Mediated Bioelectrocatalytic Determination of Organophosphorus Pesticides with a Tyrosinase-Based Oxygen Biosensor
An electrochemical biosensor for the detection of organophosphorus pesticides is described. A tyrosinase-based oxygen sensor is constructed where enzymatic oxygen consumption is monitored electrochemically with the mediator 1,2-naphthoquinone-4-sulfonate. This bioelectrocatalytic system allows elect...
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| Veröffentlicht in: | Analytical chemistry (Washington) 1998-02, Vol.70 (4), p.807-810 |
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| container_title | Analytical chemistry (Washington) |
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| creator | Everett, W. Russell Rechnitz, Garry A. |
| description | An electrochemical biosensor for the detection of organophosphorus pesticides is described. A tyrosinase-based oxygen sensor is constructed where enzymatic oxygen consumption is monitored electrochemically with the mediator 1,2-naphthoquinone-4-sulfonate. This bioelectrocatalytic system allows electrochemical initiation and termination of the enzymatic reaction. Amperometric and coulometric techniques are used to study inhibitory effects for pesticide determinations. This inhibition appears to be fully reversible, with full catalytic activity returning after removal of the pesticides. Detection limits are 5 μM for diazinon and 75 nM for dichlorvos. Enzymatic inhibition of 50% occurs at ∼1000 and ∼50 μM, respectively. |
| doi_str_mv | 10.1021/ac970958l |
| format | Article |
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Enzymatic inhibition of 50% occurs at ∼1000 and ∼50 μM, respectively.</description><subject>Biological and medical sciences</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Chemistry</subject><subject>Enzymes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Methods. Procedures. 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| source | American Chemical Society Journals |
| subjects | Biological and medical sciences Biosensors Biotechnology Chemistry Enzymes Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Pesticides Various methods and equipments |
| title | Mediated Bioelectrocatalytic Determination of Organophosphorus Pesticides with a Tyrosinase-Based Oxygen Biosensor |
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