BSA-seq mapping reveals major QTL for broomrape resistance in four sunflower lines
Broomrape ( Orobanche cumana ) is a parasitic weed that causes substantial yield losses in sunflower. In this study, four biparental genetic populations comprised of between 96 and 150 F 3 families were phenotyped for resistance to broomrape race G. Bulk segregant analyses (BSA) combined with genoty...
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| Veröffentlicht in: | Molecular breeding 2019-03, Vol.39 (3), p.1-15, Article 41 |
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| container_title | Molecular breeding |
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| creator | Imerovski, Ivana Dedić, Boško Cvejić, Sandra Miladinović, Dragana Jocić, Siniša Owens, Gregory L. Tubić, Nataša Kočiš Rieseberg, Loren H. |
| description | Broomrape (
Orobanche cumana
) is a parasitic weed that causes substantial yield losses in sunflower. In this study, four biparental genetic populations comprised of between 96 and 150 F
3
families were phenotyped for resistance to broomrape race G. Bulk segregant analyses (BSA) combined with genotyping-by-sequencing (GBS) technology was used to verify previously identified genes and map new resistance QTLs. Resistance had a polygenic basis, and numerous QTLs were found in all mapping populations. Contributing components to resistance that were common to all populations mapped to two major QTLs on chromosome 3, which were designated
or3.
1 and
or3.
2. QTL
or3.
1 was positioned in a genomic region where a previous broomrape resistance gene
Or5
has been mapped, while QTL
or3.
2 was identified for the first time in the lower region of the same chromosome. Following the identification of major QTLs for resistance using the BSA-seq approach, all plants from three F
2
populations were genotyped using newly developed CAPS markers nearest to the QTL peak for
or3.
2, which confirmed the association of these markers with broomrape race G resistance. The results of this study will bring us a step closer to understanding the mechanisms underlying resistance of sunflower to highly virulent broomrape races, and the molecular markers developed herein will be highly useful for sunflower breeding programs. |
| doi_str_mv | 10.1007/s11032-019-0948-9 |
| format | Article |
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Orobanche cumana
) is a parasitic weed that causes substantial yield losses in sunflower. In this study, four biparental genetic populations comprised of between 96 and 150 F
3
families were phenotyped for resistance to broomrape race G. Bulk segregant analyses (BSA) combined with genotyping-by-sequencing (GBS) technology was used to verify previously identified genes and map new resistance QTLs. Resistance had a polygenic basis, and numerous QTLs were found in all mapping populations. Contributing components to resistance that were common to all populations mapped to two major QTLs on chromosome 3, which were designated
or3.
1 and
or3.
2. QTL
or3.
1 was positioned in a genomic region where a previous broomrape resistance gene
Or5
has been mapped, while QTL
or3.
2 was identified for the first time in the lower region of the same chromosome. Following the identification of major QTLs for resistance using the BSA-seq approach, all plants from three F
2
populations were genotyped using newly developed CAPS markers nearest to the QTL peak for
or3.
