Physiological and comparative proteomic analyses of saline-alkali NaHCO₃-responses in leaves of halophyte Puccinellia tenuiflora
Aims Soil alkalization imposes severe ion toxicity, osmotic stress, and high pH stress to plants, inhibiting their growth and productivity. NaHCO 3 is a main component of alkaline soil. However, knowledge of the NaHCO 3 -responsive proteomic pattern of alkaligrass is still lacking. Alkaligrass ( Puc...
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| Veröffentlicht in: | Plant and soil 2019-04, Vol.437 (1/2), p.137-158 |
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| creator | Yin, Zepeng Zhang, Heng Zhao, Qi Yoo, Mi-Jeong Zhu, Ning Yu, Jianlan Yu, Juanjuan Guo, Siyi Miao, Yuchen Chen, Sixue Qin, Zhi Dai, Shaojun |
| description | Aims
Soil alkalization imposes severe ion toxicity, osmotic stress, and high pH stress to plants, inhibiting their growth and productivity. NaHCO
3
is a main component of alkaline soil. However, knowledge of the NaHCO
3
-responsive proteomic pattern of alkaligrass is still lacking. Alkaligrass (
Puccinellia tenuiflora
) is a monocotyledonous halophyte pasture widely distributed in the Songnen Plain in Northeastern China. This study aims to investigate the NaHCO
3
-responsive molecular mechanisms in the alkaligrass plants.
Methods
An integrative approach including photosynthetic and redox physiology, and comparative proteomics was used.
Results
NaHCO
3
decreased photosynthesis, but increased nonphotochemical quenching, increased membrane electrolyte leakage of alkaligrass, and increased proline and glycine betaine concentrations in leaves. In addition, the NaHCO
3
stress increased Na
+
concentration and decreased K
+
/Na
+
ratio in leaves, while Ca
2+
and Mg
2+
concentrations were maintained, contributing to signaling and homeostasis of ion and enzyme activity. Furthermore, O
2
−
generation rate and H
2
O
2
concentration were increased, and the activities of ten antioxidant enzymes and antioxidant concentrations were changed in response to the NaHCO
3
stress. Proteomics revealed 90 NaHCO
3
-responsive proteins, 54% of which were localized in chloroplasts. They were mainly involved in signaling, photosynthesis, stress and defense, carbohydrate and energy metabolism, as well as protein synthesis, processing and turnover. Some protein abundances did not correlate well with their activities, implying that the enzyme activities were affected by NaHCO
3
-induced post-translational modifications.
Conclusions
To cope with the NaHCO
3
stress, alkaligrass deployed multiple strategies, including triggering phospholipase D (PLD)-mediated Ca
2+
signaling pathways, enhancing diverse reactive oxygen species (ROS) scavenging pathways, and regulating chloroplast protein synthesis and processing. |
| doi_str_mv | 10.1007/s11104-019-03955-9 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_journals_2177847526</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>48703740</jstor_id><sourcerecordid>48703740</sourcerecordid><originalsourceid>FETCH-LOGICAL-c341t-e3bc74a369a80a3673bf90f81841ad9489dedda7ceffc5e1a101e93a3b8048ae3</originalsourceid><addsrcrecordid>eNp9kMFuEzEQhi1EJULpCyAhWeJsGMfeeH1EEbRIFe2BSr1ZE-9s4-CsF3tTKdfypjwJDovgxmlm5O8ba37GXkt4JwHM-yKlBC1AWgHKNo2wz9hCNkaJBtTqOVsAqKUAY-9fsJel7OA0y9WCPd1ujyWkmB6Cx8hx6LhP-xEzTuGR-JjTRGkffH3BeCxUeOp5wRgGEhi_1YZ_wav1zc-nHyJTGdNwYsLAI-HjTG8xpnF7nIjfHryvYowB-UTDIfQxZXzFznqMhS7-1HN29-nj1_WVuL65_Lz-cC280nISpDbeaFQriy3UYtSmt9C3stUSO6tb21HXofHU974hiRIkWYVq04JukdQ5ezvvrUd9P1CZ3C4dcj2ruKU0ptWmWa4qtZwpn1MpmXo35rDHfHQS3ClrN2ftatbud9bOVknNUqnw8ED53-r_Wm9ma1emlP_-o1sDymhQvwBAuI-I</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2177847526</pqid></control><display><type>article</type><title>Physiological and comparative proteomic analyses of saline-alkali NaHCO₃-responses in leaves of halophyte Puccinellia tenuiflora</title><source>JSTOR Archive Collection A-Z Listing</source><source>SpringerLink Journals - AutoHoldings</source><creator>Yin, Zepeng ; Zhang, Heng ; Zhao, Qi ; Yoo, Mi-Jeong ; Zhu, Ning ; Yu, Jianlan ; Yu, Juanjuan ; Guo, Siyi ; Miao, Yuchen ; Chen, Sixue ; Qin, Zhi ; Dai, Shaojun</creator><creatorcontrib>Yin, Zepeng ; Zhang, Heng ; Zhao, Qi ; Yoo, Mi-Jeong ; Zhu, Ning ; Yu, Jianlan ; Yu, Juanjuan ; Guo, Siyi ; Miao, Yuchen ; Chen, Sixue ; Qin, Zhi ; Dai, Shaojun</creatorcontrib><description>Aims
Soil alkalization imposes severe ion toxicity, osmotic stress, and high pH stress to plants, inhibiting their growth and productivity. NaHCO
3
is a main component of alkaline soil. However, knowledge of the NaHCO
3
-responsive proteomic pattern of alkaligrass is still lacking. Alkaligrass (
Puccinellia tenuiflora
) is a monocotyledonous halophyte pasture widely distributed in the Songnen Plain in Northeastern China. This study aims to investigate the NaHCO
3
-responsive molecular mechanisms in the alkaligrass plants.
