.eissn=1432-1335&rft.coden=JCROD7&rft_id=info:doi/10.1007/s00432-006-0159-8&rft_dat=%3Cproquest_cross%3E1215238201%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=220544264&rft_id=info:pmid/17072649&rfr_iscdi=true |