Magnetically steerable Fe3O4@Ni2+-NTA-polystyrene nanoparticles for the immobilization and separation of his6-protein
[Display omitted] •Ni2+-NTA-PS NPs encapsulated with magnetic Fe3O4 NPs were synthesized.•The morphology, size and encapsulation were confirmed using SEM, TEM and DLS.•The application of our system was proved by magnetic separation of his6-tag protein. We developed Ni2+-nitrilotriacetic acid (NTA) f...
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| Veröffentlicht in: | European polymer journal 2019-03, Vol.112, p.524-529 |
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| container_title | European polymer journal |
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| creator | Jose, Leeja Lee, Chaeyeon Hwang, Aran Park, Ji Hyun Song, Jae Kwang Paik, Hyun-jong |
| description | [Display omitted]
•Ni2+-NTA-PS NPs encapsulated with magnetic Fe3O4 NPs were synthesized.•The morphology, size and encapsulation were confirmed using SEM, TEM and DLS.•The application of our system was proved by magnetic separation of his6-tag protein.
We developed Ni2+-nitrilotriacetic acid (NTA) functionalized polystyrene (Ni2+-NTA-PS) nanoparticles encapsulated with magnetic Fe3O4 nanoparticles (Fe3O4@Ni2+-NTA-PS nanoparticles) via miniemulsion/solvent evaporation method for the affinity purification of his6-tag recombinant proteins. Fe3O4@Ni2+-NTA-PS nanoparticles have a high surface to volume ratio, which enhanced the protein immobilization efficiency through the specific interaction between his6-tag of protein and Ni2+-NTA end of the polymer. The potential of Fe3O4@Ni2+-NTA-PS nanoparticles is demonstrated by a direct comparison with commercially available Ni2+-NTA-magnetic beads in his6-protein immobilization and recovery. Furthermore, the practical application of Fe3O4@Ni2+-NTA-PS magnetic nanoparticles is illustrated in his6-protein separation from crude cell lysate. |
| doi_str_mv | 10.1016/j.eurpolymj.2019.01.024 |
| format | Article |
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•Ni2+-NTA-PS NPs encapsulated with magnetic Fe3O4 NPs were synthesized.•The morphology, size and encapsulation were confirmed using SEM, TEM and DLS.•The application of our system was proved by magnetic separation of his6-tag protein.
We developed Ni2+-nitrilotriacetic acid (NTA) functionalized polystyrene (Ni2+-NTA-PS) nanoparticles encapsulated with magnetic Fe3O4 nanoparticles (Fe3O4@Ni2+-NTA-PS nanoparticles) via miniemulsion/solvent evaporation method for the affinity purification of his6-tag recombinant proteins. Fe3O4@Ni2+-NTA-PS nanoparticles have a high surface to volume ratio, which enhanced the protein immobilization efficiency through the specific interaction between his6-tag of protein and Ni2+-NTA end of the polymer. The potential of Fe3O4@Ni2+-NTA-PS nanoparticles is demonstrated by a direct comparison with commercially available Ni2+-NTA-magnetic beads in his6-protein immobilization and recovery. Furthermore, the practical application of Fe3O4@Ni2+-NTA-PS magnetic nanoparticles is illustrated in his6-protein separation from crude cell lysate.</description><identifier>ISSN: 0014-3057</identifier><identifier>EISSN: 1873-1945</identifier><identifier>DOI: 10.1016/j.eurpolymj.2019.01.024</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Beads ; Cell lysate purification ; Fe3O4 encapsulation ; Functionalized polystyrene ; His6-protein ; Immobilization ; Iron oxides ; Luminescence ; Magnetic fields ; Miniemulsion ; Nanoparticles ; Ni2+-NTA ; Nitrilotriacetic acid ; Polystyrene ; Polystyrene resins ; Proteins ; Separation</subject><ispartof>European polymer journal, 2019-03, Vol.112, p.524-529</ispartof><rights>2019 Elsevier Ltd</rights><rights>Copyright Elsevier BV Mar 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1887-d13735d82e0590464bd8a27854d5f17c111516b36aeddb2435bc32a7aa1d74283</citedby><cites>FETCH-LOGICAL-c1887-d13735d82e0590464bd8a27854d5f17c111516b36aeddb2435bc32a7aa1d74283</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.eurpolymj.2019.01.024$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids></links><search><creatorcontrib>Jose, Leeja</creatorcontrib><creatorcontrib>Lee, Chaeyeon</creatorcontrib><creatorcontrib>Hwang, Aran</creatorcontrib><creatorcontrib>Park, Ji Hyun</creatorcontrib><creatorcontrib>Song, Jae Kwang</creatorcontrib><creatorcontrib>Paik, Hyun-jong</creatorcontrib><title>Magnetically steerable Fe3O4@Ni2+-NTA-polystyrene nanoparticles for the immobilization and separation of his6-protein</title><title>European polymer journal</title><description>[Display omitted]
•Ni2+-NTA-PS NPs encapsulated with magnetic Fe3O4 NPs were synthesized.•The morphology, size and encapsulation were confirmed using SEM, TEM and DLS.•The application of our system was proved by magnetic separation of his6-tag protein.
