Seed yield in red clover is associated with meiotic abnormalities and in tetraploid genotypes also with self-compatibility
Red clover ( Trifolium pratense L.) is a major forage legume crop with diploid and tetraploid cultivars. Seed yield is an important trait in red clover, but the underlying reasons for low seed yield in individual genotypes are not well known. Here we investigated direct associations between seed yie...
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| Veröffentlicht in: | Euphytica 2019-04, Vol.215 (4), p.1-13, Article 79 |
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| creator | Vleugels, Tim Laere, Katrijn Van Roldán-Ruiz, Isabel Cnops, Gerda |
| description | Red clover (
Trifolium pratense
L.) is a major forage legume crop with diploid and tetraploid cultivars. Seed yield is an important trait in red clover, but the underlying reasons for low seed yield in individual genotypes are not well known. Here we investigated direct associations between seed yield and ovule abortion, male meiotic abnormalities, and pollen quality in well-characterized, diploid and tetraploid red clover genotypes with contrasting seed yields. Ovule development was studied after manual pollinations: in most genotypes, high rates of embryo abortion occurred between 7 and 14 days after pollination. However, the frequencies of developing, underdeveloped and shrivelled ovules were similar in diploid and tetraploid genotypes and were not associated with seed yield. Surprisingly, self-pollination only took place in tetraploid genotypes with high seed yield (83% self-pollination on average). The frequencies of meiotic abnormalities during microsporogenesis were investigated, in addition to pollen viability and pollen germination capacity. Tetraploid genotypes displayed more meiotic abnormalities (17.3% and 7.0% respectively) and lower pollen germination (39% and 63% respectively) than diploids. A negative trend was present between meiotic abnormalities and seed yield. In conclusion, our study reveals that high seed yield was associated with a low degree of abnormalities in male meiosis in diploid and tetraploid genotypes, and with the ability to self-pollinate in the tetraploid genotypes investigated. In certain genotypes, defective male meiosis may be a primary cause of low pollen quality and low seed yield. |
| doi_str_mv | 10.1007/s10681-019-2405-6 |
| format | Article |
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Trifolium pratense
L.) is a major forage legume crop with diploid and tetraploid cultivars. Seed yield is an important trait in red clover, but the underlying reasons for low seed yield in individual genotypes are not well known. Here we investigated direct associations between seed yield and ovule abortion, male meiotic abnormalities, and pollen quality in well-characterized, diploid and tetraploid red clover genotypes with contrasting seed yields. Ovule development was studied after manual pollinations: in most genotypes, high rates of embryo abortion occurred between 7 and 14 days after pollination. However, the frequencies of developing, underdeveloped and shrivelled ovules were similar in diploid and tetraploid genotypes and were not associated with seed yield. Surprisingly, self-pollination only took place in tetraploid genotypes with high seed yield (83% self-pollination on average). The frequencies of meiotic abnormalities during microsporogenesis were investigated, in addition to pollen viability and pollen germination capacity. Tetraploid genotypes displayed more meiotic abnormalities (17.3% and 7.0% respectively) and lower pollen germination (39% and 63% respectively) than diploids. A negative trend was present between meiotic abnormalities and seed yield. In conclusion, our study reveals that high seed yield was associated with a low degree of abnormalities in male meiosis in diploid and tetraploid genotypes, and with the ability to self-pollinate in the tetraploid genotypes investigated. In certain genotypes, defective male meiosis may be a primary cause of low pollen quality and low seed yield.