2, which confirmed the association of these markers with broomrape race G resistance. The results of this study will bring us a step closer to understanding the mechanisms underlying resistance of sunflower to highly virulent broomrape races, and the molecular markers developed herein will be highly useful for sunflower breeding programs.</description><identifier>ISSN: 1380-3743</identifier><identifier>EISSN: 1572-9788</identifier><identifier>DOI: 10.1007/s11032-019-0948-9</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Biomedical and Life Sciences ; Biotechnology ; Chromosome 3 ; Gene mapping ; Gene sequencing ; Genotyping ; Helianthus ; Life Sciences ; Mapping ; Markers ; Molecular biology ; Plant biology ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Population genetics ; Population studies ; Populations ; Quantitative trait loci</subject><ispartof>Molecular breeding, 2019-03, Vol.39 (3), p.1-15, Article 41</ispartof><rights>Springer Nature B.V. 2019</rights><rights>Copyright Springer Nature B.V. 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-d11e1f5bc465bafb56ba25d6a6f646f40ff6acfabb77a4cd181204f0cdf50e0f3</citedby><cites>FETCH-LOGICAL-c316t-d11e1f5bc465bafb56ba25d6a6f646f40ff6acfabb77a4cd181204f0cdf50e0f3</cites><orcidid>0000-0002-1164-3664</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11032-019-0948-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11032-019-0948-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Imerovski, Ivana</creatorcontrib><creatorcontrib>Dedić, Boško</creatorcontrib><creatorcontrib>Cvejić, Sandra</creatorcontrib><creatorcontrib>Miladinović, Dragana</creatorcontrib><creatorcontrib>Jocić, Siniša</creatorcontrib><creatorcontrib>Owens, Gregory L.</creatorcontrib><creatorcontrib>Tubić, Nataša Kočiš</creatorcontrib><creatorcontrib>Rieseberg, Loren H.</creatorcontrib><title>BSA-seq mapping reveals major QTL for broomrape resistance in four sunflower lines</title><title>Molecular breeding</title><addtitle>Mol Breeding</addtitle><description>Broomrape (
Orobanche cumana
) is a parasitic weed that causes substantial yield losses in sunflower. In this study, four biparental genetic populations comprised of between 96 and 150 F
3
families were phenotyped for resistance to broomrape race G. Bulk segregant analyses (BSA) combined with genotyping-by-sequencing (GBS) technology was used to verify previously identified genes and map new resistance QTLs. Resistance had a polygenic basis, and numerous QTLs were found in all mapping populations. Contributing components to resistance that were common to all populations mapped to two major QTLs on chromosome 3, which were designated
or3.
1 and
or3.
2. QTL
or3.
1 was positioned in a genomic region where a previous broomrape resistance gene
Or5
has been mapped, while QTL
or3.
2 was identified for the first time in the lower region of the same chromosome. Following the identification of major QTLs for resistance using the BSA-seq approach, all plants from three F
2
populations were genotyped using newly developed CAPS markers nearest to the QTL peak for
or3.
2, which confirmed the association of these markers with broomrape race G resistance. The results of this study will bring us a step closer to understanding the mechanisms underlying resistance of sunflower to highly virulent broomrape races, and the molecular markers developed herein will be highly useful for sunflower breeding programs.</description><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Chromosome 3</subject><subject>Gene mapping</subject><subject>Gene sequencing</subject><subject>Genotyping</subject><subject>Helianthus</subject><subject>Life Sciences</subject><subject>Mapping</subject><subject>Markers</subject><subject>Molecular biology</subject><subject>Plant biology</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Population genetics</subject><subject>Population studies</subject><subject>Populations</subject><subject>Quantitative trait loci</subject><issn>1380-3743</issn><issn>1572-9788</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1UE1LAzEQDaJgrf4AbwueozO7STY51uIXFESt55DNJmVLm90mreK_N7KCJ09vhvcxwyPkEuEaAeqbhAhVSQEVBcUkVUdkgrwuqaqlPM5zJYFWNatOyVlKa8geJcSEvN6-zWhyu2JrhqELqyK6D2c2Ke_rPhYvy0XhMzax77fRDC7zqUt7E6wrupC5QyzSIfhN_-lisemCS-fkxOcEd_GLU_J-f7ecP9LF88PTfLagtkKxpy2iQ88bywRvjG-4aEzJW2GEF0x4Bt4LY71pmro2zLYosQTmwbaegwNfTcnVmDvEfndwaa_X-ZuQT-oSpRCKg2RZhaPKxj6l6LweYrc18Usj6J_q9FidztXpn-q0yp5y9KSsDSsX_5L_N30DkxBySQ</recordid><startdate>20190301</startdate><enddate>20190301</enddate><creator>Imerovski, Ivana</creator><creator>Dedić, Boško</creator><creator>Cvejić, Sandra</creator><creator>Miladinović, Dragana</creator><creator>Jocić, Siniša</creator><creator>Owens, Gregory L.