Occurrence and seasonality of aflatoxin M1 in milk in two different climate zones
BACKGROUND Aflatoxin M1 (AFM1) is a mycotoxin found in milk as a result of the ingestion of aflatoxin B1 (AFB1) by dairy cattle. Because of its carcinogenic properties, the control of its occurrence in dairy products is of great importance. We evaluated the occurrence and seasonality of AFM1 in milk...
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| Veröffentlicht in: | Journal of the science of food and agriculture 2019-04, Vol.99 (6), p.3203-3206 |
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| container_title | Journal of the science of food and agriculture |
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| creator | Venâncio, Rafael Luiz Ludovico, Agostinho de Santana, Elsa Helena Walter de Toledo, Eduardo Amaral de Almeida Rego, Fabiola Cristine dos Santos, Joice Sifuentes |
| description | BACKGROUND
Aflatoxin M1 (AFM1) is a mycotoxin found in milk as a result of the ingestion of aflatoxin B1 (AFB1) by dairy cattle. Because of its carcinogenic properties, the control of its occurrence in dairy products is of great importance. We evaluated the occurrence and seasonality of AFM1 in milk from farms with subtropical and temperate climates, where significant milk production occurs. Forty samples of raw milk were collected from bulk tansk milk during the summer (five samples from a subtropical climate and 14 from a temperate climate) and winter (six samples from a subtropical climate and 15 from a temperate climate) months of 2017 and conducted an analysis using an enzyme‐linked immunosorbent assay (ELISA) (detection limit 5 ng L−1). Data from eight random samples were compared with respect to liquid chromatography with mass spectrometry detection and ELISA. A significant correlation was observed.
RESULTS
The presence of AFM1 was detected in 87.50% (n = 35) of the samples analyzed (a mean of 16.66 ng L−1 and a median of 12.42 ng L−1 in positive samples); however, its concentration in all samples was below the maximum limit allowed by European (50 ng L−1) and Brazilian (500 ng L−1) legislations. There were no significant differences in the levels of AFM1 between the properties located in the two climate zones, in both summer and winter. The estimated daily intake of AFM1 based on the milk analyzed was 0.0107 ng kg−1 day−1 for adolescents, 0.0072 ng kg−1 day−1 for adults and 0.0098 ng kg−1 day−1 for the elderly.
CONCLUSION
The present work demonstrated a low exposure to AFM1. © 2018 Society of Chemical Industry |
| doi_str_mv | 10.1002/jsfa.9487 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2191302394</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2191302394</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3537-5e7f71e6600df8a930ba77c9bbb623e8b992909a0e5175117f51b97d5b9dadef3</originalsourceid><addsrcrecordid>eNp1kD1PwzAQhi0EoqUw8AeQJSaGtOe4juuxqigfKqoQMFt2YkspaVzsRKX8ehxa2FjuHe65V6cHoUsCQwKQjlbBqqEYT_gR6hMQPAEgcIz6cZcmjIzTHjoLYQUAQmTZKepRGFNgIuuj52Wet96bOjdY1QUORgVXq6psdthZrGylGvdZ1viJ4DjXZfXeZbN1uCitNfGywXlVrlVj8JerTThHJ1ZVwVwccoDe5revs_tksbx7mE0XSU4Z5Qkz3HJisgygsBMlKGjFeS601llKzUQLkQoQCgwjnBHCLSNa8IJpUajCWDpA1_vejXcfrQmNXLnWx9eDTIkgFFIqxpG62VO5dyF4Y-XGx2f9ThKQnTzZyZOdvMheHRpbvTbFH_lrKwKjPbAtK7P7v0k-vsynP5XfTl94zg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2191302394</pqid></control><display><type>article</type><title>Occurrence and seasonality of aflatoxin M1 in milk in two different climate zones</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Venâncio, Rafael Luiz ; Ludovico, Agostinho ; de Santana, Elsa Helena Walter ; de Toledo, Eduardo Amaral ; de Almeida Rego, Fabiola Cristine ; dos Santos, Joice Sifuentes</creator><creatorcontrib>Venâncio, Rafael Luiz ; Ludovico, Agostinho ; de Santana, Elsa Helena Walter ; de Toledo, Eduardo Amaral ; de Almeida Rego, Fabiola Cristine ; dos Santos, Joice Sifuentes</creatorcontrib><description>BACKGROUND
Aflatoxin M1 (AFM1) is a mycotoxin found in milk as a result of the ingestion of aflatoxin B1 (AFB1) by dairy cattle. Because of its carcinogenic properties, the control of its occurrence in dairy products is of great importance. We evaluated the occurrence and seasonality of AFM1 in milk from farms with subtropical and temperate climates, where significant milk production occurs. Forty samples of raw milk were collected from bulk tansk milk during the summer (five samples from a subtropical climate and 14 from a temperate climate) and winter (six samples from a subtropical climate and 15 from a temperate climate) months of 2017 and conducted an analysis using an enzyme‐linked immunosorbent assay (ELISA) (detection limit 5 ng L−1). Data from eight random samples were compared with respect to liquid chromatography with mass spectrometry detection and ELISA. A significant correlation was observed.
