Downregulation of telomerase activity in human promyelocytic cell line using RNA interference
Telomerase is a ribonucleoprotein complex. It consists of two main components, human telomerase reverse transcriptase (hTERT) and human telomerase RNA. High telomerase activity is present in most malignant cells, but it is barely detectable in majority of somatic cells. The direct correlation betwee...
Gespeichert in:
| Veröffentlicht in: | Annals of hematology 2009-12, Vol.88 (12), p.1169-1176 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1176 |
|---|---|
| container_issue | 12 |
| container_start_page | 1169 |
| container_title | Annals of hematology |
| container_volume | 88 |
| creator | Miri-Moghaddam, E Deezagi, A Soheili, Z. S |
| description | Telomerase is a ribonucleoprotein complex. It consists of two main components, human telomerase reverse transcriptase (hTERT) and human telomerase RNA. High telomerase activity is present in most malignant cells, but it is barely detectable in majority of somatic cells. The direct correlation between telomerase reactivation and carcinogens has made hTERT a key target for anticancer therapeutic studies. In this study, for the first time, we evaluated the ability of the new generation of short interfering RNA (siRNA) to regulate telomerase activity in the human promyelocytic leukemia cell line (HL-60). Transient transfection cell line by hTERT siRNAs resulted in statistically significant suppression of hTERT messenger RNAs which were detected by quantitative real-time polymerase chain reaction, while the expressed hTERT protein levels were measured by flow cytometry. The results of telomeric repeat amplification protocol showed that telomerase activity was significantly reduced upon transfection of the HL-60 cell line with hTERT siRNAs. The results of this study showed that telomerase activity and cell proliferation were efficiently inhibited in the hTERT siRNA-treated leukemic cell line. |
| doi_str_mv | 10.1007/s00277-009-0748-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_219212183</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1883464411</sourcerecordid><originalsourceid>FETCH-LOGICAL-c394t-bfba2d52a6e5f7a2ee3a94d7a6e013595841593b5ce64417d7868c4343f5d01d3</originalsourceid><addsrcrecordid>eNp9kEtv1TAQhS0EopfCD2ADFvvAjB9xvKxaXlIFEtAlsnydycVV4hQ7Ad1_j6tcqTtmY438nTMzh7GXCG8RwLwrAMKYBsA2YFTXwCO2QyVFA7pTj9kOrLSNrnXGnpVyC4CiU-IpO0OrUAvT7tjPq_lvynRYR7_EOfF54AuN80TZF-I-LPFPXI48Jv5rnXzid3mejhUIxyUGHmgc-RgT8bXEdODfvlxUdKE8UKYU6Dl7Mvix0IvTe85uPrz_cfmpuf768fPlxXUTpFVLsx_2XvRa-Jb0YLwgkt6q3tQeUGpbr0Ft5V4HapVC05uu7YKSSg66B-zlOXuz-db1fq9UFnc7rznVkU6gFSiwkxXCDQp5LiXT4O5ynHw-OgR3n6fb8nQ1T3efp4OqeXUyXvcT9Q-KU4AVEBtQ6lc6UH6Y_D_X15to8LPzhxyLu_ku6qmArdVKoPwH19aKBA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>219212183</pqid></control><display><type>article</type><title>Downregulation of telomerase activity in human promyelocytic cell line using RNA interference</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Miri-Moghaddam, E ; Deezagi, A ; Soheili, Z. S</creator><creatorcontrib>Miri-Moghaddam, E ; Deezagi, A ; Soheili, Z. S</creatorcontrib><description>Telomerase is a ribonucleoprotein complex. It consists of two main components, human telomerase reverse transcriptase (hTERT) and human telomerase RNA. High telomerase activity is present in most malignant cells, but it is barely detectable in majority of somatic cells. The direct correlation between telomerase reactivation and carcinogens has made hTERT a key target for anticancer therapeutic studies. In this study, for the first time, we evaluated the ability of the new generation of short interfering RNA (siRNA) to regulate telomerase activity in the human promyelocytic leukemia cell line (HL-60). Transient transfection cell line by hTERT siRNAs resulted in statistically significant suppression of hTERT messenger RNAs which were detected by quantitative real-time polymerase chain reaction, while the expressed hTERT protein levels were measured by flow cytometry. The results of telomeric repeat amplification protocol showed that telomerase activity was significantly reduced upon transfection of the HL-60 cell line with hTERT siRNAs. The results of this study showed that telomerase activity and cell proliferation were efficiently inhibited in the hTERT siRNA-treated leukemic cell line.