Within- and between-laboratory precision in the measurement of body volume using air displacement plethysmography and its effect on body composition assessment

OBJECTIVE: To determine and compare the extent of within- and between-laboratory precision in body volume (BV) measurements using air displacement plethysmography (ADP), the BOD POD body composition system, and to interpret any such variability in terms of body composition estimates. DESIGN: Repeate...

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Veröffentlicht in:International Journal of Obesity 2004-01, Vol.28 (1), p.80-90
Hauptverfasser: Collins, A L, Saunders, S, McCarthy, H D, Williams, J E, Fuller, N J
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creator Collins, A L
Saunders, S
McCarthy, H D
Williams, J E
Fuller, N J
description OBJECTIVE: To determine and compare the extent of within- and between-laboratory precision in body volume (BV) measurements using air displacement plethysmography (ADP), the BOD POD body composition system, and to interpret any such variability in terms of body composition estimates. DESIGN: Repeated test procedures of BV assessment using the BOD POD ADP were reproduced at two laboratories for the estimation of precision, both within and between laboratories. SUBJECTS: In total, 30 healthy adult volunteers, 14 men (age, 19–48 y; body mass index (BMI), 19.7–30.3 kg/m 2 ) and 16 women (age, 19–40 y; BMI, 16.3–35.7 kg/m 2 ), were each subjected to two test procedures at both laboratories. Two additional volunteers were independently subjected to 10 repeated test procedures at both laboratories. MEASUREMENTS: Repeated measurements of BV, uncorrected for the effects of isothermal air in the lungs and the surface area artifact, were obtained using the BOD POD ADP, with the identical protocol being faithfully applied at both laboratories. Uncorrected BV measurements were adjusted to give estimates of actual BV that were used to calculate body density (body weight (BWt)/actual BV) from which estimates of body composition were derived. The differences between repeated BV measurements or body composition estimates were used to assess within-laboratory precision (repeatability), as standard deviation (SD) and coefficient of variation; the differences between measurements reproduced at each laboratory were used to determine between-laboratory precision (reproducibility), as bias and 95% limits of agreement (from SD of the differences between laboratories). RESULTS: The extent of within-laboratory methodological precision for BV (uncorrected and actual) was variable according to subject, sample group and laboratory conditions (range of SD, 0.04–0.13 l), and was mostly due to within-individual biological variability (typically 78–99%) rather than to technical imprecision. There was a significant ( P
doi_str_mv 10.1038/sj.ijo.0802466
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DESIGN: Repeated test procedures of BV assessment using the BOD POD ADP were reproduced at two laboratories for the estimation of precision, both within and between laboratories. SUBJECTS: In total, 30 healthy adult volunteers, 14 men (age, 19–48 y; body mass index (BMI), 19.7–30.3 kg/m 2 ) and 16 women (age, 19–40 y; BMI, 16.3–35.7 kg/m 2 ), were each subjected to two test procedures at both laboratories. Two additional volunteers were independently subjected to 10 repeated test procedures at both laboratories. MEASUREMENTS: Repeated measurements of BV, uncorrected for the effects of isothermal air in the lungs and the surface area artifact, were obtained using the BOD POD ADP, with the identical protocol being faithfully applied at both laboratories. Uncorrected BV measurements were adjusted to give estimates of actual BV that were used to calculate body density (body weight (BWt)/actual BV) from which estimates of body composition were derived. The differences between repeated BV measurements or body composition estimates were used to assess within-laboratory precision (repeatability), as standard deviation (SD) and coefficient of variation; the differences between measurements reproduced at each laboratory were used to determine between-laboratory precision (reproducibility), as bias and 95% limits of agreement (from SD of the differences between laboratories). RESULTS: The extent of within-laboratory methodological precision for BV (uncorrected and actual) was variable according to subject, sample group and laboratory conditions (range of SD, 0.04–0.13 l), and was mostly due to within-individual biological variability (typically 78–99%) rather than to technical imprecision. There was a significant ( P &lt;0.05) bias between laboratories for the 10 repeats on the two independent subjects (up to 0.29 l). Although no significant bias ( P =0.077) was evident for the sample group of 30 volunteers (−0.05 l), the 95% limits of agreement were considerable (−0.68 to 0.58 l). The effects of this variability in BV on body composition were relatively greater: for example, within-laboratory precision (SD) for body fat as % BWt was between 0.56 and 1.34% depending on the subject and laboratory; the bias (−0.59%) was not significant between laboratories, but there were large 95% limits of agreement (−3.67 to 2.50%). CONCLUSION: Within-laboratory precision for each BOD POD instrument was reasonably good, but was variable according to the prevailing conditions. Although the bias between the two instruments was not significant for the BV measurements, implying that they can be used interchangeably for groups of similar subjects, the relatively large 95% limits of agreement indicate that greater consideration may be needed for assessing individuals with different ADP instruments. Therefore, use of a single ADP instrument is apparently preferable when assessing individuals on a longitudinal basis.