Methods
An integrative approach including photosynthetic and redox physiology, and comparative proteomics was used.
Results
NaHCO
3
decreased photosynthesis, but increased nonphotochemical quenching, increased membrane electrolyte leakage of alkaligrass, and increased proline and glycine betaine concentrations in leaves. In addition, the NaHCO
3
stress increased Na
+
concentration and decreased K
+
/Na
+
ratio in leaves, while Ca
2+
and Mg
2+
concentrations were maintained, contributing to signaling and homeostasis of ion and enzyme activity. Furthermore, O
2
−
generation rate and H
2
O
2
concentration were increased, and the activities of ten antioxidant enzymes and antioxidant concentrations were changed in response to the NaHCO
3
stress. Proteomics revealed 90 NaHCO
3
-responsive proteins, 54% of which were localized in chloroplasts. They were mainly involved in signaling, photosynthesis, stress and defense, carbohydrate and energy metabolism, as well as protein synthesis, processing and turnover. Some protein abundances did not correlate well with their activities, implying that the enzyme activities were affected by NaHCO
3
-induced post-translational modifications.
Conclusions
To cope with the NaHCO
3
stress, alkaligrass deployed multiple strategies, including triggering phospholipase D (PLD)-mediated Ca
2+
signaling pathways, enhancing diverse reactive oxygen species (ROS) scavenging pathways, and regulating chloroplast protein synthesis and processing.</description><identifier>ISSN: 0032-079X</identifier><identifier>EISSN: 1573-5036</identifier><identifier>DOI: 10.1007/s11104-019-03955-9</identifier><language>eng</language><publisher>Cham: Springer Science + Business Media</publisher><subject>Alkaline soils ; Alkalizing ; Antioxidants ; Biomedical and Life Sciences ; Calcium ions ; Calcium signalling ; Carbohydrate metabolism ; Carbohydrates ; Chloroplasts ; Ecology ; Electrolyte leakage ; Energy metabolism ; Enzymatic activity ; Enzyme activity ; Enzymes ; Glycine ; Glycine betaine ; Halophytes ; Homeostasis ; Hydrogen peroxide ; Leaves ; Life Sciences ; Magnesium ; Metabolism ; Molecular modelling ; Osmotic stress ; Pasture ; Phospholipase ; Phospholipase D ; Photosynthesis ; Physiology ; Plant Physiology ; Plant Sciences ; Post-translation ; Proline ; Protein biosynthesis ; Protein synthesis ; Protein turnover ; Proteins ; Proteomics ; Puccinellia ; Reactive oxygen species ; REGULAR ARTICLE ; Scavenging ; Signal transduction ; Sodium bicarbonate ; Soil Science & Conservation ; Stress concentration ; Toxicity</subject><ispartof>Plant and soil, 2019-04, Vol.437 (1/2), p.137-158</ispartof><rights>Springer Nature Switzerland AG 2019</rights><rights>Plant and Soil is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c341t-e3bc74a369a80a3673bf90f81841ad9489dedda7ceffc5e1a101e93a3b8048ae3</citedby><cites>FETCH-LOGICAL-c341t-e3bc74a369a80a3673bf90f81841ad9489dedda7ceffc5e1a101e93a3b8048ae3</cites><orcidid>0000-0001-8063-6946</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/48703740$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/48703740$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27923,27924,41487,42556,51318,58016,58249</link.rule.ids></links><search><creatorcontrib>Yin, Zepeng</creatorcontrib><creatorcontrib>Zhang, Heng</creatorcontrib><creatorcontrib>Zhao, Qi</creatorcontrib><creatorcontrib>Yoo, Mi-Jeong</creatorcontrib><creatorcontrib>Zhu, Ning</creatorcontrib><creatorcontrib>Yu, Jianlan</creatorcontrib><creatorcontrib>Yu, Juanjuan</creatorcontrib><creatorcontrib>Guo, Siyi</creatorcontrib><creatorcontrib>Miao, Yuchen</creatorcontrib><creatorcontrib>Chen, Sixue</creatorcontrib><creatorcontrib>Qin, Zhi</creatorcontrib><creatorcontrib>Dai, Shaojun</creatorcontrib><title>Physiological and comparative proteomic analyses of saline-alkali NaHCO₃-responses in leaves of halophyte Puccinellia tenuiflora</title><title>Plant and soil</title><addtitle>Plant Soil</addtitle><description>Aims