RESULTS
The presence of AFM1 was detected in 87.50% (n = 35) of the samples analyzed (a mean of 16.66 ng L−1 and a median of 12.42 ng L−1 in positive samples); however, its concentration in all samples was below the maximum limit allowed by European (50 ng L−1) and Brazilian (500 ng L−1) legislations. There were no significant differences in the levels of AFM1 between the properties located in the two climate zones, in both summer and winter. The estimated daily intake of AFM1 based on the milk analyzed was 0.0107 ng kg−1 day−1 for adolescents, 0.0072 ng kg−1 day−1 for adults and 0.0098 ng kg−1 day−1 for the elderly.
CONCLUSION
The present work demonstrated a low exposure to AFM1. © 2018 Society of Chemical Industry</description><identifier>ISSN: 0022-5142</identifier><identifier>EISSN: 1097-0010</identifier><identifier>DOI: 10.1002/jsfa.9487</identifier><identifier>PMID: 30430596</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Adolescents ; Adults ; Aflatoxin B1 ; Aflatoxin M1 ; Aflatoxin M1 - analysis ; Aflatoxin M1 - metabolism ; Animals ; Brazil ; Carcinogens ; Cattle ; Chromatography, Liquid ; Climate ; Consumer Product Safety ; Dairy products ; Enzyme-Linked Immunosorbent Assay ; estimated daily intake ; Farms ; Food Contamination - analysis ; Geriatrics ; Humans ; immunoassay ; Ingestion ; Liquid chromatography ; Mass spectrometry ; Mass spectroscopy ; Milk ; Milk - chemistry ; Milk - metabolism ; Milk production ; Older people ; Organic chemistry ; raw milk ; Seasonal variations ; Seasons ; Summer ; survey ; Winter</subject><ispartof>Journal of the science of food and agriculture, 2019-04, Vol.99 (6), p.3203-3206</ispartof><rights>2018 Society of Chemical Industry</rights><rights>2018 Society of Chemical Industry.</rights><rights>2019 Society of Chemical Industry</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3537-5e7f71e6600df8a930ba77c9bbb623e8b992909a0e5175117f51b97d5b9dadef3</citedby><cites>FETCH-LOGICAL-c3537-5e7f71e6600df8a930ba77c9bbb623e8b992909a0e5175117f51b97d5b9dadef3</cites><orcidid>0000-0002-1089-0620</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjsfa.9487$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjsfa.9487$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30430596$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Venâncio, Rafael Luiz</creatorcontrib><creatorcontrib>Ludovico, Agostinho</creatorcontrib><creatorcontrib>de Santana, Elsa Helena Walter</creatorcontrib><creatorcontrib>de Toledo, Eduardo Amaral</creatorcontrib><creatorcontrib>de Almeida Rego, Fabiola Cristine</creatorcontrib><creatorcontrib>dos Santos, Joice Sifuentes</creatorcontrib><title>Occurrence and seasonality of aflatoxin M1 in milk in two different climate zones</title><title>Journal of the science of food and agriculture</title><addtitle>J Sci Food Agric</addtitle><description>BACKGROUND
Aflatoxin M1 (AFM1) is a mycotoxin found in milk as a result of the ingestion of aflatoxin B1 (AFB1) by dairy cattle. Because of its carcinogenic properties, the control of its occurrence in dairy products is of great importance. We evaluated the occurrence and seasonality of AFM1 in milk from farms with subtropical and temperate climates, where significant milk production occurs. Forty samples of raw milk were collected from bulk tansk milk during the summer (five samples from a subtropical climate and 14 from a temperate climate) and winter (six samples from a subtropical climate and 15 from a temperate climate) months of 2017 and conducted an analysis using an enzyme‐linked immunosorbent assay (ELISA) (detection limit 5 ng L−1). Data from eight random samples were compared with respect to liquid chromatography with mass spectrometry detection and ELISA. A significant correlation was observed.
RESULTS
The presence of AFM1 was detected in 87.50% (n = 35) of the samples analyzed (a mean of 16.66 ng L−1 and a median of 12.42 ng L−1 in positive samples); however, its concentration in all samples was below the maximum limit allowed by European (50 ng L−1) and Brazilian (500 ng L−1) legislations. There were no significant differences in the levels of AFM1 between the properties located in the two climate zones, in both summer and winter. The estimated daily intake of AFM1 based on the milk analyzed was 0.0107 ng kg−1 day−1 for adolescents, 0.0072 ng kg−1 day−1 for adults and 0.0098 ng kg−1 day−1 for the elderly.
