Differentiation of Aneugens and Clastogens in the In Vitro Micronucleus Test by Kinetochore Scoring Using Automated Image Analysis

The in vitro micronucleus test according to OECD Test Guideline 487 (TG 487) is widely used to investigate the genotoxic potential of drugs. Besides the identification of in vitro genotoxicants, the assay can be complemented with kinetochore staining for the differentiation between clastogens and an...

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Veröffentlicht in:	Environmental and molecular mutagenesis 2019-04, Vol.60 (3), p.227-242
Hauptverfasser:	Wilde, Sabrina, Queisser, Nina, Holz, Christian, Raschke, Marian, Sutter, Andreas
Format:	Artikel
Sprache:	eng
Schlagworte:	aneugen Aneugens - pharmacology Animals Antibodies Automation Cell Line clastogen Cricetinae Cytochalasin B Cytokinesis cytokinesis‐block micronucleus test Differentiation DNA Damage - drug effects Dose-Response Relationship, Drug Genotoxicity Human bias Image analysis Image processing Image Processing, Computer-Assisted - methods In vitro methods and tests kinetochore staining Kinetochores Kinetochores - drug effects Micronuclei, Chromosome-Defective - chemically induced Micronucleus Tests - methods Microscopic analysis Mutagens - pharmacology Nuclei (cytology) Potassium Risk assessment Sensitivity Sensitivity analysis Staining and Labeling - methods
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