Soil alkalization imposes severe ion toxicity, osmotic stress, and high pH stress to plants, inhibiting their growth and productivity. NaHCO
3
is a main component of alkaline soil. However, knowledge of the NaHCO
3
-responsive proteomic pattern of alkaligrass is still lacking. Alkaligrass (
Puccinellia tenuiflora
) is a monocotyledonous halophyte pasture widely distributed in the Songnen Plain in Northeastern China. This study aims to investigate the NaHCO
3
-responsive molecular mechanisms in the alkaligrass plants.
Methods
An integrative approach including photosynthetic and redox physiology, and comparative proteomics was used.
Results
NaHCO
3
decreased photosynthesis, but increased nonphotochemical quenching, increased membrane electrolyte leakage of alkaligrass, and increased proline and glycine betaine concentrations in leaves. In addition, the NaHCO
3
stress increased Na
+
concentration and decreased K
+
/Na
+
ratio in leaves, while Ca
2+
and Mg
2+
concentrations were maintained, contributing to signaling and homeostasis of ion and enzyme activity. Furthermore, O
2
−
generation rate and H
2
O
2
concentration were increased, and the activities of ten antioxidant enzymes and antioxidant concentrations were changed in response to the NaHCO
3
stress. Proteomics revealed 90 NaHCO
3
-responsive proteins, 54% of which were localized in chloroplasts. They were mainly involved in signaling, photosynthesis, stress and defense, carbohydrate and energy metabolism, as well as protein synthesis, processing and turnover. Some protein abundances did not correlate well with their activities, implying that the enzyme activities were affected by NaHCO
3
-induced post-translational modifications.
Conclusions
To cope with the NaHCO
3
stress, alkaligrass deployed multiple strategies, including triggering phospholipase D (PLD)-mediated Ca
2+
signaling pathways, enhancing diverse reactive oxygen species (ROS) scavenging pathways, and regulating chloroplast protein synthesis and processing.</description><subject>Alkaline soils</subject><subject>Alkalizing</subject><subject>Antioxidants</subject><subject>Biomedical and Life Sciences</subject><subject>Calcium ions</subject><subject>Calcium signalling</subject><subject>Carbohydrate metabolism</subject><subject>Carbohydrates</subject><subject>Chloroplasts</subject><subject>Ecology</subject><subject>Electrolyte leakage</subject><subject>Energy metabolism</subject><subject>Enzymatic activity</subject><subject>Enzyme activity</subject><subject>Enzymes</subject><subject>Glycine</subject><subject>Glycine betaine</subject><subject>Halophytes</subject><subject>Homeostasis</subject><subject>Hydrogen peroxide</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Magnesium</subject><subject>Metabolism</subject><subject>Molecular modelling</subject><subject>Osmotic stress</subject><subject>Pasture</subject><subject>Phospholipase</subject><subject>Phospholipase D</subject><subject>Photosynthesis</subject><subject>Physiology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Post-translation</subject><subject>Proline</subject><subject>Protein biosynthesis</subject><subject>Protein synthesis</subject><subject>Protein turnover</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Puccinellia</subject><subject>Reactive oxygen species</subject><subject>REGULAR ARTICLE</subject><subject>Scavenging</subject><subject>Signal transduction</subject><subject>Sodium bicarbonate</subject><subject>Soil Science & Conservation</subject><subject>Stress