Combined effects of graphene oxide and zinc oxide nanoparticle on human A549 cells: bioavailability, toxicity and mechanisms
The toxic effects of multinanomaterial systems are receiving more attention due to their release of various nanomaterials. However, the knowledge of the influence of two-dimensional carbon nanomaterials on the bioavailability and combined toxicity of metal oxide nanoparticles in human cells is limit...
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| Veröffentlicht in: | Environmental science. Nano 2019-02, Vol.6 (2), p.635-645 |
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| creator | Wu, Bing Wu, Jialu Liu, Su Shen, Zhuoyan Chen, Ling Zhang, Xu-Xiang Ren, Hong-qiang |
| description | The toxic effects of multinanomaterial systems are receiving more attention due to their release of various nanomaterials. However, the knowledge of the influence of two-dimensional carbon nanomaterials on the bioavailability and combined toxicity of metal oxide nanoparticles in human cells is limited. In this study, we analyzed the interaction and combined toxicity of graphene oxide (GO) and zinc oxide nanoparticles (nano-ZnO) in the human lung carcinoma epithelial A549 cell line. The results showed that GO (1, 5 and 10 mg L
−1
) did not change the precipitation and Zn
2+
release of nano-ZnO in the cell culture medium and had low adsorption capability to Zn
2+
. However, GO could reduce the bioavailability and toxicity of nano-ZnO in cell viability, oxidative stress, mitochondrial depolarization, and membrane damage. The metabolomics analysis showed that exposure to nano-ZnO alone and coexposure to both nanomaterials significantly changed the metabolome profiles and had higher similar impacts on tricarboxylic acid cycle, glutathione synthesis, nucleoside synthesis and lipid metabolism. However, GO reduced the impact of nano-ZnO upon fold changes of the most altered metabolites. Furthermore, in this study, we found that GO increased the toxicity of Zn
2+
, which differed from the effects of GO on nano-ZnO. This difference might be due to different modes of action, such that GO decreased the uptake of nano-ZnO, but inhibited the efflux of Zn
2+
in cells. The results of this study provided insights into the combined toxicity evaluation of GO and metal oxide nanoparticles.
The toxic effects of multinanomaterial systems are receiving more attention due to their release of various nanomaterials. |
| doi_str_mv | 10.1039/c8en00965a |
| format | Article |
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−1
) did not change the precipitation and Zn
2+
release of nano-ZnO in the cell culture medium and had low adsorption capability to Zn
2+
. However, GO could reduce the bioavailability and toxicity of nano-ZnO in cell viability, oxidative stress, mitochondrial depolarization, and membrane damage. The metabolomics analysis showed that exposure to nano-ZnO alone and coexposure to both nanomaterials significantly changed the metabolome profiles and had higher similar impacts on tricarboxylic acid cycle, glutathione synthesis, nucleoside synthesis and lipid metabolism. However, GO reduced the impact of nano-ZnO upon fold changes of the most altered metabolites. Furthermore, in this study, we found that GO increased the toxicity of Zn
2+
, which differed from the effects of GO on nano-ZnO. This difference might be due to different modes of action, such that GO decreased the uptake of nano-ZnO, but inhibited the efflux of Zn
2+
in cells. The results of this study provided insights into the combined toxicity evaluation of GO and metal oxide nanoparticles.
The toxic effects of multinanomaterial systems are receiving more attention due to their release of various nanomaterials.</description><identifier>ISSN: 2051-8153</identifier><identifier>EISSN: 2051-8161</identifier><identifier>DOI: 10.1039/c8en00965a</identifier><language>eng</language><publisher>Cambridge: Royal Society of Chemistry</publisher><subject>Bioavailability ; Cell culture ; Cells ; Culture media ; Depolarization ; Efflux ; Evaluation ; Glutathione ; Graphene ; Heavy metals ; Lipid metabolism ; Lipids ; Lung carcinoma ; Lungs ; Membrane potential ; Metabolism ; Metabolites ; Metabolomics ; Metal oxides ; Metals ; Mitochondria ; Nanomaterials ; Nanoparticles ; Nanotechnology ; Neoplasms ; Oxidative stress ; Profiles ; Synthesis ; Toxicity ; Tricarboxylic acid cycle ; Uptake ; Zinc ; Zinc oxide ; Zinc oxides</subject><ispartof>Environmental science. Nano, 2019-02, Vol.6 (2), p.635-645</ispartof><rights>Copyright Royal Society of Chemistry 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c281t-f07057d8e7779945b7574d1ed5454cb9214465b4a0bf2e17fa4a2c240bbab4e43</citedby><cites>FETCH-LOGICAL-c281t-f07057d8e7779945b7574d1ed5454cb9214465b4a0bf2e17fa4a2c240bbab4e43</cites><orcidid>0000-0002-2833-0656 ; 0000-0001-7117-580X ; 0000-0002-6434-692X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Wu, Bing</creatorcontrib><creatorcontrib>Wu, Jialu</creatorcontrib><creatorcontrib>Liu, Su</creatorcontrib><creatorcontrib>Shen, Zhuoyan</creatorcontrib><creatorcontrib>Chen, Ling</creatorcontrib><creatorcontrib>Zhang, Xu-Xiang</creatorcontrib><creatorcontrib>Ren, Hong-qiang</creatorcontrib><title>Combined effects of graphene oxide and zinc oxide nanoparticle on human A549 cells: bioavailability, toxicity and mechanisms</title><title>Environmental science. Nano</title><description>The toxic effects of multinanomaterial systems are receiving more attention due to their release of various nanomaterials. However, the knowledge of the influence of two-dimensional carbon nanomaterials on the bioavailability and combined toxicity of metal oxide nanoparticles in human cells is limited. In this study, we analyzed the interaction and combined toxicity of graphene oxide (GO) and zinc oxide nanoparticles (nano-ZnO) in the human lung carcinoma epithelial A549 cell line. The results showed that GO (1, 5 and 10 mg L
−1
) did not change the precipitation and Zn
2+
release of nano-ZnO in the cell culture medium and had low adsorption capability to Zn
2+
. However, GO could reduce the bioavailability and toxicity of nano-ZnO in cell viability, oxidative stress, mitochondrial depolarization, and membrane damage. The metabolomics analysis showed that exposure to nano-ZnO alone and coexposure to both nanomaterials significantly changed the metabolome profiles and had higher similar impacts on tricarboxylic acid cycle, glutathione synthesis, nucleoside synthesis and lipid metabolism. However, GO reduced the impact of nano-ZnO upon fold changes of the most altered metabolites. Furthermore, in this study, we found that GO increased the toxicity of Zn
2+
, which differed from the effects of GO on nano-ZnO. This difference might be due to different modes of action, such that GO decreased the uptake of nano-ZnO, but inhibited the efflux of Zn
2+
in cells. The results of this study provided insights into the combined toxicity evaluation of GO and metal oxide nanoparticles.
The toxic effects of multinanomaterial systems are receiving more attention due to their release of various nanomaterials.</description><subject>Bioavailability</subject><subject>Cell culture</subject><subject>Cells</subject><subject>Culture media</subject><subject>Depolarization</subject><subject>Efflux</subject><subject>Evaluation</subject><subject>Glutathione</subject><subject>Graphene</subject><subject>Heavy metals</subject><subject>Lipid metabolism</subject><subject>Lipids</subject><subject>Lung carcinoma</subject><subject>Lungs</subject><subject>Membrane potential</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Metabolomics</subject><subject>Metal oxides</subject><subject>Metals</subject><subject>Mitochondria</subject><subject>Nanomaterials</subject><subject>Nanoparticles</subject><subject>Nanotechnology</subject><subject>Neoplasms</subject><subject>Oxidative stress</subject><subject>Profiles</subject><subject>Synthesis</subject><subject>Toxicity</subject><subject>Tricarboxylic acid cycle</subject><subject>Uptake</subject><subject>Zinc</subject><subject>Zinc oxide</subject><subject>Zinc oxides</subject><issn>2051-8153</issn><issn>2051-8161</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNpFkd1LwzAUxYMoOOZefBcCvonVpE2a1rdR5gcMfdHncpMmLqNNZtKJE_946zbm0z0XfvdcOAehc0puKMnKW1VoR0iZczhCo5RwmhQ0p8cHzbNTNIlxSQihNOVZLkbop_KdtE43WBujVR-xN_g9wGqhncb-yzYag2vwt3VqvzpwfgWht6odCIcX6w4cnnJWYqXbNt5haT18gm1B2tb2m2vcD5dqUFurTqsFOBu7eIZODLRRT_ZzjN7uZ6_VYzJ_eXiqpvNEpQXtE0ME4aIptBCiLBmXggvWUN1wxpmSZUoZy7lkQKRJNRUGGKQqZURKkEyzbIwud76r4D_WOvb10q-DG17WKRWlEIXg-UBd7SgVfIxBm3oVbAdhU1NS_wVcV8XseRvwdIAvdnCI6sD9F5D9Al3oeAE</recordid><startdate>20190214</startdate><enddate>20190214</enddate><creator>Wu, Bing</creator><creator>Wu, Jialu</creator><creator>Liu, Su</creator><creator>Shen, Zhuoyan</creator><creator>Chen, Ling</creator><creator>Zhang, Xu-Xiang</creator><creator>Ren, Hong-qiang</creator><general>Royal Society of Chemistry</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7ST</scope><scope>7UA</scope><scope>C1K</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope><scope>SOI</scope><orcidid>https://orcid.org/0000-0002-2833-0656</orcidid><orcidid>https://orcid.org/0000-0001-7117-580X</orcidid><orcidid>https://orcid.org/0000-0002-6434-692X</orcidid></search><sort><creationdate>20190214</creationdate><title>Combined effects of graphene oxide and zinc oxide nanoparticle on human A549 cells: bioavailability, toxicity and mechanisms</title><author>Wu, Bing ; Wu, Jialu ; Liu, Su ; Shen, Zhuoyan ; Chen, Ling ; Zhang, Xu-Xiang ; Ren, Hong-qiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c281t-f07057d8e7779945b7574d1ed5454cb9214465b4a0bf2e17fa4a2c240bbab4e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Bioavailability</topic><topic>Cell culture</topic><topic>Cells</topic><topic>Culture media</topic><topic>Depolarization</topic><topic>Efflux</topic><topic>Evaluation</topic><topic>Glutathione</topic><topic>Graphene</topic><topic>Heavy metals</topic><topic>Lipid metabolism</topic><topic>Lipids</topic><topic>Lung carcinoma</topic><topic>Lungs</topic><topic>Membrane potential</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Metabolomics</topic><topic>Metal oxides</topic><topic>Metals</topic><topic>Mitochondria</topic><topic>Nanomaterials</topic><topic>Nanoparticles</topic><topic>Nanotechnology</topic><topic>Neoplasms</topic><topic>Oxidative stress</topic><topic>Profiles</topic><topic>Synthesis</topic><topic>Toxicity</topic><topic>Tricarboxylic acid cycle</topic><topic>Uptake</topic><topic>Zinc</topic><topic>Zinc oxide</topic><topic>Zinc oxides</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Bing</creatorcontrib><creatorcontrib>Wu, Jialu</creatorcontrib><creatorcontrib>Liu, Su</creatorcontrib><creatorcontrib>Shen, Zhuoyan</creatorcontrib><creatorcontrib>Chen, Ling</creatorcontrib><creatorcontrib>Zhang, Xu-Xiang</creatorcontrib><creatorcontrib>Ren, Hong-qiang</creatorcontrib><collection>CrossRef</collection><collection>Aqualine</collection><collection>Environment Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Environment Abstracts</collection><jtitle>Environmental science. Nano</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Bing</au><au>Wu, Jialu</au><au>Liu, Su</au><au>Shen, Zhuoyan</au><au>Chen, Ling</au><au>Zhang, Xu-Xiang</au><au>Ren, Hong-qiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Combined effects of graphene oxide and zinc oxide nanoparticle on human A549 cells: bioavailability, toxicity and mechanisms</atitle><jtitle>Environmental science. Nano</jtitle><date>2019-02-14</date><risdate>2019</risdate><volume>6</volume><issue>2</issue><spage>635</spage><epage>645</epage><pages>635-645</pages><issn>2051-8153</issn><eissn>2051-8161</eissn><abstract>The toxic effects of multinanomaterial systems are receiving more attention due to their release of various nanomaterials. However, the knowledge of the influence of two-dimensional carbon nanomaterials on the bioavailability and combined toxicity of metal oxide nanoparticles in human cells is limited. In this study, we analyzed the interaction and combined toxicity of graphene oxide (GO) and zinc oxide nanoparticles (nano-ZnO) in the human lung carcinoma epithelial A549 cell line. The results showed that GO (1, 5 and 10 mg L
−1
) did not change the precipitation and Zn
2+
release of nano-ZnO in the cell culture medium and had low adsorption capability to Zn
2+
. However, GO could reduce the bioavailability and toxicity of nano-ZnO in cell viability, oxidative stress, mitochondrial depolarization, and membrane damage. The metabolomics analysis showed that exposure to nano-ZnO alone and coexposure to both nanomaterials significantly changed the metabolome profiles and had higher similar impacts on tricarboxylic acid cycle, glutathione synthesis, nucleoside synthesis and lipid metabolism. However, GO reduced the impact of nano-ZnO upon fold changes of the most altered metabolites. Furthermore, in this study, we found that GO increased the toxicity of Zn
2+
, which differed from the effects of GO on nano-ZnO. This difference might be due to different modes of action, such that GO decreased the uptake of nano-ZnO, but inhibited the efflux of Zn
2+
in cells. The results of this study provided insights into the combined toxicity evaluation of GO and metal oxide nanoparticles.
The toxic effects of multinanomaterial systems are receiving more attention due to their release of various nanomaterials.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><doi>10.1039/c8en00965a</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-2833-0656</orcidid><orcidid>https://orcid.org/0000-0001-7117-580X</orcidid><orcidid>https://orcid.org/0000-0002-6434-692X</orcidid></addata></record> |
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| source | Royal Society Of Chemistry Journals |
| subjects | Bioavailability Cell culture Cells Culture media Depolarization Efflux Evaluation Glutathione Graphene Heavy metals Lipid metabolism Lipids Lung carcinoma Lungs Membrane potential Metabolism Metabolites Metabolomics Metal oxides Metals Mitochondria Nanomaterials Nanoparticles Nanotechnology Neoplasms Oxidative stress Profiles Synthesis Toxicity Tricarboxylic acid cycle Uptake Zinc Zinc oxide Zinc oxides |
| title | Combined effects of graphene oxide and zinc oxide nanoparticle on human A549 cells: bioavailability, toxicity and mechanisms |
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