Identification of highly transformable wheat genotypes for mass production of fertile transgenic plants
The efficiency of wheat biolistic transformation systems strongly depends on the bombardment parameters, the condition of the donor plant, and the plant genotype chosen for the transformation process. This paper analyzes the transformation efficiency of the 129 wheat sister lines generically called...
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| Veröffentlicht in: | Genome 2002-04, Vol.45 (2), p.421-430 |
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| creator | Pellegrineschi, A Noguera, L.M Skovmand, B Brito, R.M Velazquez, L Salgado, M.M Hernandez, R Warburton, M Hoisington, D |
| description | The efficiency of wheat biolistic transformation systems strongly depends on the bombardment parameters, the condition of the donor plant, and the plant genotype chosen for the transformation process. This paper analyzes the transformation efficiency of the 129 wheat sister lines generically called 'Bobwhite', originally obtained from the cross 'Aurora'//'Kalyan'/'Bluebird 3'/'Woodpecker'. A number of factors influencing the transformation were examined, such as the ability to produce embryogenic callus, regeneration in selection medium, and overall transformation performance. Of the 129 genotypes evaluated, eight demonstrated transformation efficiencies above 60% (60 independent transgenic events per 100 immature embryos bombarded). Among the eight genotypes identified, we studied agronomic characteristics such as earliness to identify the most adaptable line(s) for different lab conditions. 'Bobwhite' SH 98 26 was identified as a super-transformable wheat line. |
| doi_str_mv | 10.1139/g01-154 |
| format | Article |
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This paper analyzes the transformation efficiency of the 129 wheat sister lines generically called 'Bobwhite', originally obtained from the cross 'Aurora'//'Kalyan'/'Bluebird 3'/'Woodpecker'. A number of factors influencing the transformation were examined, such as the ability to produce embryogenic callus, regeneration in selection medium, and overall transformation performance. Of the 129 genotypes evaluated, eight demonstrated transformation efficiencies above 60% (60 independent transgenic events per 100 immature embryos bombarded). Among the eight genotypes identified, we studied agronomic characteristics such as earliness to identify the most adaptable line(s) for different lab conditions. 'Bobwhite' SH 98 26 was identified as a super-transformable wheat line.</description><identifier>ISSN: 0831-2796</identifier><identifier>EISSN: 1480-3321</identifier><identifier>DOI: 10.1139/g01-154</identifier><identifier>PMID: 11962639</identifier><identifier>CODEN: GENOE3</identifier><language>eng</language><publisher>Ottawa, Canada: NRC Research Press</publisher><subject>agronomic traits ; Biolistics ; callus ; Cells, Cultured ; efficiency ; embryo (plant) ; Fertility ; genetic transformation ; Genotype ; Genotypes ; Phenotype ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - growth & development ; Transformation, Genetic ; Transgenic plants ; Triticum - genetics ; Triticum aestivum ; Wheat</subject><ispartof>Genome, 2002-04, Vol.45 (2), p.421-430</ispartof><rights>Copyright National Research Council of Canada Apr 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c556t-2c20d3b18221ef962f6459b9dd6b42102cdc4adee078ef67848c41b47a86d2633</citedby><cites>FETCH-LOGICAL-c556t-2c20d3b18221ef962f6459b9dd6b42102cdc4adee078ef67848c41b47a86d2633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://cdnsciencepub.com/doi/pdf/10.1139/g01-154$$EPDF$$P50$$Gnrcresearch$$H</linktopdf><linktohtml>$$Uhttps://cdnsciencepub.com/doi/full/10.1139/g01-154$$EHTML$$P50$$Gnrcresearch$$H</linktohtml><link.rule.ids>314,777,781,2919,27905,27906,64407,64985</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11962639$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pellegrineschi, A</creatorcontrib><creatorcontrib>Noguera, L.M</creatorcontrib><creatorcontrib>Skovmand, B</creatorcontrib><creatorcontrib>Brito, R.M</creatorcontrib><creatorcontrib>Velazquez, L</creatorcontrib><creatorcontrib>Salgado, M.M</creatorcontrib><creatorcontrib>Hernandez, R</creatorcontrib><creatorcontrib>Warburton, M</creatorcontrib><creatorcontrib>Hoisington, D</creatorcontrib><title>Identification of highly transformable wheat genotypes for mass production of fertile transgenic plants</title><title>Genome</title><addtitle>Génome</addtitle><description>The efficiency of wheat biolistic transformation systems strongly depends on the bombardment parameters, the condition of the donor plant, and the plant genotype chosen for the transformation process. This paper analyzes the transformation efficiency of the 129 wheat sister lines generically called 'Bobwhite', originally obtained from the cross 'Aurora'//'Kalyan'/'Bluebird 3'/'Woodpecker'. A number of factors influencing the transformation were examined, such as the ability to produce embryogenic callus, regeneration in selection medium, and overall transformation performance. Of the 129 genotypes evaluated, eight demonstrated transformation efficiencies above 60% (60 independent transgenic events per 100 immature embryos bombarded). Among the eight genotypes identified, we studied agronomic characteristics such as earliness to identify the most adaptable line(s) for different lab conditions. 'Bobwhite' SH 98 26 was identified as a super-transformable wheat line.