Sequence characterized amplified region markers tightly linked to the mating factors of Lentinula edodes
Detecting the mating types in shiitake, Lentinula edodes (Berk.) Pegler, is important for making progress in the breeding of this mushroom and determining the compatibility of the pair to cross. Shiitake is a tetrapolar fungus with two unlinking mating factors, A factor and B factor. We screened mol...
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| Veröffentlicht in: | Genome 2004-02, Vol.47 (1), p.156-162 |
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| creator | Tanaka, A Miyazaki, K Murakami, H Shiraishi, S |
| description | Detecting the mating types in shiitake, Lentinula edodes (Berk.) Pegler, is important for making progress in the breeding of this mushroom and determining the compatibility of the pair to cross. Shiitake is a tetrapolar fungus with two unlinking mating factors, A factor and B factor. We screened molecular markers linked to the mating factors using the randomly amplified polymorphic DNA (RAPD) method to develop the mating type identification procedure. Using 147 oligonucleotide primers, a total of 6 linkage markers for the shiitake mating factors, 4 markers for the A factor and 2 markers for the B factor, were discovered with a logarithm of the odds threshold of 3.0 for linkage. Two RAPDs that perfectly segregated with each mating factor among 72 basidiospore strains were detected. Both of these RAPDs were cloned and sequenced to convert them to the sequence characterized amplified region (SCAR) markers. Four primers, two sets of primers, were designed according to the internal sequences of two RAPDs tightly linking to the A factor or B factor. Consequently, we determined the polymerase chain reaction condition for multiplex analyses of these SCAR markers. |
| doi_str_mv | 10.1139/g03-131 |
| format | Article |
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Pegler, is important for making progress in the breeding of this mushroom and determining the compatibility of the pair to cross. Shiitake is a tetrapolar fungus with two unlinking mating factors, A factor and B factor. We screened molecular markers linked to the mating factors using the randomly amplified polymorphic DNA (RAPD) method to develop the mating type identification procedure. Using 147 oligonucleotide primers, a total of 6 linkage markers for the shiitake mating factors, 4 markers for the A factor and 2 markers for the B factor, were discovered with a logarithm of the odds threshold of 3.0 for linkage. Two RAPDs that perfectly segregated with each mating factor among 72 basidiospore strains were detected. Both of these RAPDs were cloned and sequenced to convert them to the sequence characterized amplified region (SCAR) markers. Four primers, two sets of primers, were designed according to the internal sequences of two RAPDs tightly linking to the A factor or B factor. Consequently, we determined the polymerase chain reaction condition for multiplex analyses of these SCAR markers.</description><identifier>ISSN: 0831-2796</identifier><identifier>EISSN: 1480-3321</identifier><identifier>DOI: 10.1139/g03-131</identifier><identifier>PMID: 15060612</identifier><identifier>CODEN: GENOE3</identifier><language>eng</language><publisher>Ottawa, Canada: NRC Research Press</publisher><subject>A factor ; Agriculture ; B factor ; Base Sequence ; Chromosome Mapping ; Deoxyribonucleic acid ; DNA ; DNA Primers ; Genes ; Genetic linkage ; Genetic markers ; Genetic Markers - genetics ; Genomics ; genotype ; Lentinula edodes ; linkage (genetics) ; mating factors ; Molecular Sequence Data ; Mushrooms ; nucleotide sequences ; Offspring ; Plant reproduction ; polymerase chain reaction ; Polymerase Chain Reaction - methods ; Random Amplified Polymorphic DNA Technique ; Reproduction - genetics ; Sequence Analysis, DNA ; sequence characterized amplified region ; sexual reproduction ; Shiitake Mushrooms - genetics</subject><ispartof>Genome, 2004-02, Vol.47 (1), p.156-162</ispartof><rights>Copyright National Research Council of Canada Feb 