We developed Ni2+-nitrilotriacetic acid (NTA) functionalized polystyrene (Ni2+-NTA-PS) nanoparticles encapsulated with magnetic Fe3O4 nanoparticles (Fe3O4@Ni2+-NTA-PS nanoparticles) via miniemulsion/solvent evaporation method for the affinity purification of his6-tag recombinant proteins. Fe3O4@Ni2+-NTA-PS nanoparticles have a high surface to volume ratio, which enhanced the protein immobilization efficiency through the specific interaction between his6-tag of protein and Ni2+-NTA end of the polymer. The potential of Fe3O4@Ni2+-NTA-PS nanoparticles is demonstrated by a direct comparison with commercially available Ni2+-NTA-magnetic beads in his6-protein immobilization and recovery. Furthermore, the practical application of Fe3O4@Ni2+-NTA-PS magnetic nanoparticles is illustrated in his6-protein separation from crude cell lysate.</description><subject>Beads</subject><subject>Cell lysate purification</subject><subject>Fe3O4 encapsulation</subject><subject>Functionalized polystyrene</subject><subject>His6-protein</subject><subject>Immobilization</subject><subject>Iron oxides</subject><subject>Luminescence</subject><subject>Magnetic fields</subject><subject>Miniemulsion</subject><subject>Nanoparticles</subject><subject>Ni2+-NTA</subject><subject>Nitrilotriacetic acid</subject><subject>Polystyrene</subject><subject>Polystyrene resins</subject><subject>Proteins</subject><subject>Separation</subject><issn>0014-3057</issn><issn>1873-1945</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNqFkMtKxDAUhoMoOI4-gwGX0pqTpE27cxBv4GWj65A2p05KJxmTjjA-vR1G3Lo6_PBfOB8h58ByYFBe9Tlu4joM21WfcwZ1ziBnXB6QGVRKZFDL4pDMGAOZCVaoY3KSUs8YU6IUM7J5Nh8eR9eaYdjSNCJG0wxI71C8yusXxy-zl7dFtutP4zaiR-qND2sTp8yAiXYh0nGJ1K1WoXGD-zajC54ab2nCybaXoaNLl8psHcOIzp-So84MCc9-75y8392-3TxkT6_3jzeLp6yFqlKZBaFEYSuOrKiZLGVjK8NVVUhbdKBaACigbERp0NqGS1E0reBGGQNWSV6JObnY9067nxtMo-7DJvppUnOo65rtiEwutXe1MaQUsdPr6FYmbjUwvWOse_3HWO8YawZ6YjwlF_skTk98OYw6tQ59i9ZFbEdtg_u34wc8pYqG</recordid><startdate>201903</startdate><enddate>201903</enddate><creator>Jose, Leeja</creator><creator>Lee, Chaeyeon</creator><creator>Hwang, Aran</creator><creator>Park, Ji Hyun</creator><creator>Song, Jae Kwang</creator><creator>Paik, Hyun-jong</creator><general>Elsevier Ltd</general><general>Elsevier BV</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8FD</scope><scope>JG9</scope></search><sort><creationdate>201903</creationdate><title>Magnetically steerable Fe3O4@Ni2+-NTA-polystyrene nanoparticles for the immobilization and separation of his6-protein</title><author>Jose, Leeja ; Lee, Chaeyeon ; Hwang, Aran ; Park, Ji Hyun ; Song, Jae Kwang ; Paik, Hyun-jong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1887-d13735d82e0590464bd8a27854d5f17c111516b36aeddb2435bc32a7aa1d74283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Beads</topic><topic>Cell lysate purification</topic><topic>Fe3O4 encapsulation</topic><topic>Functionalized polystyrene</topic><topic>His6-protein</topic><topic>Immobilization</topic><topic>Iron oxides</topic><topic>Luminescence</topic><topic>Magnetic