Isolation of two novel promoters from ramie (Boehmeria nivea L. Gaudich) and its functional characterization in Arabidopsis thaliana
The development of tissue-specific or inducible promoters is important for plant genetic engineering. In this study, we isolated two novel promoters (named BnGDS pro and BnSTM pro ) from ramie and analyzed their functions in Arabidopsis . The results show that BnGDS pro and its 5′-truncated fragment...
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| Veröffentlicht in: | Plant cell, tissue and organ culture tissue and organ culture, 2019-03, Vol.136 (3), p.467-478 |
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| container_title | Plant cell, tissue and organ culture |
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| creator | Guo, Pingan Zheng, Yancheng Chen, Jie Wang, Bo Liu, Lijun Feng, Enying Peng, Dingxiang |
| description | The development of tissue-specific or inducible promoters is important for plant genetic engineering. In this study, we isolated two novel promoters (named
BnGDS
pro
and
BnSTM
pro
) from ramie and analyzed their functions in
Arabidopsis
. The results show that
BnGDS
pro
and its 5′-truncated fragments drive GUS dominantly expressed in shoot meristem while
BnSTM
pro
and its 5′-truncated fragments have apical meristem specificity in
Arabidopsis
seedlings and nodule specificity in the flowers and siliques. The − 1233 to − 914 region and − 425 to 0 region of
BnSTM
pro
are responsible for apical root specificity and root-knot specificity, respectively. In particular, CuSO
4
treatment reveals that both
BnGDS
pro
and
BnSTM
pro
are copper-inducible promoters. Our findings suggest that
BnGDS
pro
and
BnSTM
pro
and their truncated fragments have potential applications in plant genetic transformation. |
| doi_str_mv | 10.1007/s11240-018-01528-9 |
| format | Article |
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BnGDS
pro
and
BnSTM
pro
) from ramie and analyzed their functions in
Arabidopsis
. The results show that
BnGDS
pro
and its 5′-truncated fragments drive GUS dominantly expressed in shoot meristem while
BnSTM
pro
and its 5′-truncated fragments have apical meristem specificity in
Arabidopsis
seedlings and nodule specificity in the flowers and siliques. The − 1233 to − 914 region and − 425 to 0 region of
BnSTM
pro
are responsible for apical root specificity and root-knot specificity, respectively. In particular, CuSO
4
treatment reveals that both
BnGDS
pro
and
BnSTM
pro
are copper-inducible promoters. Our findings suggest that
BnGDS
pro
and
BnSTM
pro
and their truncated fragments have potential applications in plant genetic transformation.</description><identifier>ISSN: 0167-6857</identifier><identifier>EISSN: 1573-5044</identifier><identifier>DOI: 10.1007/s11240-018-01528-9</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Arabidopsis ; Biomedical and Life Sciences ; Flowers ; Fragmentation ; Fragments ; Genetic engineering ; Genetic transformation ; Life Sciences ; Nodules ; Original Article ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Promoters ; Seedlings</subject><ispartof>Plant cell, tissue and organ culture, 2019-03, Vol.136 (3), p.467-478</ispartof><rights>Springer Nature B.V. 2018</rights><rights>Copyright Springer Nature B.V. 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-3508fa0cf08c96ac7bbfce7710eac07cbc4d13f1e857f4a8ac6696c04f1af52f3</citedby><cites>FETCH-LOGICAL-c319t-3508fa0cf08c96ac7bbfce7710eac07cbc4d13f1e857f4a8ac6696c04f1af52f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11240-018-01528-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11240-018-01528-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Guo, Pingan</creatorcontrib><creatorcontrib>Zheng, Yancheng</creatorcontrib><creatorcontrib>Chen, Jie</creatorcontrib><creatorcontrib>Wang, Bo</creatorcontrib><creatorcontrib>Liu, Lijun</creatorcontrib><creatorcontrib>Feng, Enying</creatorcontrib><creatorcontrib>Peng, Dingxiang</creatorcontrib><title>Isolation of two novel promoters from ramie (Boehmeria nivea L. Gaudich) and its functional characterization in Arabidopsis thaliana</title><title>Plant cell, tissue and organ culture</title><addtitle>Plant Cell Tiss Organ Cult</addtitle><description>The development of tissue-specific or inducible promoters is important for plant genetic engineering. In this study, we isolated two novel promoters (named
BnGDS
pro
and
BnSTM
pro
) from ramie and analyzed their functions in
Arabidopsis
. The results show that
BnGDS
pro
and its 5′-truncated fragments drive GUS dominantly expressed in shoot meristem while
BnSTM
pro
and its 5′-truncated fragments have apical meristem specificity in
Arabidopsis
seedlings and nodule specificity in the flowers and siliques. The − 1233 to − 914 region and − 425 to 0 region of
BnSTM
pro
are responsible for apical root specificity and root-knot specificity, respectively. In particular, CuSO
4
treatment reveals that both
BnGDS
pro
and
BnSTM
pro
are copper-inducible promoters. Our findings suggest that
