A Monoclonal Immunoassay for the Coplanar Polychlorinated Biphenyls
Polychlorinated biphenyls (PCBs) are ubiquitous environmental pollutants with diverse toxic, teratogenic, reproductive, immunotoxic, and tumorigenic effects. Three of the least abundant of the 209 PCB isomers (congeners) are the most toxic and most difficult to quantify. These are 3,4,3',4'...
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| Veröffentlicht in: | Analytical chemistry (Washington) 1995-11, Vol.67 (21), p.3829-3839 |
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| creator | Chiu, Ya-Wen Carlson, Robert E Marcus, Karen L Karu, Alexander E |
| description | Polychlorinated biphenyls (PCBs) are ubiquitous environmental pollutants with diverse toxic, teratogenic, reproductive, immunotoxic, and tumorigenic effects. Three of the least abundant of the 209 PCB isomers (congeners) are the most toxic and most difficult to quantify. These are 3,4,3',4'-tetrachlorobiphenyl, 3,4,3',4',5'-pentachlorobiphenyl, and 3,4,5,3',4',5'-hexachlorobiphenyl (IU-PAC No. 77, 126, and 169, respectively). An immunizing hapten was designed to retain the 3,4,3',4' chlorine-substitution pattern and coplanarity characteristic of these toxic congeners. The optimal competitors for immunoassay were weaker binding distinctive single-ring fragments of the PCBs. A monoclonal antibody designated S2B1 was derived and used in direct (antibody-capture) competitive enzyme immunoassays (EIAs). The EIAs are highly specific for non-ortho-substituted congeners and do not recognize the more prevalent but much less toxic noncoplanar PCB congeners or 2,3,7,8-tetrachlorodibenzo-p-dioxin, 2,3,7,8-tetrachlorodibenzofuran, or dichlorobenzenes. Hapten and competitor design for this assay suggests a basis for development of sensitive EIAs for other classes of PCB congeners. |
| doi_str_mv | 10.1021/ac00117a003 |
| format | Article |
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Three of the least abundant of the 209 PCB isomers (congeners) are the most toxic and most difficult to quantify. These are 3,4,3',4'-tetrachlorobiphenyl, 3,4,3',4',5'-pentachlorobiphenyl, and 3,4,5,3',4',5'-hexachlorobiphenyl (IU-PAC No. 77, 126, and 169, respectively). An immunizing hapten was designed to retain the 3,4,3',4' chlorine-substitution pattern and coplanarity characteristic of these toxic congeners. The optimal competitors for immunoassay were weaker binding distinctive single-ring fragments of the PCBs. A monoclonal antibody designated S2B1 was derived and used in direct (antibody-capture) competitive enzyme immunoassays (EIAs). The EIAs are highly specific for non-ortho-substituted congeners and do not recognize the more prevalent but much less toxic noncoplanar PCB congeners or 2,3,7,8-tetrachlorodibenzo-p-dioxin, 2,3,7,8-tetrachlorodibenzofuran, or dichlorobenzenes. 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Chem</addtitle><description>Polychlorinated biphenyls (PCBs) are ubiquitous environmental pollutants with diverse toxic, teratogenic, reproductive, immunotoxic, and tumorigenic effects. Three of the least abundant of the 209 PCB isomers (congeners) are the most toxic and most difficult to quantify. These are 3,4,3',4'-tetrachlorobiphenyl, 3,4,3',4',5'-pentachlorobiphenyl, and 3,4,5,3',4',5'-hexachlorobiphenyl (IU-PAC No. 77, 126, and 169, respectively). An immunizing hapten was designed to retain the 3,4,3',4' chlorine-substitution pattern and coplanarity characteristic of these toxic congeners. The optimal competitors for immunoassay were weaker binding distinctive single-ring fragments of the PCBs. A monoclonal antibody designated S2B1 was derived and used in direct (antibody-capture) competitive enzyme immunoassays (EIAs). The EIAs are highly specific for non-ortho-substituted congeners and do not recognize the more prevalent but much less toxic noncoplanar PCB congeners or 2,3,7,8-tetrachlorodibenzo-p-dioxin, 2,3,7,8-tetrachlorodibenzofuran, or dichlorobenzenes. Hapten and competitor design for this assay suggests a basis for development of sensitive EIAs for other classes of PCB congeners.