Exogenous advanced glycosylation end products induce diabetes-like vascular dysfunction in normal rats: a factor in diabetic retinopathy
Diabetic retinopathy has been shown to be directly associated with the degree and duration of hyperglycemia, and advanced glycosylation end products (AGEs) have been implicated in this pathological process. The purpose of the experiments reported here was to study the effect of AGE deposition on ret...
Gespeichert in:
| Veröffentlicht in: | Graefe's archive for clinical and experimental ophthalmology 2003, Vol.241 (1), p.56-62 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 62 |
|---|---|
| container_issue | 1 |
| container_start_page | 56 |
| container_title | Graefe's archive for clinical and experimental ophthalmology |
| container_volume | 241 |
| creator | XUN XU ZHIPING LI DAWEI LUO YUFENG HUANG JIANFENG ZHU XIAOJUE WANG HONGHUI HU PATRICK, C. P |
| description | Diabetic retinopathy has been shown to be directly associated with the degree and duration of hyperglycemia, and advanced glycosylation end products (AGEs) have been implicated in this pathological process. The purpose of the experiments reported here was to study the effect of AGE deposition on retinal vascular damage which leads to diabetic retinopathy.
Intravenous injection of exogenous AGEs was used to treat wild-type non-diabetic Sprague-Dawley rats. One of the two retinal slides from each animal was treated using immunohistochemical staining to label retinal vascular AGE deposition, the other H&E staining for counting of capillary pericytes. The results were compared with the findings in untreated wild-type and diabetic controls and in rats treated with unmodified rat serum albumin (RSA).
After 2 weeks of continuous treatment, AGEs were identified in the retinal vascular tissue of the AGE-RSA-injected group. The average number of retinal capillary pericytes per 10x100 microscope power field was 4.313+/-0.34 (mean +/- SD) in the AGE-RSA-injected group, compared with 5.798+/-0.481 in the control group ( P |
| doi_str_mv | 10.1007/s00417-002-0575-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_220156493</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>982784261</sourcerecordid><originalsourceid>FETCH-LOGICAL-c354t-960ba2e441d78da4042eed901319ea47334dd362bf7d13367be204c242ef71703</originalsourceid><addsrcrecordid>eNpFkM9O3DAQhy1UxG6BB-CCrEocU8Z_Em96qxAUJKReQOJmTWwHss3ai50g8gY8Nt5mJS4eS_P9ZjQfIWcMfjIAdZkAJFMFAC-gVGWhDsiSSZE_wJ--kSUozoqV4E8L8j2lNWRclOyILBgvZclrsSQf1-_h2fkwJor2Db1xlj73kwlp6nHogqfOW7qNwY5mSLTzuTpqO2zc4FLRd_8cfcNkxh4jtVNqR2_-xzpPfYgb7GnEIf2iSFs0Q4i7xhzvDI359WGLw8t0Qg5b7JM73ddj8nhz_XB1W9z__XN39fu-MKKUQ1FX0CB3UjKrVhYlSO6crYEJVjuUSghprah40yrLhKhU4zhIwzPWKqZAHJMf89x80-vo0qDXYYw-r9ScAysrWYsMsRkyMaQUXau3sdtgnDQDvVOvZ_U6q9c79VrlzPl-8NhsnP1K7F1n4GIPZF_YtzHb7tIXJytQ5UqJT1jeji8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>220156493</pqid></control><display><type>article</type><title>Exogenous advanced glycosylation end products induce diabetes-like vascular dysfunction in normal rats: a factor in diabetic retinopathy</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>XUN XU ; ZHIPING LI ; DAWEI LUO ; YUFENG HUANG ; JIANFENG ZHU ; XIAOJUE WANG ; HONGHUI HU ; PATRICK, C. P</creator><creatorcontrib>XUN XU ; ZHIPING LI ; DAWEI LUO ; YUFENG HUANG ; JIANFENG ZHU ; XIAOJUE WANG ; HONGHUI HU ; PATRICK, C. P</creatorcontrib><description>Diabetic retinopathy has been shown to be directly associated with the degree and duration of hyperglycemia, and advanced glycosylation end products (AGEs) have been implicated in this pathological process. The purpose of the experiments reported here was to study the effect of AGE deposition on retinal vascular damage which leads to diabetic retinopathy.
