Improvement of kojic acid production in Aspergillus oryzae AR-47 mutant strain by combined mutagenesis
Kojic acid is a kind of secondary metabolites, whose biosynthesis pathway remains unclear to date. It is produced industrially by microbial fermentation, and thus, mutagenesis breeding still plays a vital role for obtaining strains with high kojic acid production. The starting strain KA-11 isolated...
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| Veröffentlicht in: | Bioprocess and biosystems engineering 2019-05, Vol.42 (5), p.753-761 |
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| creator | Feng, Weimeng Liang, Jinrui Wang, Bingbing Chen, Jianhua |
| description | Kojic acid is a kind of secondary metabolites, whose biosynthesis pathway remains unclear to date. It is produced industrially by microbial fermentation, and thus, mutagenesis breeding still plays a vital role for obtaining strains with high kojic acid production. The starting strain KA-11 isolated from mildewed fruits was identified as
Aspergillus oryzae
and then subjected to a combined mutagenesis program including microwave mutagenesis, UV irradiation, heat-LiCl, atmospheric, and room temperature plasma (ARTP). The kojic acid production was increased by 47.0%, 87.1%, 126.2%, and 292.3% compared with the starting strain KA-11 after each mutagenesis stage. A mutant strain AR-47 with kojic acid production of 96.5 g/L in flask-shake fermentation was finally obtained. The fermentation time also decreased from 7 to 5 days. Real-time quantitative PCR was used to determine the transcriptional expression levels of genes that may be relevant to kojic acid biosynthesis, including
kojA, kojR, kojT, AO090113000141, AO090113000143, AO090113000145, nrtA
, and
laeA
. The results showed that the transcriptional expression levels of all these genes in high yield strain AR-47 had increased compared with the starting strain KA-11. |
| doi_str_mv | 10.1007/s00449-019-02079-9 |
| format | Article |
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Aspergillus oryzae
and then subjected to a combined mutagenesis program including microwave mutagenesis, UV irradiation, heat-LiCl, atmospheric, and room temperature plasma (ARTP). The kojic acid production was increased by 47.0%, 87.1%, 126.2%, and 292.3% compared with the starting strain KA-11 after each mutagenesis stage. A mutant strain AR-47 with kojic acid production of 96.5 g/L in flask-shake fermentation was finally obtained. The fermentation time also decreased from 7 to 5 days. Real-time quantitative PCR was used to determine the transcriptional expression levels of genes that may be relevant to kojic acid biosynthesis, including
kojA, kojR, kojT, AO090113000141, AO090113000143, AO090113000145, nrtA
, and
laeA
. The results showed that the transcriptional expression levels of all these genes in high yield strain AR-47 had increased compared with the starting strain KA-11.</description><identifier>ISSN: 1615-7591</identifier><identifier>EISSN: 1615-7605</identifier><identifier>DOI: 10.1007/s00449-019-02079-9</identifier><identifier>PMID: 30805716</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acid production ; Acids ; Aspergillus oryzae ; Biosynthesis ; Biotechnology ; Chemistry ; Chemistry and Materials Science ; Environmental Engineering/Biotechnology ; Fermentation ; Food Science ; Gene expression ; Genes ; Industrial and Production Engineering ; Industrial Chemistry/Chemical Engineering ; Irradiation ; Kojic acid ; Lithium chloride ; Metabolites ; Microorganisms ; Mutagenesis ; Research Paper ; Secondary metabolites ; Strain ; Transcription ; Ultraviolet radiation</subject><ispartof>Bioprocess and biosystems engineering, 2019-05, Vol.42 (5), p.753-761</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2019</rights><rights>Bioprocess and Biosystems Engineering is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c522t-a59579ab395e53120821c932147f16f0a6a0c3327a9ba91cc8dbfb6e8052e8e3</citedby><cites>FETCH-LOGICAL-c522t-a59579ab395e53120821c932147f16f0a6a0c3327a9ba91cc8dbfb6e8052e8e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00449-019-02079-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00449-019-02079-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,782,786,27931,27932,41495,42564,51326</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30805716$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Feng, Weimeng</creatorcontrib><creatorcontrib>Liang, Jinrui</creatorcontrib><creatorcontrib>Wang, Bingbing</creatorcontrib><creatorcontrib>Chen, Jianhua</creatorcontrib><title>Improvement of kojic acid production in Aspergillus oryzae AR-47 mutant strain by combined mutagenesis</title><title>Bioprocess and biosystems engineering</title><addtitle>Bioprocess Biosyst Eng</addtitle><addtitle>Bioprocess Biosyst Eng</addtitle><description>Kojic acid is a kind of secondary metabolites, whose biosynthesis pathway remains unclear to date. It is produced industrially by microbial fermentation, and thus, mutagenesis breeding still plays a vital role for obtaining strains with high kojic acid production. The starting strain KA-11 isolated from mildewed fruits was identified as
Aspergillus oryzae
and then subjected to a combined mutagenesis program including microwave mutagenesis, UV irradiation, heat-LiCl, atmospheric, and room temperature plasma (ARTP). The kojic acid production was increased by 47.0%, 87.1%, 126.2%, and 292.3% compared with the starting strain KA-11 after each mutagenesis stage. A mutant strain AR-47 with kojic acid production of 96.5 g/L in flask-shake fermentation was finally obtained. The fermentation time also decreased from 7 to 5 days. Real-time quantitative PCR was used to determine the transcriptional expression levels of genes that may be relevant to kojic acid biosynthesis, including
