Double-Tagging Polymerase Chain Reaction with a Thiolated Primer and Electrochemical Genosensing based on Gold Nanocomposite Sensor for Food Safety

A novel material for electrochemical biosensing based on rigid conducting gold nanocomposite (nano-AuGEC) is presented. Islands of chemisorbing material (gold nanoparticles) surrounded by nonreactive, rigid, and conducting graphite epoxy composite are thus achieved to avoid the stringent control of...

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Veröffentlicht in:	Analytical chemistry (Washington) 2009-02, Vol.81 (4), p.1332-1339
Hauptverfasser:	Brasil de Oliveira Marques, Paulo R, Lermo, Anabel, Campoy, Susana, Yamanaka, Hideko, Barbé, Jordi, Alegret, Salvador, Pividori, M. Isabel
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Animals Base Sequence Biological and medical sciences Biosensing Techniques - methods Biosensors Biotechnology Chemistry Deoxyribonucleic acid Digoxigenin - chemistry Digoxigenin - metabolism DNA DNA Primers - genetics DNA Primers - metabolism DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Bacterial - metabolism Electrochemical methods Electrochemistry Electrodes Exact sciences and technology Food Analysis - methods Food Microbiology Food safety Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Genome, Bacterial Gold Gold - chemistry In vitro gene amplification. Pcr technique Methods. Procedures. Technologies Nanocomposites Nanocomposites - chemistry Nucleic Acid Hybridization Polymerase Chain Reaction Salmonella Salmonella - genetics Salmonella - isolation & purification Sulfhydryl Compounds - chemistry Surface Properties Time Factors Various methods and equipments
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