Protocol for eukaryotic plasma membrane isolation using superparamagnetic nanoparticles
•Phospholipid functionalized Fe3O4 SPMNPs are synthesized for subcellular fractionation.•We report a simplified protocol for SPMNP based Plasma membrane fractionation.•Cationic silica beads with SPMNPs can contribute for plasma membrane fractionation. Superparamagnetic nanoparticles (SPMNP) are used...
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| Veröffentlicht in: | Journal of magnetism and magnetic materials 2019-04, Vol.476, p.628-631 |
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| container_title | Journal of magnetism and magnetic materials |
| container_volume | 476 |
| creator | Thimiri Govinda Raj, Deepak B. Khan, Niamat Ali |
| description | •Phospholipid functionalized Fe3O4 SPMNPs are synthesized for subcellular fractionation.•We report a simplified protocol for SPMNP based Plasma membrane fractionation.•Cationic silica beads with SPMNPs can contribute for plasma membrane fractionation.
Superparamagnetic nanoparticles (SPMNP) are used in cell biology for various applications such as subcellular fractionation and for high pure organelle isolation. We simplified previously established methodology with the use of SPMNP for isolation of high pure and high yield plasma membrane from adherent eukaryotic cell line (Thimiri Govinda Raj et al., 2011, 2012, 2016; Tharkeshwar et al., 2014). Here, we show a step by step protocol for SPMNP based plasma membrane fractionation. Our research thus could be used as an alternative method to isolate plasma membrane fraction for cell biology, system biology and immunology research. |
| doi_str_mv | 10.1016/j.jmmm.2017.12.070 |
| format | Article |
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Superparamagnetic nanoparticles (SPMNP) are used in cell biology for various applications such as subcellular fractionation and for high pure organelle isolation. We simplified previously established methodology with the use of SPMNP for isolation of high pure and high yield plasma membrane from adherent eukaryotic cell line (Thimiri Govinda Raj et al., 2011, 2012, 2016; Tharkeshwar et al., 2014). Here, we show a step by step protocol for SPMNP based plasma membrane fractionation. Our research thus could be used as an alternative method to isolate plasma membrane fraction for cell biology, system biology and immunology research.</description><identifier>ISSN: 0304-8853</identifier><identifier>EISSN: 1873-4766</identifier><identifier>DOI: 10.1016/j.jmmm.2017.12.070</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Biology ; Cationic silica based fractionation ; Endosomes ; Fractionation ; Immunology ; Lysosomes ; Nanoparticles ; Plasma ; Plasma membrane ; Superparamagnetic nanoparticles (SPMNP)</subject><ispartof>Journal of magnetism and magnetic materials, 2019-04, Vol.476, p.628-631</ispartof><rights>2017 Elsevier B.V.</rights><rights>Copyright Elsevier BV Apr 15, 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-b3ecbb37f9e8e4760aadd3e4aeb09c093a132988ea1a41d02cfb89b648b10bc63</citedby><cites>FETCH-LOGICAL-c365t-b3ecbb37f9e8e4760aadd3e4aeb09c093a132988ea1a41d02cfb89b648b10bc63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0304885317325568$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids></links><search><creatorcontrib>Thimiri Govinda Raj, Deepak B.</creatorcontrib><creatorcontrib>Khan, Niamat Ali</creatorcontrib><title>Protocol for eukaryotic plasma membrane isolation using superparamagnetic nanoparticles</title><title>Journal of magnetism and magnetic materials</title><description>•Phospholipid functionalized Fe3O4 SPMNPs are synthesized for subcellular fractionation.•We report a simplified protocol for SPMNP based Plasma membrane fractionation.•Cationic silica beads with SPMNPs can contribute for plasma membrane fractionation.