</description><subject>agronomic traits</subject><subject>Biolistics</subject><subject>callus</subject><subject>Cells, Cultured</subject><subject>efficiency</subject><subject>embryo (plant)</subject><subject>Fertility</subject><subject>genetic transformation</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>Phenotype</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - growth & development</subject><subject>Transformation, Genetic</subject><subject>Transgenic plants</subject><subject>Triticum - genetics</subject><subject>Triticum aestivum</subject><subject>Wheat</subject><issn>0831-2796</issn><issn>1480-3321</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkU2L1TAUhoMoznUU_4EGFwpCNSdJ02Qpgx8DAy501iHNR2-HtqlJitx_b3SuXlDUTbLIc948hxehx0BeATD1eiDQQMvvoB1wSRrGKNxFOyIZNLRT4gw9yPmGECBMwX10BqAEFUzt0HDp_FLGMFpTxrjgGPB-HPbTAZdklhximk0_efx1703Bg19iOaw-4_qAZ5MzXlN0m_05G3wqY8V_DFd6tHidzFLyQ3QvmCn7R8f7HF2_e_v54kNz9fH95cWbq8a2rSgNtZQ41oOkFHyokkHwVvXKOdFzCoRaZ7lx3pNO-iA6yaXl0PPOSOHqRuwcvbjNrV5fNp-Lnsds_VQlfNyylq3oBOWgKvn8n2QHgqh6_BcEyWqkoBV89ht4E7e01HU1paStpbTkJGhTzDn5oNc0ziYdNBD9vUtdu9S1y0o-OcZt_ezdiTuWdxJbkk0-e5Ps_hd1TNGrCxV8-Xfwz2-f3sLBRG2GNGZ9_YkSYIQqKrnq2Ddq6r34</recordid><startdate>20020401</startdate><enddate>20020401</enddate><creator>Pellegrineschi, A</creator><creator>Noguera, L.M</creator><creator>Skovmand, B</creator><creator>Brito, R.M</creator><creator>Velazquez, L</creator><creator>Salgado, M.M</creator><creator>Hernandez, R</creator><creator>Warburton, M</creator><creator>Hoisington, D</creator><general>NRC Research Press</general><general>Canadian Science Publishing NRC Research Press</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FQ</scope><scope>8FV</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M3G</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>20020401</creationdate><title>Identification of highly transformable wheat genotypes for mass production of fertile transgenic plants</title><author>Pellegrineschi, A ; Noguera, L.M ; Skovmand, B ; Brito, R.M ; Velazquez, L ; Salgado, M.M ; Hernandez, R ; Warburton, M ; Hoisington, D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c556t-2c20d3b18221ef962f6459b9dd6b42102cdc4adee078ef67848c41b47a86d2633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>agronomic traits</topic><topic>Biolistics</topic><topic>callus</topic><topic>Cells, Cultured</topic><topic>efficiency</topic><topic>embryo (plant)</topic><topic>Fertility</topic><topic>genetic transformation</topic><topic>Genotype</topic><topic>Genotypes</topic><topic>Phenotype</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - growth & development</topic><topic>Transformation, Genetic</topic><topic>Transgenic plants</topic><topic>Triticum - genetics</topic><topic>Triticum aestivum</topic><topic>Wheat</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pellegrineschi, A</creatorcontrib><creatorcontrib>Noguera, L.M</creatorcontrib><creatorcontrib>Skovmand, B</creatorcontrib><creatorcontrib>Brito, R.M</creatorcontrib><creatorcontrib>Velazquez, L</creatorcontrib><creatorcontrib>Salgado, M.M</creatorcontrib><creatorcontrib>Hernandez, R</creatorcontrib><creatorcontrib>Warburton, M</creatorcontrib><creatorcontrib>Hoisington, D</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Canadian Business & Current Affairs Database</collection><collection>Canadian Business & Current Affairs Database (Alumni Edition)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Science Database</collection><collection>CBCA Reference & Current Events</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genome</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pellegrineschi, A</au><au>Noguera, L.M</au><au>Skovmand, B</au><au>Brito, R.M</au><au>Velazquez, L</au><au>Salgado, M.M</au><au>Hernandez, R</au><au>Warburton, M</au><au>Hoisington, D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of highly transformable wheat genotypes for mass production of fertile transgenic plants</atitle><jtitle>Genome</jtitle><addtitle>Génome</addtitle><date>2002-04-01</date><risdate>2002</risdate><volume>45</volume><issue>2</issue><spage>421</spage><epage>430</epage><pages>421-430</pages><issn>0831-2796</issn><eissn>1480-3321</eissn><coden>GENOE3</coden><abstract>The efficiency of wheat biolistic transformation systems strongly depends on the bombardment parameters, the condition of the donor plant, and the plant genotype chosen for the transformation process. This paper analyzes the transformation efficiency of the 129 wheat sister lines generically called 'Bobwhite', originally obtained from the cross 'Aurora'//'Kalyan'/'Bluebird 3'/'Woodpecker'. A number of factors influencing the transformation were examined, such as the ability to produce embryogenic callus, regeneration in selection medium, and overall transformation performance. Of the 129 genotypes evaluated, eight demonstrated transformation efficiencies above 60% (60 independent transgenic events per 100 immature embryos bombarded). Among the eight genotypes identified, we studied agronomic characteristics such as earliness to identify the most adaptable line(s) for different lab conditions. 'Bobwhite' SH 98 26 was identified as a super-transformable wheat line.</abstract><cop>Ottawa, Canada</cop><pub>NRC Research Press</pub><pmid>11962639</pmid><doi>10.1139/g01-154</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | agronomic traits Biolistics callus Cells, Cultured efficiency embryo (plant) Fertility genetic transformation Genotype Genotypes Phenotype Plants, Genetically Modified - genetics Plants, Genetically Modified - growth & development Transformation, Genetic Transgenic plants Triticum - genetics Triticum aestivum Wheat |
| title | Identification of highly transformable wheat genotypes for mass production of fertile transgenic plants |
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