CONCLUSION
The present work demonstrated a low exposure to AFM1. © 2018 Society of Chemical Industry</description><subject>Adolescents</subject><subject>Adults</subject><subject>Aflatoxin B1</subject><subject>Aflatoxin M1</subject><subject>Aflatoxin M1 - analysis</subject><subject>Aflatoxin M1 - metabolism</subject><subject>Animals</subject><subject>Brazil</subject><subject>Carcinogens</subject><subject>Cattle</subject><subject>Chromatography, Liquid</subject><subject>Climate</subject><subject>Consumer Product Safety</subject><subject>Dairy products</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>estimated daily intake</subject><subject>Farms</subject><subject>Food Contamination - analysis</subject><subject>Geriatrics</subject><subject>Humans</subject><subject>immunoassay</subject><subject>Ingestion</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Milk</subject><subject>Milk - chemistry</subject><subject>Milk - metabolism</subject><subject>Milk production</subject><subject>Older people</subject><subject>Organic chemistry</subject><subject>raw milk</subject><subject>Seasonal variations</subject><subject>Seasons</subject><subject>Summer</subject><subject>survey</subject><subject>Winter</subject><issn>0022-5142</issn><issn>1097-0010</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kD1PwzAQhi0EoqUw8AeQJSaGtOe4juuxqigfKqoQMFt2YkspaVzsRKX8ehxa2FjuHe65V6cHoUsCQwKQjlbBqqEYT_gR6hMQPAEgcIz6cZcmjIzTHjoLYQUAQmTZKepRGFNgIuuj52Wet96bOjdY1QUORgVXq6psdthZrGylGvdZ1viJ4DjXZfXeZbN1uCitNfGywXlVrlVj8JerTThHJ1ZVwVwccoDe5revs_tksbx7mE0XSU4Z5Qkz3HJisgygsBMlKGjFeS601llKzUQLkQoQCgwjnBHCLSNa8IJpUajCWDpA1_vejXcfrQmNXLnWx9eDTIkgFFIqxpG62VO5dyF4Y-XGx2f9ThKQnTzZyZOdvMheHRpbvTbFH_lrKwKjPbAtK7P7v0k-vsynP5XfTl94zg</recordid><startdate>201904</startdate><enddate>201904</enddate><creator>Venâncio, Rafael Luiz</creator><creator>Ludovico, Agostinho</creator><creator>de Santana, Elsa Helena Walter</creator><creator>de Toledo, Eduardo Amaral</creator><creator>de Almeida Rego, Fabiola Cristine</creator><creator>dos Santos, Joice Sifuentes</creator><general>John Wiley & Sons, Ltd</general><general>John Wiley and Sons, Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QL</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SN</scope><scope>7SP</scope><scope>7SR</scope><scope>7ST</scope><scope>7T5</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope><orcidid>https://orcid.org/0000-0002-1089-0620</orcidid></search><sort><creationdate>201904</creationdate><title>Occurrence and seasonality of aflatoxin M1 in milk in two different climate zones</title><author>Venâncio, Rafael Luiz ; 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Aflatoxin M1 (AFM1) is a mycotoxin found in milk as a result of the ingestion of aflatoxin B1 (AFB1) by dairy cattle. Because of its carcinogenic properties, the control of its occurrence in dairy products is of great importance. We evaluated the occurrence and seasonality of AFM1 in milk from farms with subtropical and temperate climates, where significant milk production occurs. Forty samples of raw milk were collected from bulk tansk milk during the summer (five samples from a subtropical climate and 14 from a temperate climate) and winter (six samples from a subtropical climate and 15 from a temperate climate) months of 2017 and conducted an analysis using an enzyme‐linked immunosorbent assay (ELISA) (detection limit 5 ng L−1). Data from eight random samples were compared with respect to liquid chromatography with mass spectrometry detection and ELISA. A significant correlation was observed.
RESULTS
The presence of AFM1 was detected in 87.50% (n = 35) of the samples analyzed (a mean of 16.66 ng L−1 and a median of 12.42 ng L−1 in positive samples); however, its concentration in all samples was below the maximum limit allowed by European (50 ng L−1) and Brazilian (500 ng L−1) legislations. There were no significant differences in the levels of AFM1 between the properties located in the two climate zones, in both summer and winter. The estimated daily intake of AFM1 based on the milk analyzed was 0.0107 ng kg−1 day−1 for adolescents, 0.0072 ng kg−1 day−1 for adults and 0.0098 ng kg−1 day−1 for the elderly.
CONCLUSION
The present work demonstrated a low exposure to AFM1. © 2018 Society of Chemical Industry</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>30430596</pmid><doi>10.1002/jsfa.9487</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0002-1089-0620</orcidid></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Adolescents Adults Aflatoxin B1 Aflatoxin M1 Aflatoxin M1 - analysis Aflatoxin M1 - metabolism Animals Brazil Carcinogens Cattle Chromatography, Liquid Climate Consumer Product Safety Dairy products Enzyme-Linked Immunosorbent Assay estimated daily intake Farms Food Contamination - analysis Geriatrics Humans immunoassay Ingestion Liquid chromatography Mass spectrometry Mass spectroscopy Milk Milk - chemistry Milk - metabolism Milk production Older people Organic chemistry raw milk Seasonal variations Seasons Summer survey Winter |
| title | Occurrence and seasonality of aflatoxin M1 in milk in two different climate zones |
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