Disruption of the AtREV3 gene causes hypersensitivity to ultraviolet B light and (gamma-rays) in Arabidopsis: Implication of the presence of a translesion synthesis mechanism in plants
To investigate UV light response mechanisms in higher plants, we isolated a UV light-sensitive mutant, rev3-1, in Arabidopsis. The root growth of rev3-1 was inhibited after UV-B irradiation under both light and dark conditions. We found that chromosome 1 of rev3-1 was broken at a minimum of three po...
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| Veröffentlicht in: | The Plant cell 2003-09, Vol.15 (9), p.2042 |
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| creator | Sakamoto, Ayako Vo Thi Thuong Lan Hase, Yoshihiro Shikazono, Naoya |
| description | To investigate UV light response mechanisms in higher plants, we isolated a UV light-sensitive mutant, rev3-1, in Arabidopsis. The root growth of rev3-1 was inhibited after UV-B irradiation under both light and dark conditions. We found that chromosome 1 of rev3-1 was broken at a minimum of three points, causing chromosome inversion and translocation. A gene disrupted by this rearrangement encoded the catalytic subunit of DNA polymerase zeta (AtREV3), which is thought to be involved in translesion synthesis. The rev3-1 seedlings also were sensitive to gamma-rays and mitomycin C, which are known to inhibit DNA replication. Incorporation of bromodeoxyuridine after UV-B irradiation was less in rev3-1 than in the wild type. These results indicate that UV light-damaged DNA interrupted DNA replication in the rev3-1 mutant, leading to the inhibition of cell division and root elongation. |
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The root growth of rev3-1 was inhibited after UV-B irradiation under both light and dark conditions. We found that chromosome 1 of rev3-1 was broken at a minimum of three points, causing chromosome inversion and translocation. A gene disrupted by this rearrangement encoded the catalytic subunit of DNA polymerase zeta (AtREV3), which is thought to be involved in translesion synthesis. The rev3-1 seedlings also were sensitive to gamma-rays and mitomycin C, which are known to inhibit DNA replication. Incorporation of bromodeoxyuridine after UV-B irradiation was less in rev3-1 than in the wild type. 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These results indicate that UV light-damaged DNA interrupted DNA replication in the rev3-1 mutant, leading to the inhibition of cell division and root elongation.</description><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Gamma rays</subject><subject>Hypersensitivity</subject><subject>Irradiation</subject><subject>Plant growth</subject><subject>Seedlings</subject><subject>Translocation</subject><subject>Ultraviolet radiation</subject><issn>1040-4651</issn><issn>1532-298X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNqNjcFKw0AQhoMoWKvvMHjSQ2CTtE3qrWqLXqWItzKm02TKZnfd2RTyZj6eG_Dg0dPMz3z_N2fJJJsXeZovq4_zuKuZSmeLeXaZXIkclVJZmS0nyfczi-9dYGvAHiC0BKvwtn4voCFDUGMvJNAOjryQEQ584jBAsNDr4PHEVlOAR9DctAHQ7OGuwa7D1OMg98AGVh4_eW-dsDzAa-c01_j3nfMUzTWNGSE6jWiSEZDBRCD2oKO6RcPSjUKn0QS5Ti4OqIVufuc0ud2st08vqfP2qycJu6PtvYmnXZ5V5aJSZVX8C_oBaeVmqQ</recordid><startdate>20030901</startdate><enddate>20030901</enddate><creator>Sakamoto, Ayako</creator><creator>Vo Thi Thuong Lan</creator><creator>Hase, Yoshihiro</creator><creator>Shikazono, Naoya</creator><general>American Society of Plant Biologists</general><scope>3V.</scope><scope>4T-</scope><scope>7QO</scope><scope>7TM</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>S0X</scope></search><sort><creationdate>20030901</creationdate><title>Disruption of the AtREV3 gene causes hypersensitivity to ultraviolet B light and (gamma-rays) in Arabidopsis: Implication of the presence of a translesion synthesis mechanism in plants</title><author>Sakamoto, Ayako ; 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| ispartof | The Plant cell, 2003-09, Vol.15 (9), p.2042 |
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| source | Jstor Complete Legacy; Oxford University Press Journals All Titles (1996-Current); Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Deoxyribonucleic acid DNA Gamma rays Hypersensitivity Irradiation Plant growth Seedlings Translocation Ultraviolet radiation |
| title | Disruption of the AtREV3 gene causes hypersensitivity to ultraviolet B light and (gamma-rays) in Arabidopsis: Implication of the presence of a translesion synthesis mechanism in plants |
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