</description><identifier>ISSN: 0014-2336</identifier><identifier>EISSN: 1573-5060</identifier><identifier>DOI: 10.1007/s10681-019-2405-6</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Abnormalities ; Abortion ; Beans ; Biomedical and Life Sciences ; Biotechnology ; Clover ; Crop yield ; Cultivars ; Dietary fiber ; Diploids ; Embryos ; Forage crops ; Genetic aspects ; Genotype ; Genotypes ; Germination ; Legumes ; Life Sciences ; Meiosis ; Mimosaceae ; Ovules ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant reproduction ; Plant Sciences ; Pollen ; Pollination ; Red clover ; Seeds ; Trifolium pratense ; Viability</subject><ispartof>Euphytica, 2019-04, Vol.215 (4), p.1-13, Article 79</ispartof><rights>Springer Nature B.V. 2019</rights><rights>COPYRIGHT 2019 Springer</rights><rights>Euphytica is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c355t-66e6839aef42494f1363ccc94e0ed6a23d689aa17cbe591e66ad98d063b969463</citedby><cites>FETCH-LOGICAL-c355t-66e6839aef42494f1363ccc94e0ed6a23d689aa17cbe591e66ad98d063b969463</cites><orcidid>0000-0001-6922-3251</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10681-019-2405-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10681-019-2405-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Vleugels, Tim</creatorcontrib><creatorcontrib>Laere, Katrijn Van</creatorcontrib><creatorcontrib>Roldán-Ruiz, Isabel</creatorcontrib><creatorcontrib>Cnops, Gerda</creatorcontrib><title>Seed yield in red clover is associated with meiotic abnormalities and in tetraploid genotypes also with self-compatibility</title><title>Euphytica</title><addtitle>Euphytica</addtitle><description>Red clover (
Trifolium pratense
L.) is a major forage legume crop with diploid and tetraploid cultivars. Seed yield is an important trait in red clover, but the underlying reasons for low seed yield in individual genotypes are not well known. Here we investigated direct associations between seed yield and ovule abortion, male meiotic abnormalities, and pollen quality in well-characterized, diploid and tetraploid red clover genotypes with contrasting seed yields. Ovule development was studied after manual pollinations: in most genotypes, high rates of embryo abortion occurred between 7 and 14 days after pollination. However, the frequencies of developing, underdeveloped and shrivelled ovules were similar in diploid and tetraploid genotypes and were not associated with seed yield. Surprisingly, self-pollination only took place in tetraploid genotypes with high seed yield (83% self-pollination on average). The frequencies of meiotic abnormalities during microsporogenesis were investigated, in addition to pollen viability and pollen germination capacity. Tetraploid genotypes displayed more meiotic abnormalities (17.3% and 7.0% respectively) and lower pollen germination (39% and 63% respectively) than diploids. A negative trend was present between meiotic abnormalities and seed yield. In conclusion, our study reveals that high seed yield was associated with a low degree of abnormalities in male meiosis in diploid and tetraploid genotypes, and with the ability to self-pollinate in the tetraploid genotypes investigated. In certain genotypes, defective male meiosis may be a primary cause of low pollen quality and low seed yield.</description><subject>Abnormalities</subject><subject>Abortion</subject><subject>Beans</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Clover</subject><subject>Crop yield</subject><subject>Cultivars</subject><subject>Dietary fiber</subject><subject>Diploids</subject><subject>Embryos</subject><subject>Forage crops</subject><subject>Genetic aspects</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>Germination</subject><subject>Legumes</subject><subject>Life Sciences</subject><subject>Meiosis</subject><subject>Mimosaceae</subject><subject>Ovules</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant reproduction</subject><subject>Plant Sciences</subject><subject>Pollen</subject><subject>Pollination</subject><subject>Red clover</subject><subject>Seeds</subject><subject>Trifolium pratense</subject><subject>Viability</subject><issn>0014-2336</issn><issn>1573-5060</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp1kU9LxDAQxYMouK5-AG8Fz9FJ06bNcVn8Bwse1HPIptM1S9vUJKusn96sFTzJHMK8eb9kwiPkksE1A6huAgNRMwpM0ryAkoojMmNlxWkJAo7JDIAVNOdcnJKzELYAIKsSZuTrGbHJ9ha7JrND5lNjOveBPrMh0yE4Y3VM4qeNb1mP1kVrMr0enO91Z6PF5Bp-0IjR67Fztsk2OLi4Hw-zLriJDdi11Lh-1NGubUL35-SkTXO8-D3n5PXu9mX5QFdP94_LxYoaXpaRCoGi5lJjW-SFLFrGBTfGyAIBG6Fz3ohaas0qs8ZSMhRCN7JuQPC1FLIQfE6upntH7953GKLaup0f0pMqZ7LmNRPAk-t6cm10h8oOrUvfMaka7K1xA7Y26YuKFVBxWdYJYBNgvAvBY6tGb3vt94qBOmSipkxUykQdMlGHVfKJCck7bND_rfI_9A1qa5BV</recordid><startdate>20190401</startdate><enddate>20190401</enddate><creator>Vleugels, Tim</creator><creator>Laere, Katrijn Van</creator><creator>Roldán-Ruiz, Isabel</creator><creator>Cnops, Gerda</creator><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SN</scope><scope>7SS</scope><scope>7T7</scope><scope>7TM</scope><scope>7X2</scope><scope>7XB</scope><scope>88I</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>M0K</scope><scope>M2P</scope><scope>M7N</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0001-6922-3251</orcidid></search><sort><creationdate>20190401</creationdate><title>Seed yield