</description><identifier>ISSN: 0307-0565</identifier><identifier>EISSN: 1476-5497</identifier><identifier>DOI: 10.1038/sj.ijo.0802466</identifier><identifier>PMID: 14710169</identifier><identifier>CODEN: IJOBDP</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Adult ; Agreements ; Bias ; Biological and medical sciences ; Body composition ; Body Composition - physiology ; Body Constitution - physiology ; Body fat ; Body mass index ; Body weight ; Coefficient of variation ; Epidemiology ; Estimates ; Female ; Health aspects ; Health Promotion and Disease Prevention ; Humans ; Internal Medicine ; Laboratories ; Laboratories - standards ; Lungs ; Male ; Medical sciences ; Medicine ; Medicine &amp; Public Health ; Metabolic Diseases ; Middle Aged ; Nutrition research ; Obesity ; Physiological aspects ; Plethysmography ; Plethysmography - standards ; Public Health ; Reference Standards ; Reproducibility of Results ; Sensitivity and Specificity</subject><ispartof>International Journal of Obesity, 2004-01, Vol.28 (1), p.80-90</ispartof><rights>Springer Nature Limited 2004</rights><rights>2004 INIST-CNRS</rights><rights>COPYRIGHT 2004 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Jan 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c517t-1a7b7f44c189fe3dae32cc773f09793f6421e1ff2f5157d8878f96374eed0a9b3</citedby><cites>FETCH-LOGICAL-c517t-1a7b7f44c189fe3dae32cc773f09793f6421e1ff2f5157d8878f96374eed0a9b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2727,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=15705958$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14710169$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Collins, A L</creatorcontrib><creatorcontrib>Saunders, S</creatorcontrib><creatorcontrib>McCarthy, H D</creatorcontrib><creatorcontrib>Williams, J E</creatorcontrib><creatorcontrib>Fuller, N J</creatorcontrib><title>Within- and between-laboratory precision in the measurement of body volume using air displacement plethysmography and its effect on body composition assessment</title><title>International Journal of Obesity</title><addtitle>Int J Obes</addtitle><addtitle>Int J Obes Relat Metab Disord</addtitle><description>OBJECTIVE: To determine and compare the extent of within- and between-laboratory precision in body volume (BV) measurements using air displacement plethysmography (ADP), the BOD POD body composition system, and to interpret any such variability in terms of body composition estimates. DESIGN: Repeated test procedures of BV assessment using the BOD POD ADP were reproduced at two laboratories for the estimation of precision, both within and between laboratories. SUBJECTS: In total, 30 healthy adult volunteers, 14 men (age, 19–48 y; body mass index (BMI), 19.7–30.3 kg/m 2 ) and 16 women (age, 19–40 y; BMI, 16.3–35.7 kg/m 2 ), were each subjected to two test procedures at both laboratories. Two additional volunteers were independently subjected to 10 repeated test procedures at both laboratories. MEASUREMENTS: Repeated measurements of BV, uncorrected for the effects of isothermal air in the lungs and the surface area artifact, were obtained using the BOD POD ADP, with the identical protocol being faithfully applied at both laboratories. Uncorrected BV measurements were adjusted to give estimates of actual BV that were used to calculate body density (body weight (BWt)/actual BV) from which estimates of body composition were derived. The differences between repeated BV measurements or body composition estimates were used to assess within-laboratory precision (repeatability), as standard deviation (SD) and coefficient of variation; the differences between measurements reproduced at each laboratory were used to determine between-laboratory precision (reproducibility), as bias and 95% limits of agreement (from SD of the differences between laboratories). RESULTS: The extent of within-laboratory methodological precision for BV (uncorrected and actual) was variable according to subject, sample group and laboratory conditions (range of SD, 0.04–0.13 l), and was mostly due to within-individual biological variability (typically 78–99%) rather than to technical imprecision. There was a significant ( P &lt;0.05) bias between laboratories for the 10 repeats on the two independent subjects (up to 0.29 l). Although no significant bias ( P =0.077) was evident for the sample group of 30 volunteers (−0.05 l), the 95% limits of agreement were considerable (−0.68 to 0.58 l). The effects of this variability in BV on body composition were relatively greater: for example, within-laboratory precision (SD) for body fat as % BWt was between 0.56 and 1.34% depending on the subject and laboratory; the bias (−0.59%) was not significant between laboratories, but there were large 95% limits of agreement (−3.67 to 2.50%). CONCLUSION: Within-laboratory precision for each BOD POD instrument was reasonably good, but was variable according to the prevailing conditions. Although the bias between the two instruments was not significant for the BV measurements, implying that they can be used interchangeably for groups of similar subjects, the relatively large 95% limits of agreement indicate that greater consideration may be needed for assessing individuals with different ADP instruments. 