</description><identifier>ISSN: 0939-5555</identifier><identifier>EISSN: 1432-0584</identifier><identifier>DOI: 10.1007/s00277-009-0748-0</identifier><identifier>PMID: 19415276</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Cell Proliferation ; Down-Regulation ; Hematology ; HL-60 Cells - metabolism ; Humans ; Medicine ; Medicine & Public Health ; Oncology ; Original Article ; RNA - genetics ; RNA - metabolism ; RNA Interference ; RNA, Small Interfering - genetics ; RNA, Small Interfering - metabolism ; Telomerase - genetics ; Telomerase - metabolism ; Transfection</subject><ispartof>Annals of hematology, 2009-12, Vol.88 (12), p.1169-1176</ispartof><rights>Springer-Verlag 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c394t-bfba2d52a6e5f7a2ee3a94d7a6e013595841593b5ce64417d7868c4343f5d01d3</citedby><cites>FETCH-LOGICAL-c394t-bfba2d52a6e5f7a2ee3a94d7a6e013595841593b5ce64417d7868c4343f5d01d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00277-009-0748-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00277-009-0748-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19415276$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miri-Moghaddam, E</creatorcontrib><creatorcontrib>Deezagi, A</creatorcontrib><creatorcontrib>Soheili, Z. S</creatorcontrib><title>Downregulation of telomerase activity in human promyelocytic cell line using RNA interference</title><title>Annals of hematology</title><addtitle>Ann Hematol</addtitle><addtitle>Ann Hematol</addtitle><description>Telomerase is a ribonucleoprotein complex. It consists of two main components, human telomerase reverse transcriptase (hTERT) and human telomerase RNA. High telomerase activity is present in most malignant cells, but it is barely detectable in majority of somatic cells. The direct correlation between telomerase reactivation and carcinogens has made hTERT a key target for anticancer therapeutic studies. In this study, for the first time, we evaluated the ability of the new generation of short interfering RNA (siRNA) to regulate telomerase activity in the human promyelocytic leukemia cell line (HL-60). Transient transfection cell line by hTERT siRNAs resulted in statistically significant suppression of hTERT messenger RNAs which were detected by quantitative real-time polymerase chain reaction, while the expressed hTERT protein levels were measured by flow cytometry. The results of telomeric repeat amplification protocol showed that telomerase activity was significantly reduced upon transfection of the HL-60 cell line with hTERT siRNAs. The results of this study showed that telomerase activity and cell proliferation were efficiently inhibited in the hTERT siRNA-treated leukemic cell line.</description><subject>Cell Proliferation</subject><subject>Down-Regulation</subject><subject>Hematology</subject><subject>HL-60 Cells - metabolism</subject><subject>Humans</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Oncology</subject><subject>Original Article</subject><subject>RNA - genetics</subject><subject>RNA - metabolism</subject><subject>RNA Interference</subject><subject>RNA, Small Interfering - genetics</subject><subject>RNA, Small Interfering - metabolism</subject><subject>Telomerase - genetics</subject><subject>Telomerase - metabolism</subject><subject>Transfection</subject><issn>0939-5555</issn><issn>1432-0584</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp9kEtv1TAQhS0EopfCD2ADFvvAjB9xvKxaXlIFEtAlsnydycVV4hQ7Ad1_j6tcqTtmY438nTMzh7GXCG8RwLwrAMKYBsA2YFTXwCO2QyVFA7pTj9kOrLSNrnXGnpVyC4CiU-IpO0OrUAvT7tjPq_lvynRYR7_EOfF54AuN80TZF-I-LPFPXI48Jv5rnXzid3mejhUIxyUGHmgc-RgT8bXEdODfvlxUdKE8UKYU6Dl7Mvix0IvTe85uPrz_cfmpuf768fPlxXUTpFVLsx_2XvRa-Jb0YLwgkt6q3tQeUGpbr0Ft5V4HapVC05uu7YKSSg66B-zlOXuz-db1fq9UFnc7rznVkU6gFSiwkxXCDQp5LiXT4O5ynHw-OgR3n6fb8nQ1T3efp4OqeXUyXvcT9Q-KU4AVEBtQ6lc6UH6Y_D_X15to8LPzhxyLu_ku6qmArdVKoPwH19aKBA</recordid><startdate>20091201</startdate><enddate>20091201</enddate><creator>Miri-Moghaddam, E</creator><creator>Deezagi, A</creator><creator>Soheili, Z. S</creator><general>Berlin/Heidelberg : Springer-Verlag</general><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20091201</creationdate><title>Downregulation