Noncompetitive Immunoassay of Small Analytes at the Femtomolar Level by Affinity Probe Capillary Electrophoresis: Direct Analysis of Digoxin Using a Uniform-Labeled scFv Immunoreagent

A general method for noncompetitive immunoassay of small analytes using affinity probe capillary electrophoresis (APCE) is demonstrated using digoxin as a model analyte. A uniform immunoreagent was prepared from a single-chain antibody (scFv) gene specific for digoxin. Site-directed mutagenesis intr...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Analytical chemistry (Washington) 2000-12, Vol.72 (23), p.5779-5786
Hauptverfasser:	Hafner, Frank T, Kautz, Roger A, Iverson, Brent L, Tim, Roger C, Karger, Barry L
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity Labels Analysis Analytical biochemistry: general aspects, technics, instrumentation Analytical, structural and metabolic biochemistry Biological and medical sciences Cardiotonic Agents - analysis Cardiotonic Agents - blood Cardiotonic Agents - urine Chemistry Chromatography, High Pressure Liquid Digoxin - analysis Digoxin - blood Digoxin - urine Electrophoresis, Capillary Fundamental and applied biological sciences. Psychology Humans Immunoassay Immunoglobulin Fab Fragments Reproducibility of Results Sensitivity and Specificity Spectrophotometry, Ultraviolet
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!