PEGylation of Ginsenoside Rg3-Entrapped Bovine Serum Albumin Nanoparticles: Preparation, Characterization, and In Vitro Biological Studies
Ginsenoside Rg3 (Rg3) is one of three triterpene saponins from red ginseng. It has important structural functions and pharmacological properties. However, due to its poor solubility, low bioavailability, and short half-life in blood circulation, its clinical application was unsuccessful for the trea...
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| Veröffentlicht in: | Journal of nanomaterials 2019-01, Vol.2019 (2019), p.1-13 |
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| creator | Dong, Yanan Zhu, Chenhui Fan, Daidi Mi, Yu Ma, Pei Hui, Junfeng Zhang, Lijun Chi, Lei |
| description | Ginsenoside Rg3 (Rg3) is one of three triterpene saponins from red ginseng. It has important structural functions and pharmacological properties. However, due to its poor solubility, low bioavailability, and short half-life in blood circulation, its clinical application was unsuccessful for the treatment of a variety of cancers. In order to overcome this limitation, this study prepared mPEGylation-Rg3 bovine serum albumin nanoparticles (mPEG-Rg3-BSA NPs). The characteristics of the NPs, such as drug entrapment efficiency, drug loading efficiency, surface morphology, thermal stability, and cytotoxicity in vitro, were investigated. The results showed that the appropriate particle size of the obtained NPs was 149.5 nm, the water solubility and stability were better than free Rg3, and the drug entrapment efficiency and drug loading efficiency were 76.56% and 17.65%, respectively. Moreover, the cytotoxicity assays of the mPEG-Rg3-BSA NPs and free Rg3 revealed that the mPEG-Rg3-BSA NPs have greater anticancer effects in HepG2 cells and A549 cells. However, the cytotoxic effect of free Rg3 was higher than the mPEG-Rg3-BSA NPs in L929 cells. The results indicated that using the mPEGylation method and selecting BSA as a carrier to form the nanodrug carrier system were effective for improving the properties of Rg3. |
| doi_str_mv | 10.1155/2019/3959037 |
| format | Article |
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It has important structural functions and pharmacological properties. However, due to its poor solubility, low bioavailability, and short half-life in blood circulation, its clinical application was unsuccessful for the treatment of a variety of cancers. In order to overcome this limitation, this study prepared mPEGylation-Rg3 bovine serum albumin nanoparticles (mPEG-Rg3-BSA NPs). The characteristics of the NPs, such as drug entrapment efficiency, drug loading efficiency, surface morphology, thermal stability, and cytotoxicity in vitro, were investigated. The results showed that the appropriate particle size of the obtained NPs was 149.5 nm, the water solubility and stability were better than free Rg3, and the drug entrapment efficiency and drug loading efficiency were 76.56% and 17.65%, respectively. Moreover, the cytotoxicity assays of the mPEG-Rg3-BSA NPs and free Rg3 revealed that the mPEG-Rg3-BSA NPs have greater anticancer effects in HepG2 cells and A549 cells. However, the cytotoxic effect of free Rg3 was higher than the mPEG-Rg3-BSA NPs in L929 cells. The results indicated that using the mPEGylation method and selecting BSA as a carrier to form the nanodrug carrier system were effective for improving the properties of Rg3.