Perivascular Endothelial Implants Inhibit Intimal Hyperplasia in a Model of Arteriovenous Fistulae: A Safety and Efficacy Study in the Pig
Vascular access complications are a major problem in hemodialysis patients. Native arteriovenous fistulae, historically the preferred mode of access, have a patency rate of only 60% at 1 year. The most common mode of failure is due to progressive stenosis at the anastomotic site. We have previously...
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| Veröffentlicht in: | Journal of vascular research 2002-11, Vol.39 (6), p.524-533 |
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| creator | Nugent, Helen M. Groothuis, Adam Seifert, Philip Guerraro, J. Luis Nedelman, Mark Mohanakumar, T. Edelman, Elazer R. |
| description | Vascular access complications are a major problem in hemodialysis patients. Native arteriovenous fistulae, historically the preferred mode of access, have a patency rate of only 60% at 1 year. The most common mode of failure is due to progressive stenosis at the anastomotic site. We have previously demonstrated that perivascular endothelial cell implants inhibit intimal thickening following acute balloon injury in pigs and now seek to determine if these implants provide a similar benefit in the chronic and more complex injury model of arteriovenous anastomoses. Side-to-side femoral artery-femoral vein anastomoses were created in 24 domestic swine and the toxicological, biological and immunological responses to allogeneic endothelial cell implants were investigated 3 days and 1 and 2 months postoperatively. The anastomoses were wrapped with polymer matrices containing confluent porcine aortic endothelial cells (PAE; n = 14) or control matrices without cells (n = 10). PAE implants significantly reduced intimal hyperplasia at the anastomotic sites compared to controls by 68% (p |
| doi_str_mv | 10.1159/000067207 |
| format | Article |
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Side-to-side femoral artery-femoral vein anastomoses were created in 24 domestic swine and the toxicological, biological and immunological responses to allogeneic endothelial cell implants were investigated 3 days and 1 and 2 months postoperatively. The anastomoses were wrapped with polymer matrices containing confluent porcine aortic endothelial cells (PAE; n = 14) or control matrices without cells (n = 10). PAE implants significantly reduced intimal hyperplasia at the anastomotic sites compared to controls by 68% (p <0.05) at 2 months. The beneficial effects of the PAE implants were not due to differences in the rates of reendothelialization between the groups. No significant immunological response to the allogeneic endothelial cells that impacted on efficacy was detected in any of the pigs. No apparent toxicity was observed in any of the animals treated with endothelial implants. These data suggest that perivascular endothelial cell implants are safe and reduce early intimal hyperplasia in a porcine model of arteriovenous anastomoses.</description><identifier>ISSN: 1018-1172</identifier><identifier>EISSN: 1423-0135</identifier><identifier>DOI: 10.1159/000067207</identifier><identifier>PMID: 12566978</identifier><identifier>CODEN: JVREE9</identifier><language>eng</language><publisher>Basel, Switzerland: Karger</publisher><subject>Animals ; Antibodies - blood ; Aorta - cytology ; Arteriovenous Shunt, Surgical - methods ; Biological and medical sciences ; Endothelium, Vascular - immunology ; Endothelium, Vascular - pathology ; Endothelium, Vascular - transplantation ; Femoral Artery - immunology ; Femoral Artery - pathology ; Femoral Artery - surgery ; Gelatin Sponge, Absorbable - pharmacology ; Graft Survival - immunology ; Hemostatics - pharmacology ; Hyperplasia ; Medical sciences ; Research Paper ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; T-Lymphocytes - immunology ; Tunica Intima - immunology ; Tunica Intima - pathology ; Vascular surgery: aorta, extremities, vena cava. Surgery of the lymphatic vessels ; Vasculitis - pathology</subject><ispartof>Journal of vascular research, 2002-11, Vol.39 (6), p.524-533</ispartof><rights>2002 S. Karger AG, Basel</rights><rights>2003 INIST-CNRS</rights><rights>Copyright 2002 S. Karger AG, Basel</rights><rights>Copyright (c) 2002 S. Karger AG, Basel</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-a43695a1c67f0ddba59373d2bfdd460cc548e7efdc4940c511370777a81895e03</citedby><cites>FETCH-LOGICAL-c482t-a43695a1c67f0ddba59373d2bfdd460cc548e7efdc4940c511370777a81895e03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,2423,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14480826$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12566978$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nugent, Helen M.</creatorcontrib><creatorcontrib>Groothuis, Adam</creatorcontrib><creatorcontrib>Seifert, Philip</creatorcontrib><creatorcontrib>Guerraro, J. Luis</creatorcontrib><creatorcontrib>Nedelman, Mark</creatorcontrib><creatorcontrib>Mohanakumar, T.</creatorcontrib><creatorcontrib>Edelman, Elazer R.