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Saunders, S ; McCarthy, H D ; Williams, J E ; Fuller, N J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c517t-1a7b7f44c189fe3dae32cc773f09793f6421e1ff2f5157d8878f96374eed0a9b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adult</topic><topic>Agreements</topic><topic>Bias</topic><topic>Biological and medical sciences</topic><topic>Body composition</topic><topic>Body Composition - physiology</topic><topic>Body Constitution - physiology</topic><topic>Body fat</topic><topic>Body mass index</topic><topic>Body weight</topic><topic>Coefficient of variation</topic><topic>Epidemiology</topic><topic>Estimates</topic><topic>Female</topic><topic>Health aspects</topic><topic>Health Promotion and Disease Prevention</topic><topic>Humans</topic><topic>Internal Medicine</topic><topic>Laboratories</topic><topic>Laboratories - standards</topic><topic>Lungs</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Medicine</topic><topic>Medicine &amp; Public Health</topic><topic>Metabolic Diseases</topic><topic>Middle Aged</topic><topic>Nutrition research</topic><topic>Obesity</topic><topic>Physiological aspects</topic><topic>Plethysmography</topic><topic>Plethysmography - standards</topic><topic>Public Health</topic><topic>Reference Standards</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Collins, A L</creatorcontrib><creatorcontrib>Saunders, S</creatorcontrib><creatorcontrib>McCarthy, H D</creatorcontrib><creatorcontrib>Williams, J E</creatorcontrib><creatorcontrib>Fuller, N J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Physical Education Index</collection><collection>Agricultural Science Collection</collection><collection>Health &amp; 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DESIGN: Repeated test procedures of BV assessment using the BOD POD ADP were reproduced at two laboratories for the estimation of precision, both within and between laboratories. SUBJECTS: In total, 30 healthy adult volunteers, 14 men (age, 19–48 y; body mass index (BMI), 19.7–30.3 kg/m 2 ) and 16 women (age, 19–40 y; BMI, 16.3–35.7 kg/m 2 ), were each subjected to two test procedures at both laboratories. Two additional volunteers were independently subjected to 10 repeated test procedures at both laboratories. MEASUREMENTS: Repeated measurements of BV, uncorrected for the effects of isothermal air in the lungs and the surface area artifact, were obtained using the BOD POD ADP, with the identical protocol being faithfully applied at both laboratories. Uncorrected BV measurements were adjusted to give estimates of actual BV that were used to calculate body density (body weight (BWt)/actual BV) from which estimates of body composition were derived. The differences between repeated BV measurements or body composition estimates were used to assess within-laboratory precision (repeatability), as standard deviation (SD) and coefficient of variation; the differences between measurements reproduced at each laboratory were used to determine between-laboratory precision (reproducibility), as bias and 95% limits of agreement (from SD of the differences between laboratories). RESULTS: The extent of within-laboratory methodological precision for BV (uncorrected and actual) was variable according to subject, sample group and laboratory conditions (range of SD, 0.04–0.13 l), and was mostly due to within-individual biological variability (typically 78–99%) rather than to technical imprecision. There was a significant ( P &lt;0.05) bias between laboratories for the 10 repeats on the two independent subjects (up to 0.29 l). Although no significant bias ( P =0.077) was evident for the sample group of 30 volunteers (−0.05 l), the 95% limits of agreement were considerable (−0.68 to 0.58 l). The effects of this variability in BV on body composition were relatively greater: for example, within-laboratory precision (SD) for body fat as % BWt was between 0.56 and 1.34% depending on the subject and laboratory; the bias (−0.59%) was not significant between laboratories, but there were large 95% limits of agreement (−3.67 to 2.50%). CONCLUSION: Within-laboratory precision for each BOD POD instrument was reasonably good, but was variable according to the prevailing conditions. Although the bias between the two instruments was not significant for the BV measurements, implying that they can be used interchangeably for groups of similar subjects, the relatively large 95% limits of agreement indicate that greater consideration may be needed for assessing individuals with different ADP instruments. Therefore, use of a single ADP instrument is apparently preferable when assessing individuals on a longitudinal basis.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>14710169</pmid><doi>10.1038/sj.ijo.0802466</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record>
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subjects Adult
Agreements
Bias
Biological and medical sciences
Body composition
Body Composition - physiology
Body Constitution - physiology
Body fat
Body mass index
Body weight
Coefficient of variation
Epidemiology
Estimates
Female
Health aspects
Health Promotion and Disease Prevention
Humans
Internal Medicine
Laboratories
Laboratories - standards
Lungs
Male
Medical sciences
Medicine
Medicine & Public Health
Metabolic Diseases
Middle Aged
Nutrition research
Obesity
Physiological aspects
Plethysmography
Plethysmography - standards
Public Health
Reference Standards
Reproducibility of Results
Sensitivity and Specificity
title Within- and between-laboratory precision in the measurement of body volume using air displacement plethysmography and its effect on body composition assessment
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