concentration</subject><subject>Toxicity</subject><issn>0032-079X</issn><issn>1573-5036</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kMFuEzEQhi1EJULpCyAhWeJsGMfeeH1EEbRIFe2BSr1ZE-9s4-CsF3tTKdfypjwJDovgxmlm5O8ba37GXkt4JwHM-yKlBC1AWgHKNo2wz9hCNkaJBtTqOVsAqKUAY-9fsJel7OA0y9WCPd1ujyWkmB6Cx8hx6LhP-xEzTuGR-JjTRGkffH3BeCxUeOp5wRgGEhi_1YZ_wav1zc-nHyJTGdNwYsLAI-HjTG8xpnF7nIjfHryvYowB-UTDIfQxZXzFznqMhS7-1HN29-nj1_WVuL65_Lz-cC280nISpDbeaFQriy3UYtSmt9C3stUSO6tb21HXofHU974hiRIkWYVq04JukdQ5ezvvrUd9P1CZ3C4dcj2ruKU0ptWmWa4qtZwpn1MpmXo35rDHfHQS3ClrN2ftatbud9bOVknNUqnw8ED53-r_Wm9ma1emlP_-o1sDymhQvwBAuI-I</recordid><startdate>20190401</startdate><enddate>20190401</enddate><creator>Yin, Zepeng</creator><creator>Zhang, Heng</creator><creator>Zhao, Qi</creator><creator>Yoo, Mi-Jeong</creator><creator>Zhu, Ning</creator><creator>Yu, Jianlan</creator><creator>Yu, Juanjuan</creator><creator>Guo, Siyi</creator><creator>Miao, Yuchen</creator><creator>Chen, Sixue</creator><creator>Qin, Zhi</creator><creator>Dai, Shaojun</creator><general>Springer Science + Business Media</general><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SN</scope><scope>7ST</scope><scope>7T7</scope><scope>7X2</scope><scope>88A</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>SOI</scope><orcidid>https://orcid.org/0000-0001-8063-6946</orcidid></search><sort><creationdate>20190401</creationdate><title>Physiological and comparative proteomic analyses of saline-alkali NaHCO₃-responses in leaves of halophyte Puccinellia tenuiflora</title><author>Yin, Zepeng ; Zhang, Heng ; Zhao, Qi ; Yoo, Mi-Jeong ; Zhu, Ning ; Yu, Jianlan ; Yu, Juanjuan ; Guo, Siyi ; Miao, Yuchen ; Chen, Sixue ; Qin, Zhi ; Dai, Shaojun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c341t-e3bc74a369a80a3673bf90f81841ad9489dedda7ceffc5e1a101e93a3b8048ae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Alkaline soils</topic><topic>Alkalizing</topic><topic>Antioxidants</topic><topic>Biomedical and Life Sciences</topic><topic>Calcium ions</topic><topic>Calcium signalling</topic><topic>Carbohydrate metabolism</topic><topic>Carbohydrates</topic><topic>Chloroplasts</topic><topic>Ecology</topic><topic>Electrolyte leakage</topic><topic>Energy metabolism</topic><topic>Enzymatic activity</topic><topic>Enzyme activity</topic><topic>Enzymes</topic><topic>Glycine</topic><topic>Glycine betaine</topic><topic>Halophytes</topic><topic>Homeostasis</topic><topic>Hydrogen peroxide</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>Magnesium</topic><topic>Metabolism</topic><topic>Molecular modelling</topic><topic>Osmotic stress</topic><topic>Pasture</topic><topic>Phospholipase</topic><topic>Phospholipase D</topic><topic>Photosynthesis</topic><topic>Physiology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Post-translation</topic><topic>Proline</topic><topic>Protein biosynthesis</topic><topic>Protein synthesis</topic><topic>Protein turnover</topic><topic>Proteins</topic><topic>Proteomics</topic><topic>Puccinellia</topic><topic>Reactive oxygen species</topic><topic>REGULAR ARTICLE</topic><topic>Scavenging</topic><topic>Signal transduction</topic><topic>Sodium bicarbonate</topic><topic>Soil Science & Conservation</topic><topic>Stress concentration</topic><topic>Toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yin, Zepeng</creatorcontrib><creatorcontrib>Zhang, Heng</creatorcontrib><creatorcontrib>Zhao, Qi</creatorcontrib><creatorcontrib>Yoo, Mi-Jeong</creatorcontrib><creatorcontrib>Zhu, Ning</creatorcontrib><creatorcontrib>Yu, Jianlan</creatorcontrib><creatorcontrib>Yu, Juanjuan</creatorcontrib><creatorcontrib>Guo, Siyi</creatorcontrib><creatorcontrib>Miao, Yuchen</creatorcontrib><creatorcontrib>Chen, Sixue</creatorcontrib><creatorcontrib>Qin, Zhi</creatorcontrib><creatorcontrib>Dai, Shaojun</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Ecology Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Agricultural Science Collection</collection><collection>Biology Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Plant and soil</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yin, Zepeng</au><au>Zhang, Heng</au><au>Zhao, Qi</au><au>Yoo, Mi-Jeong</au><au>Zhu, Ning</au><au>Yu, Jianlan</au><au>Yu, Juanjuan</au><au>Guo, Siyi</au><au>Miao, Yuchen</au><au>Chen, Sixue</au><au>Qin, Zhi</au><au>Dai, Shaojun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Physiological and comparative proteomic analyses of saline-alkali NaHCO₃-responses in leaves of halophyte Puccinellia tenuiflora</atitle><jtitle>Plant and soil</jtitle><stitle>Plant Soil</stitle><date>2019-04-01</date><risdate>2019</risdate><volume>437</volume><issue>1/2</issue><spage>137</spage><epage>158</epage><pages>137-158</pages><issn>0032-079X</issn><eissn>1573-5036</eissn><abstract>Aims