fields</topic><topic>Miniemulsion</topic><topic>Nanoparticles</topic><topic>Ni2+-NTA</topic><topic>Nitrilotriacetic acid</topic><topic>Polystyrene</topic><topic>Polystyrene resins</topic><topic>Proteins</topic><topic>Separation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jose, Leeja</creatorcontrib><creatorcontrib>Lee, Chaeyeon</creatorcontrib><creatorcontrib>Hwang, Aran</creatorcontrib><creatorcontrib>Park, Ji Hyun</creatorcontrib><creatorcontrib>Song, Jae Kwang</creatorcontrib><creatorcontrib>Paik, Hyun-jong</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><jtitle>European polymer journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jose, Leeja</au><au>Lee, Chaeyeon</au><au>Hwang, Aran</au><au>Park, Ji Hyun</au><au>Song, Jae Kwang</au><au>Paik, Hyun-jong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Magnetically steerable Fe3O4@Ni2+-NTA-polystyrene nanoparticles for the immobilization and separation of his6-protein</atitle><jtitle>European polymer journal</jtitle><date>2019-03</date><risdate>2019</risdate><volume>112</volume><spage>524</spage><epage>529</epage><pages>524-529</pages><issn>0014-3057</issn><eissn>1873-1945</eissn><abstract>[Display omitted]
•Ni2+-NTA-PS NPs encapsulated with magnetic Fe3O4 NPs were synthesized.•The morphology, size and encapsulation were confirmed using SEM, TEM and DLS.•The application of our system was proved by magnetic separation of his6-tag protein.
We developed Ni2+-nitrilotriacetic acid (NTA) functionalized polystyrene (Ni2+-NTA-PS) nanoparticles encapsulated with magnetic Fe3O4 nanoparticles (Fe3O4@Ni2+-NTA-PS nanoparticles) via miniemulsion/solvent evaporation method for the affinity purification of his6-tag recombinant proteins. Fe3O4@Ni2+-NTA-PS nanoparticles have a high surface to volume ratio, which enhanced the protein immobilization efficiency through the specific interaction between his6-tag of protein and Ni2+-NTA end of the polymer. The potential of Fe3O4@Ni2+-NTA-PS nanoparticles is demonstrated by a direct comparison with commercially available Ni2+-NTA-magnetic beads in his6-protein immobilization and recovery. Furthermore, the practical application of Fe3O4@Ni2+-NTA-PS magnetic nanoparticles is illustrated in his6-protein separation from crude cell lysate.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><doi>10.1016/j.eurpolymj.2019.01.024</doi><tpages>6</tpages></addata></record> |
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| ispartof | European polymer journal, 2019-03, Vol.112, p.524-529 |
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| subjects | Beads Cell lysate purification Fe3O4 encapsulation Functionalized polystyrene His6-protein Immobilization Iron oxides Luminescence Magnetic fields Miniemulsion Nanoparticles Ni2+-NTA Nitrilotriacetic acid Polystyrene Polystyrene resins Proteins Separation |
| title | Magnetically steerable Fe3O4@Ni2+-NTA-polystyrene nanoparticles for the immobilization and separation of his6-protein |
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