Preparation of copolymer-grafted mixed-mode resins for immunoglobulin G adsorption
The mixed-mode resins for protein adsorption have been prepared by a novel strategy, copolymer grafting. Specially, the copolymer-grafted resins CG-M-A with two functional groups, 5-amino-benzimidazole (ABI) and methacryloxyethyltrimethyl ammonium chloride (METAC), have been prepared through surface...
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| Veröffentlicht in: | Frontiers of chemical science and engineering 2019-03, Vol.13 (1), p.70-79 |
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| creator | Chen, Shenggang Liu, Tao Yang, Ruiqi Lin, Dongqiang Yao, Shanjing |
| description | The mixed-mode resins for protein adsorption have been prepared by a novel strategy, copolymer grafting. Specially, the copolymer-grafted resins CG-M-A with two functional groups, 5-amino-benzimidazole (ABI) and methacryloxyethyltrimethyl ammonium chloride (METAC), have been prepared through surface-initiated activator generated by electron transfer for atom transfer radical polymerization of METAC and glycidyl methacrylate (GMA), followed by a ring-open reaction to introduce ABI. The charge and hydrophobicity of CG-MA resins could be controlled by manipulating the addition of METAC and GMA/ABI. Besides, METAC and ABI provided positive effects together in both protein adsorption and elution: dynamic binding capacity of human Immunoglobulin G (hIgG) onto CG-M-A resin with the highest ligand ratio of METAC to ABI is 46.8 mg∙g
–1
at pH 9 and the elution recovery of hIgG is 97.0% at pH 5. The separation experiment showed that purity and recovery of monoclonal antibody from cell culture supernatant are 96.0% and 86.5%, respectively, indicating that copolymer-grafted mixed-mode resins could be used for antibody purification. |
| doi_str_mv | 10.1007/s11705-018-1745-4 |
| format | Article |
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–1
at pH 9 and the elution recovery of hIgG is 97.0% at pH 5. The separation experiment showed that purity and recovery of monoclonal antibody from cell culture supernatant are 96.0% and 86.5%, respectively, indicating that copolymer-grafted mixed-mode resins could be used for antibody purification.</description><identifier>ISSN: 2095-0179</identifier><identifier>EISSN: 2095-0187</identifier><identifier>DOI: 10.1007/s11705-018-1745-4</identifier><language>eng</language><publisher>Beijing: Higher Education Press</publisher><subject>Adsorption ; Ammonium chloride ; Chemistry ; Chemistry and Materials Science ; Copolymers ; Electron transfer ; Elution ; Functional groups ; Graft copolymers ; Hydrophobicity ; Immunoglobulins ; Industrial Chemistry/Chemical Engineering ; Monoclonal antibodies ; Nanotechnology ; Polymers ; Protein adsorption ; Proteins ; Recovery ; Research Article ; Resins</subject><ispartof>Frontiers of chemical science and engineering, 2019-03, Vol.13 (1), p.70-79</ispartof><rights>Higher Education Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018</rights><rights>Copyright Springer Nature B.V. 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-32a9c89e4bb6af4619f8d4fadcc7e0d5dc406ac2cb4c4d87bb32c60b100897473</citedby><cites>FETCH-LOGICAL-c365t-32a9c89e4bb6af4619f8d4fadcc7e0d5dc406ac2cb4c4d87bb32c60b100897473</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11705-018-1745-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11705-018-1745-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Chen, Shenggang</creatorcontrib><creatorcontrib>Liu, Tao</creatorcontrib><creatorcontrib>Yang, Ruiqi</creatorcontrib><creatorcontrib>Lin, Dongqiang</creatorcontrib><creatorcontrib>Yao, Shanjing</creatorcontrib><title>Preparation of copolymer-grafted mixed-mode resins for immunoglobulin G adsorption</title><title>Frontiers of chemical science and engineering</title><addtitle>Front. Chem. Sci. Eng</addtitle><description>The mixed-mode resins for protein adsorption have been prepared by a novel strategy, copolymer grafting. Specially, the copolymer-grafted resins CG-M-A with two functional groups, 5-amino-benzimidazole (ABI) and methacryloxyethyltrimethyl ammonium chloride (METAC), have been prepared through surface-initiated activator generated by electron transfer for atom transfer radical polymerization of METAC and glycidyl methacrylate (GMA), followed by a ring-open reaction to introduce ABI. The charge and hydrophobicity of CG-MA resins could be controlled by manipulating the addition of METAC and GMA/ABI. Besides, METAC and ABI provided positive effects together in both protein adsorption and elution: dynamic binding capacity of human Immunoglobulin G (hIgG) onto CG-M-A resin with the highest ligand ratio of METAC to ABI is 46.8 mg∙g
–1