Soil alkalization imposes severe ion toxicity, osmotic stress, and high pH stress to plants, inhibiting their growth and productivity. NaHCO
3
is a main component of alkaline soil. However, knowledge of the NaHCO
3
-responsive proteomic pattern of alkaligrass is still lacking. Alkaligrass (
Puccinellia tenuiflora
) is a monocotyledonous halophyte pasture widely distributed in the Songnen Plain in Northeastern China. This study aims to investigate the NaHCO
3
-responsive molecular mechanisms in the alkaligrass plants.
Methods
An integrative approach including photosynthetic and redox physiology, and comparative proteomics was used.
Results
NaHCO
3
decreased photosynthesis, but increased nonphotochemical quenching, increased membrane electrolyte leakage of alkaligrass, and increased proline and glycine betaine concentrations in leaves. In addition, the NaHCO
3
stress increased Na
+
concentration and decreased K
+
/Na
+
ratio in leaves, while Ca
2+
and Mg
2+
concentrations were maintained, contributing to signaling and homeostasis of ion and enzyme activity. Furthermore, O
2
−
generation rate and H
2
O
2
concentration were increased, and the activities of ten antioxidant enzymes and antioxidant concentrations were changed in response to the NaHCO
3
stress. Proteomics revealed 90 NaHCO
3
-responsive proteins, 54% of which were localized in chloroplasts. They were mainly involved in signaling, photosynthesis, stress and defense, carbohydrate and energy metabolism, as well as protein synthesis, processing and turnover. Some protein abundances did not correlate well with their activities, implying that the enzyme activities were affected by NaHCO
3
-induced post-translational modifications.
Conclusions
To cope with the NaHCO
3
stress, alkaligrass deployed multiple strategies, including triggering phospholipase D (PLD)-mediated Ca
2+
signaling pathways, enhancing diverse reactive oxygen species (ROS) scavenging pathways, and regulating chloroplast protein synthesis and processing.</abstract><cop>Cham</cop><pub>Springer Science + Business Media</pub><doi>10.1007/s11104-019-03955-9</doi><tpages>22</tpages><orcidid>https://orcid.org/0000-0001-8063-6946</orcidid></addata></record> |
| fulltext | fulltext |
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| ispartof | Plant and soil, 2019-04, Vol.437 (1/2), p.137-158 |
| issn | 0032-079X 1573-5036 |
| language | eng |
| recordid | cdi_proquest_journals_2177847526 |
| source | JSTOR Archive Collection A-Z Listing; SpringerLink Journals - AutoHoldings |
| subjects | Alkaline soils Alkalizing Antioxidants Biomedical and Life Sciences Calcium ions Calcium signalling Carbohydrate metabolism Carbohydrates Chloroplasts Ecology Electrolyte leakage Energy metabolism Enzymatic activity Enzyme activity Enzymes Glycine Glycine betaine Halophytes Homeostasis Hydrogen peroxide Leaves Life Sciences Magnesium Metabolism Molecular modelling Osmotic stress Pasture Phospholipase Phospholipase D Photosynthesis Physiology Plant Physiology Plant Sciences Post-translation Proline Protein biosynthesis Protein synthesis Protein turnover Proteins Proteomics Puccinellia Reactive oxygen species REGULAR ARTICLE Scavenging Signal transduction Sodium bicarbonate Soil Science & Conservation Stress concentration Toxicity |
| title | Physiological and comparative proteomic analyses of saline-alkali NaHCO₃-responses in leaves of halophyte Puccinellia tenuiflora |
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