in red clover is associated with meiotic abnormalities and in tetraploid genotypes also with self-compatibility</title><author>Vleugels, Tim ; Laere, Katrijn Van ; Roldán-Ruiz, Isabel ; Cnops, Gerda</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c355t-66e6839aef42494f1363ccc94e0ed6a23d689aa17cbe591e66ad98d063b969463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Abnormalities</topic><topic>Abortion</topic><topic>Beans</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Clover</topic><topic>Crop yield</topic><topic>Cultivars</topic><topic>Dietary fiber</topic><topic>Diploids</topic><topic>Embryos</topic><topic>Forage crops</topic><topic>Genetic aspects</topic><topic>Genotype</topic><topic>Genotypes</topic><topic>Germination</topic><topic>Legumes</topic><topic>Life Sciences</topic><topic>Meiosis</topic><topic>Mimosaceae</topic><topic>Ovules</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant reproduction</topic><topic>Plant Sciences</topic><topic>Pollen</topic><topic>Pollination</topic><topic>Red clover</topic><topic>Seeds</topic><topic>Trifolium pratense</topic><topic>Viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vleugels, Tim</creatorcontrib><creatorcontrib>Laere, Katrijn Van</creatorcontrib><creatorcontrib>Roldán-Ruiz, Isabel</creatorcontrib><creatorcontrib>Cnops, Gerda</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Science Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Agricultural Science Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><jtitle>Euphytica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vleugels, Tim</au><au>Laere, Katrijn Van</au><au>Roldán-Ruiz, Isabel</au><au>Cnops, Gerda</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Seed yield in red clover is associated with meiotic abnormalities and in tetraploid genotypes also with self-compatibility</atitle><jtitle>Euphytica</jtitle><stitle>Euphytica</stitle><date>2019-04-01</date><risdate>2019</risdate><volume>215</volume><issue>4</issue><spage>1</spage><epage>13</epage><pages>1-13</pages><artnum>79</artnum><issn>0014-2336</issn><eissn>1573-5060</eissn><abstract>Red clover (
Trifolium pratense
L.) is a major forage legume crop with diploid and tetraploid cultivars. Seed yield is an important trait in red clover, but the underlying reasons for low seed yield in individual genotypes are not well known. Here we investigated direct associations between seed yield and ovule abortion, male meiotic abnormalities, and pollen quality in well-characterized, diploid and tetraploid red clover genotypes with contrasting seed yields. Ovule development was studied after manual pollinations: in most genotypes, high rates of embryo abortion occurred between 7 and 14 days after pollination. However, the frequencies of developing, underdeveloped and shrivelled ovules were similar in diploid and tetraploid genotypes and were not associated with seed yield. Surprisingly, self-pollination only took place in tetraploid genotypes with high seed yield (83% self-pollination on average). The frequencies of meiotic abnormalities during microsporogenesis were investigated, in addition to pollen viability and pollen germination capacity. Tetraploid genotypes displayed more meiotic abnormalities (17.3% and 7.0% respectively) and lower pollen germination (39% and 63% respectively) than diploids. A negative trend was present between meiotic abnormalities and seed yield. In conclusion, our study reveals that high seed yield was associated with a low degree of abnormalities in male meiosis in diploid and tetraploid genotypes, and with the ability to self-pollinate in the tetraploid genotypes investigated. In certain genotypes, defective male meiosis may be a primary cause of low pollen quality and low seed yield.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s10681-019-2405-6</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0001-6922-3251</orcidid></addata></record> |
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| ispartof | Euphytica, 2019-04, Vol.215 (4), p.1-13, Article 79 |
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| subjects | Abnormalities Abortion Beans Biomedical and Life Sciences Biotechnology Clover Crop yield Cultivars Dietary fiber Diploids Embryos Forage crops Genetic aspects Genotype Genotypes Germination Legumes Life Sciences Meiosis Mimosaceae Ovules Plant Genetics and Genomics Plant Pathology Plant Physiology Plant reproduction Plant Sciences Pollen Pollination Red clover Seeds Trifolium pratense Viability |
| title | Seed yield in red clover is associated with meiotic abnormalities and in tetraploid genotypes also with self-compatibility |
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