of telomerase activity in human promyelocytic cell line using RNA interference</title><author>Miri-Moghaddam, E ; Deezagi, A ; Soheili, Z. S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c394t-bfba2d52a6e5f7a2ee3a94d7a6e013595841593b5ce64417d7868c4343f5d01d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Cell Proliferation</topic><topic>Down-Regulation</topic><topic>Hematology</topic><topic>HL-60 Cells - metabolism</topic><topic>Humans</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Oncology</topic><topic>Original Article</topic><topic>RNA - genetics</topic><topic>RNA - metabolism</topic><topic>RNA Interference</topic><topic>RNA, Small Interfering - genetics</topic><topic>RNA, Small Interfering - metabolism</topic><topic>Telomerase - genetics</topic><topic>Telomerase - metabolism</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miri-Moghaddam, E</creatorcontrib><creatorcontrib>Deezagi, A</creatorcontrib><creatorcontrib>Soheili, Z. S</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Annals of hematology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miri-Moghaddam, E</au><au>Deezagi, A</au><au>Soheili, Z. S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Downregulation of telomerase activity in human promyelocytic cell line using RNA interference</atitle><jtitle>Annals of hematology</jtitle><stitle>Ann Hematol</stitle><addtitle>Ann Hematol</addtitle><date>2009-12-01</date><risdate>2009</risdate><volume>88</volume><issue>12</issue><spage>1169</spage><epage>1176</epage><pages>1169-1176</pages><issn>0939-5555</issn><eissn>1432-0584</eissn><abstract>Telomerase is a ribonucleoprotein complex. It consists of two main components, human telomerase reverse transcriptase (hTERT) and human telomerase RNA. High telomerase activity is present in most malignant cells, but it is barely detectable in majority of somatic cells. The direct correlation between telomerase reactivation and carcinogens has made hTERT a key target for anticancer therapeutic studies. In this study, for the first time, we evaluated the ability of the new generation of short interfering RNA (siRNA) to regulate telomerase activity in the human promyelocytic leukemia cell line (HL-60). Transient transfection cell line by hTERT siRNAs resulted in statistically significant suppression of hTERT messenger RNAs which were detected by quantitative real-time polymerase chain reaction, while the expressed hTERT protein levels were measured by flow cytometry. The results of telomeric repeat amplification protocol showed that telomerase activity was significantly reduced upon transfection of the HL-60 cell line with hTERT siRNAs. The results of this study showed that telomerase activity and cell proliferation were efficiently inhibited in the hTERT siRNA-treated leukemic cell line.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>19415276</pmid><doi>10.1007/s00277-009-0748-0</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0939-5555 |
| ispartof | Annals of hematology, 2009-12, Vol.88 (12), p.1169-1176 |
| issn | 0939-5555 1432-0584 |
| language | eng |
| recordid | cdi_proquest_journals_219212183 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Cell Proliferation Down-Regulation Hematology HL-60 Cells - metabolism Humans Medicine Medicine & Public Health Oncology Original Article RNA - genetics RNA - metabolism RNA Interference RNA, Small Interfering - genetics RNA, Small Interfering - metabolism Telomerase - genetics Telomerase - metabolism Transfection |
| title | Downregulation of telomerase activity in human promyelocytic cell line using RNA interference |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T15%3A44%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Downregulation%20of%20telomerase%20activity%20in%20human%20promyelocytic%20cell%20line%20using%20RNA%20interference&rft.jtitle=Annals%20of%20hematology&rft.au=Miri-Moghaddam,%20E&rft.date=2009-12-01&rft.volume=88&rft.issue=12&rft.spage=1169&rft.epage=1176&rft.pages=1169-1176&rft.issn=0939-5555&rft.eissn=1432-0584&rft_id=info:doi/10.1007/s00277-009-0748-0&rft_dat=%3Cproquest_cross%3E1883464411%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=219212183&rft_id=info:pmid/19415276&rfr_iscdi=true |