Intravenous injection of exogenous AGEs was used to treat wild-type non-diabetic Sprague-Dawley rats. One of the two retinal slides from each animal was treated using immunohistochemical staining to label retinal vascular AGE deposition, the other H&E staining for counting of capillary pericytes. The results were compared with the findings in untreated wild-type and diabetic controls and in rats treated with unmodified rat serum albumin (RSA).
After 2 weeks of continuous treatment, AGEs were identified in the retinal vascular tissue of the AGE-RSA-injected group. The average number of retinal capillary pericytes per 10x100 microscope power field was 4.313+/-0.34 (mean +/- SD) in the AGE-RSA-injected group, compared with 5.798+/-0.481 in the control group ( P<0.01).
These experiments demonstrate that AGEs, independent of other metabolic factors, can induce vascular change resembling that of diabetic retinopathy.</description><identifier>ISSN: 0721-832X</identifier><identifier>EISSN: 1435-702X</identifier><identifier>DOI: 10.1007/s00417-002-0575-7</identifier><identifier>PMID: 12545293</identifier><identifier>CODEN: GACODL</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Animals ; Associated diseases and complications ; Biological and medical sciences ; Cell Count ; Diabetes. Impaired glucose tolerance ; Diabetic retinopathy ; Diabetic Retinopathy - chemically induced ; Diabetic Retinopathy - pathology ; Endocrine pancreas. Apud cells (diseases) ; Endocrinopathies ; Fluorescein Angiography ; Glycation End Products, Advanced - pharmacology ; Glycosylation ; Injections, Intravenous ; Medical sciences ; Ophthalmology ; Pericytes - pathology ; Rats ; Rats, Sprague-Dawley ; Retinal Vessels - drug effects ; Retinal Vessels - ultrastructure</subject><ispartof>Graefe's archive for clinical and experimental ophthalmology, 2003, Vol.241 (1), p.56-62</ispartof><rights>2003 INIST-CNRS</rights><rights>Springer-Verlag 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c354t-960ba2e441d78da4042eed901319ea47334dd362bf7d13367be204c242ef71703</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14607587$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12545293$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>XUN XU</creatorcontrib><creatorcontrib>ZHIPING LI</creatorcontrib><creatorcontrib>DAWEI LUO</creatorcontrib><creatorcontrib>YUFENG HUANG</creatorcontrib><creatorcontrib>JIANFENG ZHU</creatorcontrib><creatorcontrib>XIAOJUE WANG</creatorcontrib><creatorcontrib>HONGHUI HU</creatorcontrib><creatorcontrib>PATRICK, C. P</creatorcontrib><title>Exogenous advanced glycosylation end products induce diabetes-like vascular dysfunction in normal rats: a factor in diabetic retinopathy</title><title>Graefe's archive for clinical and experimental ophthalmology</title><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><description>Diabetic retinopathy has been shown to be directly associated with the degree and duration of hyperglycemia, and advanced glycosylation end products (AGEs) have been implicated in this pathological process. The purpose of the experiments reported here was to study the effect of AGE deposition on retinal vascular damage which leads to diabetic retinopathy.
Intravenous injection of exogenous AGEs was used to treat wild-type non-diabetic Sprague-Dawley rats. One of the two retinal slides from each animal was treated using immunohistochemical staining to label retinal vascular AGE deposition, the other H&E staining for counting of capillary pericytes. The results were compared with the findings in untreated wild-type and diabetic controls and in rats treated with unmodified rat serum albumin (RSA).
After 2 weeks of continuous treatment, AGEs were identified in the retinal vascular tissue of the AGE-RSA-injected group. The average number of retinal capillary pericytes per 10x100 microscope power field was 4.313+/-0.34 (mean +/- SD) in the AGE-RSA-injected group, compared with 5.798+/-0.481 in the control group ( P<0.01).
