Karyotype analysis in Hyacinthella dalmatica (Hyacinthaceae) reveals vertebrate-type telomere repeats at the chromosome ends
Chromosome analysis of three different populations of Hyacinthella dalmatica (Lallem.) Trinajsti , an endemic species of the coastal region of southeastern Europe, showed a unique chromosome number, 2n = 2x = 20, and bimodal karyotype with one large and nine smaller pairs of chromosomes. Staining wi...
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| Veröffentlicht in: | Genome 2003-12, Vol.46 (6), p.1070-1076 |
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| creator | Puizina, J Weiss-Schneeweiss, H Pedorsa-Harand, A Kamenjarin, J Trinajstic, I Riha, K Schweizer, D |
| description | Chromosome analysis of three different populations of Hyacinthella dalmatica (Lallem.) Trinajsti
, an endemic species of the coastal region of southeastern Europe, showed a unique chromosome number, 2n = 2x = 20, and bimodal karyotype with one large and nine smaller pairs of chromosomes. Staining with fluorochromes CMA
3
(chromomycin A
3
) and DAPI (4,6-diamidino-2-phenylindole) revealed heterochromatic regions associated with NORs, centromeres, and several interstitial heterochromatic bands on the longest chromosome pair. Double-target FISH with two ribosomal DNA probes revealed one locus of 5S rRNA genes in the pericentromeric region of chromosome pair 3 and one locus of 18S-5.8S-26S rRNA genes on the short arm of chromosome pair 4 in all plants and populations analyzed. Southern hybridization analysis and FISH experiments demonstrated that the distal ends of H. dalmatica chromosomes contain the vertebrate telomere (5'-TTAGGG-3') repeat type rather than the Arabidopsis (5'-TTTAGGG-3') heptamer, and so suggest that this Asparagales species along with Aloe and Othocallis contains the vertebrate-type telomere repeat.Key words: Hyacinthaceae, Hyacinthella, chromosomes, ribosomal genes, telomeric repeats. |
| doi_str_mv | 10.1139/g03-078 |
| format | Article |
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, an endemic species of the coastal region of southeastern Europe, showed a unique chromosome number, 2n = 2x = 20, and bimodal karyotype with one large and nine smaller pairs of chromosomes. Staining with fluorochromes CMA
3
(chromomycin A
3
) and DAPI (4,6-diamidino-2-phenylindole) revealed heterochromatic regions associated with NORs, centromeres, and several interstitial heterochromatic bands on the longest chromosome pair. Double-target FISH with two ribosomal DNA probes revealed one locus of 5S rRNA genes in the pericentromeric region of chromosome pair 3 and one locus of 18S-5.8S-26S rRNA genes on the short arm of chromosome pair 4 in all plants and populations analyzed. Southern hybridization analysis and FISH experiments demonstrated that the distal ends of H. dalmatica chromosomes contain the vertebrate telomere (5'-TTAGGG-3') repeat type rather than the Arabidopsis (5'-TTTAGGG-3') heptamer, and so suggest that this Asparagales species along with Aloe and Othocallis contains the vertebrate-type telomere repeat.Key words: Hyacinthaceae, Hyacinthella, chromosomes, ribosomal genes, telomeric repeats.</description><identifier>ISSN: 0831-2796</identifier><identifier>EISSN: 1480-3321</identifier><identifier>DOI: 10.1139/g03-078</identifier><identifier>PMID: 14663525</identifier><identifier>CODEN: GENOE3</identifier><language>eng</language><publisher>Ottawa, Canada: NRC Research Press</publisher><subject>Animals ; Chromosome Banding ; Chromosomes ; Chromosomes, Plant - genetics ; Coastal zone ; Endemic species ; Flowers & plants ; fluorescence in situ hybridization ; genes ; Genetics ; heterochromatin ; Hyacinthella dalmatica ; Hybridization ; In Situ Hybridization, Fluorescence - methods ; Karyotypes ; Karyotyping ; Liliaceae ; Liliaceae - genetics ; nucleolus organizer region ; Probes ; repetitive sequences ; Repetitive Sequences, Nucleic Acid - genetics ; ribosomal RNA ; Southern blotting ; Telomere - genetics ; telomeres ; telomeric repeats ; Vertebrates ; Vertebrates - genetics</subject><ispartof>Genome, 2003-12, Vol.46 (6), p.1070-1076</ispartof><rights>Copyright National Research Council of Canada Dec 