at pH 9 and the elution recovery of hIgG is 97.0% at pH 5. The separation experiment showed that purity and recovery of monoclonal antibody from cell culture supernatant are 96.0% and 86.5%, respectively, indicating that copolymer-grafted mixed-mode resins could be used for antibody purification.</description><subject>Adsorption</subject><subject>Ammonium chloride</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Copolymers</subject><subject>Electron transfer</subject><subject>Elution</subject><subject>Functional groups</subject><subject>Graft copolymers</subject><subject>Hydrophobicity</subject><subject>Immunoglobulins</subject><subject>Industrial Chemistry/Chemical Engineering</subject><subject>Monoclonal antibodies</subject><subject>Nanotechnology</subject><subject>Polymers</subject><subject>Protein adsorption</subject><subject>Proteins</subject><subject>Recovery</subject><subject>Research Article</subject><subject>Resins</subject><issn>2095-0179</issn><issn>2095-0187</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kEtLxDAUhYMoOIzzA9wFXEfzatIuZdBRGFBE1yHPUmmbmrTg_Hs7VnTl6l4u55zL-QC4JPiaYCxvMiESFwiTEhHJC8RPwIri6vsiT393WZ2DTc6NwYxQwZiUK_DynPygkx6b2MMYoI1DbA-dT6hOOozewa759A510XmYfG76DENMsOm6qY91G83UNj3cQe1yTMMx5gKcBd1mv_mZa_B2f_e6fUD7p93j9naPLBPFiBjVlS0rz40ROnBBqlA6HrSzVnrsCmc5FtpSa7jlrpTGMGoFNnPjspJcsjW4WnKHFD8mn0f1HqfUzy8VJaWQjBQSzyqyqGyKOScf1JCaTqeDIlgd6amFnppZqSM9xWcPXTx51va1T3_J_5u-AJqfc2Q</recordid><startdate>20190301</startdate><enddate>20190301</enddate><creator>Chen, Shenggang</creator><creator>Liu, Tao</creator><creator>Yang, Ruiqi</creator><creator>Lin, Dongqiang</creator><creator>Yao, Shanjing</creator><general>Higher Education Press</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20190301</creationdate><title>Preparation of copolymer-grafted mixed-mode resins for immunoglobulin G adsorption</title><author>Chen, Shenggang ; Liu, Tao ; Yang, Ruiqi ; Lin, Dongqiang ; Yao, Shanjing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-32a9c89e4bb6af4619f8d4fadcc7e0d5dc406ac2cb4c4d87bb32c60b100897473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adsorption</topic><topic>Ammonium chloride</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Copolymers</topic><topic>Electron transfer</topic><topic>Elution</topic><topic>Functional groups</topic><topic>Graft copolymers</topic><topic>Hydrophobicity</topic><topic>Immunoglobulins</topic><topic>Industrial Chemistry/Chemical Engineering</topic><topic>Monoclonal antibodies</topic><topic>Nanotechnology</topic><topic>Polymers</topic><topic>Protein adsorption</topic><topic>Proteins</topic><topic>Recovery</topic><topic>Research Article</topic><topic>Resins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Shenggang</creatorcontrib><creatorcontrib>Liu, Tao</creatorcontrib><creatorcontrib>Yang, Ruiqi</creatorcontrib><creatorcontrib>Lin, Dongqiang</creatorcontrib><creatorcontrib>Yao, Shanjing</creatorcontrib><collection>CrossRef</collection><jtitle>Frontiers of chemical science and engineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Shenggang</au><au>Liu, Tao</au><au>Yang, Ruiqi</au><au>Lin, Dongqiang</au><au>Yao, Shanjing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation of copolymer-grafted mixed-mode resins for immunoglobulin G adsorption</atitle><jtitle>Frontiers of chemical science and engineering</jtitle><stitle>Front. Chem. Sci. Eng</stitle><date>2019-03-01</date><risdate>2019</risdate><volume>13</volume><issue>1</issue><spage>70</spage><epage>79</epage><pages>70-79</pages><issn>2095-0179</issn><eissn>2095-0187</eissn><abstract>The mixed-mode resins for protein adsorption have been prepared by a novel strategy, copolymer grafting. Specially, the copolymer-grafted resins CG-M-A with two functional groups, 5-amino-benzimidazole (ABI) and methacryloxyethyltrimethyl ammonium chloride (METAC), have been prepared through surface-initiated activator generated by electron transfer for atom transfer radical polymerization of METAC and glycidyl methacrylate (GMA), followed by a ring-open reaction to introduce ABI. The charge and hydrophobicity of CG-MA resins could be controlled by manipulating the addition of METAC and GMA/ABI. Besides, METAC and ABI provided positive effects together in both protein adsorption and elution: dynamic binding capacity of human Immunoglobulin G (hIgG) onto CG-M-A resin with the highest ligand ratio of METAC to ABI is 46.8 mg∙g
–1
at pH 9 and the elution recovery of hIgG is 97.0% at pH 5. The separation experiment showed that purity and recovery of monoclonal antibody from cell culture supernatant are 96.0% and 86.5%, respectively, indicating that copolymer-grafted mixed-mode resins could be used for antibody purification.</abstract><cop>Beijing</cop><pub>Higher Education Press</pub><doi>10.1007/s11705-018-1745-4</doi><tpages>10</tpages></addata></record> |
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| subjects | Adsorption Ammonium chloride Chemistry Chemistry and Materials Science Copolymers Electron transfer Elution Functional groups Graft copolymers Hydrophobicity Immunoglobulins Industrial Chemistry/Chemical Engineering Monoclonal antibodies Nanotechnology Polymers Protein adsorption Proteins Recovery Research Article Resins |
| title | Preparation of copolymer-grafted mixed-mode resins for immunoglobulin G adsorption |
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