These experiments demonstrate that AGEs, independent of other metabolic factors, can induce vascular change resembling that of diabetic retinopathy.</description><subject>Animals</subject><subject>Associated diseases and complications</subject><subject>Biological and medical sciences</subject><subject>Cell Count</subject><subject>Diabetes. Impaired glucose tolerance</subject><subject>Diabetic retinopathy</subject><subject>Diabetic Retinopathy - chemically induced</subject><subject>Diabetic Retinopathy - pathology</subject><subject>Endocrine pancreas. Apud cells (diseases)</subject><subject>Endocrinopathies</subject><subject>Fluorescein Angiography</subject><subject>Glycation End Products, Advanced - pharmacology</subject><subject>Glycosylation</subject><subject>Injections, Intravenous</subject><subject>Medical sciences</subject><subject>Ophthalmology</subject><subject>Pericytes - pathology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Retinal Vessels - drug effects</subject><subject>Retinal Vessels - ultrastructure</subject><issn>0721-832X</issn><issn>1435-702X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpFkM9O3DAQhy1UxG6BB-CCrEocU8Z_Em96qxAUJKReQOJmTWwHss3ai50g8gY8Nt5mJS4eS_P9ZjQfIWcMfjIAdZkAJFMFAC-gVGWhDsiSSZE_wJ--kSUozoqV4E8L8j2lNWRclOyILBgvZclrsSQf1-_h2fkwJor2Db1xlj73kwlp6nHogqfOW7qNwY5mSLTzuTpqO2zc4FLRd_8cfcNkxh4jtVNqR2_-xzpPfYgb7GnEIf2iSFs0Q4i7xhzvDI359WGLw8t0Qg5b7JM73ddj8nhz_XB1W9z__XN39fu-MKKUQ1FX0CB3UjKrVhYlSO6crYEJVjuUSghprah40yrLhKhU4zhIwzPWKqZAHJMf89x80-vo0qDXYYw-r9ScAysrWYsMsRkyMaQUXau3sdtgnDQDvVOvZ_U6q9c79VrlzPl-8NhsnP1K7F1n4GIPZF_YtzHb7tIXJytQ5UqJT1jeji8</recordid><startdate>2003</startdate><enddate>2003</enddate><creator>XUN XU</creator><creator>ZHIPING LI</creator><creator>DAWEI LUO</creator><creator>YUFENG HUANG</creator><creator>JIANFENG ZHU</creator><creator>XIAOJUE WANG</creator><creator>HONGHUI HU</creator><creator>PATRICK, C. P</creator><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>2003</creationdate><title>Exogenous advanced glycosylation end products induce diabetes-like vascular dysfunction in normal rats: a factor in diabetic retinopathy</title><author>XUN XU ; ZHIPING LI ; DAWEI LUO ; YUFENG HUANG ; JIANFENG ZHU ; XIAOJUE WANG ; HONGHUI HU ; PATRICK, C. P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c354t-960ba2e441d78da4042eed901319ea47334dd362bf7d13367be204c242ef71703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Associated diseases and complications</topic><topic>Biological and medical sciences</topic><topic>Cell Count</topic><topic>Diabetes. Impaired glucose tolerance</topic><topic>Diabetic retinopathy</topic><topic>Diabetic Retinopathy - chemically induced</topic><topic>Diabetic Retinopathy - pathology</topic><topic>Endocrine pancreas. Apud cells (diseases)</topic><topic>Endocrinopathies</topic><topic>Fluorescein Angiography</topic><topic>Glycation End Products, Advanced - pharmacology</topic><topic>Glycosylation</topic><topic>Injections, Intravenous</topic><topic>Medical sciences</topic><topic>Ophthalmology</topic><topic>Pericytes - pathology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Retinal Vessels - drug effects</topic><topic>Retinal Vessels - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>XUN XU</creatorcontrib><creatorcontrib>ZHIPING LI</creatorcontrib><creatorcontrib>DAWEI LUO</creatorcontrib><creatorcontrib>YUFENG HUANG</creatorcontrib><creatorcontrib>JIANFENG ZHU</creatorcontrib><creatorcontrib>XIAOJUE WANG</creatorcontrib><creatorcontrib>HONGHUI HU</creatorcontrib><creatorcontrib>PATRICK, C. P</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Graefe's archive for clinical and experimental ophthalmology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>XUN XU</au><au>ZHIPING