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c491t-3987a0481ddbb40f6bba93a8eccad2ee6f5be46382bd59636d0cef96a1c6efc53</citedby><cites>FETCH-LOGICAL-c491t-3987a0481ddbb40f6bba93a8eccad2ee6f5be46382bd59636d0cef96a1c6efc53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://cdnsciencepub.com/doi/pdf/10.1139/g03-078$$EPDF$$P50$$Gnrcresearch$$H</linktopdf><linktohtml>$$Uhttps://cdnsciencepub.com/doi/full/10.1139/g03-078$$EHTML$$P50$$Gnrcresearch$$H</linktohtml><link.rule.ids>314,780,784,2932,27924,27925,64428,65234</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14663525$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Puizina, J</creatorcontrib><creatorcontrib>Weiss-Schneeweiss, H</creatorcontrib><creatorcontrib>Pedorsa-Harand, A</creatorcontrib><creatorcontrib>Kamenjarin, J</creatorcontrib><creatorcontrib>Trinajstic, I</creatorcontrib><creatorcontrib>Riha, K</creatorcontrib><creatorcontrib>Schweizer, D</creatorcontrib><title>Karyotype analysis in Hyacinthella dalmatica (Hyacinthaceae) reveals vertebrate-type telomere repeats at the chromosome ends</title><title>Genome</title><addtitle>Génome</addtitle><description>Chromosome analysis of three different populations of Hyacinthella dalmatica (Lallem.) Trinajsti
, an endemic species of the coastal region of southeastern Europe, showed a unique chromosome number, 2n = 2x = 20, and bimodal karyotype with one large and nine smaller pairs of chromosomes. Staining with fluorochromes CMA
3
(chromomycin A
3
) and DAPI (4,6-diamidino-2-phenylindole) revealed heterochromatic regions associated with NORs, centromeres, and several interstitial heterochromatic bands on the longest chromosome pair. Double-target FISH with two ribosomal DNA probes revealed one locus of 5S rRNA genes in the pericentromeric region of chromosome pair 3 and one locus of 18S-5.8S-26S rRNA genes on the short arm of chromosome pair 4 in all plants and populations analyzed. Southern hybridization analysis and FISH experiments demonstrated that the distal ends of H. dalmatica chromosomes contain the vertebrate telomere (5'-TTAGGG-3') repeat type rather than the Arabidopsis (5'-TTTAGGG-3') heptamer, and so suggest that this Asparagales species along with Aloe and Othocallis contains the vertebrate-type telomere repeat.Key words: Hyacinthaceae, Hyacinthella, chromosomes, ribosomal genes, telomeric repeats.</description><subject>Animals</subject><subject>Chromosome Banding</subject><subject>Chromosomes</subject><subject>Chromosomes, Plant - genetics</subject><subject>Coastal zone</subject><subject>Endemic species</subject><subject>Flowers & plants</subject><subject>fluorescence in situ hybridization</subject><subject>genes</subject><subject>Genetics</subject><subject>heterochromatin</subject><subject>Hyacinthella dalmatica</subject><subject>Hybridization</subject><subject>In Situ Hybridization, Fluorescence - methods</subject><subject>Karyotypes</subject><subject>Karyotyping</subject><subject>Liliaceae</subject><subject>Liliaceae - genetics</subject><subject>nucleolus organizer region</subject><subject>Probes</subject><subject>repetitive sequences</subject><subject>Repetitive Sequences, Nucleic Acid - genetics</subject><subject>ribosomal RNA</subject><subject>Southern blotting</subject><subject>Telomere - genetics</subject><subject>telomeres</subject><subject>telomeric repeats</subject><subject>Vertebrates</subject><subject>Vertebrates - genetics</subject><issn>0831-2796</issn><issn>1480-3321</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqF0V1rFDEUBuAgFruu4j_Q4EW1wmhOMpNJLqWoLRa80F6HM5kz3SnzZZItLPjjTd0VQaheBZInbzh5GXsG4i2Asu-uhSpEbR6wFZRGFEpJeMhWwigoZG31MXsc440QIJSFR-wYSq1VJasV-_EZw25Ou4U4TjjsYh95P_HzHfp-ShsaBuQtDiOm3iN__XsfPSGd8kC3hEPktxQSNQETFb-iEg3zSIEyWAhT5Jh4DuN-E-ZxjvmM09TGJ-yoy9fp6WFds6uPH76dnReXXz5dnL2_LHxpIRXKmhpFaaBtm6YUnW4atAoNeY-tJNJd1VCplZFNW1mtdCs8dVYjeE2dr9SanexzlzB_31JMbuyjv5ttonkbXQ1lWQLY_0IwQgrIT6zZy7_gzbwN-QOjk1JUwhqoM3q1Rz7MMQbq3BL6Mf-3A-HuanO5Npdry_L5IW7bjNT-cYeeMnizB1PwgSJh8Jt_pJ3cjw_ILW2X4Ys97HB2eB366K6-5gGVAJDSCKV-AgGSuT0</recordid><startdate>20031201</startdate><enddate>20031201</enddate><creator>Puizina, J</creator><creator>Weiss-Schneeweiss, H</creator><creator>Pedorsa-Harand, A</creator><creator>Kamenjarin, J</creator><creator>Trinajstic, I</creator><creator>Riha, K</creator><creator>Schweizer, D</creator><general>NRC Research Press</general><general>Canadian Science Publishing NRC Research Press</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FQ</scope><scope>8FV</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M3G</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20031201</creationdate><title>Karyotype analysis in Hyacinthella dalmatica (Hyacinthaceae) reveals vertebrate-type telomere repeats at the chromosome ends</title><author>Puizina, J ; Weiss-Schneeweiss, H ; Pedorsa-Harand, A ; Kamenjarin, J ; Trinajstic, I ; Riha, K ; Schweizer, D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c491t-3987a0481ddbb40f6bba93a8eccad2ee6f5be46382bd59636d0cef96a1c6efc53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Chromosome