BnGDS
pro
and
BnSTM
pro
and their truncated fragments have potential applications in plant genetic transformation.</description><subject>Arabidopsis</subject><subject>Biomedical and Life Sciences</subject><subject>Flowers</subject><subject>Fragmentation</subject><subject>Fragments</subject><subject>Genetic engineering</subject><subject>Genetic transformation</subject><subject>Life Sciences</subject><subject>Nodules</subject><subject>Original Article</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Promoters</subject><subject>Seedlings</subject><issn>0167-6857</issn><issn>1573-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kD9PwzAQxS0EEqXwBZgsscCQcs4_J2OpoFSqxAKzdXFs4iqNi50UwcwHxyVIbAynu-G9d3c_Qi4ZzBgAv_WMxSlEwIpQWVxE5RGZsIwnUQZpekwmwHIe5UXGT8mZ9xsAyJOUTcjXytsWe2M7ajXt3y3t7F61dOfs1vbKearDRB1ujaLXd1Y1W-UM0s7sFdL1jC5xqI1sbih2NTV90A-dPORhS2WDDmVIMZ_jCtPRucPK1Hbnjad9g63BDs_JicbWq4vfPiUvD_fPi8do_bRcLebrSCas7KMkg0IjSA2FLHOUvKq0VJwzUCiBy0qmNUs0U-FNnWKBMs_LXEKqGeos1smUXI254bu3QflebOzgwqVexKxIeFwEeEEVjyrprPdOabFzZovuQzAQB9pipC0CbfFDW5TBlIwmH8Tdq3J_0f-4vgHUqIUL</recordid><startdate>20190315</startdate><enddate>20190315</enddate><creator>Guo, Pingan</creator><creator>Zheng, Yancheng</creator><creator>Chen, Jie</creator><creator>Wang, Bo</creator><creator>Liu, Lijun</creator><creator>Feng, Enying</creator><creator>Peng, Dingxiang</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20190315</creationdate><title>Isolation of two novel promoters from ramie (Boehmeria nivea L. Gaudich) and its functional characterization in Arabidopsis thaliana</title><author>Guo, Pingan ; Zheng, Yancheng ; Chen, Jie ; Wang, Bo ; Liu, Lijun ; Feng, Enying ; Peng, Dingxiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-3508fa0cf08c96ac7bbfce7710eac07cbc4d13f1e857f4a8ac6696c04f1af52f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Arabidopsis</topic><topic>Biomedical and Life Sciences</topic><topic>Flowers</topic><topic>Fragmentation</topic><topic>Fragments</topic><topic>Genetic engineering</topic><topic>Genetic transformation</topic><topic>Life Sciences</topic><topic>Nodules</topic><topic>Original Article</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Promoters</topic><topic>Seedlings</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guo, Pingan</creatorcontrib><creatorcontrib>Zheng, Yancheng</creatorcontrib><creatorcontrib>Chen, Jie</creatorcontrib><creatorcontrib>Wang, Bo</creatorcontrib><creatorcontrib>Liu, Lijun</creatorcontrib><creatorcontrib>Feng, Enying</creatorcontrib><creatorcontrib>Peng, Dingxiang</creatorcontrib><collection>CrossRef</collection><jtitle>Plant cell, tissue and organ culture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guo, Pingan</au><au>Zheng, Yancheng</au><au>Chen, Jie</au><au>Wang, Bo</au><au>Liu, Lijun</au><au>Feng, Enying</au><au>Peng, Dingxiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of two novel promoters from ramie (Boehmeria nivea L. Gaudich) and its functional characterization in Arabidopsis thaliana</atitle><jtitle>Plant cell, tissue and organ culture</jtitle><stitle>Plant Cell Tiss Organ Cult</stitle><date>2019-03-15</date><risdate>2019</risdate><volume>136</volume><issue>3</issue><spage>467</spage><epage>478</epage><pages>467-478</pages><issn>0167-6857</issn><eissn>1573-5044</eissn><abstract>The development of tissue-specific or inducible promoters is important for plant genetic engineering. In this study, we isolated two novel promoters (named
BnGDS
pro
and
BnSTM
pro
) from ramie and analyzed their functions in
Arabidopsis
. The results show that
BnGDS
pro
and its 5′-truncated fragments drive GUS dominantly expressed in shoot meristem while
BnSTM
pro
and its 5′-truncated fragments have apical meristem specificity in
Arabidopsis
seedlings and nodule specificity in the flowers and siliques. The − 1233 to − 914 region and − 425 to 0 region of
BnSTM
pro
are responsible for apical root specificity and root-knot specificity, respectively. In particular, CuSO
4
treatment reveals that both
BnGDS
pro
and
BnSTM
pro
are copper-inducible promoters. Our findings suggest that
BnGDS
pro
and
BnSTM
pro
and their truncated fragments have potential applications in plant genetic transformation.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11240-018-01528-9</doi><tpages>12</tpages></addata></record> |
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| ispartof | Plant cell, tissue and organ culture, 2019-03, Vol.136 (3), p.467-478 |
| issn | 0167-6857 1573-5044 |
| language | eng |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | Arabidopsis Biomedical and Life Sciences Flowers Fragmentation Fragments Genetic engineering Genetic transformation Life Sciences Nodules Original Article Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Promoters Seedlings |
| title | Isolation of two novel promoters from ramie (Boehmeria nivea L. Gaudich) and its functional characterization in Arabidopsis thaliana |
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