Effect of glucose and palmitate environment on proliferation and migration of PC3‐prostate cancer cells

Recent studies have been trying to find out how diet and metabolic changes such as dyslipidaemia, hyperglycaemia, and hyperinsulinaemia can stimulate cancer progression. This investigation aimed to evaluate the effect of high concentrations of fatty acids and/or glucose in tumour prostate cells, foc...

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Veröffentlicht in:Cell biology international 2019-04, Vol.43 (4), p.373-383
Hauptverfasser: Rezende, Lívia Prometti, Galheigo, Maria Raquel Unterkircher, Landim, Breno Costa, Cruz, Amanda Rodrigues, Botelho, Françoise Vasconcelos, Zanon, Renata Graciele, Góes, Rejane Maira, Ribeiro, Daniele Lisboa
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container_end_page 383
container_issue 4
container_start_page 373
container_title Cell biology international
container_volume 43
creator Rezende, Lívia Prometti
Galheigo, Maria Raquel Unterkircher
Landim, Breno Costa
Cruz, Amanda Rodrigues
Botelho, Françoise Vasconcelos
Zanon, Renata Graciele
Góes, Rejane Maira
Ribeiro, Daniele Lisboa
description Recent studies have been trying to find out how diet and metabolic changes such as dyslipidaemia, hyperglycaemia, and hyperinsulinaemia can stimulate cancer progression. This investigation aimed to evaluate the effect of high concentrations of fatty acids and/or glucose in tumour prostate cells, focusing on the proliferation/migration profile and oxidative stress. PC3 cells were treated with high concentration of saturated fatty acid (palmitate, 100 µM), glucose (220 mg/dL), or both for 24 or 48 h. Results demonstrated that PC3 cells showed a significant increase in proliferation after 48 h of treatment with glucose and palmitate+glucose. Cell proliferation was associated with reduced levels of AMPK phosphorylation in glucose group at 24 and 48 h of treatment, while palmitate group presented this result only after 48 h of treatment. Also, there was a significant increase in cell migration between time 0 and 48 h after all treatments, except in the control. Catalase activity was increased by palmitate in the beginning of treatment, while glucose presented a later effect. Also, nitrite production was increased by glucose only after 48 h, and the total antioxidant activity was enhanced by palmitate in the initial hours. Thus, we conclude that the high concentration of the saturated fatty acid palmitate and glucose in vitro influences PC3 cells and stimulates cellular activities related to carcinogenesis such as cell proliferation, migration, and oxidative stress in different ways. Palmitate presents a rapid and initial effect, while a glucose environment stimulates cells later on, maintaining high levels of cell proliferation.
doi_str_mv 10.1002/cbin.11066
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This investigation aimed to evaluate the effect of high concentrations of fatty acids and/or glucose in tumour prostate cells, focusing on the proliferation/migration profile and oxidative stress. PC3 cells were treated with high concentration of saturated fatty acid (palmitate, 100 µM), glucose (220 mg/dL), or both for 24 or 48 h. Results demonstrated that PC3 cells showed a significant increase in proliferation after 48 h of treatment with glucose and palmitate+glucose. Cell proliferation was associated with reduced levels of AMPK phosphorylation in glucose group at 24 and 48 h of treatment, while palmitate group presented this result only after 48 h of treatment. Also, there was a significant increase in cell migration between time 0 and 48 h after all treatments, except in the control. Catalase activity was increased by palmitate in the beginning of treatment, while glucose presented a later effect. Also, nitrite production was increased by glucose only after 48 h, and the total antioxidant activity was enhanced by palmitate in the initial hours. Thus, we conclude that the high concentration of the saturated fatty acid palmitate and glucose in vitro influences PC3 cells and stimulates cellular activities related to carcinogenesis such as cell proliferation, migration, and oxidative stress in different ways. 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Also, nitrite production was increased by glucose only after 48 h, and the total antioxidant activity was enhanced by palmitate in the initial hours. Thus, we conclude that the high concentration of the saturated fatty acid palmitate and glucose in vitro influences PC3 cells and stimulates cellular activities related to carcinogenesis such as cell proliferation, migration, and oxidative stress in different ways. Palmitate presents a rapid and initial effect, while a glucose environment stimulates cells later on, maintaining high levels of cell proliferation.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>30353973</pmid><doi>10.1002/cbin.11066</doi><tpages>11</tpages></addata></record>
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subjects AMPK
Antioxidants
Carcinogenesis
Catalase
Cell adhesion & migration
Cell growth
Cell migration
Cell Movement - drug effects
Cell proliferation
Cell Proliferation - drug effects
Dyslipidemia
Fatty acids
Fatty Acids - metabolism
Glucose
Glucose - adverse effects
Glucose - metabolism
Glucose - physiology
Humans
Hyperglycemia
Hyperinsulinism - metabolism
Insulin - metabolism
Male
Oxidative stress
palmitate
Palmitates - metabolism
Palmitates - pharmacology
Palmitic acid
PC-3 Cells - drug effects
Phosphorylation
Prostate - metabolism
Prostate cancer
Prostatic Neoplasms - metabolism
saturated fatty acid
Tumors
title Effect of glucose and palmitate environment on proliferation and migration of PC3‐prostate cancer cells
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