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-2bd7a76ed035ab7c6c0cfc94d5d2cee4a74d322a60a4f6332e1fad37489eaef03</citedby><cites>FETCH-LOGICAL-c425t-2bd7a76ed035ab7c6c0cfc94d5d2cee4a74d322a60a4f6332e1fad37489eaef03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://cdnsciencepub.com/doi/pdf/10.1139/g03-131$$EPDF$$P50$$Gnrcresearch$$H</linktopdf><linktohtml>$$Uhttps://cdnsciencepub.com/doi/full/10.1139/g03-131$$EHTML$$P50$$Gnrcresearch$$H</linktohtml><link.rule.ids>314,776,780,2919,27901,27902,64401,64979</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15060612$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tanaka, A</creatorcontrib><creatorcontrib>Miyazaki, K</creatorcontrib><creatorcontrib>Murakami, H</creatorcontrib><creatorcontrib>Shiraishi, S</creatorcontrib><title>Sequence characterized amplified region markers tightly linked to the mating factors of Lentinula edodes</title><title>Genome</title><addtitle>Génome</addtitle><description>Detecting the mating types in shiitake, Lentinula edodes (Berk.) Pegler, is important for making progress in the breeding of this mushroom and determining the compatibility of the pair to cross. Shiitake is a tetrapolar fungus with two unlinking mating factors, A factor and B factor. We screened molecular markers linked to the mating factors using the randomly amplified polymorphic DNA (RAPD) method to develop the mating type identification procedure. Using 147 oligonucleotide primers, a total of 6 linkage markers for the shiitake mating factors, 4 markers for the A factor and 2 markers for the B factor, were discovered with a logarithm of the odds threshold of 3.0 for linkage. Two RAPDs that perfectly segregated with each mating factor among 72 basidiospore strains were detected. Both of these RAPDs were cloned and sequenced to convert them to the sequence characterized amplified region (SCAR) markers. Four primers, two sets of primers, were designed according to the internal sequences of two RAPDs tightly linking to the A factor or B factor. Consequently, we determined the polymerase chain reaction condition for multiplex analyses of these SCAR markers.</description><subject>A factor</subject><subject>Agriculture</subject><subject>B factor</subject><subject>Base Sequence</subject><subject>Chromosome Mapping</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Primers</subject><subject>Genes</subject><subject>Genetic linkage</subject><subject>Genetic markers</subject><subject>Genetic Markers - genetics</subject><subject>Genomics</subject><subject>genotype</subject><subject>Lentinula edodes</subject><subject>linkage (genetics)</subject><subject>mating factors</subject><subject>Molecular Sequence Data</subject><subject>Mushrooms</subject><subject>nucleotide sequences</subject><subject>Offspring</subject><subject>Plant reproduction</subject><subject>polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Random Amplified Polymorphic DNA Technique</subject><subject>Reproduction - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>sequence characterized amplified region</subject><subject>sexual reproduction</subject><subject>Shiitake Mushrooms - genetics</subject><issn>0831-2796</issn><issn>1480-3321</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqF0V2L1DAUBuAgijuu4j_Q4sUKQvWcpG2ay2XxCwa8WPc6ZJKTNrudZkzai_XXm2UGBEG9Ssh5eOHkZewlwntEoT4MIGoU-IhtsOmhFoLjY7aBXmDNperO2LOcbwEQhMKn7Axb6KBDvmHjNf1YabZU2dEkYxdK4Se5yuwPU_Ch3BINIc7V3qQ7SrlawjAu0301hfmuTJdYLSOV6RLmofIlIBYUfbWluTytk6nIRUf5OXvizZTpxek8ZzefPn6_-lJvv33-enW5rW3D26XmOyeN7MiBaM1O2s6C9VY1rnXcEjVGNk5wbjowje_KnoTeOCGbXpEhD-KcXRxzDymWzfKi9yFbmiYzU1yzlihVy1vxX4g9iF7BQ-KbP-BtXNNcltCcQ9sIRFXQ2yOyKeacyOtDCuXP7jWCfqhIl4p0qajIV6e4dbcn99udOing3RHMySbKZJId_5F28Xd8QvrgfIGvj9CbqM2QQtY31xxQACiJ2AvxC6KHsRo</recordid><startdate>20040201</startdate><enddate>20040201</enddate><creator>Tanaka, A</creator><creator>Miyazaki, K</creator><creator>Murakami, H</creator><creator>Shiraishi, S</creator><general>NRC Research Press</general><general>Canadian Science Publishing NRC Research Press</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FQ</scope><scope>8FV</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M3G</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20040201</creationdate><title>Sequence characterized amplified region markers tightly linked to the mating factors of Lentinula edodes</title><author>Tanaka, A ; Miyazaki, K ; Murakami, H ; Shiraishi, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-2bd7a76ed035ab7c6c0cfc94d5d2cee4a74d322a60a4f6332e1fad37489eaef03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>A factor</topic><topic>Agriculture</topic><topic>B factor</topic><topic>Base Sequence</topic><topic>Chromosome Mapping</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA Primers</topic><topic>Genes</topic><topic>Genetic linkage</topic><topic>Genetic markers</topic><topic>Genetic Markers - genetics</topic><topic>Genomics</topic><topic>genotype</topic><topic>Lentinula edodes</topic><topic>linkage (genetics)</topic><topic>mating factors</topic><topic>Molecular Sequence Data</topic><topic>Mushrooms</topic><topic>nucleotide sequences</topic><topic>Offspring</topic><topic>Plant reproduction</topic><topic>polymerase chain reaction</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Random Amplified Polymorphic DNA Technique</topic><topic>Reproduction - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>sequence characterized amplified region</topic><topic>sexual reproduction</topic><topic>Shiitake Mushrooms - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tanaka, A</creatorcontrib><creatorcontrib>Miyazaki, K</creatorcontrib><creatorcontrib>Murakami, H</creatorcontrib><creatorcontrib>Shiraishi, S</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Canadian Business & Current Affairs Database</collection><collection>Canadian Business & Current Affairs Database (Alumni Edition)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Science Database</collection><collection>CBCA Reference & Current Events</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Genome</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tanaka, A</au><au>Miyazaki, K</au><au>Murakami, H</au><au>Shiraishi, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sequence characterized amplified region markers tightly linked to the mating factors of Lentinula edodes</atitle><jtitle>Genome</jtitle><addtitle>Génome</addtitle><date>2004-02-01</date><risdate>2004</risdate><volume>47</volume><issue>1</issue><spage>156</spage><epage>162</epage><pages>156-162</pages><issn>0831-2796</issn><eissn>1480-3321</eissn><coden>GENOE3</coden><abstract>Detecting the mating types in shiitake, Lentinula edodes (Berk.) Pegler, is important for making progress in the breeding of this mushroom and determining the compatibility of the pair to cross. Shiitake is a tetrapolar fungus with two unlinking mating factors, A factor and B factor. We screened molecular markers linked to the mating factors using the randomly amplified polymorphic DNA (RAPD) method to develop the mating type identification procedure. Using 147 oligonucleotide primers, a total of 6 linkage markers for the shiitake mating factors, 4 markers for the A factor and 2 markers for the B factor, were discovered with a logarithm of the odds threshold of 3.0 for linkage. Two RAPDs that perfectly segregated with each mating factor among 72 basidiospore strains were detected. Both of these RAPDs were cloned and sequenced to convert them to the sequence characterized amplified region (SCAR) markers. Four primers, two sets of primers, were designed according to the internal sequences of two RAPDs tightly linking to the A factor or B factor. Consequently, we determined the polymerase chain reaction condition for multiplex analyses of these SCAR markers.</abstract><cop>Ottawa, Canada</cop><pub>NRC Research Press</pub><pmid>15060612</pmid><doi>10.1139/g03-131</doi><tpages>7</tpages></addata></record> |
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| subjects | A factor Agriculture B factor Base Sequence Chromosome Mapping Deoxyribonucleic acid DNA DNA Primers Genes Genetic linkage Genetic markers Genetic Markers - genetics Genomics genotype Lentinula edodes linkage (genetics) mating factors Molecular Sequence Data Mushrooms nucleotide sequences Offspring Plant reproduction polymerase chain reaction Polymerase Chain Reaction - methods Random Amplified Polymorphic DNA Technique Reproduction - genetics Sequence Analysis, DNA sequence characterized amplified region sexual reproduction Shiitake Mushrooms - genetics |
| title | Sequence characterized amplified region markers tightly linked to the mating factors of Lentinula edodes |
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