Superparamagnetic nanoparticles (SPMNP) are used in cell biology for various applications such as subcellular fractionation and for high pure organelle isolation. We simplified previously established methodology with the use of SPMNP for isolation of high pure and high yield plasma membrane from adherent eukaryotic cell line (Thimiri Govinda Raj et al., 2011, 2012, 2016; Tharkeshwar et al., 2014). Here, we show a step by step protocol for SPMNP based plasma membrane fractionation. Our research thus could be used as an alternative method to isolate plasma membrane fraction for cell biology, system biology and immunology research.</description><subject>Biology</subject><subject>Cationic silica based fractionation</subject><subject>Endosomes</subject><subject>Fractionation</subject><subject>Immunology</subject><subject>Lysosomes</subject><subject>Nanoparticles</subject><subject>Plasma</subject><subject>Plasma membrane</subject><subject>Superparamagnetic nanoparticles (SPMNP)</subject><issn>0304-8853</issn><issn>1873-4766</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LxDAQhoMouH78AU8Fz62Tppum4EUWv2BBD4rHkKTTJbVpatIK_nuzrGdPMwzvO_POQ8gVhYIC5Td90TvnihJoXdCygBqOyIqKmuVVzfkxWQGDKhdizU7JWYw9ANBK8BX5eA1-9sYPWedDhsunCj9-tiabBhWdyhw6HdSImY1-ULP1Y7ZEO-6yuEwYJhWUU7sR945RjT4NUjtgvCAnnRoiXv7Vc_L-cP-2ecq3L4_Pm7ttbhhfz7lmaLRmddegwBQVlGpbhpVCDY2BhinKykYIVFRVtIXSdFo0mldCU9CGs3Nyfdg7Bf-1YJxl75cwppOypE0tBGVcJFV5UJngYwzYySlYl16VFOQeoOzlHqDcA5S0lAlgMt0eTJjyf1sMMhqLo8HWBjSzbL39z_4LSbF8Ww</recordid><startdate>20190415</startdate><enddate>20190415</enddate><creator>Thimiri Govinda Raj, Deepak B.</creator><creator>Khan, Niamat Ali</creator><general>Elsevier B.V</general><general>Elsevier BV</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20190415</creationdate><title>Protocol for eukaryotic plasma membrane isolation using superparamagnetic nanoparticles</title><author>Thimiri Govinda Raj, Deepak B. ; Khan, Niamat Ali</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-b3ecbb37f9e8e4760aadd3e4aeb09c093a132988ea1a41d02cfb89b648b10bc63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Biology</topic><topic>Cationic silica based fractionation</topic><topic>Endosomes</topic><topic>Fractionation</topic><topic>Immunology</topic><topic>Lysosomes</topic><topic>Nanoparticles</topic><topic>Plasma</topic><topic>Plasma membrane</topic><topic>Superparamagnetic nanoparticles (SPMNP)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thimiri Govinda Raj, Deepak B.</creatorcontrib><creatorcontrib>Khan, Niamat Ali</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Journal of magnetism and magnetic materials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thimiri Govinda Raj, Deepak B.</au><au>Khan, Niamat Ali</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protocol for eukaryotic plasma membrane isolation using superparamagnetic nanoparticles</atitle><jtitle>Journal of magnetism and magnetic materials</jtitle><date>2019-04-15</date><risdate>2019</risdate><volume>476</volume><spage>628</spage><epage>631</epage><pages>628-631</pages><issn>0304-8853</issn><eissn>1873-4766</eissn><abstract>•Phospholipid functionalized Fe3O4 SPMNPs are synthesized for subcellular fractionation.•We report a simplified protocol for SPMNP based Plasma membrane fractionation.•Cationic silica beads with SPMNPs can contribute for plasma membrane fractionation.
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| ispartof | Journal of magnetism and magnetic materials, 2019-04, Vol.476, p.628-631 |
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| language | eng |
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| source | Elsevier ScienceDirect Journals |
| subjects | Biology Cationic silica based fractionation Endosomes Fractionation Immunology Lysosomes Nanoparticles Plasma Plasma membrane Superparamagnetic nanoparticles (SPMNP) |
| title | Protocol for eukaryotic plasma membrane isolation using superparamagnetic nanoparticles |
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