Banding</topic><topic>Chromosomes</topic><topic>Chromosomes, Plant - genetics</topic><topic>Coastal zone</topic><topic>Endemic species</topic><topic>Flowers & plants</topic><topic>fluorescence in situ hybridization</topic><topic>genes</topic><topic>Genetics</topic><topic>heterochromatin</topic><topic>Hyacinthella dalmatica</topic><topic>Hybridization</topic><topic>In Situ Hybridization, Fluorescence - methods</topic><topic>Karyotypes</topic><topic>Karyotyping</topic><topic>Liliaceae</topic><topic>Liliaceae - genetics</topic><topic>nucleolus organizer region</topic><topic>Probes</topic><topic>repetitive sequences</topic><topic>Repetitive Sequences, Nucleic Acid - genetics</topic><topic>ribosomal RNA</topic><topic>Southern blotting</topic><topic>Telomere - genetics</topic><topic>telomeres</topic><topic>telomeric repeats</topic><topic>Vertebrates</topic><topic>Vertebrates - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Puizina, J</creatorcontrib><creatorcontrib>Weiss-Schneeweiss, H</creatorcontrib><creatorcontrib>Pedorsa-Harand, A</creatorcontrib><creatorcontrib>Kamenjarin, J</creatorcontrib><creatorcontrib>Trinajstic, I</creatorcontrib><creatorcontrib>Riha, K</creatorcontrib><creatorcontrib>Schweizer, D</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Canadian Business & Current Affairs Database</collection><collection>Canadian Business & Current Affairs Database (Alumni Edition)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Science Database</collection><collection>CBCA Reference & Current Events</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genome</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Puizina, J</au><au>Weiss-Schneeweiss, H</au><au>Pedorsa-Harand, A</au><au>Kamenjarin, J</au><au>Trinajstic, I</au><au>Riha, K</au><au>Schweizer, D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Karyotype analysis in Hyacinthella dalmatica (Hyacinthaceae) reveals vertebrate-type telomere repeats at the chromosome ends</atitle><jtitle>Genome</jtitle><addtitle>Génome</addtitle><date>2003-12-01</date><risdate>2003</risdate><volume>46</volume><issue>6</issue><spage>1070</spage><epage>1076</epage><pages>1070-1076</pages><issn>0831-2796</issn><eissn>1480-3321</eissn><coden>GENOE3</coden><abstract>Chromosome analysis of three different populations of Hyacinthella dalmatica (Lallem.) Trinajsti
, an endemic species of the coastal region of southeastern Europe, showed a unique chromosome number, 2n = 2x = 20, and bimodal karyotype with one large and nine smaller pairs of chromosomes. Staining with fluorochromes CMA
3
(chromomycin A
3
) and DAPI (4,6-diamidino-2-phenylindole) revealed heterochromatic regions associated with NORs, centromeres, and several interstitial heterochromatic bands on the longest chromosome pair. Double-target FISH with two ribosomal DNA probes revealed one locus of 5S rRNA genes in the pericentromeric region of chromosome pair 3 and one locus of 18S-5.8S-26S rRNA genes on the short arm of chromosome pair 4 in all plants and populations analyzed. Southern hybridization analysis and FISH experiments demonstrated that the distal ends of H. dalmatica chromosomes contain the vertebrate telomere (5'-TTAGGG-3') repeat type rather than the Arabidopsis (5'-TTTAGGG-3') heptamer, and so suggest that this Asparagales species along with Aloe and Othocallis contains the vertebrate-type telomere repeat.Key words: Hyacinthaceae, Hyacinthella, chromosomes, ribosomal genes, telomeric repeats.</abstract><cop>Ottawa, Canada</cop><pub>NRC Research Press</pub><pmid>14663525</pmid><doi>10.1139/g03-078</doi><tpages>7</tpages></addata></record> |
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| ispartof | Genome, 2003-12, Vol.46 (6), p.1070-1076 |
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| language | eng |
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| source | NRC Research Press; MEDLINE |
| subjects | Animals Chromosome Banding Chromosomes Chromosomes, Plant - genetics Coastal zone Endemic species Flowers & plants fluorescence in situ hybridization genes Genetics heterochromatin Hyacinthella dalmatica Hybridization In Situ Hybridization, Fluorescence - methods Karyotypes Karyotyping Liliaceae Liliaceae - genetics nucleolus organizer region Probes repetitive sequences Repetitive Sequences, Nucleic Acid - genetics ribosomal RNA Southern blotting Telomere - genetics telomeres telomeric repeats Vertebrates Vertebrates - genetics |
| title | Karyotype analysis in Hyacinthella dalmatica (Hyacinthaceae) reveals vertebrate-type telomere repeats at the chromosome ends |
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