LI</au><au>DAWEI LUO</au><au>YUFENG HUANG</au><au>JIANFENG ZHU</au><au>XIAOJUE WANG</au><au>HONGHUI HU</au><au>PATRICK, C. P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exogenous advanced glycosylation end products induce diabetes-like vascular dysfunction in normal rats: a factor in diabetic retinopathy</atitle><jtitle>Graefe's archive for clinical and experimental ophthalmology</jtitle><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><date>2003</date><risdate>2003</risdate><volume>241</volume><issue>1</issue><spage>56</spage><epage>62</epage><pages>56-62</pages><issn>0721-832X</issn><eissn>1435-702X</eissn><coden>GACODL</coden><abstract>Diabetic retinopathy has been shown to be directly associated with the degree and duration of hyperglycemia, and advanced glycosylation end products (AGEs) have been implicated in this pathological process. The purpose of the experiments reported here was to study the effect of AGE deposition on retinal vascular damage which leads to diabetic retinopathy.
Intravenous injection of exogenous AGEs was used to treat wild-type non-diabetic Sprague-Dawley rats. One of the two retinal slides from each animal was treated using immunohistochemical staining to label retinal vascular AGE deposition, the other H&E staining for counting of capillary pericytes. The results were compared with the findings in untreated wild-type and diabetic controls and in rats treated with unmodified rat serum albumin (RSA).
After 2 weeks of continuous treatment, AGEs were identified in the retinal vascular tissue of the AGE-RSA-injected group. The average number of retinal capillary pericytes per 10x100 microscope power field was 4.313+/-0.34 (mean +/- SD) in the AGE-RSA-injected group, compared with 5.798+/-0.481 in the control group ( P<0.01).
These experiments demonstrate that AGEs, independent of other metabolic factors, can induce vascular change resembling that of diabetic retinopathy.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>12545293</pmid><doi>10.1007/s00417-002-0575-7</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0721-832X |
| ispartof | Graefe's archive for clinical and experimental ophthalmology, 2003, Vol.241 (1), p.56-62 |
| issn | 0721-832X 1435-702X |
| language | eng |
| recordid | cdi_proquest_journals_220156493 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Animals Associated diseases and complications Biological and medical sciences Cell Count Diabetes. Impaired glucose tolerance Diabetic retinopathy Diabetic Retinopathy - chemically induced Diabetic Retinopathy - pathology Endocrine pancreas. Apud cells (diseases) Endocrinopathies Fluorescein Angiography Glycation End Products, Advanced - pharmacology Glycosylation Injections, Intravenous Medical sciences Ophthalmology Pericytes - pathology Rats Rats, Sprague-Dawley Retinal Vessels - drug effects Retinal Vessels - ultrastructure |
| title | Exogenous advanced glycosylation end products induce diabetes-like vascular dysfunction in normal rats: a factor in diabetic retinopathy |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-19T14%3A47%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Exogenous%20advanced%20glycosylation%20end%20products%20induce%20diabetes-like%20vascular%20dysfunction%20in%20normal%20rats:%20a%20factor%20in%20diabetic%20retinopathy&rft.jtitle=Graefe's%20archive%20for%20clinical%20and%20experimental%20ophthalmology&rft.au=XUN%20XU&rft.date=2003&rft.volume=241&rft.issue=1&rft.spage=56&rft.epage=62&rft.pages=56-62&rft.issn=0721-832X&rft.eissn=1435-702X&rft.coden=GACODL&rft_id=info:doi/10.1007/s00417-002-0575-7&rft_dat=%3Cproquest_cross%3E982784261%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=220156493&rft_id=info:pmid/12545293&rfr_iscdi=true |