</creator><creator>Tubić, Nataša Kočiš</creator><creator>Rieseberg, Loren H.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0002-1164-3664</orcidid></search><sort><creationdate>20190301</creationdate><title>BSA-seq mapping reveals major QTL for broomrape resistance in four sunflower lines</title><author>Imerovski, Ivana ; Dedić, Boško ; Cvejić, Sandra ; Miladinović, Dragana ; Jocić, Siniša ; Owens, Gregory L. ; Tubić, Nataša Kočiš ; Rieseberg, Loren H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-d11e1f5bc465bafb56ba25d6a6f646f40ff6acfabb77a4cd181204f0cdf50e0f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Chromosome 3</topic><topic>Gene mapping</topic><topic>Gene sequencing</topic><topic>Genotyping</topic><topic>Helianthus</topic><topic>Life Sciences</topic><topic>Mapping</topic><topic>Markers</topic><topic>Molecular biology</topic><topic>Plant biology</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Population genetics</topic><topic>Population studies</topic><topic>Populations</topic><topic>Quantitative trait loci</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Imerovski, Ivana</creatorcontrib><creatorcontrib>Dedić, Boško</creatorcontrib><creatorcontrib>Cvejić, Sandra</creatorcontrib><creatorcontrib>Miladinović, Dragana</creatorcontrib><creatorcontrib>Jocić, Siniša</creatorcontrib><creatorcontrib>Owens, Gregory L.</creatorcontrib><creatorcontrib>Tubić, Nataša Kočiš</creatorcontrib><creatorcontrib>Rieseberg, Loren H.</creatorcontrib><collection>CrossRef</collection><jtitle>Molecular breeding</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Imerovski, Ivana</au><au>Dedić, Boško</au><au>Cvejić, Sandra</au><au>Miladinović, Dragana</au><au>Jocić, Siniša</au><au>Owens, Gregory L.</au><au>Tubić, Nataša Kočiš</au><au>Rieseberg, Loren H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>BSA-seq mapping reveals major QTL for broomrape resistance in four sunflower lines</atitle><jtitle>Molecular breeding</jtitle><stitle>Mol Breeding</stitle><date>2019-03-01</date><risdate>2019</risdate><volume>39</volume><issue>3</issue><spage>1</spage><epage>15</epage><pages>1-15</pages><artnum>41</artnum><issn>1380-3743</issn><eissn>1572-9788</eissn><abstract>Broomrape (
Orobanche cumana
) is a parasitic weed that causes substantial yield losses in sunflower. In this study, four biparental genetic populations comprised of between 96 and 150 F
3
families were phenotyped for resistance to broomrape race G. Bulk segregant analyses (BSA) combined with genotyping-by-sequencing (GBS) technology was used to verify previously identified genes and map new resistance QTLs. Resistance had a polygenic basis, and numerous QTLs were found in all mapping populations. Contributing components to resistance that were common to all populations mapped to two major QTLs on chromosome 3, which were designated
or3.
1 and
or3.
2. QTL
or3.
1 was positioned in a genomic region where a previous broomrape resistance gene
Or5
has been mapped, while QTL
or3.
2 was identified for the first time in the lower region of the same chromosome. Following the identification of major QTLs for resistance using the BSA-seq approach, all plants from three F
2
populations were genotyped using newly developed CAPS markers nearest to the QTL peak for
or3.
2, which confirmed the association of these markers with broomrape race G resistance. The results of this study will bring us a step closer to understanding the mechanisms underlying resistance of sunflower to highly virulent broomrape races, and the molecular markers developed herein will be highly useful for sunflower breeding programs.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11032-019-0948-9</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-1164-3664</orcidid></addata></record> |
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| subjects | Biomedical and Life Sciences Biotechnology Chromosome 3 Gene mapping Gene sequencing Genotyping Helianthus Life Sciences Mapping Markers Molecular biology Plant biology Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Population genetics Population studies Populations Quantitative trait loci |
| title | BSA-seq mapping reveals major QTL for broomrape resistance in four sunflower lines |
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