</description><identifier>ISSN: 1687-4110</identifier><identifier>EISSN: 1687-4129</identifier><identifier>DOI: 10.1155/2019/3959037</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Acids ; Anticancer properties ; Apoptosis ; Bioavailability ; Biocompatibility ; Blood circulation ; Cancer ; Cell adhesion & migration ; Chemistry ; Cytotoxicity ; Drugs ; Efficiency ; Entrapment ; Infrared imaging systems ; Microscopy ; Morphology ; MPEG encoders ; Nanomaterials ; Nanoparticles ; Pharmaceuticals ; Pharmacology ; Physiology ; Polyethylene glycol ; Proteins ; Saponins ; Serum albumin ; Solubility ; Surface stability ; Thermal stability ; Toxicity ; Video compression</subject><ispartof>Journal of nanomaterials, 2019-01, Vol.2019 (2019), p.1-13</ispartof><rights>Copyright © 2019 Lijun Zhang et al.</rights><rights>Copyright © 2019 Lijun Zhang et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. http://creativecommons.org/licenses/by/4.0</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-2e32cefc72545c1decb93d025aaa85d68e1bd0227c3990796c05ecbd176eae9c3</citedby><cites>FETCH-LOGICAL-c360t-2e32cefc72545c1decb93d025aaa85d68e1bd0227c3990796c05ecbd176eae9c3</cites><orcidid>0000-0001-9360-4644 ; 0000-0001-9798-1674</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27915,27916</link.rule.ids></links><search><contributor>Reddy, Kakarla R.</contributor><contributor>Kakarla R Reddy</contributor><creatorcontrib>Dong, Yanan</creatorcontrib><creatorcontrib>Zhu, Chenhui</creatorcontrib><creatorcontrib>Fan, Daidi</creatorcontrib><creatorcontrib>Mi, Yu</creatorcontrib><creatorcontrib>Ma, Pei</creatorcontrib><creatorcontrib>Hui, Junfeng</creatorcontrib><creatorcontrib>Zhang, Lijun</creatorcontrib><creatorcontrib>Chi, Lei</creatorcontrib><title>PEGylation of Ginsenoside Rg3-Entrapped Bovine Serum Albumin Nanoparticles: Preparation, Characterization, and In Vitro Biological Studies</title><title>Journal of nanomaterials</title><description>Ginsenoside Rg3 (Rg3) is one of three triterpene saponins from red ginseng. It has important structural functions and pharmacological properties. However, due to its poor solubility, low bioavailability, and short half-life in blood circulation, its clinical application was unsuccessful for the treatment of a variety of cancers. In order to overcome this limitation, this study prepared mPEGylation-Rg3 bovine serum albumin nanoparticles (mPEG-Rg3-BSA NPs). The characteristics of the NPs, such as drug entrapment efficiency, drug loading efficiency, surface morphology, thermal stability, and cytotoxicity in vitro, were investigated. The results showed that the appropriate particle size of the obtained NPs was 149.5 nm, the water solubility and stability were better than free Rg3, and the drug entrapment efficiency and drug loading efficiency were 76.56% and 17.65%, respectively. Moreover, the cytotoxicity assays of the mPEG-Rg3-BSA NPs and free Rg3 revealed that the mPEG-Rg3-BSA NPs have greater anticancer effects in HepG2 cells and A549 cells. However, the cytotoxic effect of free Rg3 was higher than the mPEG-Rg3-BSA NPs in L929 cells. The results indicated that using the mPEGylation method and selecting BSA as a carrier to form the nanodrug carrier system were effective for improving the properties of Rg3.</description><subject>Acids</subject><subject>Anticancer properties</subject><subject>Apoptosis</subject><subject>Bioavailability</subject><subject>Biocompatibility</subject><subject>Blood circulation</subject><subject>Cancer</subject><subject>Cell adhesion & migration</subject><subject>Chemistry</subject><subject>Cytotoxicity</subject><subject>Drugs</subject><subject>Efficiency</subject><subject>Entrapment</subject><subject>Infrared imaging systems</subject><subject>Microscopy</subject><subject>Morphology</subject><subject>MPEG encoders</subject><subject>Nanomaterials</subject><subject>Nanoparticles</subject><subject>Pharmaceuticals</subject><subject>Pharmacology</subject><subject>Physiology</subject><subject>Polyethylene glycol</subject><subject>Proteins</subject><subject>Saponins</subject><subject>Serum albumin</subject><subject>Solubility</subject><subject>Surface