</description><subject>Analytical chemistry</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Binding, Competitive</subject><subject>Cells, Cultured</subject><subject>Chemistry</subject><subject>Cross Reactions</subject><subject>Exact sciences and technology</subject><subject>Haptens - chemistry</subject><subject>Hybridomas</subject><subject>Immunoenzyme Techniques</subject><subject>Mice</subject><subject>Miscellaneous</subject><subject>PCB</subject><subject>Polychlorinated biphenyls</subject><subject>Polychlorinated Biphenyls - analysis</subject><subject>Polychlorinated Biphenyls - metabolism</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0M9LwzAUB_AgypzTk2ehiOBBqvmxJtlxDqfCxIHTa3hNU9bZNTVpwf33ZnRMD54C73348vJF6JzgW4IpuQONMSECMGYHqE8SimMuJT1EfRxGMRUYH6MT71dbhgnvoZ7kjIlk2EeTcfRiK6tLW0EZPa_XbWXBe9hEuXVRszTRxNYlVOCiuS03ellaV1TQmCy6L-qlqTalP0VHOZTenO3eAXqfPiwmT_Hs9fF5Mp7FMBS8iZOU80zqlCSJkJmRbJjkAnLGCDXS4BGnmcZAZZ5qwWCkszSVw5GgLBE541yzAbrscmtnv1rjG7WyrQt3e0WJCBazUUA3HdLOeu9MrmpXrMFtFMFqW5f6U1fQF7vINl2bbG93_YT91W4PXkOZO6h04feMCUxEwgOLO1b4xnzv1-A-FRchSC3mb4rO2EfInKpp8NedB-1_P_HfgT-nz4vR</recordid><startdate>19951101</startdate><enddate>19951101</enddate><creator>Chiu, Ya-Wen</creator><creator>Carlson, Robert E</creator><creator>Marcus, Karen L</creator><creator>Karu, Alexander E</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope></search><sort><creationdate>19951101</creationdate><title>A Monoclonal Immunoassay for the Coplanar Polychlorinated Biphenyls</title><author>Chiu, Ya-Wen ; Carlson, Robert E ; Marcus, Karen L ; Karu, Alexander E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a476t-5b66d8cb15578de8345f7af3312e8e0962dc0a28fbc73a9cdbb84972357f366c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Analytical chemistry</topic><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Binding, Competitive</topic><topic>Cells, Cultured</topic><topic>Chemistry</topic><topic>Cross Reactions</topic><topic>Exact sciences and technology</topic><topic>Haptens - chemistry</topic><topic>Hybridomas</topic><topic>Immunoenzyme Techniques</topic><topic>Mice</topic><topic>Miscellaneous</topic><topic>PCB</topic><topic>Polychlorinated biphenyls</topic><topic>Polychlorinated Biphenyls - analysis</topic><topic>Polychlorinated Biphenyls - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chiu, Ya-Wen</creatorcontrib><creatorcontrib>Carlson, Robert E</creatorcontrib><creatorcontrib>Marcus, Karen L</creatorcontrib><creatorcontrib>Karu, Alexander E</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chiu, Ya-Wen</au><au>Carlson, Robert E</au><au>Marcus, Karen L</au><au>Karu, Alexander E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Monoclonal Immunoassay for the Coplanar Polychlorinated Biphenyls</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>1995-11-01</date><risdate>1995</risdate><volume>67</volume><issue>21</issue><spage>3829</spage><epage>3839</epage><pages>3829-3839</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>Polychlorinated biphenyls (PCBs) are ubiquitous environmental pollutants with diverse toxic, teratogenic, reproductive, immunotoxic, and tumorigenic effects. Three of the least abundant of the 209 PCB isomers (congeners) are the most toxic and most difficult to quantify. These are 3,4,3',4'-tetrachlorobiphenyl, 3,4,3',4',5'-pentachlorobiphenyl, and 3,4,5,3',4',5'-hexachlorobiphenyl (IU-PAC No. 77, 126, and 169, respectively). An immunizing hapten was designed to retain the 3,4,3',4' chlorine-substitution pattern and coplanarity characteristic of these toxic congeners. The optimal competitors for immunoassay were weaker binding distinctive single-ring fragments of the PCBs. A monoclonal antibody designated S2B1 was derived and used in direct (antibody-capture) competitive enzyme immunoassays (EIAs). The EIAs are highly specific for non-ortho-substituted congeners and do not recognize the more prevalent but much less toxic noncoplanar PCB congeners or 2,3,7,8-tetrachlorodibenzo-p-dioxin, 2,3,7,8-tetrachlorodibenzofuran, or dichlorobenzenes. Hapten and competitor design for this assay suggests a basis for development of sensitive EIAs for other classes of PCB congeners.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>8633754</pmid><doi>10.1021/ac00117a003</doi><tpages>11</tpages></addata></record> |
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| ispartof | Analytical chemistry (Washington), 1995-11, Vol.67 (21), p.3829-3839 |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Analytical chemistry Animals Antibodies, Monoclonal - immunology Binding, Competitive Cells, Cultured Chemistry Cross Reactions Exact sciences and technology Haptens - chemistry Hybridomas Immunoenzyme Techniques Mice Miscellaneous PCB Polychlorinated biphenyls Polychlorinated Biphenyls - analysis Polychlorinated Biphenyls - metabolism |
| title | A Monoclonal Immunoassay for the Coplanar Polychlorinated Biphenyls |
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