</creatorcontrib><title>Perivascular Endothelial Implants Inhibit Intimal Hyperplasia in a Model of Arteriovenous Fistulae: A Safety and Efficacy Study in the Pig</title><title>Journal of vascular research</title><addtitle>J Vasc Res</addtitle><description>Vascular access complications are a major problem in hemodialysis patients. Native arteriovenous fistulae, historically the preferred mode of access, have a patency rate of only 60% at 1 year. The most common mode of failure is due to progressive stenosis at the anastomotic site. We have previously demonstrated that perivascular endothelial cell implants inhibit intimal thickening following acute balloon injury in pigs and now seek to determine if these implants provide a similar benefit in the chronic and more complex injury model of arteriovenous anastomoses. Side-to-side femoral artery-femoral vein anastomoses were created in 24 domestic swine and the toxicological, biological and immunological responses to allogeneic endothelial cell implants were investigated 3 days and 1 and 2 months postoperatively. The anastomoses were wrapped with polymer matrices containing confluent porcine aortic endothelial cells (PAE; n = 14) or control matrices without cells (n = 10). PAE implants significantly reduced intimal hyperplasia at the anastomotic sites compared to controls by 68% (p <0.05) at 2 months. The beneficial effects of the PAE implants were not due to differences in the rates of reendothelialization between the groups. No significant immunological response to the allogeneic endothelial cells that impacted on efficacy was detected in any of the pigs. No apparent toxicity was observed in any of the animals treated with endothelial implants. These data suggest that perivascular endothelial cell implants are safe and reduce early intimal hyperplasia in a porcine model of arteriovenous anastomoses.</description><subject>Animals</subject><subject>Antibodies - blood</subject><subject>Aorta - cytology</subject><subject>Arteriovenous Shunt, Surgical - methods</subject><subject>Biological and medical sciences</subject><subject>Endothelium, Vascular - immunology</subject><subject>Endothelium, Vascular - pathology</subject><subject>Endothelium, Vascular - transplantation</subject><subject>Femoral Artery - immunology</subject><subject>Femoral Artery - pathology</subject><subject>Femoral Artery - surgery</subject><subject>Gelatin Sponge, Absorbable - pharmacology</subject><subject>Graft Survival - immunology</subject><subject>Hemostatics - pharmacology</subject><subject>Hyperplasia</subject><subject>Medical sciences</subject><subject>Research Paper</subject><subject>Surgery (general aspects). 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Luis</au><au>Nedelman, Mark</au><au>Mohanakumar, T.</au><au>Edelman, Elazer R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Perivascular Endothelial Implants Inhibit Intimal Hyperplasia in a Model of Arteriovenous Fistulae: A Safety and Efficacy Study in the Pig</atitle><jtitle>Journal of vascular research</jtitle><addtitle>J Vasc Res</addtitle><date>2002-11-01</date><risdate>2002</risdate><volume>39</volume><issue>6</issue><spage>524</spage><epage>533</epage><pages>524-533</pages><issn>1018-1172</issn><eissn>1423-0135</eissn><coden>JVREE9</coden><abstract>Vascular access complications are a major problem in hemodialysis patients. Native arteriovenous fistulae, historically the preferred mode of access, have a patency rate of only 60% at 1 year. The most common mode of failure is due to progressive stenosis at the anastomotic site. We have previously demonstrated that perivascular endothelial cell implants inhibit intimal thickening following acute balloon injury in pigs and now seek to determine if these implants provide a similar benefit in the chronic and more complex injury model of arteriovenous anastomoses. Side-to-side femoral artery-femoral vein anastomoses were created in 24 domestic swine and the toxicological, biological and immunological responses to allogeneic endothelial cell implants were investigated 3 days and 1 and 2 months postoperatively. The anastomoses were wrapped with polymer matrices containing confluent porcine aortic endothelial cells (PAE; n = 14) or control matrices without cells (n = 10). PAE implants significantly reduced intimal hyperplasia at the anastomotic sites compared to controls by 68% (p <0.05) at 2 months. The beneficial effects of the PAE implants were not due to differences in the rates of reendothelialization between the groups. No significant immunological response to the allogeneic endothelial cells that impacted on efficacy was detected in any of the pigs. No apparent toxicity was observed in any of the animals treated with endothelial implants. These data suggest that perivascular endothelial cell implants are safe and reduce early intimal hyperplasia in a porcine model of arteriovenous anastomoses.</abstract><cop>Basel, Switzerland</cop><pub>Karger</pub><pmid>12566978</pmid><doi>10.1159/000067207</doi><tpages>10</tpages></addata></record> |
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| ispartof | Journal of vascular research, 2002-11, Vol.39 (6), p.524-533 |
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| subjects | Animals Antibodies - blood Aorta - cytology Arteriovenous Shunt, Surgical - methods Biological and medical sciences Endothelium, Vascular - immunology Endothelium, Vascular - pathology Endothelium, Vascular - transplantation Femoral Artery - immunology Femoral Artery - pathology Femoral Artery - surgery Gelatin Sponge, Absorbable - pharmacology Graft Survival - immunology Hemostatics - pharmacology Hyperplasia Medical sciences Research Paper Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases T-Lymphocytes - immunology Tunica Intima - immunology Tunica Intima - pathology Vascular surgery: aorta, extremities, vena cava. Surgery of the lymphatic vessels Vasculitis - pathology |
| title | Perivascular Endothelial Implants Inhibit Intimal Hyperplasia in a Model of Arteriovenous Fistulae: A Safety and Efficacy Study in the Pig |
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