stability</subject><subject>Thermal stability</subject><subject>Toxicity</subject><subject>Video compression</subject><issn>1687-4110</issn><issn>1687-4129</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNqF0MtKAzEUBuBBFNTqzrUEXNqxuTQzjTtbahWKirftkCZnNDJNxmRGqY_gUxudoktX58LHOfAnyQHBJ4RwPqCYiAETXGCWbyQ7JBvl6ZBQsfnbE7yd7IbwgvGQC053ks-b6WxVycY4i1yJZsYGsC4YDej2iaVT23hZ16DR2L0ZC-gOfLtEZ9WiXRqLrqR1tfSNURWEU3TjIU4_x_po8hxb1YA3H-uNtBpdWvRoGu_Q2LjKPRklK3TXtNpA2Eu2SlkF2F_XXvJwPr2fXKTz69nl5GyeKpbhJqXAqIJS5ZQPuSIa1EIwjSmXUo64zkZAFnGkuWJC4FxkCvNoNMkzkCAU6yVH3d3au9cWQlO8uNbb-LKgNObCcJZlUfU7pbwLwUNZ1N4spV8VBBffaRffaRfrtCM_7vizsVq-m__0YachGijlnyYjSviQfQFX94ql</recordid><startdate>20190101</startdate><enddate>20190101</enddate><creator>Dong, Yanan</creator><creator>Zhu, Chenhui</creator><creator>Fan, Daidi</creator><creator>Mi, Yu</creator><creator>Ma, Pei</creator><creator>Hui, Junfeng</creator><creator>Zhang, Lijun</creator><creator>Chi, Lei</creator><general>Hindawi Publishing Corporation</general><general>Hindawi</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>D1I</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>JG9</scope><scope>KB.</scope><scope>L7M</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><orcidid>https://orcid.org/0000-0001-9360-4644</orcidid><orcidid>https://orcid.org/0000-0001-9798-1674</orcidid></search><sort><creationdate>20190101</creationdate><title>PEGylation of Ginsenoside Rg3-Entrapped Bovine Serum Albumin Nanoparticles: Preparation, Characterization, and In Vitro Biological Studies</title><author>Dong, Yanan ; 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It has important structural functions and pharmacological properties. However, due to its poor solubility, low bioavailability, and short half-life in blood circulation, its clinical application was unsuccessful for the treatment of a variety of cancers. In order to overcome this limitation, this study prepared mPEGylation-Rg3 bovine serum albumin nanoparticles (mPEG-Rg3-BSA NPs). The characteristics of the NPs, such as drug entrapment efficiency, drug loading efficiency, surface morphology, thermal stability, and cytotoxicity in vitro, were investigated. The results showed that the appropriate particle size of the obtained NPs was 149.5 nm, the water solubility and stability were better than free Rg3, and the drug entrapment efficiency and drug loading efficiency were 76.56% and 17.65%, respectively. Moreover, the cytotoxicity assays of the mPEG-Rg3-BSA NPs and free Rg3 revealed that the mPEG-Rg3-BSA NPs have greater anticancer effects in HepG2 cells and A549 cells. However, the cytotoxic effect of free Rg3 was higher than the mPEG-Rg3-BSA NPs in L929 cells. The results indicated that using the mPEGylation method and selecting BSA as a carrier to form the nanodrug carrier system were effective for improving the properties of Rg3.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><doi>10.1155/2019/3959037</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0001-9360-4644</orcidid><orcidid>https://orcid.org/0000-0001-9798-1674</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acids Anticancer properties Apoptosis Bioavailability Biocompatibility Blood circulation Cancer Cell adhesion & migration Chemistry Cytotoxicity Drugs Efficiency Entrapment Infrared imaging systems Microscopy Morphology MPEG encoders Nanomaterials Nanoparticles Pharmaceuticals Pharmacology Physiology Polyethylene glycol Proteins Saponins Serum albumin Solubility Surface stability Thermal stability Toxicity Video compression |
| title | PEGylation of Ginsenoside Rg3-Entrapped Bovine Serum Albumin Nanoparticles: Preparation, Characterization, and In Vitro Biological Studies |
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