kojA, kojR, kojT, AO090113000141, AO090113000143, AO090113000145, nrtA
, and
laeA
. The results showed that the transcriptional expression levels of all these genes in high yield strain AR-47 had increased compared with the starting strain KA-11.</description><subject>Acid production</subject><subject>Acids</subject><subject>Aspergillus oryzae</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Fermentation</subject><subject>Food Science</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Industrial and Production Engineering</subject><subject>Industrial Chemistry/Chemical Engineering</subject><subject>Irradiation</subject><subject>Kojic acid</subject><subject>Lithium chloride</subject><subject>Metabolites</subject><subject>Microorganisms</subject><subject>Mutagenesis</subject><subject>Research Paper</subject><subject>Secondary metabolites</subject><subject>Strain</subject><subject>Transcription</subject><subject>Ultraviolet radiation</subject><issn>1615-7591</issn><issn>1615-7605</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kF9LwzAUxYMobk6_gA8S8Ll6kzRN8ziGfwYDQfYe0jQdnWszk1aYn95s3fTNh5Bw7--ce3MQuiXwQADEYwBIU5kAiYeCkIk8Q2OSEZ6IDPj56c0lGaGrENYAhOcULtGIQQ5ckGyMqnmz9e7LNrbtsKvwh1vXBmtTlzjWy950tWtx3eJp2Fq_qjebPmDnd9_a4ul7kgrc9J2O2tB5HbFih41rirq15aGzsq0NdbhGF5XeBHtzvCdo-fy0nL0mi7eX-Wy6SAyntEs0l1xIXTDJLWeEQk6JkYySVFQkq0BnGgxjVGhZaEmMycuiKjIbf0NtbtkE3Q-2cffP3oZOrV3v2zhRUZJHayFBRooOlPEuBG8rtfV1o_1OEVD7ZNWQrIrJqkOyai-6O1r3RWPLX8kpygiwAQix1a6s_5v9j-0PhJeEEw</recordid><startdate>20190501</startdate><enddate>20190501</enddate><creator>Feng, Weimeng</creator><creator>Liang, Jinrui</creator><creator>Wang, Bingbing</creator><creator>Chen, Jianhua</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope></search><sort><creationdate>20190501</creationdate><title>Improvement of kojic acid production in Aspergillus oryzae AR-47 mutant strain by combined mutagenesis</title><author>Feng, Weimeng ; Liang, Jinrui ; Wang, Bingbing ; Chen, Jianhua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c522t-a59579ab395e53120821c932147f16f0a6a0c3327a9ba91cc8dbfb6e8052e8e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Acid production</topic><topic>Acids</topic><topic>Aspergillus oryzae</topic><topic>Biosynthesis</topic><topic>Biotechnology</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Environmental Engineering/Biotechnology</topic><topic>Fermentation</topic><topic>Food Science</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Industrial and Production Engineering</topic><topic>Industrial Chemistry/Chemical Engineering</topic><topic>Irradiation</topic><topic>Kojic acid</topic><topic>Lithium chloride</topic><topic>Metabolites</topic><topic>Microorganisms</topic><topic>Mutagenesis</topic><topic>Research Paper</topic><topic>Secondary metabolites</topic><topic>Strain</topic><topic>Transcription</topic><topic>Ultraviolet radiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Feng, Weimeng</creatorcontrib><creatorcontrib>Liang, Jinrui</creatorcontrib><creatorcontrib>Wang, Bingbing</creatorcontrib><creatorcontrib>Chen, Jianhua</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><jtitle>Bioprocess and biosystems engineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Feng, Weimeng</au><au>Liang, Jinrui</au><au>Wang, Bingbing</au><au>Chen, Jianhua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improvement of kojic acid production in Aspergillus oryzae AR-47 mutant strain by combined mutagenesis</atitle><jtitle>Bioprocess and biosystems engineering</jtitle><stitle>Bioprocess Biosyst Eng</stitle><addtitle>Bioprocess Biosyst Eng</addtitle><date>2019-05-01</date><risdate>2019</risdate><volume>42</volume><issue>5</issue><spage>753</spage><epage>761</epage><pages>753-761</pages><issn>1615-7591</issn><eissn>1615-7605</eissn><abstract>Kojic acid is a kind of secondary metabolites, whose biosynthesis pathway remains unclear to date. It is produced industrially by microbial fermentation, and thus, mutagenesis breeding still plays a vital role for obtaining strains with high kojic acid production. The starting strain KA-11 isolated from mildewed fruits was identified as
Aspergillus oryzae
and then subjected to a combined mutagenesis program including microwave mutagenesis, UV irradiation, heat-LiCl, atmospheric, and room temperature plasma (ARTP). The kojic acid production was increased by 47.0%, 87.1%, 126.2%, and 292.3% compared with the starting strain KA-11 after each mutagenesis stage. A mutant strain AR-47 with kojic acid production of 96.5 g/L in flask-shake fermentation was finally obtained. The fermentation time also decreased from 7 to 5 days. Real-time quantitative PCR was used to determine the transcriptional expression levels of genes that may be relevant to kojic acid biosynthesis, including
kojA, kojR, kojT, AO090113000141, AO090113000143, AO090113000145, nrtA
, and
laeA
. The results showed that the transcriptional expression levels of all these genes in high yield strain AR-47 had increased compared with the starting strain KA-11.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>30805716</pmid><doi>10.1007/s00449-019-02079-9</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | SpringerNature Journals |
| subjects | Acid production Acids Aspergillus oryzae Biosynthesis Biotechnology Chemistry Chemistry and Materials Science Environmental Engineering/Biotechnology Fermentation Food Science Gene expression Genes Industrial and Production Engineering Industrial Chemistry/Chemical Engineering Irradiation Kojic acid Lithium chloride Metabolites Microorganisms Mutagenesis Research Paper Secondary metabolites Strain Transcription Ultraviolet radiation |
| title | Improvement of kojic acid production in Aspergillus oryzae AR-47 mutant strain by combined mutagenesis |
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